위장관의 원발성 B 세포 악성 림프종의 재분류와 bcl-2, cyclin D1, bcl-6 및 p53 발현의 의미

서정균,김태원,김병수,조은택,박찬국,김만우,이미자,전호종 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : In recent years, the REAL (Revised European American Lymphoma) classification provided a new category of MALT (mucosa-associated lymphoid tissue) lymphoma and mantle cell lymphoma in B cell lymphomas. We have analyzed the expression of bcl-2, cyclin D1, bcl-6 and p53 and correlated with the subtypes and histologic grade of primary gastrointestinal B cell lymphoma. Also we investigated the usefulness of immunophenotypic features in diagnosis of low grade B cell lymphoma. Materials and Methods : Twenty-two cases of primary gastrointestinal B cell lymphoma were recategorized in low grade MALT lymphoma, low/high grade MALT lymphoma, high grade MALT lymphoma, and diffuse large cell lymphoma according to the morphological findings. We investigated the expression of bcl-2, cyclin D1, bcl-6 and p53 by immunohistochemical method. Results : The bcl-2 protein expression was higher in the low grade MALT lymphoma than in the high grade lymphoma. The cyclin D1 protein expression was negative in all cases. The expression of bcl-6 and p53 protein was negative in all low grade MALT lymphoma. Conclusion : The results suggest that we can differentiate the low grade lymphoma from the high grade lymphoma by immunohistochemical staining for bcl-2, bcl-6, and p53 protien.

Diels-Alder 반응을 통한 Cycloadducts of Bicyclolactone의 입체 이성질체 구조 연구

서찬우,원호식 한양대학교 이학기술연구소 2005 이학기술연구지 Vol.8 No.-

3.3-dibromo-2-pyrone의 Diels-Alder cycloaddition 으로 부터 얻은 Bicyclokactone은 다양 palladium 촉매 하에서 aryclolaction으로 반응 시킬 수 있다, N-Et maleimide(NEM)를 사용한 Bromo-cycloaddition으로 endo-fro, (38%)의 두개의 다른 입체 이성질체를 생성하고 두 물질은 NMR에 의해 분석된다. 이연구 에서 COSY,TOCSY, NOESY, ROESY를 이용하여 각각의 isomer에 대한 NMR 시그널을 지정하였다, 여러 종류의 수소의 거리는 NOE cross peak를 이용하여 얻을수 있었다, 이 distance data를 기반으로 distace geometry(DG)와 molecular dynamics(MD)를 이용하여 두개의 endo-form,exo-form isomer들의 구조를 결정하였다. Bicyclactones obtained from the Diels-Alder cycloaddition of 3,5-dibromo-2-pyrone can be changed into aryl bicyclolactones by cross coupling reactions under various palladium catalyst Bromo-bicyclic diene furnished two different diastereomers endo-form(62%)and exo-from(38%) upon cycloadditions with N-Et maleimide (NEM) and their stereochemistries were identified with NMR In this study The NMR signal assignments of each isomer was completely accomplished with COSY, TOCSY, NOESY and ROESY Distance of numerous protons were obtained based on the NOE cross peak intensities of NOESY spectrum. On the basis of these distance data distance geometry(DG) and molecular dynamics(MD) were carried out to determine the isomer structures of endo-from and exo-from

