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Promoter Analysis of Bombyx mori Nucleopolyhedrovirus Ubiquitin Gene
Xu’ai Lin,Yin Chen,Yongzhu Yi,Jie Yan,Zhifang Zhang 한국미생물학회 2008 The journal of microbiology Vol.46 No.4
The aim of this study was to analyze the characteristics of Bombyx mori nucleopolyhedrovirus (BmNPV) ubiquitin gene promoter and the effects of conserved motifs, such as TAAG, TATA, and CAAT, along with baculovirus enhancer homologous region 3 (hr3), on promoter activity. Ubiquitin gene of BmNPV was expressed during the late phase of virus infection. In the presence of viral factors, significant reduction of promoter activity was observed by deletion of -382 to -124 bp upstream of ATG. The fragment between -187 and -383 bp upstream of ATG, including distal TAAG, CAAT motif, and TATA box, could also drive expression of the reporter gene. The mutation of cis-elements TATA boxes and TAAG motifs significantly decreased the promoter’s activity, while CAAT mutations enhanced promoter activity by 2- or 3-fold, as compared with the native promoter. In the presence of BmNPV, hr3, both located downstream of the reporter gene of the same vector, and separate vector, could significantly enhance transcription activity of ubiquitin promoter as compared to the control. We concluded that BmNPV ubiquitin gene might be regulated by dual sets of promoter elements, where TAAG and TATA box may positively regulate the expression of ubiquitin, while CAAT motif functions as a negative regulator. Viral factor(s) play an important role in the co-activation of hr3 and promoter.
Yi, Yi,Qiao, Dairong,Bai, Linhan,Xu, Hui,Li, Ya,Wang, Xiaolin,Cao, Yi The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.2
5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EC 2.5.1.19) is the sixth enzyme in the shikimate pathway which is essential for the synthesis of aromatic amino acids and many secondary metabolites. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide glyphosate. In this study, the Dunaliella salina EPSP synthase gene was cloned by RT-PCR approach. It contains an open reading frame encoding a protein of 514 amino acids with a calculated molecular weight of 54.6 KDa. The derived amino acid sequence showed high homology with other EPSP synthases. The Dunaliella salina EPSP synthase gene was expressed in Escherichia coli and the recombinant EPSP synthase were identified by functional complementation assay.
A Routing Metric to Improve Route Stability in Mobile Wireless Sensor Networks
( Yi-han Xu ),( Yin Wu ),( Jun Song ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.5
The hop count routing metric is widely used in routing protocols of Wireless Sensor Networks (WSNs) due to its simplicity and effectiveness. With a lower hop count route, fewer transmissions are required to send a packet from the source to the destination. This can improve the throughput of a network because fewer transmissions results in less channel contention and interference. Despite this, the hop count routing metric may not be ideal for mobile scenarios where the topology of a network changes constantly and rapidly. In this paper, we propose to increase route stability in mobile WSNs by discovering paths that are more stable during route discoveries using routing metrics. Two routing metrics were proposed, the true beauty of these routing metrics lies in the fact that they can even be used even without specialized hardware such as GPS and other sensors. We implemented the proposed routing metrics in the AODV routing protocol and found that they are highly effective and outperform other stability-based routing metrics and the hop count routing metric.
Yi-Xin Zou,Jia Qiao,Hua-Yuan Zhu,Rui-Nan Lu,Yi Xia,Lei Cao,Wei Wu,Hui Jin,Wen-Jie Liu,Jin-Hua Liang,Jia-Zhu Wu,Li Wang,Lei Fan,Wei Xu,Jian-Yong Li 대한암학회 2019 Cancer Research and Treatment Vol.51 No.2
Purpose Chronic lymphocytic leukemia (CLL) is one of the most frequent type of B-cell chronic lymphoproliferative disorders and chronic inflammation takes part in the development of CLL. However, there has been no valid immune biomarker to predict the prognosis of untreated CLL patients. Materials and Methods In this retrospective study, we analyzed the clinical correlations and prognostic value of albumin-to-fibrinogen ratio (AFR) detected at diagnosis in 191 CLL patients. Results The cut-off value of AFR was 9.7 calculated by X-tile. Patients who were more than 65 years old were often accompanied by low level of AFR (p < 0.001). Survival analysis showed that patients with low level of AFR had shorter overall survival (OS) than patients with high level of AFR (p < 0.001). Multivariate analysis illustrated that AFR had a negative impact on OS (p=0.003) and was independent of parameters involved in CLL international prognostic index and other prognostic markers such as CD38 and ZAP-70. Conclusion These data provide a comprehensive view of AFR and shows that AFR at diagnosis is an adverse prognostic factor in untreated CLL patients.
