미육군 교육용 AA온라인게임 품질모형에 의한 버그시험 평가

노성운,최성 한국정보처리학회 2005 정보처리학회지 Vol.12 No.2

Arthrobacter soli sp. nov., a Novel Bacterium Isolated from Wastewater Reservoir Sediment

노성운,Youlboong Sung,Young-Do Nam,Ho-Won Chang,김경호,윤정훈,전체옥,Jin-Woo Bae,오희목,Jin-Woo Bae 한국미생물학회 2008 The journal of microbiology Vol.46 No.1

A novel Gram-positive bacterium, designated SYB2^T, was isolated from wastewater reservoir sediment, and a polyphasic taxonomic study was conducted based on its morphological, physiological, and biochemical features, as well as the analysis of its 16S rRNA gene sequence. During the phylogenetic analysis of the strain SYB2^T, results of a 16S rRNA gene sequence analysis placed this bacterium in the genus Arthrobacter within the family Micrococcaceae. SYB2T and Arthrobacter protophormiae ATCC 19271^T, the most closely related species, both exhibited a 16S rRNA gene sequence similarity of 98.99%. The genomic DNA G+C content of the novel strain was found to be 62.0 mol%. The predominant fatty acid composition was anteiso-C15:0, anteiso-C_17:0, iso-C_16:0, and iso-C_15:0. Analysis of 16S rRNA gene sequences and DNA-DNA relatedness, as well as physiological and biochemical tests, showed genotypic and phenotypic differences between strain SYB2^T and other Arthrobacter species. The type strain of the novel species was identified as SYB2^T (= KCTC 19291T= DSM 19449T). A novel Gram-positive bacterium, designated SYB2^T, was isolated from wastewater reservoir sediment, and a polyphasic taxonomic study was conducted based on its morphological, physiological, and biochemical features, as well as the analysis of its 16S rRNA gene sequence. During the phylogenetic analysis of the strain SYB2^T, results of a 16S rRNA gene sequence analysis placed this bacterium in the genus Arthrobacter within the family Micrococcaceae. SYB2T and Arthrobacter protophormiae ATCC 19271^T, the most closely related species, both exhibited a 16S rRNA gene sequence similarity of 98.99%. The genomic DNA G+C content of the novel strain was found to be 62.0 mol%. The predominant fatty acid composition was anteiso-C15:0, anteiso-C_17:0, iso-C_16:0, and iso-C_15:0. Analysis of 16S rRNA gene sequences and DNA-DNA relatedness, as well as physiological and biochemical tests, showed genotypic and phenotypic differences between strain SYB2^T and other Arthrobacter species. The type strain of the novel species was identified as SYB2^T (= KCTC 19291T= DSM 19449T).

Rhodopirellula rosea sp. nov., a Novel Bacterium Isolated from an Ark Clam Scapharca broughtonii

노성운,이해원,Kyung June Yim,신나리,이지나,원태웅,Na-Lae Lim,김대경,배진우 한국미생물학회 2013 The journal of microbiology Vol.51 No.3

A novel Gram-negative, motile, and ovoid-shaped strain, LHWP3T, which belonged to the family Planctomycetaceae in the phylum Planctomycetes, was isolated from a dead ark clam Scapharca broughtonii collected during a mass mortality event on the south coast of Korea. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the isolate was most closely related to the type strain of Rhodopirellula baltica, with a shared 16S rRNA gene sequence similarity of 94.8%. The isolate grew optimally at 30°C in 4–6% (w/v) NaCl, and at pH 7. The major isoprenoid quinone was menaquinone-6 (MK-6). The dominant polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, and unidentified polar lipids. The predominant cellular fatty acids were C16:0, C18:1 ω9c, and C18:0. The genomic DNA G+C content of strain LHWP3T was 53.0 mol%. Based on polyphasic taxonomic analyses, strain LHWP3T should be classified as a novel species in the genus Rhodopirellula in the family Planctomycetaceae, for which the name Rhodopirellula rosea sp. nov. is proposed. The type strain is LHWP3T (=KACC 15560T =JCM 17759T).