간경변 환자에서 Oro-cecal Transit Time의 측정

원경준,이준,변유미,조민근,조주연,서정균,김영대,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

Background: Neurotransmitter like substances in the gut have been proposed to contribute to the overall portal systemic encephalopathy (PSE). The source of the ammonia in patients with PSE is mainly the small bowel, as a result of the conversion of glutamine in the diet to glutamate and ammonia. PSE is highly responsive to changes in diet, to antibiotic therapy and to ingestion of nondigestible disaccharides such as lactulose. This study was performed to evaluate the relationship between PSE and oro-cecal transit time. In addition, performed to evaluate the effect of severity, cause, complications of liver cirrhosis on oro-cecal transit time. Methods: Between August 2006 and February 2007, 48 cirrhotic patients consecutively was enrolled in study their oro-cecal transit time after informed consent was obtained. A non-invasive hydrogen breath test was used to study oro-cecal transit time (OCTT). Result: The OCTT were delayed in cirrhotic patients with PSE compared without PSE (123.3 ± 42.7 min vs 170.0 ± 58.3 min, p<0.05). OCTT tended to prolongate further as the damage in the liver got worse, nevertheless the result was statistically meaningless (p<0.111). Neither presence of ascites or cause of PSE had any influence on gastrointestinal transit. Conclusions: Cirrhotic patients with PSE have more delayed OCTT than without PSE. Further research is needed to find out the relationship between OCTT and severity of liver cirrhosis.

다양한 분광법을 이용한 Calcium-Alizarin Red S 착물의 연구

서찬우,원호식 한양대학교 이학기술연구소 2003 이학기술연구지 Vol.6 No.-

섬유의 염색에 주로 이용되었던 염료는 과학기술의 발달과 함께 점차 그 용도와 종류가 다양해져 왔다. 그 중 안드라퀴논 계 염료 중 하나인 Alizarin Red S는 calcium mineral과 선택적으로 반응할 수 있는 염료로 현미경 관찰을 위한 세포 염색과 관련된 연구가 다양하게 진행되고 있다. Alizarin Red S 와 calcium의 complex를 합성하기 위해 calcium nitrate와 Alizarin Red S를 반응시켰으며 물리화학적인 변화와 calcium의 결합위치에 대한 정보를 얻기 위해 UV/Visible, FT-IR,NMR을 통한 구조변화를 분석해보았다. λ_(max)=505nm에서의 강한 흡수밴드, 1680∼1720cm^(-1)에서의 C=0의 신축진동의 변화 그리고 C4(△σ=1.196, C6(△σ=1.259)의 ^(13)C-NMR 화학적 이동 변화가 관측되었으며 이 결과는 Alzarin Red S의 calcium 결합에 carbonyl 작용기와 인접 hydroxy작용기가 관여한다는 것을 나타낸다. Usages and chemical species of dying reagents formerly only used for the texile industry have becomer so diverse as development of science technology. Alizarin Red S, one of anthraquinone dying reagents is widely utilized in cell staining for microscopic observation due to the calcium binding selectivity. The molecular structure and physicochemical properties of calcium-Alizarin Red Scomplex were characterized by UV/Vis, FT-IR, FT-NMR studies. The characteristic absorption band at λ_(max)=505nm, the change of C=0 stretching vibration at 1680∼1720cm^(-1), and ^(13)C-NMR chemical shift changes of C4(△σ=1.196), C6(△σ=1.259) indicated that the calcium bidning site of Alizarin Red S are the hydroxyl group and carbonyl oxygen, respectively

