Expression of Connexin 43 and E-cadherin Protein and mRNA in Non-small Cell Lung Cancers in Chinese Patients

Zhao, Jun-Qiang,Sun, Fang-Jie,Liu, Shan-Shan,Yang, Jun,Wu, Yu-Quan,Li, Gui-Shan,Chen, Qing-Yong,Wang, Jia-Xiang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Aim: Connexin 43 (Cx43) and E-cadherin are important biomarkers related with cancer. Their expression at protein and mRNA levels was here investigated in 50 primary lung carcinoma tissues and 20 samples of adjacent normal tissue of Chinese patients with non-small cell lung cancer (NSCLC). Methods: Protein and mRNA expression were evaluated by ABC immunohistochemistry and RT-PCR. Results: (1) The positive expression rates of Cx43 and E-cadherin protein were higher in the adjacent normal tissues than those in the primary lung carcinoma tissues; (2) the positive expression rates of Cx43 and E-cadherin protein decreased with NSCLC progression; (3) the expression of E-cadherin protein was not related with the pathological type of NSCLC; and (4) the relative quantity of the Cx43 or E-cadherin mRNA expression was correlated with the the histological type, clinical stage, cancer cell differentiation and the lymph node metastasis. Conclusion: The data suggested that the Cx43 and E-cadherin are reduced with NSCLC progression, and might be important biomarkers for judging the metastasis and prognosis.

Analysis of non-point and point source pollution load in DongPieHong Ditch

Shan, Yu,Chen, Jun,Jin, Jie,Song, YongLian,Liu, Jun,Wu, DongBiao,Wu, Ke Urban Science Institute 2019 도시과학 Vol.8 No.2

In this study, the DongPieHong Ditch were taken as the research object, five sampling points were set to measure the COD, NH₃-N,TNandTPindexes. The correlation and principal component analysis were used to judge the main pollution sources and calculate pollution contribution rate. According to the population in the basin, the load of point source pollution into the river was estimated. As a result, the load of COD, NH₃-N and TP into the river was 323.04t/a, 43.8t/a and 3.9t/a, respectively. According to the statistics of the rainfall in the basin, the concentrations of COD, TP and NH₃-N in the initial rainwater were measured and calculated for non-point source pollution, and the results shown that the inflow loads of COD, NH₃-N and TP into the river were 34.59t/a, 0.12t/a and 0.71t/a, respectively. It was found that the main cause of the pollution in the east flash flood gully was point source pollution, and the proportions of COD, NH₃-N and TP into the river were 90.33%, 99.72% and 84.61%, respectively.

Differences in the mandibular premolar positions in Angle Class I subjects with different vertical facial types

Jun Duan,Feng Deng,Wan-Shan Li,Xue-Lei Li,Lei-Lei Zheng,Gui-Yuan Li,Yan-Jie Bai 대한치과교정학회 2015 대한치과교정학회지 Vol.45 No.4

Objective: To compare the positions of the mandibular premolars in Angle Class I subjects according to vertical facial type. The results will provide a theoretical basis for predicting effective tooth movement in orthodontic treatment. Methods: Cephalometric parameters were determined using cone-beam computed tomography in 120 Angle Class I subjects. Subjects were categorized as short, normal, and long face types according to the Frankfort mandibular angle. Parameters indicating the position of the mandibular right premolars and the mandible were also measured. Results: The angle between the mandibular first premolar axis and buccal cortex, the distance between the root apex and buccal cortex, angle of vestibularization, arc of vestibularization, and root apex maximum movable distance were significantly greater in the short face type than in the long and norm face types. The angle between the mandibular second premolar axis and buccal cortex, the distance from root apex to buccal cortex, and the arc of vestibularization were significantly greater in the short face type than in the normal face type. Conclusions: There are significant differences in the mandibular premolar positions in Class I subjects according to vertical facial type.

