Handheld photoacoustic tomography probe built using optical-fiber parallel acoustic delay lines

Cho, Young,Chang, Cheng-Chung,Yu, Jaesok,Jeon, Mansik,Kim, Chulhong,Wang, Lihong V.,Zou, Jun SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS 2014 JOURNAL OF BIOMEDICAL OPTICS Vol.19 No.8

Effects of Long-Term Fertilizer Practices on Rhizosphere Soil Autotrophic CO₂-Fixing Bacteria under Double Rice Ecosystem in Southern China

( Haiming Tang ),( Li Wen ),( Lihong Shi ),( Chao Li ),( Kaikai Cheng ),( Weiyan Li ),( Xiaoping Xiao ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.10

Soil autotrophic bacterial communities play a significant role in the soil carbon (C) cycle in paddy fields, but little is known about how rhizosphere soil microorganisms respond to different long-term (35 years) fertilization practices under double rice cropping ecosystems in southern China. Here, we investigated the variation characteristics of rhizosphere soil RubisCO gene cbbL in the double rice ecosystems of in southern China where such fertilization practices are used. For this experiment we set up the following fertilizer regime: without any fertilizer input as a control (CK), inorganic fertilizer (MF), straw returning (RF), and organic and inorganic fertilizer (OM). We found that abundances of cbbL, 16S rRNA genes and RubisCO activity in rhizosphere soil with OM, RF and MF treatments were significantly higher than that of CK treatment. The abundances of cbbL and 16S rRNA genes in rhizosphere soil with OM treatment were 5.46 and 3.64 times higher than that of CK treatment, respectively. Rhizosphere soil RubisCO activity with OM and RF treatments increased by 50.56 and 45.22%, compared to CK treatment. Shannon and Chao1 indices for rhizosphere soil cbbL libraries with RF and OM treatments increased by 44.28, 28.56, 29.60, and 23.13% compared to CK treatment. Rhizosphere soil cbbL sequences with MF, RF and OM treatments mainly belonged to Variovorax paradoxus, uncultured proteobacterium, Ralstonia pickettii, Thermononospora curvata, and Azoarcus sp.KH33C. Meanwhile, cbbL-carrying bacterial composition was obviously influenced by soil bulk density, rhizosphere soil dissolved organic C, soil organic C, and microbial biomass C contents. Fertilizer practices were the principal factor influencing rhizosphere soil cbbL-carrying bacterial communities. These results showed that rhizosphere soil autotrophic bacterial communities were significantly changed under conditions of different long-term fertilization practices Therefore, increasing rhizosphere soil autotrophic bacteria community with crop residue and organic manure practices was found to be beneficial for management of double rice ecosystems in southern China.

Co-precipitation synthesis and characterization of Bi3+, Eu3+ co-doped nanocrystal Gd2WO6 phosphors.

Tian, Yue,Chen, Baojiu,Hua, Ruinian,Cheng, Lihong,Zhong, Haiyang,Sun, Jiashi,Wang, Bo,Wan, Jing,Lu, Weili,Jang, Kiwan American Scientific Publishers 2010 Journal of nanoscience and nanotechnology Vol.10 No.3

<P>Eu3+ single-doped and Bi3+/Eu3+ co-doped nanocrystal Gd2WO6 phosphors were successfully synthesized via a co-precipitation reaction. The structure and morphology of the phosphors were characterized by using X-ray powder diffraction (XRD) and field emission scanning electron microscopy (FE-SEM). The influence of Bi(3+)-doping concentration on the excitation and emission spectra was studied. It was found that the introduction of Bi3+ can greatly affect the charge transfer band and the luminescence intensity of Eu3+ but does not cause a change in the profile of emission spectra of Eu3+.</P>

Air Embolism during Upper Endoscopy: A Case Report

Yin Fang,Junbei Wu,Feng Wang,Lihong Cheng,Yunhong Lu,Xiaofei Cao 대한소화기내시경학회 2019 Clinical Endoscopy Vol.52 No.4

Air embolism is a rare complication of upper endoscopy and potentially causes life-threatening events. A 67-year-old man with ahistory of surgery of cardiac carcinoma and pancreatic neuroendocrine tumor underwent painless upper endoscopy because oftarry stools. During the procedure, air embolism developed, which caused decreased pulse oxygen saturation and delayed sedationrecovery. He recovered with some weakness of the left upper limb in the intensive care unit without hyperbaric oxygen therapy. Theetiology, clinical manifestations, and treatments of air embolism are discussed based on the literature reports. Although air embolism isuncommon in endoscopic examinations, the patients’ outcomes could be improved if clinicians are alert to this potential complication,and promptly start proper diagnostic and therapeutic measures.

