산채류 추출물이 세균의 성장에 미치는 영향(Ⅰ)

지원대,이종만,정영건 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

본 실험은 일반 가정의 식탁에서 흔히 볼 수 있는 산채류 20종에 대해 3주의 gram 양성균(Bacillus subtilis, Lactobacillus casei, Staphylococcus aureus)및 2주의 gram음성균(Klebsie-lla pneumoniae, Shigella sp.)의 성장에 미치는 영향을 조사하였다. 본 실험에 사용한 대부분의 산채류 추출액은 공시균의 성장을 다소 증가시켰다. 특히 표고버섯과 고사리는 그람양성균인 B.subtilis, L.casei, S.aureus균의 취, 더덕과 토란은 B.subtilis, S. aureus균의 성장을 대조군에 비해 2~6배로 크게 증가시켰다. 그러나 그람음성균인 K.pneumoniae와 Shigella sp.에 대해서는 성장을 크게 증가시키는 시료는 없었다. 한편, 죽순은 B.subtilis의, 부지깽이와 민들레는 L.casei의, 팽이버섯과 돌나물 및 두릅은 S. aureus의, 산초, 다래순, 두릅 및 마는 K. pneumoniae의, 죽순, 두릅 및 마는 Shigella sp.의 생육을 다소 저해하는 것으로 나타났으나 항균력은 인정할 수 없었다. This study was carried out to investigate how to influence mountainvegetables, which are commonly available in high mountains in Korea, on bacte-rial growth. Total 20 kinds of mountain vegetables and mushrooms were sampledand after boiling them in water, put water extracts of them into the media prepa-red for the smear of test strains, which were included three of Gram positivestrains (Bacillus subtilis, Lactobacillus casei and Staphylococcus aureus) andtwo of Gram negative strains(Klebsiella pneumoniae and Shigella sp.). The gro-wth of all strains were more or less increased in the media injected with waterextracted samples than that of the control. Especially the growth of B. subtilis,L. casei and S. aureus were increased by the water extracts of Pyogo mushroomand Bracke and the samples from Aster, Deodeok(Codonopsis lanceolata) andTaro were appeared with the increase in growth of B, subtilis and S. aureusby two to six times increase rate than that of control. However, the more growthincrease of K. pneumoniae and Shigella sp. were not found than that of control.There were also observed some of water extracted samples have slight inhibi-tions to the bacterial growth. The growth of strains inhibited by the sampleswere as follows .B. subtilis by water extracts of Bamboo shoot, L. casei bythem of Busiggaengi(Erysimum aurantiacum) and Dandelion, S. aureus by themof Pengi mushroom, Sedum and Fatsia, K. pneumoniae by of them of Chinesepepper, Darae(Actinidia arguta) , Fatsia and Yam and Shigella sp. by of themof Bamboo shoot. Fatsia and Yam. However. these antibiotic effects were soweak that scarecely proved their effects.