Neuron Discharge and c-Fos Expression in the Nucleus of the Solitary Tract Following Electroacupuncture at Acupoints of the Yangming Stomach Meridian of Foot

Jun-Feng He,Jie Yan,Jiang-Shan Li,Jian-Hua Liu,Chao Wang,Xiao-Rong Chang,Ya-Ting Qu 사단법인약침학회 2013 Journal of Acupuncture & Meridian Studies Vol.6 No.2

The nucleus of the solitary tract (nucleus tractus solitarii; NTS) is a primary center for both visceral afferents and somatic afferents. Previous experiments have demonstrated that the NTS is closely connected to the stomach and acupoints in the Yangming Stomach Meridian of Foot (ST Meridian). In this study, extracellular recording and immunochemistry methods were used to analyze the discharge of neurons and c-Fos protein expression in the NTS following acupuncture at different acupoints and a nonacupoint. A total of 104 discharging neurons were detected in the NTS of 52 rats, of which 86 provided complete data. After acupuncture at Sibai (ST 2), Zusanli (ST 36), Neiting (ST 44), Quanliao (SI 18), and the nonacupoint, the neuron response rate in the NTS was 65.12%, 51.16%, 46.51%, 34.88% and 31.40% respectively. For neuron response rate, there was a significant difference among Sibai (ST 2), Zusanli (ST 36), Neiting (ST 44), Quanliao (SI 18), and the nonacupoint (p < 0.01 or p < 0.05). In the other 48 rats, the number of c-Fos immunoreactive neurons in the NTS by electroacupuncture (EA) at Sibai (ST 2) group was significantly higher than that EA at other acupoints and the nonacupoint (p < 0.05 or p < 0.01). EA at both Zusanli (ST 36) and Neiting (ST 44) increased c-Fos immunoreactive neurons significantly over EA at Quanliao (SI 18) and the nonacupoint (p < 0.05 or p < 0.01), while there was no difference between EA at Quanliao (SI 18) and the nonacupoint group (p > 0.05). The experiments demonstrated that the afferent convergence in NTS are different by body surface points stimulus, which suggests that the NTS might be a primary center in the central nervous system receiving acupoints stimulus from the ST Meridian.

Synthesis of pore-expanded mesoporous materials using waste quartz sand and the adsorption effects of methylene blue

Jun-Ming Hong,Bing Lin,Jie-Shan Jiang,Bor-Yann Chen,Chang-Tang Chang 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

Mesoporous material Q-MCM and EX-Q-MCM were synthesized using waste quartz (99.9% SiO2) sand as a silicon source via the sol–gel method. The silicon source was extracted from the waste quartz sand using the hydrothermal method. This study selected methylene blue as a target dye to investigate the dye adsorption performance onto the EX-Q-MCM. The effects on adsorption performance under different initial dye concentrations and different pH values were also studied. The results show that the maximum adsorption capacity was 96.9 mg g 1 and a high pH is favorable for adsorption. Further, equilibrium isotherms of the adsorption of methylene blue on EX-Q-MCM are well described by the Freundlich model. It reveals that the adsorption of methylene blue onto the Q-MCM is a multilayer adsorption.

Parkinson’s Disease with Fatigue: Clinical Characteristics and Potential Mechanisms Relevant to α-Synuclein Oligomer

Li-Jun Zuo,Shu-Yang Yu,Fang Wang,Yanghui Xia,Ying-Shan Piao,Yang Du,Teng-Hong Lian,Rui-Dan Wang,Qiu-Jin Yu,Ya-Jie Wang,Xiao-Min Wang,Piu Chan,Sheng-Di Chen,Yongjun Wang,Wei Zhang 대한신경과학회 2016 Journal of Clinical Neurology Vol.12 No.2