Optical temperature sensing properties of Yb3+/Tm3+ co-doped NaLuF4 crystals

Lili Tong,Xiangping Li,Ruinian Hua,Lihong Cheng,Jiashi Sun,Jinsu Zhang,Sai Xu,Hui Zheng,Yanqiu Zhang,Baojiu Chen 한국물리학회 2017 Current Applied Physics Vol.17 No.7

Yb3þ/Tm3þ and Yb3þ/Er3þ co-doped NaLuF4 crystals were synthesized by a facile hydrothermal method. The optical temperature sensing properties of Tm3þ based upon its two thermally coupled energy levels 3F2, 3 and 3H4 were systematically investigated by means of fluorescence intensity ratio (FIR) technique. The 980 nm laser-induced thermal effect on Tm3þ doped NaLuF4 crystals was studied by using Er3þ doped sample as thermal probe. The temperature sensitivity of Tm3þ in NaLuF4: Yb3þ/Tm3þ crystals shows a nonlinear dependence on temperature, and the maximum value is about 0.00045 K『1 at 600 K. The accuracy and reliability of the optical thermometry based on Tm3þ in NaLuF4: Yb3þ/Tm3þ crystals has been checked by using Yb3þ/Er3þ co-doped sample as temperature sensing unit. The results demonstrate that NaLuF4: Yb3þ/Tm3þ crystals have good sensing stability and may have potential application for the optical thermometry.

Effects of Dietary L-carnosine and Alpha-lipoic Acid on Growth Performance, Blood Thyroid Hormones and Lipid Profiles in Finishing Pigs

Yinghui Bao,Chunqi Gao,Wenbo Hao,Cheng Ji,Lihong Zhao,Jianyun Zhang,Tao Liu,Qiugang Ma 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.10

The present study was conducted to determine the effects of L-carnosine (LC) and/or alpha-lipoic acid (ALA) supplementation on growth performance, blood thyroid hormones and lipid profiles in finishing pigs. A total of 40 (Landrace×Yorkshire) pigs with an initial body weight of 57.93±3.14 kg were randomly allocated to 4 experimental diets using a 2×2 factorial arrangement with 2 LC supplemental levels (0 or 0.1%) and 2 ALA supplemental levels (0 or 0.03%) in basal diets. The results showed that pigs fed LC-supplemented diets increased final live weight, average daily gain, and average daily feed intake compared to those of pigs fed without LC-supplemented diets (p<0.05). Dietary supplementation with ALA did not affect the growth performance and carcass traits of pigs (p>0.05). Additionally, LC supplementation increased serum triiodothyronine, thyroxine levels, and ALA supplementation increased serum triiodothyronine levels (p<0.05). Serum total cholesterol and triglycerides levels were significantly decreased in LC and ALA supplemented groups, respectively (p<0.05). Moreover, serum low density lipoprotein cholesterol levels were lower in the ALAsupplemented groups than those of pigs fed without ALA-supplemented diets (p<0.05). However, no significant LC×ALA interaction effect on growth performance, blood thyroid hormones and lipid profiles was found. This study suggested that dietary supplementation of LC resulted in better growth performance compared to that of ALA supplementation. L-carnosine and/or ALA supplementation positively modified blood lipid profiles, which may have the potential to prevent cardiovascular diseases.

Effects of Dietary L-carnosine and Alpha-lipoic Acid on Growth Performance, Blood Thyroid Hormones and Lipid Profiles in Finishing Pigs

Bao, Yinghui,Gao, Chunqi,Hao, Wenbo,Ji, Cheng,Zhao, Lihong,Zhang, Jianyun,Liu, Tao,Ma, Qiugang Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.10

The present study was conducted to determine the effects of L-carnosine (LC) and/or alpha-lipoic acid (ALA) supplementation on growth performance, blood thyroid hormones and lipid profiles in finishing pigs. A total of 40 ($Landrace{\times}Yorkshire$) pigs with an initial body weight of $57.93{\pm}3.14kg$ were randomly allocated to 4 experimental diets using a $2{\times}2$ factorial arrangement with 2 LC supplemental levels (0 or 0.1%) and 2 ALA supplemental levels (0 or 0.03%) in basal diets. The results showed that pigs fed LC-supplemented diets increased final live weight, average daily gain, and average daily feed intake compared to those of pigs fed without LC-supplemented diets (p<0.05). Dietary supplementation with ALA did not affect the growth performance and carcass traits of pigs (p>0.05). Additionally, LC supplementation increased serum triiodothyronine, thyroxine levels, and ALA supplementation increased serum triiodothyronine levels (p<0.05). Serum total cholesterol and triglycerides levels were significantly decreased in LC and ALA supplemented groups, respectively (p<0.05). Moreover, serum low density lipoprotein cholesterol levels were lower in the ALA-supplemented groups than those of pigs fed without ALA-supplemented diets (p<0.05). However, no significant $LC{\times}ALA$ interaction effect on growth performance, blood thyroid hormones and lipid profiles was found. This study suggested that dietary supplementation of LC resulted in better growth performance compared to that of ALA supplementation. L-carnosine and/or ALA supplementation positively modified blood lipid profiles, which may have the potential to prevent cardiovascular diseases.