분자유전학적방법에 의해 확진된 Leber씨 유전성 시신경병증 2예

김응규,김종환,김상진,예종수,김성은,허경,원인건,황정민 대한신경과학회 1998 대한신경과학회지 Vol.16 No.1

조혈모세포이식 환자에서 침습성 진균 감염에 대한 Micafungin의 예방 효과 및 안전성

김시현,이동건,최수미,권재철,박선희,최정현,유진홍,이성은,조병식,김유진,이석,김희제,민창기,조석구,김동욱,이종욱,민우성,박종원 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Background: Micafungin, a potent inhibitor of 1,3-β-D-glucan synthase, is a novel antifungal agent of the echinocandin class. In vitro study showed that micafungin was effective against Aspergillus species as well as Candida species, but clinical data on the prophylactic efficacy against invasive fungal infections (IFIs) other than candidiasis are still lacking. Materials and Methods: We identified 60 consecutive adult hematopoietic stem cell transplantation (HSCT) recipients who received at least 3 doses of micafungin during neutropenic period. Micafungin was started as an alternative in patients who were intolerant or had adverse events (AEs) to primary prophylactic antifungal agents. We retrospectively reviewed the medical records and analyzed the efficacy and safety of micafungin for prophylaxis against IFIs. Results: The patients either had autologous (n=9) or allogeneic (n=51: 1 syngeneic, 24 sibling, 26 unrelated donor) HSCT. Itraconazole oral solution (n=58) was the most frequently used first line antifungal agent for prophylaxis and was administered for median 11 days. The most frequent cause of switch to micafungin was vomiting (n=42). The duration of neutropenia and micafungin administration was median 13 and 12 days, respectively. A successful outcome was achieved in 45 (75%) patients. Empirical antifungal therapy was initiated in 13 (22%) patients. There were 2 cases (3.3%) of breakthrough fungal infections which comprised a probable invasive pulmonary aspergillosis and a possible invasive fungal sinusitis. There was no case of invasive candidiasis. A total of 53 (88%) patients experienced at least one AE regardless of causality during micafungin administration. The most frequent AEs were hypokalemia, vomiting, diarrhea, and elevated serum aspartate aminotransferase or alanine aminotransferase. Among the aforementioned AEs, only 1 case of diarrhea could be classified as a probable relation with micafungin when causality was assessed. There was no AEs that caused discontinuation of micafungin. Conclusions: Micafungin seems to be a safe and effective agent for prophylaxis of IFIs including aspergillosis as well as candidiasis in HSCT recipients. However, further large, prospective, and randomized comparative studies are warranted for aspergillosis.

再生骨材 콘크리트의 特性에 미치는 잔골재 混合의 影響에 關한 實驗的 硏究

李正熙,宋河永,文鐘健,趙明元 大田産業大學校 1997 한밭대학교 논문집 Vol.14 No.1

It is becoming increasing1y difficult and expensive for construction contractors not only to dispose of building waste and demolition rubble but also to obtain natural aggregates of good quality for concrete product and building construction. As one of solutions for the above-mentioned problems, this study was planned to suggest a fundamental data and method for producing recycled aggregate concrete using crushed cement bricks as fine aggregates. In this study, one normal concrete with natural river sand as fine aggregate and crushed stone as coarse aggregate and three recycled aggregate concretes according to the mix proportion of recycled fine aggregate substitution ratio of 35%, 65%, 100% were made using tile water-cement ratio of 45% and 65%.

Staphylococcus aureus와 Coagulase-Negative Staphylococcus Species에 대한 Arbekacin의 시험관내 항균력

위성헌,강진한,허동호,이동건,김상일,김양리,최정현,김종현,유진흥,허재균,신완식,강문원 대한감염학회 2001 감염 Vol.33 No.4

Background : Most strains of methicillin-resistant Staphylococcus aureus (MRSA) now exhibit high-level resistance to various antibiotics, such as β -lactam antibiotics, aminoglycosides, macrolides, tetracyclines and quinolones. Recent reports describing the therapeutic failure of vancomycin for MRSA infections have arisen considerable concerns regarding the emergence of MRSA strains, which will require new therapeutic agents. Arbekacin, an aminoglycoside antibiotic, has antibacterial activity against both gram-positive and gram-negative bacteria and is stable in the presence of aminoglycoside inactivating enzymes produced by S. aureus. In this study, we compared the antibacterial activity of arbekacin with those of vancomycin, gentamicin, and amikacin against Staphylococcus aureus (S. aureus) and coagulase-negative staphylococci (CNS). Methods : For a collection of 549 S. aureus and 251 CNS isolates from three Catholic University Hospitals in Korea, minimum inhibitory concentrations (MICs) of arbekacin, vancomycin, amikacin and gentamicin were determined by agar dilution method using Mueller-Hinton agar according to NCCLS (National Committee for Clinical Laboratory Standards, USA)criteria. Results : Among 549 S. aureus isolates, 278 isolates were MRSA and 271 isolates were methicil sensitive S. aureus (MSSA). MIC50 & MIC90 of arbekacin against 549 S. aureus were 0.5 & 1 ㎍/mL, and MIC50 & MIC90 of vancomycin were 1 & 1 ㎍/mL. MIC of arbekacin against 549 S. aureus isolates ranges from 0.03 to 4 ㎍/mL, and MIC of vancomycin against 549 S. aureus ranges from 0.25 to 2 ㎍/mL. MIC90 of amikacin against 549 S. aureus was 32㎍/mL, and that of gentamicin was 128 ㎍/mL. MICs of amikacin and gentamicin were variable, ranging from 0.125 to 256, and otherwise arbekacin and vancomycin revealed relatively narrow range of MICs. MIC90 of arbekacin against 278 MRSA isolates & 271 MSSA were 1 & 0.5 ㎍/mL, and those of vancomycin against MRSA & MSSA were 1 & 1 ㎍/mL. MIC90 of amikacin against 278 MRSA & 271 MSSA isolates were 32 & 4 ㎍/mL, and that of gentamicin against MRSA & MSSA isolates were 128 & 32 ㎍/mL respectively. Among 251 CNS isolates, 122 isolates were MRCNS and 129 were MSCNS. MICSO & MIC90 of arbekacin against 251 CNS isolates were 0.25 & 2 ㎍/mL, and those of vancomycin were 1 & 2 ㎍/mL. MIC of arbekacin against 251 CNS isolates ranges from 0.015 to 32 ㎍/mL, and that of vancomycin isolates ranges from 0.25 to 2 ㎍/mL, MIC90 of arbekacin against 122 MRCNS & 129 MSCNS isolates were 2&0.3 ㎍/ML, and those of vancomycin were 2&s ㎍/ML. MIC90 of amikacin against 251 CNS isolates was 32 ㎍/ML, and that of gentamicin was 128 ㎍/ML for CNS. MIC90 of amikacin against 122 MRCNS & 129 MSCNS isolates were 128 & 8㎍/mL, and those of gentamicin ere 256 & 32 ㎍/mL. Conclusion : Considering above results, arbekacin can be useful agent against most strains of MRSA and MRCMS, which exhibit high-level resistance to amikacin and gentamicin. (Korea J Infect Dis 33:254~260, 2001)