근관 세척법이 Resilon/Epiphany sealer와 Gutta-percha/AH 26 sealer의 근관 상아질과의 접착에 미치는 영향

김서경,황윤찬,황인남,오원만 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.2

본 연구에서는 근관 세척법이 두 가지 근관 충전재 (Gutta-percha/AH 26 sealer, Resilon/Epiphany sealer)의 근관 상아질과의 접착메 미치는 영향을 평가하였다. 총 30개의 발거된 상악 전치를 각자 5개씩 6개의 군으로 분류하여 근관 성형을 하고 마지막 세척을 다음과 같이 시행 한 후 Gl, G2, G3군은 Gutta-percha/AH 26 sealer로, Rl, R2, R3군은 Resilon/Epiphany sealer로 근관 충전하였다. Gl, Rl군 : 5.25% NaOCl세척 G2, R2군 : 5.25% NaOCl세척 후 sterile saline 세척 G3, R3군 : 5.25% NaOCl세척 후 17% EDTA, sterile saline 순으로 세척 각 군의 충전된 치아를 아크릴 레진에 매몰하고 약 1 mm 두께로 절단한 후 만능 물성 시험기상에서 push-out bond strength를 측정하였고 실험 후 파절 양상을 Image-analyzing microscope으로 관찰하여 다음과 같을 결과를 얻었다. 1. Gutta-percha/AH 26 sealer 충전군에서 Resilon/Epiphany sealer 충전군보다 push-out bond strength가 높게 나타났다 (p < 0.05). 2. Resilon/Epiphany sealer 충전군 중 17% EDTA, sterile saline순으로 세척시 5.25% NaOCl 세척에 비해 push-out bond strength가 높게 나타났다 (p<0.05). 3.파절 양상을 분석시 모든 군은 접착성과 혼합성 파절 양상을 나타내었고, Gl, G2, Rl 군은 응집성 파절 양상이 나타나지 않았으며, Resilon/Epiphany sealer 충전군은 Gutta-percha/AH 26 sealer 충전군에 비해 응집성과 혼합성 파절 망상이 더 높은 비율로 나타났다. The purpose of this study was to evaluate whether intracanal irrigation method could affect the adhesion between intracanal dentin and root canal filling materials (Gutta-percha/AH 26 sealer and Resilon/Epiphany sealer). Thirty extracted human incisor teeth were prepared. Canals were irrigated with three different irrigation methods as a final rinse and obturated with two different canal filling materials (G groups : Gutta-percha/AH 26 sealer, R groups : Resilon/Epiphany sealer) respectively. Group Gl, Rl - irrigated with 5.25% NaOCl Group G2, R2 - irrigated with 5.25% NaOCl, sterile saline Group G3, R3 - irrigated with 5.25% NaOCl, 17% EDTA, stehle saline Thirty obturated roots were horizontally sliced and push-out bond strength test was performed in the universal testing machine. After test, the failure patterns of the specimens were observed using Image-analyzing microscope. The results were as follows. 1. Gutta-percha/AH 26 sealer groups had significantly higher push-out bond strength compared with the Resilon/Epiphany sealer groups (p < 0.05). 2. Push-out bond strength was higher when using 17% EDTA followed by sterile saline than using NaOCl as a final irrigation solution in the Resilon/Epiphany sealer groups (p < 0.05). 3. In the failure pattern analysis, there was no cohesive failure in Group Gl, G2, and Rl. Gutta-percha/AH 26 sealer groups appeared to exhibit predominantly adhesive and mixed failure patterns, whereas Resilon/Epiphany sealer groups exhibited mixed failures with the cohesive failure occurred within the Resilon substrate.

Aspergillus awamori와 Zymomonas mobilis로 구성된 고정화혼합 배양계의 에탄올 생산에 미치는 Triton, PVA 및 PEG의 영향

이상원,서권일,박석규,손봉수,김홍출,성찬기 순천대학교 기초과학연구소 1995 基礎科學硏究誌 Vol.6 No.-

The effects of triton-X 100, PVA and PEG on the ethanol production and raw starch hydrolysis by co-immobilized A. awamori and Z. mobilis (A-Z system) were investigated. When gel beads containing A. awamori(1.25 × 10 exp (9) spores/ℓ - gel) and Z. mobilis(0.5g cell/ℓ - gel) were cultured in 2% raw starch solution at 30℃ for 120 hours, ethanol concentration, ethanol yield (Y_p/s) and pH of culture broth were 2.8g/ℓ, 0.16 and 3.8~4.0, respectively. Ethanol production and starch hydrolysis by addition of PVA was efficient, compared to that of PEG and ethanol concentration in culture broth with 0.2% PVA was 3.56g/ℓ. At A-Z 36 culture system with changing silicon check valve for cotton plug at 36 hours in A-Z culture system, ethanol yield on glucose, Y_p/s = 0.36, showed 2 times higher than that of control culture system(cotton plug culture). Ethanol production and productivity by cultivating 85g gel beads at 30℃ in a 1ℓ jar fermentor(220rpm) of medium 500㎖ were 32g/ℓ and 1.8g/ℓ/day, respectively. After 12 hours of cultivation, gel beads were distinguished oxygen-rich surface for A. awamori from oxygen-deficient central part for Z. mobilis.

