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- 저자 : Huanhuan Guo (검색결과 4 건)
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Guo, Kai,Jiang, Naibin,Qi, Huanhuan,Feng, Zhipeng,Wang, Yang,Tan, Wei Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.6
The tubes in a heat exchanger, such as a steam generator (SG), are subjected to crossflow, and interaction between tubes and supports can happen, which can cause fretting wear of tubes. Although many experiments and models have been established, some detailed mechanisms are still not sufficiently clear. In this work, more attention is paid to obtain the regulation of impact and sliding in the complex process and many factors, such as excitation forces and clearances. The responses and contact forces were analyzed to obtain clear understanding of the influences of these factors. Room temperature tests in the air were established. The results show that the effect of clearance on the normal work rate is not monotonous and instead has two peaks. The force ratio can influence the normal work rate by changing the distribution of contact angles, which can result in higher sliding in the contact process. Fretting wear tests are conducted, and the wear surfaces are analyzed by a scanning electron microscope (SEM) and energy dispersive X-ray spectrometer (EDX). The results of this work can serve as a reference for impactsliding contact analysis between AVBs and tubes in steam generators.
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Analysis of Peripheral B Cell Subsets in Patients With Allergic Rhinitis
Jing Luo,Huanhuan Guo,Zhuofu Liu,Tao Peng,Xianting Hu,Miaomiao Han,Xiangping Yang,Xuhong Zhou,Huabin Li 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.3
Purpose: Recent evidence suggests that B cells can both promote and inhibit the development and progression of allergic disease. However, the characteristics of B cell subsets in patients with allergic rhinitis (AR) have not been well documented. This study aimed to analyze the characteristics of B cell subsets in the peripheral blood of AR patients. Methods: Forty-seven AR patients and 54 healthy controls were enrolled in this study, and the B cell subsets in peripheral blood of all subjects were analyzed by flow cytometry. Moreover, the serum total immunoglobulin E (IgE) and IgE concentrations secreted into the cultured peripheral blood mononuclear cells (PBMCs) were measured by using enzyme-linked immunosorbent assay. Results: We found the peripheral blood of AR patients contained higher percentages of memory B cells, plasma cells, and CD19+CD24hiCD27+ regulatory B cells (Bregs) than those of age-matched healthy controls (P<0.05), while the percentages of naïve B cells and CD19+CD24hiCD38hi Bregs were significantly lower in AR patients than in healthy individuals (P<0.05). In addition, the serum total IgE and IgE concentrations secreted into the cultured PBMCs were elevated in AR patients than in the healthy controls (P<0.05). Conclusions: Our findings indicate that AR patients were characterized by increase in terminally differentiated memory B cells or plasma cells and decreases in CD19+CD24hiCD38hi Breg cells in the peripheral blood.
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Mingguan Yang,Laifeng Lu,Jing Pang,Yiling Hu,Qingbin Guo,Zhenjing Li,Shufen Wu,Huanhuan Liu,Changlu Wang 한국미생물학회 2019 The journal of microbiology Vol.57 No.5
Aspergillus flavus is a saprophytic fungus that contaminates crops with carcinogenic aflatoxin. In the present work, the antifungal effects of volatile organic compounds (VOCs) from Streptomyces alboflavus TD-1 against A. flavus were investigated. VOCs from 8-day-old wheat bran culture of S. alboflavus TD-1 displayed strong inhibitory effects against mycelial growth, sporulation, and conidial germination of A. flavus. Severely misshapen conidia and hyphae of A. flavus were observed by scanning electron microscopy after exposure to VOCs for 6 and 12 h, respectively. Rhodamine 123 staining of mitochondria indicated that mitochondria may be a legitimate antifungal target of the VOCs from S. alboflavus TD-1. Furthermore, the VOCs effectively inhibited aflatoxin B1 production by downregulating genes involved in aflatoxin biosynthesis. Dimethyl trisulfide and benzenamine may play important roles in the suppression of A. flavus growth and production of aflatoxin. The results indicate that VOCs from S. alboflavus TD-1 have tremendous potential to be developed as a useful bio-pesticide for controlling A. flavus.
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Cuihua Xin,Ruikun Hou,Fei Wu,Yibo Zhao,Huanhuan Xiao,Wantong Si,Md Emran Ali,Lu Cai,Jiangbo Guo 한국식물학회 2015 Journal of Plant Biology Vol.58 No.6
China is the leading producer of potatoes (Solanum tuberosum) in the world. However, low-temperature stress can severely restrict the yield and quality of potato crops in that country. We used methylation-sensitive amplified polymorphisms (MSAPs) to analyze how such stress might alter the status of cytosine methylation in potato cultivar ‘Atlantic’. Over 2400 loci were identified and assessed for DNA methylation in chilling-treated and control plants. The level of cytosine methylation increased from 37.71% to 56.51% after 48 h of treatment but then decreased to 42.14% after 60 h of chilling. Stress-induced changes in the banding patterns of DNA methylation primarily resulted from full methylation of CG (internal cytosine of 5’-CCGG-3’) on double DNA strands. We sequenced 78 polymorphic DNA fragments and blasted them against NCBI. Expression patterns of 4 genes were then assessed via semi-quantitative RT-PCR. The expression patterns of genes related to chilling stress appeared to be regulated by the methylation or demethylation status in potato.
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