《楚辭疏》訓詁硏究

張學城(Zhang Xuecheng),薄迎迎(Bo Yingying) 중국어문학연구회 2017 중국어문학논집 Vol.0 No.106

The “Chuci Shu” of Lu Shiyong is quite famous in the late Ming Dynasty for its study on the Chuci. When annotated, Lu Shiyong strived to concise, so that the book can be “easy to beginner”. Many features in this book can make people refresh. The article introduces the exegetical characteristics of Lu Shi Yong. Exegetical features include three aspects: pay attention to the old note, widely absorb views of other people; courage to innovate; attach importance to culture. These characteristics run through the exegetical study of "Chuci Shu", which clearly reflects Lu Shiyong’s attitude.The article also introduces the shortcomings of the exegetical study of "Chuci Shu", The inadequacies include three aspects: phonetic multi-leaf sound; many discourse from the sentence, less words; inaccurate citation.

Behaviour of Endplate Joints Subjected to Elevated Temperature after Cyclic Loads

Zhen Guo,Xuecheng Zhang,Xingzhi Jia 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.11

This paper presents the performance of an end-plate joint at elevated temperature after cyclic loads. Prime interest of the end-plate joints, subjected to elevated temperature, lies on the effect of three kinds of external conditions: monotonic loads, cyclic loads and local damages. Parametric studies have been systematically conducted using simplified models. The Behaviour of endplate joints subjected to high temperature is examined under two levels of damage caused to the structure, i.e., deformation damage and fracture damage under earthquake. Numerical results indicate that dead loads on beam and material properties of endplate joints are still playing a significant role in fire resistance. Under only deformation damage, the dominance of joints’ resistance to fire is still primarily the property of the material itself. Once experiencing fracture damage, for instance endplates fracture and bolts breakage, the behaviour of joints will degrade severely in post-earthquake fire. The results of this study demonstrate that the deformation damages has limited effect on the endplate joint at elevated temperature after an earthquake. The achieved results can be adopted during the design stage in order to minimize the probability of collapse in the fire.

Fire Resistances of Restrained Steel Beams Subjected to Fire Loads

Zhen Guo,Rui Gao,Xuecheng Zhang,Xingzhi Jia 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.8

Conventional fire loads and fire resistances of a steel beam still lack an adequate correlation. This paper has established therelationship between the responses of restrained steel beams and fire actions by using a new definition of fire resistances and a newexpression of fire loads. By using reduction factors of elastic modulus and yield strengths, has presented three critical equations topredict the limit state of a restrained steel beam in a fire. Based on these equations and the heat transfer formula, the paper provided anew definition of fire resistances. By using the heat release rate and effective rate coefficient of thermal absorption, a new expressionof fire loads has been argued. Compared with tests, the proposed approach in this paper is in good agreement with the measuredvalues in tests. The results show that the new fire resistances could be able to reflect the facts of heat transfers and duration time. Incontrast to conventional fire loads, the new fire loads are more efficient to indicate a fire load.

Ultrastructural changes of Haematococcus pluvialis (Chlorophyta) in process of astaxanthin accumulation and cell damage under condition of high light with acetate

He, Bangxiang,Hou, Lulu,Zhang, Feng,Cong, Xiaomei,Wang, Zhendong,Guo, Yalin,Shi, Jiawei,Jiang, Ming,Zhang, Xuecheng,Zang, Xiaonan The Korean Society of Phycology 2020 ALGAE Vol.35 No.3