Halorubrum cibi sp. nov., an Extremely Halophilic Archaeon from Salt-Fermented Seafood

노성운,배진우 한국미생물학회 2009 The journal of microbiology Vol.47 No.2

Strain B31T is a Gram-staining-negative, motile, and extremely halophilic archaeon that was isolated from salt-fermented seafood. Its morphology, physiology, biochemical features, and 16S rRNA gene sequence were determined. Phylogenetic analysis of its 16S rRNA gene sequence and composition of its major polar lipids placed this archaeon in the genus Halorubrum of the family Halobacteriaceae. Strain B31T showed 97.3, 97.2, and 96.9% 16S rRNA similarity to the type strains of Halorubrum alkaliphilum, Hrr. tibetense, and Hrr. vacuolatum, respectively. Its major polar lipids were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me) and sulfated diglycosyl diether (S-DGD). Genomic DNA from strain B31T has a 61.7 mol% G+C content. Analysis of 16S rRNA gene sequences, as well as physiological and biochemical tests, identified genotypic and phenotypic differences between strain B31T and other Halorubrum species. The type strain of the novel species is B31T (=JCM 15757T =DSM 19504T).

Aliihoeflea aestuarii gen. nov., sp. nov., a Novel Bacterium Isolated from Tidal Flat Sediment

노성운,김경호,Young-Do Nam,Ho-Won Chang,Min-Soo Kim,신기선,윤정훈,오희목,Jin-Woo Bae 한국미생물학회 2008 The journal of microbiology Vol.46 No.5

A novel Gram-negative and rod-shaped bacterium, designated N8^T, was isolated from tidal flat sediment. Phylogenetic analysis based on 16S rRNA gene sequences showed that N8^T strain is associated with thefamily Phyllobacteriaceae: two uncultured clones (98.4 and 99.8% 16S rRNA gene sequence similarity) andthe genus Mesorhizobium (≤97.0%). The novel strain formed a separate clade with uncultured clones in thephylogenetic tree based on 16S rRNA gene sequences. Cellular fatty acid profiles predominately comprisedC_18:1 ω7c and C19:0 cyclo ω8c. The major isoprenoid quinone is ubiquinone-10 and genomic DNA G+C contentis 53.4 mol%. The polyphasic taxonomic study indicates that the novel strain N8^T represents a novelspecies of the new genus in the family Phyllobacteriaceae, named Aliihoeflea aestuarii. The type strain isN8^T (= KCTC 22052^T= JCM 15118^T= DSM 19536^T).

Luteimonas aestuarii sp. nov., Isolated from Tidal Flat Sediment

노성운,김경호,Young-Do Nam,Ho-Won Chang,Min-Soo Kim,윤정훈,오희목,Jin-Woo Bae 한국미생물학회 2008 The journal of microbiology Vol.46 No.5

A novel bacterium B9^T was isolated from tidal flat sediment. Its morphology, physiology, biochemical features,and 16S rRNA gene sequence were characterized. Colonies of this strain are yellow and the cells areGram-negative, rod-shaped, and do not require NaCl for growth. The 16S rRNA gene sequence similarityindicated that strain B9^T is associated with the genus Lysobacter (≤ 97.2%), Xanthomonas (≤ 96.8%),Pseudomonas (≤ 96.7%), and Luteimonas (≤ 96.0%). However, within the phylogenetic tree, this novel strainshares a branching point with the species Luteimonas composti CC-YY255^T (96.0%). The DNA-DNA hybridizationexperiments showed a DNA-DNA homology of 23.0% between strain B9^T and Luteimonas mephitisB1953/27.1^T. The G+C content of genomic DNA of the type strain is 64.7 mol% (SD, 1.1). The predominantfatty acids are iso-C_11:0, iso-C_15:0, iso-C_16:0, iso-C_17:0, iso-C_17:1 ω9c, and iso-C_11:0 3-OH. Combined analysisof the 16S rRNA gene sequences, fatty acid profile, and results from physiological and biochemical testsindicated that there is genotypic and phenotypic differentiation of the isolate from other Luteimonasspecies. For these reasons, strain B9^T was proposed as a novel species, named Luteimonas aestuarii. Thetype strain of the new species is B9^T (= KCTC 22048^T, DSM 19680^T).

