HPLC Determination of Malondialdehyde in ECV304 Cell Culture Medium for Measuring the Antioxidant Effect of Vitexin-4"-O-glucoside

Ying, Xi-Xiang,Li, Hai-Bo,Chu, Zheng-Yun,Zhai, Yan-Jun,Leng, Ai-Jing,Liu, Xun,Xin, Chun,Zhang, Wen-Jie,Kang, Ting-Guo 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.7

To investigate the antioxidant effect of vitexin-4"-O-glucoside, a flavone glycoside, isolated from the leaves of Crataegus pinnatifida Bge. var. major, we developed a simple and sensitive high-performance liquid chromatography (HPLC) method to determine levels of malondialdehyde (MDA) in ECV304 cell culture medium after induction by tert-butyl-hydroperoxide (TBHP). The preparation of analyzed samples involved a one-step derivatization with thiobarbituric acid (TBA). HPLC analysis was performed on a $Synergi^{TM}$ Hydro-RP, a polar end-capped $C_{18}$ column ($250{\times}4.6\;mm$, $4\;{\mu}m$), using an acetonitrile-ammonium acetate aqueous solution (10 mM, pH 6.8) as the mobile phase under linear gradient conditions with UV detection at 532 nm. The calibration curve was linear over $0.0125-1.25\;{\mu}M$ MDA (r=0.9951). Relative standard deviations (RSDs) of intra-day and inter-day precision were less than 6.1% and 5.0%, respectively. The mean recovery was $96.9\;{\pm}\;1.6%$. The lower limit of quantification (LLOQ) of MDA was $0.0125\;{\mu}M$. This chromatographic method was successfully applied to investigating the in vitro antioxidant effect of vitexin-4"-O-glucoside. Vitexin-4"-O-glucoside (120 M) protected ECV304 cells from peroxidation induced by TBHP.

HPLC Determination of Malondialdehyde in ECV304 Cell Culture Medium for Measuring the Antioxidant Effect of Vitexin-4"-O-glucoside

Xi-xiang Ying,Hai-bo Li,Zheng-yun Chu,Yan-jun Zhai,Ai-jing Leng,Xun Liu,Wen-jie Zhang,Ting-guo Kang,Chun Xin 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.7

To investigate the antioxidant effect of vitexin-4"-O-glucoside, a flavone glycoside, isolated from the leaves of Crataegus pinnatifida Bge. var. major, we developed a simple and sensitive high-performance liquid chromatography (HPLC) method to determine levels of malondialdehyde (MDA) in ECV304 cell culture medium after induction by tert-butyl-hydroperoxide (TBHP). The preparation of analyzed samples involved a one-step derivatization with thiobarbituric acid (TBA). HPLC analysis was performed on a SynergiTM Hydro-RP, a polar end-capped C18 column (250×4.6 mm, 4 μm), using an acetonitrile-ammonium acetate aqueous solution (10 mM, pH 6.8) as the mobile phase under linear gradient conditions with UV detection at 532 nm. The calibration curve was linear over 0.0125-1.25 μM MDA (r = 0.9951). Relative standard deviations (RSDs) of intra-day and inter-day precision were less than 6.1% and 5.0%, respectively. The mean recovery was 96.9 ± 1.6%. The lower limit of quantification (LLOQ) of MDA was 0.0125 μM. This chromatographic method was successfully applied to investigating the in vitro antioxidant effect of vitexin-4"-O-glucoside. Vitexin-4"-O-glucoside (120 M) protected ECV304 cells from peroxidation induced by TBHP.

Two kinds of behavior of fruit peel coagulant in treating low carbon source wastewaters

Ying Fu,Qinghe Yue,Shuyuan Luo,Xi Tian,Jiewen Zheng,Yanting Yang 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.4

Based on present serious phenomenon of insufficient carbon source in municipal sewage in China, the two kinds of effect of hybrid-fruit-peel (HFP) coagulant (coagulation and external carbon source (ECS)) on treating low carbon source wastewater was studied, mainly compared with that of sodium acetate (NaAc). The feasibility of HFP as an ECS was researched, and then its coagulation behavior, sludge properties, nitrogen removal were studied mainly with aeration mode and sequencing batch reactor (SBR), respectively. The results showed the ration of biochemical oxygen demand (BOD5) to chemical oxygen demand (COD) released from HFP was 0.67−0.76, within the scope of easily biodegradable. Within a short aeration time, HFP posed obvious coagulation behavior. HFP sludge gave larger extracellular polymer (EPS) and lower dehydrogenase activity (DHA) than NaAc. For HFP, much larger size sludge flocs appeared, the connection among the flocs became closer, and the biofacies and number of microorganisms (Opercularia Coaractata, Nematode, Vorticella, etc.) increased significantly. HFP can be used as an excellent ECS for low carbon source wastewater, and probably also have the ability of slow-releasing carbon, and can be applied to municipal sewage or other wastewaters with insufficient carbon source.

