Microarray Analysis of the Hypoxia-induced Gene Expression Profile in Malignant C6 Glioma Cells

Huang, Xiao-Dong,Wang, Ze-Fen,Dai, Li-Ming,Li, Zhi-Qiang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Hypoxia is commonly featured during glioma growth and plays an important role in the processes underlying tumor progression to increasing malignancy. Here we compared the gene expression profiles of rat C6 malignant glioma cells under normoxic and hypoxic conditions by cDNA microarray analysis. Compared to normoxic culture conditions, 180 genes were up-regulated and 67 genes were down-regulated under hypoxia mimicked by $CoCl_2$ treatment. These differentially expressed genes were involved in mutiple biological functions including development and differentiation, immune and stress response, metabolic process, and cellular physiological response. It was found that hypoxia significantly regulated genes involved in regulation of glycolysis and cell differentiation, as well as intracellular signalling pathways related to Notch and focal adhesion, which are closely associated with tumor malignant growth. These results should facilitate investigation of the role of hypoxia in the glioma development and exploration of therapeutic targets for inhibition of glioma growth.

Fitting the constitution type Ia supernova data with the redshift-binned parametrization method

Huang, Qing-Guo,Li, Miao,Li, Xiao-Dong,Wang, Shuang American Physical Society 2009 PHYSICAL REVIEW D - Vol.80 No.8

A New Orthogonal Signal Generator with DC Offset Rejection for Single-Phase Phase Locked Loops

Huang, Xiaojiang,Dong, Lei,Xiao, Furong,Liao, Xiaozhong The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.1

This paper presents a new orthogonal signals generator (OSG) with DC Offset rejection for implementing a phase locked loop (PLL) in single-phase grid-connected power systems. An adaptive filter (AF) based on the least mean square (LMS) algorithm is used to constitute the OSG in this study. The DC offset in the measured grid voltage signal can be significantly rejected in the developed OSG technique. This generates two pure orthogonal signals that are free from the DC offset. As a result, the DC offset rejection performance of the presented single-phase phase locked loop (SPLL) can be enhanced. A mathematical model of the developed OSG and the principle of the adaptive filter based SPLL (AF-SPLL) are presented in detail. Finally, simulation and experimental results demonstrate the feasibility of the proposed AF-SPLL.

Distributed Dual-rate Consensus Predictive Control of Looper Tension System in Hot Rolling Mills

Xiao-Dong Zhang,Shao-Shu Gao,Xin-Ping Liu,Ting-Pei Huang 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.2

This paper considers a dual-rate distributed predictive control strategy for the looper tension system in hot rolling mills, which is a typical multi-agent system with directed communication topology. First, we establish an interconnected model for looper tension control system and the disturbances from the neighbors are considered effectively. Second, the consensus control protocol is developed based on the proposed control strategy to improve the robustness and stability of the multi-agent, and the sufficient conditions for consensus are developed. We update and implement all the agent controllers sequentially in one output sampling period and begin a new cycle at the next sampling instant, which leads the multi-agent control system is of fast control updating rate and slow output sampling rate. The control inputs of neighbors can be obtained to compensate the coupling effects, and the cooperation of controllers are improved. Finally, simulation results verify the proposed control strategy and corresponding results.

Protocatechuic Acid, a Gut Bacterial Metabolite of Black Raspberries, Inhibits Adenoma Development and Alters Gut Microbiome Profiles in Apc Min/+ Mice

Dong Athena,Lin Chien-Wei,Echeveste Carla Elena,Huang Yi-Wen,Oshima Kiyoko,Yearsley Martha,Chen Xiao,Yu Jianhua,Wang Li-Shu 대한암예방학회 2022 Journal of cancer prevention Vol.27 No.1

Administration of black raspberries (BRBs) and their anthocyanin metabolites, including protocatechuic acid (PCA), has been demonstrated to exert chemopreventive effects against colorectal cancer through alteration of innate immune cell trafficking, modulation of metabolic and inflammatory pathways, etc. Previous research has shown that the gut microbiome is important in the effectiveness of chemoprevention of colorectal cancer. This study aimed to assess the potency of PCA versus BRB dietary administration for colorectal cancer prevention using an ApcMin/+ mouse model and determine how bacterial profiles change in response to PCA and BRBs. A control AIN-76A diet supplemented with 5% BRBs, 500 ppm PCA, or 1,000 ppm PCA was administered to Apc- Min/+ mice. Changes in incidence, polyp number, and polyp size regarding adenomas of the small intestine and colon were assessed after completion of the diet regimen. There were significant decreases in adenoma development by dietary administration of PCA and BRBs in the small intestine and the 5% BRB-supplemented diet in the colon. Pro-inflammatory bacterial profiles were replaced with anti-inflammatory bacteria in all treatments, with the greatest effects in the 5% BRB and 500 ppm PCA-supplemented diets accompanied by decreased COX-2 and prostaglandin E2 levels in colonic mucosa. We further showed that 500 ppm PCA, but not 1,000 ppm PCA, increased IFN-γ and SMAD4 levels in primary cultured human natural killer cells. These results suggest that both BRBs and a lower dose PCA can benefit colorectal cancer patients by inhibiting the growth and proliferation of adenomas and promoting a more favorable gut microbiome condition.