Haematococcus pluvialis is a commercial microalga that can produce high quantities of astaxanthin. Under induced conditions, some important changes in the subcellular structures related to astaxanthin accumulation were observable. For example, a large number of astaxanthin granules, oil structures and starch granules appeared in the thick-walled cells; Astaxanthin granules gradually dissolved into the oil structures and spread throughout the entire cell with the fusion and diffusion process of oil structures during the middle and late stages of induction; The plastoglobules were closed to the newly formed structures, and some plastoglobules would abnormally increase in size under stress. Based on observations of cell damage, the degradation of membrane structures, such as chloroplasts, was found to be the primary form of damage during the early stage of induction. During the middle stage of induction, some transparent holes were exposed in the dissolving astaxanthin granules in the cytoplasm. In thick-walled cells, these transparent holes were covered by oil substances dissolving astaxanthin, thereby avoiding further damage to cells. Given the relatively few oil structures, in non-thick-walled cells, the transparent holes expanded to form multiple transparent areas, eventually resulting in the rupture and death of cells. These results suggested that the high level of synthesis and the wide range diffusion of oil explained the expansion of astaxanthin in H. pluvialis.

Lead ion detection using glutathione-functionalized aluminum gallium nitride/gallium nitride high-electron-mobility transistors

Dong Xiaohu,Jiang Xuecheng,Gu Yan,Wei Chunlei,Xie Zhijian,Zhang Qi,Qian Weiying,Zhang Xiangyang,Zhu Chun,Lu Naiyan,Chen Guoqing,Yang Guofeng 한국물리학회 2023 Current Applied Physics Vol.50 No.-

This work presents a new approach for lead ion detection (Pb2+) using an aluminum gallium nitride/gallium nitride (AlGaN/GaN) high-electron-mobility transistor (HEMT) sensor. The AlGaN/GaN HEMT structure of the sensor was realized by functionalizing the gate area with glutathione (GSH). The crystalline and surface qualities of the AlGaN film were measured through X-ray diffraction and atomic force microscopy. The response of the sensor was measured in terms of the source–drain current with varying concentrations of Pb2+ ions at a fixed drain-to-source voltage. The sensitivity of the sensor was 29.3 μA/(mg/L), and it exhibited high selectivity toward Pb2+. The results show that using the GSH-functionalized AlGaN/GaN HEMT sensor is a promising strategy for Pb2+ ion detection.

PspAG97A: A Halophilic α-Glucoside Hydrolase with Wide Substrate Specificity from Glycoside Hydrolase Family 97^s

( Wei Li ),( Han Fan ),( Chao He ),( Xuecheng Zhang ),( Xiaotang Wang ),( Jing Yuan ),( Zemin Fang ),( Wei Fang ),( Yazhong Xiao ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.11

A novel α-glucoside hydrolase (named PspAG97A) from glycoside hydrolase family 97 (GH97) was cloned from the deep-sea bacterium Pseudoalteromonas sp. K8, which was screened from the sediment of Kongsfjorden. Sequence analysis showed that PspAG97A belonged to GH97, and shared 41% sequence identity with the characterized α-glucosidase BtGH97a. PspAG97A possessed three key catalytically related glutamate residues. Mutation of the glutamate residues indicated that PspAG97A belonged to the inverting subfamily of GH97. PspAG97A showed significant reversibility against changes in salt concentration. It exhibited halophilic ability and improved thermostability in NaCl solution, with maximal activity at 1.0 M NaCl/KCl, and retained more than 80% activity at NaCl concentrations ranging from 0.8 to 2.0 M for over 50 h. Furthermore, PspAG97A hydrolyzed not only α-1,4-glucosidic linkage, but also α-1,6- and α-1,2-glucosidic linkages. Interestingly, PspAG97A possessed high catalytic efficiency for long-chain substrates with α-1,6-linkage. These characteristics are clearly different from other known α-glucoside hydrolases in GH97, implying that PspAG97A is a unique α-glucoside hydrolase of GH97.