근관 전색재의 방사선 불투과성에 관한 비교연구

김태민,김서경,황인남,황윤찬,강병철,윤숙자,이재서,오원만 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.1

This study was performed to assess the radiopacity of a variety of root canal sealers according to the Specification concerning root canal sealers. Ten materials including Tubli-Seal™, Kerr Pulp Canal Sealer™, AH 26^(R), AH plus^(R), AH plus jet™, Ad seal™, Sealapex™, NOGENOU™, ZOB seal™, Epiphany™, and dentin were evaluated in this study. In the first part. densitometric reading of an each step of aluminum step wedge on occlusal film was performed at different voltage and exposure time. In the second part, ten specimens were radiographed simultaneously with an aluminum step wedges on the occlusal films under decided condition. The mean radiographic density values of the materials were transformed into radiopacity expressed equivalent thickness of aluminum (mm Al). The following results were obtained 1 Among the various conditions, the appropriate voltage and exposure time that meet the requirement density was 60 kVp at 0.2 s 2 All of the materials had greater radiopacity than 3 mm Al requirement of ANSI/ADA specification No. 57 (2000) and ISO No. 6876 (2001) standards. 3 The radiopacity of materials increased as thickness of materials increased. 4 The mm Al value of each specimen at 1mm in thickness has a significant difference in the statistics. It suggests that root canal sealers have a sufficient radiopacity that meet the requirement. 다양한 근관전색제는 방사선 사진상 주위 해부학적 구조와 구별될 만한 방사선 불투과성을 나타내야 한다 따라서 이런 물질들이 근관에 충전될 때의 방사선 불투과성 정도를 평가해야 할 필요성이 대두되고 있다. 본 실험에서는 다양한 근관전색제들의 방사선 불투과성을 평가하고자, 방사선 노출조건에 따른 aluminium step wedge에 대한 광학 밀도를 알아보고, 그 중 적절한 노출조건을 선택하여 수종의 근관 전색재의 방사선 불투과성 정도를 알루미늄 두께로 환산하여 비교해 보고자 한다. 방사선 불투과성의 기준을 위해 11개의 step으로 구성된 aluminum step wedge을 사용하여, 60kVP, 70kVp관전압 상태에서 각각 0.2, 0.3, 0.4초 그리고 0.2, 0.3, 0.33초의 꼭 노출시간으로 교합필름상에서 방사선 촬영후 적절한 노출 조건을 구하였다. 직경 5mm 각각의 두께 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mm인 10종 (Tubli-Seal™, Kerr pulp Canal Sealer™, AH26^(R), AHplus^(R), AH plus jet starter kit™, Ad seal™, Sealapex™, Nogenol root canal sealer™, ZOB seal™, Epiphany™)의 근관전색재 시편을 각 재료와 두께당 10개씩 제작한 후, 동일한 두께의 상아질 시편, aluminum step wedge와 함께 정해진 노출시간에 따라 방사선 촬영을 하였다. 모든 필름은 자동현상기로 현상하였다. 시편의 방사선 흑화도를 densitometer로 5회 반복 측정 후, 평균값을 구하여 알루미눔 두께로 환산하였다.얻어진 정보를 분석하여 다음의 결과를 얻었다. 1 관전압 60 kVp에서 노출시간 0.2, 0.3, 0.4초, 70 kVp에서 0.2, 0.3, 0.33초로 변화를 주어 방사선 촬영을 하였을 때 흑화도가 ISO No. 6876 규격에 가장 적합한 것은 60 kVP, 0.2초 일 경우였다. 2 측정된 근관 전색제의 방사선 불투과성은 2.29 mm Al (N0GEN0L)로부터 13.69 mm Al (AH Plus jet)까지 다양하게 나타났으나, 모두 ANSI/ADA specification (2000) 또는 ISO No. 6876 (2001) 규격이 제시한 최소한 3mm Al 이상의 방사선 불투과성을 지녀야 한다는 기준에 적합하였다. 3 재료의 두께가 증가할수록 방사선 불투과성은 증가하지만, 정비례하지는 않았다. 4 각 실험재료의 1 mm 두께의 시편에 대한 mm Al값들은 통계적으로 유의한 차이가 있었다. 이상의 결과는 본 실험에 사용된 수종의 근관 전색재는 모든 규격에 적합한 방사선 불투과성을 가지고 있음을 시사한다.