A Pilot Clinical Study to Investigate the Hypomethylating Properties of Freeze-dried Black Raspberries in Patients with Myelodysplastic Syndrome or Myeloproliferative Neoplasm

Dong Athena,Pan Xiaoqing,Lin Chien-Wei,Huang Yi-Wen,Krause Hayden,Pan Pan,Baim Arielle,Thomas Michael J,Chen Xiao,Yu Jianhua,Michaelis Laura,Liu Pengyuan,Wang Li-Shu,Atallah Ehab 대한암예방학회 2022 Journal of cancer prevention Vol.27 No.2

Myelodysplastic syndromes (MDS) and myelodysplastic/myeloproliferative neoplasms (MDS/MPN) are bone marrow disorders characterized by cytopenias and progression to acute myeloid leukemia. Hypomethylating agents (HMAs) are Food and Drug Administration-approved therapies for MDS and MDS/MPN patients. HMAs have improved patients’ survival and quality of life when compared with other therapies. Although HMAs are effective in MDS and MDS/MPN patients, they are associated with significant toxicities that place a large burden on patients. Our goal is to develop a safer and more effective HMA from natural products. We previously reported that black raspberries (BRBs) have hypomethylating effects in the colon, blood, spleen, and bone marrow of mice. In addition, BRBs exert hypomethylating effects in patients with colorectal cancer and familial adenomatous polyposis. In the current study, we conducted a pilot clinical trial to evaluate the hypomethylating effects of BRBs in patients with low-risk MDS or MDS/MPN. Peripheral blood mononuclear cells (PBMCs) were isolated before and after three months of BRB intervention. CD45+ cells were isolated from PBMCs for methylation analysis using a reduced-representation bisulfite sequencing assay. Each patient served as their own matched control, with their measurements assessed before intervention providing a baseline for post-intervention results. Clinically, our data showed that BRBs were well-tolerated with no side effects. When methylation data was combined, BRBs significantly affected methylation levels of 477 promoter regions. Pathway analysis suggests that BRB-induced intragenic hypomethylation drives leukocyte differentiation. A randomized, placebo-controlled clinical trial of BRB use in low-risk MDS or MDS/ MPN patients is warranted.

Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis

Xiao-wei Zhao,Yong-xin Yang,Dong-wei Huang,Guang-long Cheng,Hui-ling Zhao 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3

Cows infected with Escherichia (E.) coli usually experience severe clinical symptoms, including damage to mammary tissues, reduced milkyield, and altered milk composition. In order to investigate the host response to E. coli infection and discover novel markers for mastitistreatment, mammary tissue samples were collected from healthy cows and bovines with naturally occurring severe E. coli mastitis. Changesof mammary tissue proteins were examined using two-dimensional gel electrophoresis and label-free proteomic approaches. A total of 95differentially expressed proteins were identified. Of these, 56 proteins were categorized according to molecular function, cellular component,and biological processes. The most frequent biological processes influenced by the proteins were response to stress, transport, andestablishment of localization. Furthermore, a network analysis of the proteins with altered expression in mammary tissues demonstrated thatthese factors are predominantly involved with binding and structural molecule activities. Vimentin and -enolase were central “functionalhubs” in the network. Based on results from the present study, disease-induced alterations of protein expression in mammary glands andpotential markers for the effective treatment of E. coli mastitis were identified. These data have also helped elucidate defense mechanismsthat protect the mammary glands and promote the pathogenesis of E. coli mastitis.

