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RISS 인기검색어
- 검색키워드
- 저자 : Xiangping Li (검색결과 5 건)
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Optical temperature sensing properties of Yb3+/Tm3+ co-doped NaLuF4 crystals
Lili Tong,Xiangping Li,Ruinian Hua,Lihong Cheng,Jiashi Sun,Jinsu Zhang,Sai Xu,Hui Zheng,Yanqiu Zhang,Baojiu Chen 한국물리학회 2017 Current Applied Physics Vol.17 No.7
Yb3þ/Tm3þ and Yb3þ/Er3þ co-doped NaLuF4 crystals were synthesized by a facile hydrothermal method. The optical temperature sensing properties of Tm3þ based upon its two thermally coupled energy levels 3F2, 3 and 3H4 were systematically investigated by means of fluorescence intensity ratio (FIR) technique. The 980 nm laser-induced thermal effect on Tm3þ doped NaLuF4 crystals was studied by using Er3þ doped sample as thermal probe. The temperature sensitivity of Tm3þ in NaLuF4: Yb3þ/Tm3þ crystals shows a nonlinear dependence on temperature, and the maximum value is about 0.00045 K『1 at 600 K. The accuracy and reliability of the optical thermometry based on Tm3þ in NaLuF4: Yb3þ/Tm3þ crystals has been checked by using Yb3þ/Er3þ co-doped sample as temperature sensing unit. The results demonstrate that NaLuF4: Yb3þ/Tm3þ crystals have good sensing stability and may have potential application for the optical thermometry.
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An Improved Tet-on System to Tightly Conditionally Regulate Reporter Gene Expression
Yanping Ren,Xiangping Li,Qingyou Liu,Yanfei Deng,Deshun Shi 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1
Reporter genes are often used as markers totrack the integration and expression of target genes inanimal genetic engineering. To avoid potential side effectsfrom reporter genes, in this study an improved Tet-onsystem was developed to control reporter gene expression,and its effectiveness was explored in transgenic cells. First,the rtTA protein was fused with Tat and NLS proteins toobtain the prokaryotic expression vector pET32a-Tat-rtTANSL. A eukaryotic transgenic vector was constructed, p-HS4-BPA-TmA-HS4, in which the reporter (mCherry) andtarget (PRL) genes were promoted by TRE and BCN,respectively. After confirming the functionality of thetransgenic vector, purified rtTA protein and Dox wereadded to induce expression of the mCherry gene. Theoptimal amount of purified rtTA protein, its influence ontarget gene expression, and the time of rtTA protein actionwere each investigated separately. The results showed thatrtTA protein was expressed in transformed E. coli with IPTGinduction. TRE could promote mCherry gene expressionby co-transfecting the constructed transgenic vector andprtTA plasmid. When purified rtTA protein and Dox wereadded, red fluorescence was observed in Bcap-37 cellstransfected with the p-HS4-BPA-TmA-HS4 vector, and theexogenous PRL gene was expressed regardless of mCherrygene expression. The optimal amount of rtTA protein was16 μg/mL, and it needed about 6 h to promote mCherrygene expression in transfected cells. These resultsdemonstrate that the expression of the mCherry reportergene can be tightly and conditionally regulated by our Tetonsystem.
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Chen Po-Ju,Ren Lianping,Xiangping Li,Yang Fiona X. 한국마케팅과학회 2022 마케팅과학연구 Vol.32 No.1
This introduction reviews current issues in tourist behaviors and retailing. The article furthers the retailing shopping framework and its application to consumer behavior in general and tourism and retailing in particular. A summary of each of the six articles that follow appears. The article extends a note of appreciation to the members of the special editorial board for this issue.
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Analysis of Peripheral B Cell Subsets in Patients With Allergic Rhinitis
Jing Luo,Huanhuan Guo,Zhuofu Liu,Tao Peng,Xianting Hu,Miaomiao Han,Xiangping Yang,Xuhong Zhou,Huabin Li 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.3
Purpose: Recent evidence suggests that B cells can both promote and inhibit the development and progression of allergic disease. However, the characteristics of B cell subsets in patients with allergic rhinitis (AR) have not been well documented. This study aimed to analyze the characteristics of B cell subsets in the peripheral blood of AR patients. Methods: Forty-seven AR patients and 54 healthy controls were enrolled in this study, and the B cell subsets in peripheral blood of all subjects were analyzed by flow cytometry. Moreover, the serum total immunoglobulin E (IgE) and IgE concentrations secreted into the cultured peripheral blood mononuclear cells (PBMCs) were measured by using enzyme-linked immunosorbent assay. Results: We found the peripheral blood of AR patients contained higher percentages of memory B cells, plasma cells, and CD19+CD24hiCD27+ regulatory B cells (Bregs) than those of age-matched healthy controls (P<0.05), while the percentages of naïve B cells and CD19+CD24hiCD38hi Bregs were significantly lower in AR patients than in healthy individuals (P<0.05). In addition, the serum total IgE and IgE concentrations secreted into the cultured PBMCs were elevated in AR patients than in the healthy controls (P<0.05). Conclusions: Our findings indicate that AR patients were characterized by increase in terminally differentiated memory B cells or plasma cells and decreases in CD19+CD24hiCD38hi Breg cells in the peripheral blood.
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Zhaocheng Xiong,Haihang Zhang,Ben Huang,Qingyou Liu,Yingqun Wang,Deshun Shi,Xiangping Li 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.11
Objective: The aim of the current study is to investigate the relationship between prohibitin (PHB), capping actin protein of muscle Z-line beta subunit (CAPZB), and tektin-2 (TEKT2) and sperm motility in Murrah buffalo. Methods: We collected the high-motility and low-motility semen samples, testis, ovary, muscle, kidney, liver, brain and pituitary from Murrah buffalo, and analysed the expression of PHB, CAPZB, and TEKT2 in mRNA (message RNA) and protein level. Results: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) result showed that the expression of PHB was higher and CAPZB, TEKT2 were specifically expressed in testis as compared to the other 6 tissues, and that in testis, the expression of TEKT2 was higher than that of CAPZB and PHB. Immunohistochemistry test revealed that all three genes were located on the convoluted seminiferous tubule and enriched in spermatogenic cells. Both qRT-PCR and Western Blot results showed that the expression levels of PHB, CAPZB, and TEKT2 were significantly lower in the low-motility semen group compared to the high-motility semen group (p<0.05). Conclusion: The expression levels of PHB, CAPZB, and TEKT2 in Murrah buffalo sperm have a high positive correlation with sperm motility. And the three genes may be potential molecular markers for the decline of buffalo sperm motility.
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