Identification and characterization of differentially expressed miRNAs between bamboo shoot and rhizome shoot

Qun-Ying Jin,Hua-Zheng Peng,Er-Pei Lin,Nan Li,Dan-Ni Huang,Yan-Li Xu,Xi-Qi Hua,Kui-Hong Wang,Tang-Jun Zhu 한국식물학회 2016 Journal of Plant Biology Vol.59 No.4

As one of the largest members of Poaceae family, bamboo is a very important agricultural plant in the world. The development of bamboo shoot is very special and particularly significant to bamboo production. Understanding the developmental differences between bamboo shoot and rhizome shoot is extremely valuable for us to further elucidate the mechanism of bamboo shoot formation since both bamboo shoot and rhizome shoot develop directly from rhizome bud underground. In this paper, miRNA chips with 413 miRNA probes were used to compare miRNA expressions between bamboo shoot and rhizome shoot. The experiment revealed 64 bamboo shoot upregulated and 56 rhizome shoot up-regulated miRNAs which were classified into four major categories according to deep sequencing based target prediction. Meristem and morphological development related miRNAs were most important in bamboo shoot, especially miR171 and miR156 members. While in rhizome shoot the mainstream of miRNA expressions was metabolism and nutrition related ones, especially miR395 members. The meristem and morphological development related miRNAs in bamboo shoot showed some embryonic characteristics and suggested the participation of several phytohormones like gibberellin, cytokinin and auxin, which were absent in those miRNAs of rhizome shoot. Further qRT-PCR detections of 21 up-regulated miRNAs in bamboo seedlings indicated that 12 ones were regulated to varying degrees by some environmental factors. Among them, rhizome shoot upregulated osa-miR395b was the most environment-sensitive miRNA, particularly to dehydration. And the bamboo shoot up-regulated osa-miR399j proved uniquely and strongly induced by phosphor. The existence of multiple regulation sites from same miRNA suggested the probability of crosstalks among meristem development, metabolism and stress response during bamboo shoot and rhizome shoot development.

Elevated Mean Platelet Volume is Associated with Presence of Colon Cancer

Li, Jia-Ying,Li, Ying,Jiang, Zheng,Wang, Rui-Tao,Wang, Xi-Shan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: Colon cancer is the second most common cancer in developed countries. Activated platelets play a key role in inflammation and atherothrombosis, with mean platelet volume (MPV) is an early marker of platelet activation. The aim of the study was to clarify the relevance of MPV in patients with colon cancer. Materials and Methods: We measured MPV levels in 128 patients with colon cancer before and after surgery, and 128 controls matched for age, gender, body mass index (BMI) and smoking status. The odds ratios (ORs) and 95% confidence intervals (CIs) for colon cancer were calculated using multivariate logistic regression analyses across MPV quartiles. Results: Patients with colon cancer had higher MPV compared with controls. Surgical tumor resection resulted in a significant decrease in MPV levels (11.4 fL vs 10.7 fL; p<0.001). A positive correlation between MPV and tumor-nodule-metastases (TNM) stage was found. Furthermore, after adjusting for other risk factors, the ORs (95%CIs) for colon cancer according to MPV quartiles were 1.000, 2.238 (1.014-4.943), 3.410 (1.528-7.613), and 5.379 (2.372-12.198), respectively. Conclusions: The findings show that patients with colon cancer have higher MPV levels compared with controls, and these are reduced after surgery. In addition, MPV was found to be independently associated with the presence of colon cancer. Further studies are warranted to assess the utility of MPV as a novel diagnostic screening tool for colon cancer.