Role of Blood Pressure Management in Stroke Prevention: A Systematic Review and Network Meta-Analysis of 93 Randomized Controlled Trials

Xiao-Ling Zhong,Yi Dong,Wei Xu,Yu-Yuan Huang,Hui-Fu Wang,Tian-Song Zhang,Li Sun,Lan Tan,Qiang Dong,Jin-Tai Yu 대한뇌졸중학회 2021 Journal of stroke Vol.23 No.1

Background and Purpose: The present study aimed to compare the efficacy and tolerability of different blood pressure (BP)-lowering strategies. Methods: Randomized controlled trials that compared various antihypertensive treatments and stroke outcomes were included. Eligible trials were categorized into three scenarios: single or combination antihypertensive agents against placebos; single or combination agents against other agents; and different BP-lowering targets. The primary efficacy outcome was the risk reduction pertaining to strokes. The tolerability outcome was the withdrawal of drugs, owing to drug-related side effects (PROSPERO registration number CRD42018118454 [20/12/2018]). Results: The present study included 93 trials (average follow-up duration, 3.3 years). In the pairwise analysis, angiotensin-converting enzyme inhibitors (ACEis) and beta-blockers (BBs) were inferior to calcium channel blockers (CCBs) (odds ratio [OR], 1.123; 95% confidence interval [CI], 1.008 to 1.252) (OR, 1.261; 95% CI, 1.116 to 1.425) for stroke prevention, BB was inferior to angiotensin II receptor blockers (ARB) (OR, 1.361; 95% CI, 1.142 to 1.622), and diuretics were superior to ACEi (OR, 0.871; 95% CI, 0.771 to 0.984). The combination of ACEi+CCB was superior to ACEi+diuretic (OR, 0.892; 95% CI, 0.823 to 0.966). The network meta-analysis confirmed that diuretics were superior to BB (OR, 1.34; 95% CI, 1.11 to 1.58), ACEi+diuretic (OR, 1.47; 95% CI, 1.02 to 2.08), BB+CCB (OR, 2.05; 95% CI, 1.05 to 3.79), and renin inhibitors (OR, 1.87; 95% CI, 1.25 to 2.75) for stroke prevention. Regarding the tolerability profile, the pairwise analysis revealed that ACEi was inferior to CCB and less tolerable, compared to the other treatments. Conclusions: Monotherapy using diuretics, CCB, or ARB, and their combinations could be employed as first-line treatments for stroke prevention in terms of efficacy and tolerability.

A Smooth LVRT Control Strategy for Single-Phase Two-Stage Grid-Connected PV Inverters

Xiao, Furong,Dong, Lei,Khahro, Shahnawaz Farhan,Huang, Xiaojiang,Liao, Xiaozhong The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.3

Based on the inherent relationship between dc-bus voltage and grid feeding active power, two dc-bus voltage regulators with different references are adopted for a grid-connected PV inverter operating in both normal grid voltage mode and low grid voltage mode. In the proposed scheme, an additional dc-bus voltage regulator paralleled with maximum power point tracking controller is used to guarantee the reliability of the low voltage ride-through (LVRT) of the inverter. Unlike conventional LVRT strategies, the proposed strategy does not require detecting grid voltage sag fault in terms of realizing LVRT. Moreover, the developed method does not have switching operations. The proposed technique can also enhance the stability of a power system in case of varying environmental conditions during a low grid voltage period. The operation principle of the presented LVRT control strategy is presented in detail, together with the design guidelines for the key parameters. Finally, a 3 kW prototype is built to validate the feasibility of the proposed LVRT strategy.

Identification of Quantitative Trait Loci (QTLs) for Flowering Time Using SSR Marker in Maize under Water Stress

Xiao, Yan-Nong,Li, Xin-Hai,Zhang, Shi-Huang,Wang, Xiang-Dong,Li, Ming-Shun,Zheng, Yong-Lian 한국유전학회 2004 Genes & Genomics Vol.26 No.4

The selection of reduction of anthesis-silking interval (ASI) in maize breeding is an efficient way to develop maize varieties more tolerant to dry growing conditions. Characterization of the quantitative trait loci (QTL) that controlled the flowering time will be helpful for selection in maize breeding. In this study, flowering time of individuals in a 234 F_(2:3) family, derived from the cross between inbred lines X178 and B73, was evaluated under well-watered and water-stressed conditions at the same location. SSR (microsatellite) was used to identify flowering time QTL. The results showed that the broad-sense heritability for male flowering time (MFT), female flowering time (FFT) and ASI were 0.72, 0.72 - 0.74 and 0.40 - 0.42, respectively, and ASI was significantly correlated to FFT. Under water-stressed condition, 9, 6 and 6 QTLs were identified for MFT, FFT and ASI, respectively, and individual QTL accounted for approximately 2.88% - 31.65% of the phenotypic variation. Some QTLs for MFT were mapped overlapping with those for FIT and ASI. One QTL on chromosome 9 (near nc134) had the strongest effect on MFT, FTT and ASI. It was suggested that the epistasis contributed to the phenotypic variation of flowering time.