Background and Purpose The aim of this study was to identify the clinical characteristics and potential mechanisms relevant to pathological proteins in Parkinson’s disease (PD) patients who experience fatigue. Methods PD patients (n=102) were evaluated using a fatigue severity scale and scales for motor and nonmotor symptoms. The levels of three pathological proteins—α-synuclein oligomer, β-amyloid (Aβ)1-42, and tau—were measured in 102 cerebrospinal fluid (CSF) samples from these PD patients. Linear regression analyses were performed between fatigue score and the CSF levels of the above-listed pathological proteins in PD patients. Results The frequency of fatigue in the PD patients was 62.75%. The fatigue group had worse motor symptoms and anxiety, depression, and autonomic dysfunction. The CSF level of α-synuclein oligomer was higher and that of Aβ1-42 was lower in the fatigue group than in the non-fatigue group. In multiple linear regression analyses, fatigue severity was significantly and positively correlated with the α-synuclein oligomer level in the CSF of PD patients, after adjusting for confounders. Conclusions PD patients experience a high frequency of fatigue. PD patients with fatigue have worse motor and part nonmotor symptoms. Fatigue in PD patients is associated with an increased α-synuclein oligomer level in the CSF

Microstructure Distribution and Grain Coarsening Model of GCr15 Steel in the Laser Surface Treatment

Jin‑shan Chen,Zhen‑xing Li,Ya‑jie Chu,Jie Chen, Xin‑jun Shen 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.10

The GCr15 steel was subjected to laser surface treatment using a diode laser with rectangular spot. Based on the simulatedtemperature field, the microstructure distribution of the laser hardened layer was analyzed, and an empirical equation wasproposed to predict the peak temperature at different depths. The results indicated that with the decrease of depth, the peaktemperature increased exponentially, and the peak temperature at different depths was approximately linear with the powerdensity and the reciprocal of square root of scanning speed. High peak temperature led to the coarsening of grain and thesignificant dissolution of cementite near the surface of hardened layer. At the deeper position of hardened layer, the prioraustenite grain size decreased slightly due to high nucleation rate. The cementite dissolution and coarsening occurred simultaneouslynear the surface, and the cementite dissolution process was postponed due to the significant enrichment of Cr nearthe interface of austenite and cementite. Additionally, an empirical model about grain coarsening was established, which canbe used to predict the prior austenite grain size near the surface during the laser surface treatment.

Association of XRCC3 Thr241Met Polymorphisms and Gliomas Risk: Evidence from a Meta-analysis

Liang, Hong-Jie,Yan, Yu-Lan,Liu, Zhi-Ming,Chen, Xu,Peng, Qi-Liu,Wang, Jian,Mo, Cui-Ju,Sui, Jing-Zhe,Wu, Jun-Rong,Zhai, Li-Min,Yang, Shi,Li, Tai-Jie,Li, Ruo-Lin,Li, Shan,Qin, Xue Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

The relationship between the X-ray repair cross-complementing group 3 (XRCC3) Thr241Met polymorphism and gliomas remains inclusive or controversial. For better understanding of the effect of XRCC3 Thr241Met polymorphism on glioma risk, a meta-analysis was performed. All eligible studies were identified through a search of PubMed, Elsevier Science Direct, Excerpta Medica Database (Embase) and Chinese Biomedical Literature Database (CBM) before May 2013. The association between the XRCC3 Thr241Met polymorphism and gliomas risk was conducted by odds ratios (ORs) and 95% confidence intervals (95% CIs). A total of nine case-control studies including 3,533 cases and 4,696 controls were eventually collected. Overall, we found that XRCC3 Thr241Met polymorphism was significantly associated with the risk of gliomas (T vs. C: OR=1.10, 95%CI=1.01-1.20, P=0.034; TT vs. CC: OR=1.30, 95%CI=1.03-1.65, P=0.027; TT vs. TC/CC: OR=1.29, 95%CI=1.01-1.64, P=0.039). In the subgroup analysis based on ethnicity, the significant association was found in Asian under four models (T vs. C: OR=1.17, 95%CI=1.07-1.28, P=0.00; TT vs. CC: OR=1.79, 95%CI=1.36-2.36, P=0.00; TT vs. TC/CC: OR=1.75, 95%CI=1.32-2.32, P=0.00; TT/TC vs. CC: OR=1.11,95% CI=1.02-1.20). This meta-analysis suggested that the XRCC3 Thr241Met polymorphism is a risk factor for gliomas, especially for Asians. Considering the limited sample size and ethnicities included in the meta-analysis, further large scale and well-designed studies are needed to confirm our results.