Effect of Dietary Phytase Transgenic Corn on Physiological Characteristics and the Fate of Recombinant Plant DNA in Laying Hens

Gao, Chunqi,Ma, Qiugang,Zhao, Lihong,Zhang, Jianyun,Ji, Cheng Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.1

The study aimed to evaluate the potential effects of feeding with phytase transgenic corn (PTC) on organ weight, serum biochemical parameters and nutrient digestibility, and to determine the fate of the transgenic DNA in laying hens. A total of 144 50-week-old laying hens were grouped randomly into 2 treatments, with 8 replicates per treatment and 9 hens per replicate. Each treatment group of hens was fed with diets containing 62.4% non-transgenic conventional corn (CC) or PTC for 16 weeks. The phytase activity for CC was 37 FTU/kg of DM, whereas the phytase activity for PTC was 8,980 FTU/kg of DM. We observed that feeding PTC to laying hens had no adverse effect on organ weight or serum biochemical parameters (p>0.05). A fragment of a poultry-specific ovalbumin gene (ov) was amplified from all tissues of hens showing that the DNA preparations were amenable to PCR amplification. Neither the corn-specific invertase gene (ivr) nor the transgenic phyA2 gene was detected in the breast muscle, leg muscle, ovary, oviduct and eggs. The digestibility data revealed no significant differences between the hens that received the CC- and PTC-based diets in the digestibility of DM, energy, nitrogen and calcium (p>0.05). Phosphorus digestibility of hens fed the PTC-based diet was greater than that of hens fed the CC-based diet (58.03% vs 47.42%, p<0.01). Based on these results, it was concluded that the PTC had no deleterious effects on the organ weight or serum biochemical parameters of the laying hens. No recombinant phyA2 gene was detected in muscle tissues and reproductive organs of laying hens. The novel plant phytase was efficacious in improving the phosphorus digestibility of laying hens.

Effects of Dietary Acetyl-L-Carnitine on Meat Quality and Lipid Metabolism in Arbor Acres Broilers

Zhang, Yong,Ma, Qiugang,Bai, Xiumei,Zhao, Lihong,Wang, Qiang,Ji, Cheng,Liu, Laiting,Yin, Haicheng Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.12

An experiment was conducted to evaluate the effects of dietary acetyl-L-carnitine (ALC) on growth performance, carcass characteristics, meat quality and lipid metabolism in broilers. A total of 240 one-day-old male Arbor Acres broilers were randomly allocated to 4 dietary treatments (0, 300, 600, and 900 mg/kg dietary ALC supplementation, respectively). Compared with the control treatment, addition of ALC resulted in lower (linear effect, p<0.05) ADG and AFI. Abdominal fat percentage decreased (linear effect, p<0.05) as dietary ALC was increased, but there was no effect on dressing percentage, breast muscle percentage or thigh muscle percentage. Breast muscle pH value 24 h post-mortem increased (linear effect, p<0.05), but there were no significant differences among treatments. However, thigh muscle pH value increased (linear effect, p<0.05) as dietary ALC was increased. Breast and thigh muscle $a^*$ values increased (linear effect, p<0.05), and breast and thigh muscle $b^*$ values decreased (linear effect, p<0.05) with increased ALC in the diet. In addition, breast and thigh muscle shear force value decreased (linear effect, p<0.05) as dietary ALC was increased. Total cholesterol, triglyceride, low-density lipoprotein cholesterol and lipoprotein lipase decreased (linear effect, p<0.05) and free fatty acid and lipase in serum increased (linear effect, p<0.05) with increased ALC in diets.

Effects of short-term soil tillage management on activity and community structure of denitrifiers under double-cropping rice field

Haiming Tang,Chao Li,Kaikai Cheng,Lihong Shi,Li Wen,Xiaoping Xiao,Yilan Xu,Weiyan Li,Ke Wang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.11

Soil physical and chemical characteristics, soil potential denitrification rates (PDR), community composition and nirK-, nirS- and nosZ-encoding denitrifiers were studied by using MiSeq sequencing, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment polymorphism (T-RFLP) technologies base on short-term (5-year) tillage field experiment. The experiment included four tillage treatments: conventional tillage with crop residue incorporation (CT), rotary tillage with crop residue incorporation (RT), no-tillage with crop residue retention (NT), and rotary tillage with crop residue removed as control (RTO). The results indicated that soil organic carbon, total nitrogen and NH4 +-N contents were increased with CT, RT and NT treatments. Compared with RTO treatment, the copies number of nirK, nirS and nosZ in paddy soil with CT, RT and NT treatments were significantly increased. The principal coordinate analysis indicated that tillage management and crop residue returning management were the most and the second important factors for the change of denitrifying bacteria community, respectively. Meanwhile, this study indicated that activity and community composition of denitrifiers with CT, RT and NT treatments were increased, compared with RTO treatment. This result showed that nirK, nirS and nosZ-type denitrifiers communities in crop residue applied soil had higher species diversity compared with crop residue removed soil, and denitrifying bacteria community composition were dominated by Gammaproteobacteria, Deltaproteobacteria, and Betaproteobacteria. Therefore, it is a beneficial practice to increase soil PDR level, abundance and community composition of nitrogen-functional soil microorganism by combined application of tillage with crop residue management.