Species Classification and Quality Assessment of Chaihu (Radix Bupleuri) Based on High-Performance Liquid Chromatographic Fingerprint and Combined Chemometrics Methods

Xiao-Jie Li,Jie Hu,Zhen-Yu Li,Xue-Mei Qin,Li-Zeng Zhang,Xiao-Qing Guo 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.6

A high-performance liquid chromatographic (HPLC) method was established to analyze 36 Chaihu (Radix Bupleuri) samples collected from three species (Bupleurum chinense DC., B. scorzonerifolium Willd. and B. smithii Wolff.). Addition of trifluoroacetic acid into the mobile phase resulted in fingerprint chromatograms with stable baselines. There were thirty-two characteristic peaks in the standard fingerprint of B. chinense DC. Different recognition pattern methods, including similarity analysis (SA), hierarchical cluster analysis (HCA), principal component analysis (PCA) and partial least squares-discrimination analysis (PLS-DA) were utilized to analyze the 36 samples based on the contents of chemical constituents. Consistent results from SA, HCA and PCA analysis illustrated the rationalisation for why B. smithii Wolff. was not quoted in the Chinese Pharmacopoeia and classified samples were in agreement with their species. PLS-DA loading plots showed the chemical markers which had the most influences on the separation among different species. However, SA, HCA and PCA could not differentiate between wild and cultivated B. chinense DC. as well as between samples from different provinces. HPLC fingerprint in combination with chemometric techniques provided a very flexible and reliable method for homogeneity evaluation and quality assessment of traditional Chinese medicine.

Bismuth-Doped Phosphate Glasses and H+-Implanted Waveguides

Jie Zhang,Jing-Yi Chen,Chun-Xiao Liu,Liao-Lin Zhang,She-Bao Lin 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.76 No.6

The work reports on the preparation and optical characterization of 75P2O5-15B2O3-9Al2O3-Bi2O3 glass by using the melt-quenching method and absorption spectroscopy, respectively. Further, 0.4-MeV proton implantation of a fluence of 8.0 × 10^{16} ions/cm^2 was carried out on this glassy sample and it was tested as a waveguide at a wavelength of 632.8 nm for the first time to our knowledge. The m-line curves of the waveguide were measured before and after successive annealing treatments. The formation theory of the planar waveguides is discussed through simulations of the energy loss distribution and analysis of the reconstructed refractive index profile. The results should be useful in the design and fabrication of bismuth-doped phosphate glass and its use in integrated photonics devices.

Pd Nanoparticles Self-Assembled on Fluorine-Modified MWCNTs as Electro-Catalysts for Methanol Electro-Oxidation

Xiao-Feng Zhang,Peng Dong,Ying-Jie Zhang,Xi-Kun Yang,Shu-Biao Xia,Zhen-Hua Jin,Ming-Li Xu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.3

Multi-walled carbon nanotubes (MWCNTs) were modified by hydrogen fluoride (HF) in a simple method. With the help of fluorine, Pd nanoparticles (3.9 nm) synthesized by a one-step photochemical reduction were uniformly self-assembled on the active sites of functionalized MWCNTs and a new catalyst (Pd/HF-MWCNT) was obtained. UV–Vis absorption spectra, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) were used. The results demonstrated that –F groups were introduced onto the surface of MWCNTs and C–F chemical bonds were formed. In addition, the electronic structure of Pd was changed. Pd–F coordination bond maybe formed between F atom and Pd atom. Cyclic voltammetry and chronoamperometry tests indicated that electrocatalytic activity of Pd/HF-MWCNTs catalyst for methanol in alkaline medium was about 1.6 times higher than that of the commercial Pd/C (JM) catalyst at the same condition. This new functionalized method has the advantages of simple step and safe operation. It is very significant to improve the wide application of MWCNTs and the commercial development of direct methanol fuel cells (DMFCs).

No-binding Molding Technology Development for Waste Rubber Recycling

( Xiao Jie Zhang ),( Sung Woo Hong ),( Jin Kuk Kim ) 한국고무학회 2018 엘라스토머 및 콤포지트 Vol.53 No.1

In this study, a new process was developed to develop the waste rubber material recycling system. Firstly, the blending of the reclaimed rubber/virgin rubber as a compounding technology was investigated in this study. Secondly, the removal of odor by using zeolite. Thirdly, the continuous crosslinking process technology was studied the technology can solve the environmentally harmful substances and economic problems. Based on this technology, we have started to develop application technologies such as floor mats and rubber sheets for forklifts, and will further study the environmentally conscious products in various ways. Our research will contribute to the recycling industry.

