Cloning and expression of juvenile hormone esterase of the diamondback moth, Plutella xylostellla (Linn.)

Xiaojun Gu,Yonggyun Kim 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

Juvenile hormone is the most important hormone inside the insect, but its circulating titer should be under tight control. Then enzymes involved in JH metabolism, especially juvenile hormone esterases (JHEs) play critical roles in insect metamorphosis and reproduction. Within a set of 11 JHEs predicted in the diamondback moth (DBM) genome, Plutlla xylostella (Linn.) we identified one gene that contained the main functional motifs of insect JHEs. Its expression and transcript levels during egg, different instar of larvae, prepuae, pupae and adult stages were measured. Also, its expression in the epidermis, midgut and fatbody of the 4 th instar larvae was compared. The changes in enzyme activity in the 4th instar larvae were determined.

Impact of Size on Humidity Sensing Property of Copper Oxide Nanoparticles

Yang Gu,Huina Jiang,Zi Ye,Ning Sun,Xuliang Kuang,Weijing Liu,Gaofang Li,Xiaojun Song,Lei Zhang,Wei Bai,Xiaodong Tang 대한금속·재료학회 2020 ELECTRONIC MATERIALS LETTERS Vol.16 No.1

Three sizes of CuO nanosheets were synthesized by hydrothermal method. The structure and morphology of CuO nanosheets were characterized by X-ray difraction and scanning electron microscopy. Dielectrophoresis nano-manipulation technique was employed to arrange the materials on pre-designed Ti/Au electrodes to fabricate the three humidity sensors, and the sensing properties were then tested. The experimental results show that the sensitivity greatly increases with the decreasing size of CuO nanosheets, the sensitivity of sensor a, b, c are 369%, 3278%, 22,611% in 97.3% RH, respectively. The smaller sized CuO nanomaterials have better response characteristic, the response time of sensor a, b, c under 11.3–97.3% RH are 53 s, 49 s, 32 s, respectively. And correspondingly, hysteresis properties and the repeatability are also a little infuenced. In addition, based on complex impedance spectroscopy and multilayer adsorption theory, the impact of size on humidity sensing property was discussed. The results indicated the feasibility to obtain higher performance of humidity sensor, especially the higher sensitivity, via employment the smaller size sensing nanomaterials.

The Prognostic Significance of Notch1 and Fatty Acid Binding Protein 7 (FABP7) Expression in Resected Tracheobronchial Adenoid Cystic Carcinoma: A Multicenter Retrospective Study

Mian Xie,Xiaojun Wu,Jinjun Zhang,Chaosheng He,Shenhai Wei,Junyao Huang,Xinge Fu,Yingying Gu 대한암학회 2018 Cancer Research and Treatment Vol.50 No.4

Purpose Adenoid cystic carcinoma (ACC) of the trachea and bronchus is a rare tumor. Although MYBNFIB oncogene fusion and Notch1 mutation have been identified in ACC, little is known about the expression and clinical significance of Notch1 and its target gene fatty acid binding protein 7 (FABP7) in tracheobronchial ACC. Materials and Methods Primary tracheobronchial ACC that were resected between 1998 and 2014 were identified through the pathology and oncology database from five thoracic oncology centers in China. A tissue array was constructed from the patients’ samples and the expressions of Notch1 and FABP7 were evaluated by immunohistochemistry. The association between the expression of both markers and survival was determined. Results Overexpression of Notch1 and FABP7, detected in 37.8% and 38.3% of 368 patients with tracheobronchial ACC, respectively, was an independent prognostic indicator for recurrencefree survival (RFS) by multivariable Cox proportional hazard model (p=0.032 and p=0.048, respectively). Overexpression of Notch1, but not of FABP7, predicted overall survival (OS) (p=0.018). When categorized into four groups according to coexpression of Notch1 and FABP7, patients with overexpression of both Notch1 and FABP7 belonged to the group with the shortest RFS and OS (p=0.01 and p=0.048, respectively). Conclusion Expression of Notch1 and FABP7, and coexpression of Notch1 and FABP7, is strongly associated with poor survival in resected tracheobronchial ACC. These data are consistent with the hypothesis that poor differentiation of tracheobronchial ACC correlates with the activation of Notch signaling.