Xu, Zheng-Yi,Kim, Soo Youn,Hyeon, Do Young,Kim, Dae Heon,Dong, Ting,Park, Youngmin,Jin, Jing Bo,Joo, Se-Hwan,Kim, Seong-Ki,Hong, Jong Chan,Hwang, Daehee,Hwang, Inhwan American Society of Plant Biologists 2013 The Plant cell Vol.25 No.11
<P>This work examines the role of the NAC transcription factor ANAC096, finding that ANAC096 interacts with specific bZIP transcription factors to globally affect abscisic acid–responsive transcription during osmotic and drought stresses.</P>
Yi Xu,Chen Jin,Shubin Qiao,Yongjian Wu,Hongbing Yan,Kefei Dou,Bo Xu,Jingang Yang,Yuejin Yang 대한심장학회 2018 Korean Circulation Journal Vol.48 No.8
Background and Objectives: Over the past decades, transradial approach for percutaneous coronary intervention (PCI) has been increasingly adopted in clinical practice. Women represent a large population who will possibly benefit from PCI, but they are often under-represented in clinical studies. Therefore, the role of TRI in women remains to be further defined. This study sought to compare safety and efficacy for transradial intervention (TRI) and transfemoral intervention (TFI) in women undergoing PCI in China. Methods: The study population consisted of 5,067 women undergoing PCI at Fuwai Hospital, Beijing, China between 2006 and 2011 (TRI: n=4,105, TFI: n=962). Incidence rates of clinical outcomes during hospitalization and at 1-year follow-up were compared between TRI and TFI. In order to minimize potential biases, a 1:1 propensity score matching (PSM) was performed. A total of 899 pairs were matched. Results: Baseline and procedural characteristics were well-balanced between TRI and TFI groups after controlling for confounders using PSM. TRI was associated with reduced major post-PCI bleeding (odds ratio [OR], 0.64; 95% confidence interval [CI], 0.54–0.76; p<0.001) and access site complications (OR, 0.67; 95% CI, 0.61–0.74; p<0.001) after PSM. There was no statistical differences in the incidence rates of major adverse cardiac events (a composite of cardiac death, myocardial infarction, and target vessel revascularization) both during hospitalization and at 1-year follow-up (p>0.05). Conclusions: In this propensity score-based analysis of TRI versus TFI in Chinese women, TRI showed advantages of safety and feasibility over TFI. A wider adoption of TRI in women has the potential to improve outcomes in treatment of coronary artery diseases.
Yi Xu,Kuanjun Fang,Weichao Chen,Xiangwu Zhang,Chunming Zhang 한국섬유공학회 2022 Fibers and polymers Vol.23 No.9
This paper develops a new clean production technology combining atmospheric pressure plasma jets and nanochitosanto prepare strong and durable bio-based coatings for improving the inkjet printing performance of antimicrobial silkfabrics. The key contribution of this study is to introduce green and environmental friendly production methods, avoiding theuse of chemicals and pollution caused by dye hydrolysis in traditional ways. By contradistinctive experiments, we presentdirect empirical evidence that the plasma-chitosan treatment can significantly improve the hydrophilicity of the silk surfaces. Wettability test indicates that the infiltration time of droplet on the surface of modified silk fabric was only 1/12 of the timespent on the untreated sample. The study of reactive dye inkjet printing illustrates that the antibleeding property on modifiedsamples was evidently enhanced. Meanwhile, in respect of color fixation of reactive dyes, there was also an increase in K/Svalue on modified samples, ranging from 76 % to 214 % in color depth of the four-color print blocks, even after gas steamingand washing. Scanning electron microscope (SEM) observation reveals that the surface of silk fibers modified with plasma iscovered with firmer and more uniform chitosan coatings in terms of morphology change. X-ray photoelectron spectroscopy(XPS) analysis verifies that the content of oxygenous groups on the silk fabric processed by plasma-chitosan increased bymore than 42 %. As to the antibacterial effect of nano-chitosan, the photographs of inhibition zone shows that the plasmaprocessing effectively enhanced the durability of chitosan against B. subtilis, and thus prepared long-lasting antibacterial silkfabric. Furthermore, measurements of washing and rubbing color fastness prove that the plasma-chitosan treated fabrics stillhad excellent inkjet printing effect even after 45 cycles of soaping. Taken together, it turns out that such nontoxic,nonpolluting, low energy-consumed chitosan coating combining with plasma can explicitly provide an innovative option forthe production of inkjet printing on natural fabrics.