Anomalous magnetization jumps in granular Pb superconducting films

Zhang An-Lei,Jiang Wan-Yan,Chen Xing-Hong,Zhang Xiao-Ke,Lu Wen-Lai,Chen Fei,Feng Zhen-Jie,Cao Shi-Xun,Zhang Jin-Cang,Ge Jun-Yi 한국물리학회 2022 Current Applied Physics Vol.35 No.-

In granular superconductors, the grain boundaries are closely related to the vortex dynamics and the macroscopic superconducting properties. In our research, Pb films with different grain sizes were prepared by tuning the substrate temperature. With the grain size decreasing, Pb films are prone to feature the anomalous magnetization jumps in the M − T curves, while in the M − H curves flux avalanche happens. Both phenomena appear in the same region of the H − T phase diagram and thus are considered to have the same origin. The further theoretical analysis shows that with grain size decreasing the pinning mechanism evolves from a mixed δTc and δl pinning to the δl pinning mechanism. The results shed light on the study of pinning mechanism for granular superconductors and is beneficial to the potential application of manipulating vortex pinning by regulation of intrinsic defects.

Identification and Functional Analysis of the Chain Length Determinant Gene ste8 Involved in the Biosynthesis of Ebosin by Streptomyces sp.139

( Zhang Yang ),( Xiao Hua Li ),( Xiao Qaing Qi ),( Jun Jie Shan ),( Rong Jiang ),( Lian Hong Guo ),( Ren Zhang ),( Yuan Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.11

Ebosin, a novel exopolysaccharide produced by Streptomyces sp. 139, has obvious antirheumatic arthritis activity in vivo, and its biosynthesis gene cluster (ste), consisting of 27 open reading frames, has been identified. This paper reports our study of the gene functionality of ste8, the predicted protein product of which is homologous to some bacterial chain length determinant Wzz proteins. For characterization of Ste8, ste8 was cloned and expressed in the mutant strain E. coli 086:H2 (Δwzz). The functional complementation of wzz by ste8 was demonstrated by the restoration of wild-type lipopolysaccharide biosynthesis and increased levels of serum resistance of E. coli 086:H2 (Δwzz) (pET30a-ste8). To examine the function of ste8 in ebosin biosynthesis, the gene was knocked out with a double crossover via homologous recombination. The molecular weight of the ebosin derivative EPS-8m produced by the mutant Streptomyces sp. 139 (ste8-) was much lower than that of ebosin, and the binding activity of EPS-8m for IL-1R decreased significantly compared with ebosin. These results demonstrate that ste8 encodes a chain length determinant (Wzz) that functions in ebosin biosynthesis.

Effects of IBA on rooting ability of Cinnamomum bodinieri citral type micro-shoots from transcriptomics analysis

Xiao Zufei,Jin Zhinong,Zhang Beihong,Li Feng,Yu Faxin,Zhang Haiyan,Lü Xiongwei,Zhang Jie 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.4

Cinnamomum bodinieri is a useful plant that could be improved by increasing its cultivation rate. To explore the optimal concentration of exogenously applied IBA for root formation and development in the C. bodinieri citral type, micro-shoots and the fresh tissues of their bases were subjected to morphological and transcriptome analyses, respectively. When plantedQuery on a rooting medium (1/2 MS) with 1.5 mg/L IBA for 25 days, the rooting rates and root numbers were significantly higher than those obtained with 0, 0.5, 1.0, or 2.0 mg/L IBA treatment. Transcriptome analyses conducted 0 day, 7 days, 11 days, and 14 days after planting in the optimal rooting medium demonstrated that the IBA modified the plant’s carbohydrate metabolism and auxin signaling pathway. The root-specific expressions of TIR1, GH3, and SAUR were up-expressed while AUX/IAA was repressed, which contributed to the formation of lateral root initiation sites and root cell elongation. At the same time, d-glucose synthesis increased and the accumulation of starch decreased as more carbohydrates were used to form and develop roots instead of being used for the rapid cell division required for shoot growth. This study identified the optimal rooting medium for the C. bodinieri citral type and determined the molecular mechanisms involved in the regulation of exogenously applied IBA during root formation and development at the transcriptional level.