Performance of Fixing Agents in Controlling Micro-Stickies in Recycled Newsprint Pulp

WANG LI-JUN,CHEN FU-SHAN,ZHOU LIN-JIE 한국펄프·종이공학회 2006 한국펄프종이학회 기타 간행물 Vol.- No.-

The microstickes control effects of some fixing agents, including an inorganic PAC, an organic polyamine (PA) and polydiallydimethyl ammonium chloride (Pdadmac), and a high cationic starch (HCS), were investigated, together with their effects on wet end performances and physical properties of handsheets. Despite that the HCS and Pdadmac had lower cationic charge densities than the PA and PAC (the HCS being even lower), they gave higher zeta potentials to fibers, and lower turbidities, cationic demands and residual COD contents to the pulp liquid phases than the PA and PAC did. In all cases, the HCS showed even better effects than the Pdadmac. In addition, drainage speed was also much higher by the HCS treatments although paper formation was worsened. All the phenomena showed that the HCS can fix more dissolved and colloidal substances to cellulose fibers, indicating that the HCS functioned mainly with flocculation and even hydrogen bonding mechanisms. Data on optical properties further indicated that the HCS interacted preferentially with colloidal substances, since it fixed more “dirty” microstickes to fibers which decreased more sheet brightness while increasing more sheet opacity (with both higher light absorption and scattering coefficients). Interestingly, the organic fixing agents did not decrease tensile, tearing, and folding strengths of paper sheets made from 100% recycled newsprint pulp, except when they were dosed in high amounts. On the contrary, the inorganic PAC had more serious negative effects on the strength properties, especially on folding endurance. The study suggested that proper use of the HCS can lead to better microstickies control effects than traditional agents and methods.

Coronary Intravascular Lithotripsy Versus Rotational Atherectomy in an Asian Population: Clinical Outcomes in Real-World Patients

Khung Keong Yeo,Jie Jun Wong,Sridharan Umapathy,Yann Shan Keh,Yee How Lau,Jonathan Yap,Muhammad Idu,Chee Yang Chin,Jiang Ming Fam,Boon Wah Liew,Chee Tang Chin,Philip En Hou Wong,Tian Hai Koh 대한심장학회 2022 Korean Circulation Journal Vol.52 No.4

Background and Objectives: We compared real-world clinical outcomes of patients receiving intravascular lithotripsy (IVL) versus rotational atherectomy (RA) for heavily calcified coronary lesions. Methods: Fifty-three patients who received IVL from January 2017 to July 2020 were retrospectively compared to 271 patients who received RA from January 2017 to December 2018. Primary endpoints were in-hospital and 30-day major adverse cardiovascular events (MACE). Results: IVL patients had a higher prevalence of acute coronary syndrome (56.6% vs 24.4, p<0.001), multivessel disease (96.2% vs 73.3%, p<0.001) and emergency procedures (17.0% vs 2.2%, p<0.001) compared to RA. In-hospital MACE (11.3% vs 5.9%, p=0.152), MI (7.5% vs 3.3%, p=0.152), and mortality (5.7% vs 3.0%, p=0.319) were not statistically significant. 30-day MACE was higher in the IVL cohort vs RA (17.0% vs 7.4%, p=0.035). Propensity score adjusted regression using IVL was also performed on in-hospital MACE (odds ratio [OR], 1.677; 95% confidence interval [CI], 0.588–4.779) and 30-day MACE (OR, 1.910; 95% CI, 0.774–4.718). Conclusions: These findings represent our initial IVL experience in a high-risk, real-world cohort. Although the event rate in the IVL arm was numerically higher compared to RA, the small numbers and retrospective nature of this study preclude definitive conclusions. These clinical outcomes are likely to improve with greater experience and better case selection, allowing IVL to effectively treat complex calcified coronary lesions.