Translocation and Phosphorylation of Calcyclin Binding Protein during Retinoic Acid-induced Neuronal Differentiation of Neuroblastoma SH-SY5Y Cells

Wu, Jing,Tan, Xinyu,Peng, Xiaozhong,Yuan, Jiangang,Qiang, Boqin Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.4

For better understanding of functions of the Calcyclin Binding Protein (CacyBP) and exploring its possible roles in neuronal differentiation, the subcellular localization of human CacyBP was examined in retinoic acid(RA)-induced and uninduced neuroblastoma SH-SY5Y cells. Immunostaining indicated that CacyBP was present in the cytoplasm of uninduced SH-SY5Y cells, in which the resting $Ca^{2+}$ concentration was relatively lower than that of RA-induced cells. After the RA induction, immunostaining was seen in both the nucleus and cytoplasm. In the RA-induced differentiated SH-SY5Y cells, CacyBP was phosphorylated on serine residue(s), while it existed in a dephosphorylated form in normal (uninduced) cells. Thus, the phosphorylation of CacyBP occurs when it is translocated to the nuclear region. The translocation of CacyBP during the RA-induced differentiation of SH-SY5Y cells suggested that this protein might play a role in neuronal differentiation.

Analysis of a photonic crystal fiber sensor with reuleaux triangle

Pibin Bing,Shichao Huang,Xinyue Guo,Hongtao Zhang,Lian Tan,Zhongyang Li,Jianquan Yao 한국광학회 2019 Current Optics and Photonics Vol.3 No.3

The characteristics of a photonic crystal fiber sensor with reuleaux triangle are studied by using the finite element method. The wavelength sensitivity of the designed optical fiber sensor is related to the arc radius of the reuleaux triangle. Whether the core area is solid or liquid as well as the refractive index of the liquid core contributes to wavelength sensitivity. The simulation results show that larger arc radius leads to higher sensitivity. The sensitivity can be improved by introducing a liquid core, and higher wavelength sensitivity can be achieved with a lower refractive index liquid core. In addition, the specific channel plated with gold film is polished and then analyte is deposited on the film surface, in which case the position of the resonance peak is the same as that of the complete photonic crystal fiber with three analyte channels being filled with analyte. This means that filling process becomes convenient with equivalent performance of designed sensor. The maximum wavelength sensitivity of the sensor is 10200 nm/RIU and the resolution is 9.8 × 10 -6 RIU.

Simulation of Spinal Codes

Danning Feng,Huijun Yue,Xinyue Li,Siyang Tan,Rui Tao,Junbin Lai 한국산학기술학회 2013 SmartCR Vol.3 No.6

Spinal codes are a new kind of adaptive rateless codes. By analyzing the theory, consisting of hash-function encoding, modulation, maximum-likelihood decoding, and puncturing, the data structure was redesigned, and simulation of the whole transmission process was accomplished. The transfer rate was tested with both additive white Gaussian noise channel (AWGN channel) and binary symmetric channel (BSC). The experiment with AWGN demonstrated the rightness of our design and showed that spinal codes are extremely close to the Shannon Limit. What?s more, validity of the data structure was also proven with BSC. These results show that spinal codes are efficient and practical, which will be very useful in the near future.

A Novel Photonic Crystal Fiber Sensor with Three D-shaped Holes Based on Surface Plasmon Resonance

Pibin Bing,Jialei Sui,Shichao Huang,Xinyue Guo,Zhongyang Li,Lian Tan,Jianquan Yao 한국광학회 2019 Current Optics and Photonics Vol.3 No.6

A novel photonic crystal fiber (PCF) sensor with three D-shaped holes based on surface plasmon resonance (SPR) is analyzed in this paper. Three D-shaped holes are filled with the analyte, and the gold film is deposited on the side of three planes. The design of D-shaped holes with outward expansion can effectively solve the uniformity problem of metallized nano-coating, it is beneficial to the filling of the analyte and is convenient for real-time measurement of the analyte. Compared with the hexagonal lattice structure, the triangular arrangement of the clad air holes can significantly reduce the transmission loss of light and improve the sensitivity of the sensor. The influences of the air hole diameter, the distance between D-shaped holes and core, and the counterclockwise rotation angle of D-shaped holes on sensing performance are studied. The simulation results show that the wavelength sensitivity of the designed sensor can be as high as 10100 nm/RIU and the resolution can reach 9.9 × 10 -6 RIU.

Short-duration chemoprophylaxis might reduce incidence of deep vein thrombosis in Asian patients undergoing total knee arthroplasty

Zhang Siyuan,Htet Kway Swar,Tan Xin Yang,Wang Xinyu,Wang Wilson,Chua Weiliang 대한슬관절학회 2020 대한슬관절학회지 Vol.32 No.-