판막 질환에서의 심근 섬유화 : 심근 조직을 이용한 형태학적 연구 및 혈청 생화학적 지표의 분석

강수진,박덕우,송재관,박경민,송종민,강덕현,한기훈,송현,이재원,주석중,송명근,김정선 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

Response of Rice Yield and Grain Quality by Rice Leaf Folder Injury

Jong Gun Won,Duok Jong Ahn,Se Jong Kim,So Deuk Park,Sang Chul Lee 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.39 No.-

Relationship Between Negative Geotropism and Seedling Traits on Rice

Won Jong-Gun,Ahn Deok-Jong,Park Sang-Gu,Park So-Deuk 한국작물학회 2005 한국작물학회 학술발표대회 논문집 Vol.2005 No.-

Morphological Difference of Rice Seedling Grown under Different Dissolved Oxygen Conditions

Won Jong Gun,Choi Jang Soo,Ahn Deck Jong,Lee Seung Phil,Lee Sang Chul,Yoshida Tomohiko The Korean Society of Crop Science 2004 Korean journal of crop science Vol.49 No.4

The response of dissolved oxygen (DO) concentrations caused significant change in root number, root length, coleoptile length, shoot length and leaf age of seedlings. The genotypic difference in the effect of DO also highly significant (P<0.01) for all of the seedling traits. The number and length of root were extremely inhibited at the condition of $0.39\pm0.09$ DO concentration. While the coleoptile elongated markedly in the lowest DO concentrations, the shoot did not develop. The root growth was improved slightly at the $1.39\pm0.27mg L^{-1}$, however, there were no difference among genotypes at these two low DO concentrations. As the DO concentration become higher, the growth of root and shoot was improved remarkably. Root number, root length and shoot length was significantly different between $20\;and\;30^{\circ}C$ in DO rich and normal conditions, the development of those traits were apparently accelerated in high water temperature, however those traits of seedlings in DO deficiency were not different between the two temperatures except for shoot length. On the other hand the coleoptile length was not affected by the stagnant water temperature; it was stimulated by the low DO concentration. The competition of DO was greater as the seedling density was increased in the stagnant water, therefore the seedlings grown under high density have long and white coleoptiles, and the growth of roots and shoots was retarded severely.

Competitiveness and Yield Loss Prediction of Red Pepper by Upland Weeds

Jong-Gun Won,Kil-Su Jang,Ji-Eun Hwang,Oh-Hun Kwon,Su-Gyung Jeon,Tae-Young Kwon 한국잡초학회 2011 한국잡초학회 별책(학술대회 초록집) Vol.31 No.2