Role of Interleukin-4 (IL-4) in Respiratory Infection and Allergy Caused by Early-Life Chlamydia Infection

( Shujun Li ),( Lijuan Wang ),( Yulong Zhang ),( Long Ma ),( Jing Zhang ),( Jianbing Zu ),( Xuecheng Wu ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.8

Chlamydia pneumoniae is a type of pathogenic gram-negative bacteria that causes various respiratory tract infections including asthma. Chlamydia species infect humans and cause respiratory infection by rupturing the lining of the respiratory which includes the throat, lungs and windpipe. Meanwhile, the function of interleukin-4 (IL-4) in Ch. pneumoniae respiratory infection and its association with the development of airway hyperresponsiveness (AHR) in adulthood and causing allergic airway disease (AAD) are not understood properly. We therefore investigated the role of IL-4 in respiratory infection and allergy caused by early life Chlamydia infection. In this study, Ch. pneumonia strain was propagated and cultured in HEp-2 cells according to standard protocol and infant C57BL/6 mice around 3-4 weeks old were infected to study the role of IL-4 in respiratory infection and allergy caused by early life Chlamydia infection. We observed that IL-4 is linked with Chlamydia respiratory infection and its absence lowers respiratory infection. IL-4R α2 is also responsible for controlling the IL-4 signaling pathway and averts the progression of infection and inflammation. Furthermore, the IL-4 signaling pathway also influences infection-induced AHR and aids in increasing AAD severity. STAT6 also promotes respiratory infection caused by Ch. pneumoniae and further enhanced its downstream process. Our study concluded that IL-4 is a potential target for preventing infection-induced AHR and severe asthma.

Optimum Conditions for Transformation of Synechocystis sp. PCC 6803

Xiaonan Zang,Bin Liu,Shunmei Liu,K.K.I.U. Arunakumara,Xuecheng Zhang 한국미생물학회 2007 The journal of microbiology Vol.45 No.3

This study was conducted to determine the optimal conditions for introduction of exogenous DNA into Synechocystis sp. PCC 6803. Of the three transformation techniques studied, electroporation, ultrasonic transformation and natural transformation, natural transformation showed the highest efficiency. Additionally, this study demonstrated that the higher plasmid concentration and longer homologous recombining fragments resulted in a greater number of transformants. For successful transformation, the lowest concentration of plasmid was 0.02 μg/ml, and the shortest homologous recombining fragment was 0.2 kb. Use of Synechocystis sp. PCC 6803 in the logarithmic growth phase resulted in two-fold higher transformation rate than that of the same organism when cells in the latent phase or the plateau phase were used for transformation. Pretreatment of the host strain, Synechocystis sp. PCC 6803, with EDTA (2 mM) for two days prior to transformation increased the transformation efficiency by 23%. Additionally, incubation of the cells and DNA for 5 h under light conditions increased the transformation efficiency by two orders of magnitude. Moreover, recovery treatment of the cells before they were plated onto antibiotic medium also increased the transformation efficiency.

Optimum Conditions for Transformation of Synechocystis sp. PCC 6803

Zang, Xiaonan,Liu, Bin,Liu, Shunmei,Arunakumara, K.K.I.U.,Zhang, Xuecheng The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.3

This study was conducted to determine the optimal conditions for introduction of exogenous DNA into Synechocystis sp. PCC 6803. Of the three transformation techniques studied, electroporation, ultrasonic transformation and natural transformation, natural transformation showed the highest efficiency. Additionally, this study demonstrated that the higher plasmid concentration and longer homologous recombining fragments resulted in a greater number of transformants. For successful transformation, the lowest concentration of plasmid was $0.02\;{\mu}g/ml$, and the shortest homologous recombining fragment was 0.2 kb. Use of Synechocystis sp. PCC 6803 in the logarithmic growth phase resulted in two-fold higher transformation rate than that of the same organism when cells in the latent phase or the plateau phase were used for transformation. Pretreatment of the host strain, Synechocystis sp. PCC 6803, with EDTA (2 mM) for two days prior to transformation increased the transformation efficiency by 23%. Additionally, incubation of the cells and DNA for 5 h under light conditions increased the transformation efficiency by two orders of magnitude. Moreover, recovery treatment of the cells before they were plated onto antibiotic medium also increased the transformation efficiency.