Comparative Analysis of the Genomes of Bombyx mandarina and Bombyx mori Nucleopolyhedroviruses

Yi-Peng Xu,Zheng-Pei Ye,Chang-Ying Niu,Yan-Yuan Bao,Wen-Bing Wang,Wei-De Shen,Chuan-Xi Zhang 한국미생물학회 2010 The journal of microbiology Vol.48 No.1

The Bombyx mandarina nucleopolyhedrovirus (BomaNPV) S1 strain can infect the silkworm, Bombyx mori,but is significantly less virulent than B. mori nucleopolyhedrovirus (BmNPV) T3 strain. The complete nucleotide sequence of the S1 strain of BomaNPV was determined and compared with the BmNPV T3 strain. The circular, double stranded DNA genome of the S1 strain was 126,770 nucleotides long (GenBank accession no. FJ882854), with a G+C content of 40.23%. The genome contained 133 potential ORFs. Most of the putative proteins were more than 96% identical to homologs in the BmNPV T3 strain, except for bro-a, lef-12,bro-c, and bro-d. Compared with the BmNPV T3 strain, however, this genome did not encode the bro-b and bro-e genes. In addition, hr1 lacked two repeat units, while hr2L, hr2R, hr3, hr4L, hr4R, and hr5 were similar to the corresponding hrs in the T3 strain. The sequence strongly suggested that BomaNPV and BmNPV are variants with each other, and supported the idea that baculovirus strain heterogeneity may often be caused by variation in the hrs and bro genes.

TiO2 thin films prepared from aqueous solution and their sterilizing capability

Sun Shu-qing,Hu Yan-tao,Xi Zheng-ying,Duan Chun-yan,Meng Yan 한양대학교 세라믹연구소 2006 Journal of Ceramic Processing Research Vol.7 No.1

A liquid phase deposition method (LPD) has been devised for the deposition of TiO2 thin films on glass at a low temperature. The substrates were immersed in a diluted homogeneous aqueous solution which was prepared by mixing equal volumes of ammonium hexauorotitanate and boric acid solution. The TiO2 thin films obtained were well-adhered, homogenous and colored by interference of reflected light. XRD indicated TiO2 thin films showed a high degree of crystallinity and the crystal phase observed was anatase. SEM experiments showed that the films are formed by small crystallites having linear dimensions under 50 nm. Their thickness was in the range of 100~300 nm. The sterilizing experiment of S. aureus and E. coli showed that TiO2 thin films have excellent sterilizing activity. The optimum conditions of the films were with heat treatment at 400oC where their sterilizing percentage reached over 99% after 24h.

Notes : PCR-Based Detection of Densovirus Infection in Silkworm (Bombyx mori L.)

( Cheng Xiang Hou ),( Mu Wang Li ),( Zhong Zheng Gui ),( An Ying Xu ),( Xi Jie Guo ) 한국잠사학회 2005 International Journal of Industrial Entomology Vol.11 No.2

Somatic Cell Nuclear Transfer of Oocytes Aspirated from Postovulatory Ovarian Follicles of Superovulated Rabbits

Shang, Jiang-Hua,Xu, Ru-Xiang,Jiang, Xiao-Dan,Zou, Yu-Xi,Qin, Ling-Sha,Cai, Ying-Qian,Yang, Zhi-Jun,Zheng, Xing,Cui, Sheng Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.9

The aim of this study was to evaluate if oocytes, aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration, could be efficiently used as ooplasm recipients for somatic cell nuclear transfer (SCNT). Within a common SCNT protocol, a comparison between oocytes recovered by direct aspiration (aspirated) from available ovarian follicles and oocytes flushed out from oviducts (flushed) was carried out. The results showed that maturation and enucleation rates of aspirated oocytes were 70.7% and 69.2%, significantly lower than 95.3% (p<0.01) and 83.6% (p<0.05), respectively, from flushed oocytes. However, following enucleation of matured oocytes as ooplasm recipients for SCNT, no difference was recorded in fusion and cleavage rates, as well as blastocyst development from cleaved embryos or hatching of blastocysts between aspirated and flushed groups. Additionally, some matured aspirated and flushed oocytes were also used for immediate parthenogenetic activation and the resulting embryo development was not significantly different. Results from this study show the following: i) the majority of oocytes aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration are matured and can be used directly as ooplasm recipients for SCNT; ii) the reconstructed embryos derived from these oocytes have similar in vitro developmental ability to those flushed from the oviducts.