Polishing surface integrity of TC17 aeroengine blades

De Liu,Yaoyao Shi,Xiaojun Lin,Chao Xian,Zhiyang Gu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.2

Aeroengine blades have high requirements for surface integrity. Through the flexible polishing of TC17 engine blades, the surface integrity of polished blades is improved. First, the influence of process parameters on polishing force and polishing temperature is analyzed through experiments. The influence of parameters on surface roughness is analyzed, and the relationship between wear debris shape and surface morphology under different process parameters are analyzed from the microscopic level. Second, the influence of process parameters on the residual stress of the polished surface is analyzed in accordance with the polishing force and polishing temperature. Then, the variation of the residual stress of the blade after milling and polishing is analyzed. In addition, the microstructure of the metamorphic layer after milling and polishing is analyzed. Experimental results show that reasonable polishing parameters can effectively improve the surface integrity of the blade.

Conservative therapy with metformin plus megestrol acetate for endometrial atypical hyperplasia

Weiwei Shan,Chao Wang,Zhenbo Zhang,Chao Gu,Chengcheng Ning,Xuezhen Luo,Qiongjie Zhou,Xiaojun Chen 대한부인종양학회 2014 Journal of Gynecologic Oncology Vol.25 No.3

Objective: To compare the efficacy of metformin plus megestrol acetate (MA) with that of MA alone for treating endometrial atypical hyperplasia (EAH). Methods: This pilot study included 16 EAH patients who met at least one metabolic syndrome (MS) criterion and received either adjunctive metformin plus MA (MET group) or MA monotherapy (MA group). Each patient in the MA group received 160 mg of MA daily, whereas patients in the MET group received the same dose of MA plus 0.5 g of metformin thrice daily. Treatment response was assessed by histological examination of dilation and curettage specimens obtained after 12 weeks of therapy. Results: Each group had eight patients, and half of the patients in each group were diagnosed with MS. The complete response (CR) rate was 75% (6/8) in the MET group and 25% (2/8) in the MA group (p=0.105). Complications of MS did not affect the response rates in either group. In the MET group, 75% (3/4) of the patients had CR in the presence or absence of MS. In the MA group, 50% (2/4) of the patients with MS had CR, whereas no patient without MS had CR. No irreversible toxicities were observed. Conclusion: Metformin plus MA may be a potential alternative therapy for treating EAH, and the MS status of patients may have no effect on the efficacy of metformin plus MA therapy.

Variation of sexual dimorphism and asymmetry in disease expression of inflammatory arthritis among laboratory mouse models with different genomic backgrounds

Wei Dong,Cheng Tian,Z. Galvin Li,David Brand,Yanhong Cao,Xiaoyun Liu,Jiamin Ma,Andy Chai,LindaK.myers,Jian Yan,Karen Hasty,John Stuart,Yan Jiao,Weikuan Gu,Xiaojun Cai 한국실험동물학회 2023 Laboratory Animal Research Vol.39 No.4

Sex difference has shown in the arthritis diseases in human population and animal models. We investigate how the sex and symmetry vary among mouse models with different genomic backgrounds. Disease data of sex and limbs accumulated in the past more than two decades from four unique populations of murine arthritis models were analyzed. They are (1) interleukin-1 receptor antagonist (IL-1ra) deficient mice under Balb/c background (Balb/c KO); (2) Mice with collagen II induced arthritis under DBA/1 background; (3) Mice with collagen II induced arthritis under C57BL/6 (B6) background and (4) A F2 generation population created by Balb/c KO X DBA/1 KO. Our data shows that there is a great variation in sexual dimorphism for arthritis incidence and severity of arthritis in mice harboring specific genetic modifications. For a F2 population, the incidence of arthritis was 57.1% in female mice and 75.6% in male mice. There was a difference in severity related to sex in two populations: B6.DR1/ B6.DR4 (P < 0.001) and F2 (P = 0.023) There was no difference Balb/c parental strain or in collagen-induced arthritis (CIA) in DBA/1 mice. Among these populations, the right hindlimbs are significantly higher than the scores for the left hindlimbs in males (P < 0.05). However, when examining disease expression using the collagen induced arthritis model with DBA/1 mice, sex-dimorphism did not reach statistical significance, while left hindlimbs showed a tendency toward greater disease expression over the right. Sexual dimorphism in disease expression in mouse models is strain and genomic background dependent. It sets an alarm that potential variation in sexual dimorphism among different racial and ethnic groups in human populations may exist. It is important to not only include both sexes and but also pay attention to possible variations caused by disease expression and response to treatment in all the studies of arthritis in animal models and human populations.