미육군 교육용 AA온라인게임 품질모형에 의한 버그시험 평가

노성운(SungWoon Noh),최성(Sung Choi) 한국정보과학회 2005 정보과학회지 Vol.23 No.3

A report of 37 unrecorded anaerobic bacterial species isolated from the Geum River in South Korea

이창수,김연비,노성운,김주석,안승우,송혜선,노성운,김준용 국립생물자원관 2020 Journal of species research Vol.9 No.2

A total of 37 anaerobic bacteria strains within the classes Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacteroidia, Flavobacteriia, Bacilli, Clostridia, and Fusobacteriia were isolated from freshwater and sediment of the Geum River in Korea. The unreported species were related with Rhizobium and Oleomonas of the class Alphaproteobacteria; Acidovorax, Pseudogulbenkiania, and Aromatoleum of the class Betaproteobacteria; Tolumonas, Aeromonas, Cronobacter, Lonsdalea, and Phytobacter of the class Gammaproteobacteria; Bacteroides, Dysgonomonas, Macellibacteroides, and Parabacteroides of the class Bacteroidia; Flavobacterium of the class Flavobacteriia; Bacillus and Paenibacillus of the class Bacilli; Clostridium, Clostridioides, Paraclostridium, Romboutsia, Sporacetigenium, and Terrisporobacter of the class Clostridia; and Cetobacterium and Ilyobacter of the class Fusobacteriia. A total of 37 strains, with >98.7% 16S rRNA gene sequence similarity with validly published bacterial species, but not reported in Korea, were determined to be unrecorded anaerobic bacterial species in Korea.

Halomonas jeotgali sp. nov., a New Moderate Halophilic Bacterium Isolated from a Traditional Fermented Seafood

김민수,노성운,배진우 한국미생물학회 2010 The journal of microbiology Vol.48 No.3

A moderate halophilic, Gram-negative, non-motile, rod-shape, and aerobe designated as strain HwaT was isolated from traditional fermented Korean seafood, which presented as a single cell or paired cells. Optimal growth occurred at 25°C in 10% (w/v) salts at pH 7.0-8.0; however, growth occurred in a temperature range of 10-32°C, a salts concentration of 5-25% (w/v) and pH 5.0-10.0. Tests for oxidase and catalase were positive. The cells produced poly-β-hydroxybutyric acid, but not exopolysaccharide. Based on the 16S rRNA gene sequence, not only was there low similarity between strain HwaT and all other species (94.1% similarity with H. subglaciescola DSM 4683T, 94.0% similarity with H. sulfidaeris Esulfide1T, 93.6% similarity with H. cerina SP4T and 93.0% similarity with H. halodurans DSM 5160T), but the phylogenetic analysis revealed that the isolate may be classified as a novel species belonging to the genus Halomonas in the class Gammaproteobacteria. The predominant fatty acids of strain HwaT were C18:1 ω7c, C16:0, C12:0 3-OH and C16:1 ω7c/C15:0 iso 2-OH. The DNA G+C content was calculated as 61.7 mol%. Based on phenotypic, genotypic, and phylogenetic characteristics, it is proposed that the strain designated as HwaT be assigned to the genus Halomonas as Halomonas jeotgali sp. nov. (=KCTC 22487T =JCM 15645T).

Paenibacillus marinisediminis sp. nov., a Bacterium Isolated from Marine Sediment

이해원,노성운,Kyung June Yim,신나리,이지나,원태웅,Joon-Yong Kim,현동욱,김대경,배진우 한국미생물학회 2013 The journal of microbiology Vol.51 No.3

A Gram-negative, nonmotile, endospore-forming, rod-shaped bacterial strain LHW35T, which belonged to the genus Paenibacillus, was isolated from marine sediment collected from the south coast of the Republic of Korea. A phylogenetic analysis of 16S rRNA gene sequences indicated that strain LHW35T was most closely related to Paenibacillus taiwanensis G-soil-2-3T (97.2% similarity). The optimal growth conditions for strain LHW35T were 37°C, pH 6.0, and 0% (w/v) NaCl. The main isoprenoid quinone was menaquinone-7 (MK-7) and the major polyamine was spermidine. The diamino acid present in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major fatty acids were anteiso-C15:0 and C16:0. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, unidentified aminohospholipids, unidentified phospholipids, and unidentified polar lipids. A DNA-DNA hybridization experiment using the type strain of P. taiwanensis indicated <40% relatedness. The DNA G+C content was 45.0 mol%. Based on these phylogenetic, genomic, and phenotypic analyses, strain LHW35T should be classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus marinisediminis sp. nov. is proposed. The type strain is LHW35T (=KACC 16317T =JCM 17886T).