Venous thromboembolism (VTE) is a serious complication that may occur after total knee arthroplasty (TKA), leading to the recommendation of routine chemoprophylaxis by international guidelines. This study aims to determine if short-duration chemoprophylaxis after TKA reduces the incidence of VTE in an Asian population.A retrospective study of 316 patients who underwent unilateral primary TKA between 1 January 2011 and 31 December 2013 was conducted. All patients received mechanical prophylaxis. One hundred seventeen patients (37%) received additional chemoprophylaxis, whereas 199 patients (63%) did not. A Doppler ultrasound (DUS) of both lower limbs was conducted for all patients within 6 days after surgery (median = 3 days) to assess for both proximal and distal DVT. Chemoprophylaxis in the form of enoxaparin (low molecular weight heparin; LMWH), aspirin, or heparin was administered until patients had a normal DUS, for a median duration of 4 days. Patients were followed up clinically for a minimum of 6 months to monitor for delayed or recurrent VTE and at least 2 years for patient-reported outcome measures. Overall, 24 patients (7.59%) developed deep vein thrombosis (DVT): three proximal and 21 distal DVTs. Twenty-three of the 24 patients were asymptomatic. Twenty of 199 patients (10.05%) with only mechanical prophylaxis developed DVT, whereas four of 117 patients (3.42%) with additional chemoprophylaxis developed DVT. Multivariate analysis showed that chemoprophylaxis use was associated with reduced incidence of DVT (odds ratio = 0.19, p value = 0.011). Other factors associated with increased DVT incidence include female gender (odds ratio = 5.45, p value = 0.034), positive history of cancer (odds ratio = 5.14, p value = 0.044), and increased length of stay in hospital (odds ratio = 1.19, p value < 0.001). Our study has shown that despite the low incidence of DVT in Asian patients undergoing TKA, short-duration chemoprophylaxis might be effective in reducing the incidence of DVT. However, most DVTs observed in our study were distal and may be of limited clinical significance. Further studies are needed to investigate the impact of chemoprophylaxis use on the incidence of PE and overall mortality rates among Asian patients.

Effects of inoculating different mycorrhizal fungi on rhizosphere soil fungi and nutrient uptake of blueberry

Song Zejun,Xiao Longhai,Guo Xiaolan,Zhu Yunzheng,An Xiaoli,Tan Yi,Zhang Xinyu,Wang Delu 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.1

The aim of this study was to explore the eff ects of inoculating with diff erent fungi on the structural characteristics of soil fungal communities and the nutrient absorption of blueberry plants. The fungi DSE ( Phialocephala fortinii ), SS ( Penicillium pinophilum ), ZB ( Cladosporium cladosporioides ), QMK ( Chaetomium globosum ), and LZ ( Schizophyllum commune ) were selected for this study. The soil fungal diversity and community structure in the blueberry rhizosphere were compared after inoculation with these diff erent mycorrhizal fungi. The fungal diversity in the blueberry rhizosphere soil was signifi cantly higher after inoculation with DSE, QMK, and ZB than after inoculation with SS and LZ. The dominant class, order, family, and genus of soil fungi were Eurotiomycetes (25.49–36.91%), Eurotiales (24.78–35.00%), Aspergillaceae (24.47–34.74%), and Penicllium (24.34–34.63%), respectively. Among all the fungal treatments, DSE led to the highest abundance of dominant classes, orders, families, and genera. Inoculation with SS, LZ, and DSE increased the contents of nitrogen, phosphorus, and potassium in blueberry plants. The uptake of nitrogen, phosphorus, and potassium were highest in DSE-inoculated plants. The nitrogen content was signifi cantly higher in ZB-inoculated plants than in the control. A TOPSIS analysis showed that among the fi ve fungi, DSE was the best strain to improve the diversity of the blueberry rhizosphere fungal community and promote nutrient uptake by blueberry plants, followed by LZ and ZB. These fi ndings provide scientifi c data for the develop ment and utilization of soil microbial resources, and provide useful information for improving the cultivation and production of blueberries.

Magnetic-field-tuned Insulator to Conductor Transition in Magnetorheological Suspension

Xiongbo Yang,Yuhuan Jiang,Yuehua Huang,Ruizhen Xu,Hongguang Piao,Gaomeng Jia,Xinyu Tan 한국자기학회 2014 Journal of Magnetics Vol.19 No.4

Magnetorheological suspensions (MRSs) are smart materials that have the potential to revolutionize several industrial sectors because of their special rheological behaviors. In this paper, MRS, based on carbonyl iron (CI) microparticles that were dispersed in silicone oil with oleic acid, were prepared. We showed that the electroconductibility of MRS was significantly influenced by the intensity of the external magnetic field that was applied. The resistance value can vary from infinite to below 300 Ω after applying an external magnetic field. The results indicated that this MRS had the property of magnetic-field-tuned insulator to conductor transition. This system has potential applications in controllable MRS electrical devices.

Regenerative Er-doped Fiber Amplifier System for High-repetition-rate Optical Pulses

Yan Liu,Kan Wu,Nanxi Li,Lanling Lan,Seongwoo Yoo,Xuan Wu,Perry Ping Shum,Shuguang Zeng,Xinyu Tan 한국광학회 2013 Current Optics and Photonics Vol.17 No.5

A regenerative Er-doped fiber amplifier system for a high-repetition-rate optical pulse train is investigated for the first time. A signal pulse train with a wavelength tuning range of 18 nm is produced by a passive mode-locked fiber laser based on a nonlinear polarization rotation technique. In order to realize the amplification, an optical delay-line is used to achieve time match between the pulses’ interval and the period of pulse running through the regenerative amplifier. The 16 dB gain is obtained for an input pulse train with a launching power of -30.4 dBm, a center wavelength of 1563.4 nm and a repetition rate of 15.3 MHz. The output properties of signal pulses with different center wavelengths are also discussed. The pulse amplification is found to be different from the regenerative amplification system for CW signals.