Elevated FBXL6 expression in hepatocytes activates VRK2-transketolase-ROS-mTOR-mediated immune evasion and liver cancer metastasis in mice

Zhang Jie,Lin Xiao-Tong,Yu Hong-Qiang,Fang Lei,Wu Di,Luo Yuan-Deng,Zhang Yu-Jun,Xie Chuan-Ming 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Metastatic hepatocellular carcinoma (HCC) is the most lethal malignancy and lacks effective treatment. FBXL6 is overexpressed in human hepatocellular carcinoma (HCC), but whether this change drives liver tumorigenesis and lung metastasis in vivo remains unknown. In this study, we aimed to identify FBXL6 (F-Box and Leucine Rich Repeat Protein 6) as a key driver of HCC metastasis and to provide a new paradigm for HCC therapy. We found that elevated FBXL6 expression in hepatocytes drove HCC lung metastasis and was a much stronger driver than Kras mutation (KrasG12D/+;Alb-Cre), p53 haploinsufficiency (p53+/-) or Tsc1 loss (Tsc1fl/fl;Alb-Cre). Mechanistically, VRK2 promoted Thr287 phosphorylation of TKT and then recruited FBXL6 to promote TKT ubiquitination and activation. Activated TKT further increased PD-L1 and VRK2 expression via the ROS-mTOR axis, leading to immune evasion and HCC metastasis. Targeting or knockdown of TKT significantly blocked FBXL6-driven immune evasion and HCC metastasis in vitro and in vivo. Notably, the level of active TKT (p-Thr287 TKT) was increased and was positively correlated with the FBXL6 and VRK2 expression levels in HCC patients. Our work provides novel mechanistic insights into FBXL6-driven HCC metastasis and suggests that targeting the TKT-ROS-mTOR-PD-L1/VRK2 axis is a new paradigm for treating patients with metastatic HCC with high FBXL6 expression.

Patterns of Lymph Node Recurrence after Radical Surgery Impacting on Survival of Patients with pT1-3N0M0 Thoracic Esophageal Squamous Cell Carcinoma

Xiao-li Chen,,Tian-wu Chen,Zhi-jia Fang,Xiao-ming Zhang,Zhen-lin Li,Hang Li,Hong-jie Tang,Li Zhou,Dan Wang,Zishu Zhang 대한의학회 2014 Journal of Korean medical science Vol.29 No.2

The aim of this study was to investigate how patterns of lymph nodes recurrence after radical surgery impact on survival of patients with pT1-3N0M0 thoracic esophageal squamous cell carcinoma. One hundred eighty consecutive patients with thoracic esophageal squamous cell carcinoma underwent radical surgery, and the tumors were staged as pT1-3N0M0 by postoperative pathology. Lymph nodes recurrence was detected with computed tomography 3-120 months after the treatment. The patterns of lymph nodes recurrence including stations, fields and locations of recurrent lymph nodes, and impacts on patterns of survival were statistically analyzed. There was a decreasing trend of overall survival with increasing stations or fields of postoperative lymph nodes involved (all P<0.05). Univariate analysis showed that stations or fields of lymph nodes recurrence, and abdominal or cervical lymph nodes involved were prognostic factors for survival(all P<0.05). Cox analyses revealed that the field was an independent factor (P<0.05, odds ratio=2.73). Lymph nodes involved occurred predominantly in cervix and upper mediastinum (P<0.05). In conclusion, patterns of lymph node recurrence especially the fields of lymph nodes involved are significant prognostic factors for survival of patients with pT1-3N0M0 thoracic esophageal squamous cell carcinoma.

Fairness versus Efficiency in Chinese Environmental Policy: A Perspective from Environmental Policy Tools

( Xiao Jie Zhang ),( Ping Sun ) 한국정책학회 2011 International Journal of Policy Studies Vol.2 No.1

How to deal with the relationship between fairness and efficiency has been an important dispute in public policy practices, as is the case for environmental policy in Mainland China. This paper examines the fairness and efficiency principles in Chinese environmental policy from the perspective of environmental policy instruments. First, an evaluation framework for fairness and efficiency are established. Then, the authors evaluate different levels of fairness and efficiency principles inherent in various environmental policy tools based on the evaluation framework, after a brief investigation into the development of the policy instruments. The authors finally conclude that the notions in Chinese environmental policies have gradually transferred to the comprehensive efficiency principles that consist of political feasibility, economic costs, administrative operability and environmental effectiveness. The efficiency-oriented notion is beneficial to Chinese environmental protection enterprises, as it can achieve the environmental objectives with minimum political, administrative and economic costs.