근관 충전재 및 치근단 역충전재의 방사선 불투과성에 관한 비교 연구

김용상,김서경,황윤찬,황인남,오원만 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.2

This study was performed to assess the radiopacity of a variety of canal filling and retrograde root-end filling materials according to the specification concerning root canal obturation materials. Ten materials including Gutta-percha pellets, amalgam, Fuji II LC, Dyract^(ⓡ) AP, Super EBA^(ⓡ), IRM^(ⓡ) AH 26^(ⓡ), Sealapex™, Tubli-Seal™ and dentin were evaluated in this study In the first part, densitometric reading of an each step of aluminum step wedge on occlusal film were performed at 60 kVp (0.2, 0.3, 0.4 s), 70 kVp (0.2, 0.3, 0.33 s) to decide appropriate voltage and exposure time. In the second part, ten specimens which are 5 ㎜ in diameter and 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 ㎜ in thickness, were fabricated from each material studied. The specimens were radiographed simultaneously with an aluminum step wedge under decided condition (60 kVp, 0.2 s). The mean radiographic density values of the materials were transformed into radiopacity expressed equivalent thickness of aluminum (㎜ Al). The following results were obtained. 1. Among the various conditions including 0.2 s, 0.3 s, 0.4 s at 60 kVp and 0.2 s, 0.3 s, 0.33 s at 70 kVp, the appropriate voltage and exposure time that meet the requirement of density from 0.5 to 2.0 was 0.2 s at 60 kVp. 2. All of the materials in this study had greater radiopacity than the minimun level recommended by ISO No. 4049 standards. 3. Most of the materials had greater radiopacity than 3 ㎜ Al requirement of ANSI/ADA specification No. 57 (2000) and ISO No. 6876 (2001) standards except for Fuji II LC and Dyract. It suggests that all experimental canal filling and retrograde root-end filling materials have a Sufficient radiopacity that meet the requirement concerning root canal obturation materials except for Fuji II LC and Dyract. 본 실험에서는 다양한 방사선 노출 조건에 따른 aluminum step wedge에 대한 광학 밀도를 알아보고, 그 중 적절한 노출조건을 선택하여 수종의 근관 충전재와 치근단 역충전재의 방사선 불투과성 정도를 굉가, 비교하였다. 방사선 불투과성의 기준을 위해 11개의 step으로 구성된 aluminum step wedge를 제작하여 , 60 kVp, 70 kVp 관전압 상태에서 각각 0.2. 0.3, 0.4초와 0.2, 0.3, 0.33초의 노출시간으로 교합 필름상에서 방사선 촬영 후 적절한 노출 조건을 구하였다. 직경 5 ㎜,각각의 두께 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 ㎜인 10종 (Gutta-percha, amalgam, Super EBA^(ⓡ), MTA, IRM^(ⓡ), Fuji II LC, Dyract^(ⓡ) AP, AH 26^(ⓡ), Sealapex^(ⓡ), Tubli-Sea™의 근관 충전재와 치근단 역충전재 시편을 각 재료와 두께당 10개씩 제작한 후, 동일한 두께의 상아질 시편 , aluminum step wedge와 함께 정해진 노출조건에 따라 방사선 촬영을 하였다. 모든 필름은 자동현상기로 현상하였다. 시편의 흑화도를 densitometer (Model 07-443, Victoreen Inc, Cleveland, Ohio, USA)로 5회 반복 측정 후, 평균값을 구하여 회귀분석 후 알루미늄 두께로 환산하였다. 얻어진 정보를 분석하여 다음의 결과를 얻었다. 1. 관전압 60 kVp에서 노출시간 0.2, 0.3, 0.4초, 70 kVp에서 0.2, 0.3, 0.33초로 변화를 주어 방사선 촬영을 하였을 때, 흑화도가 0.5 - 2.5 사이여야 한다는 ISO No. 6876 규격에 가장 적합한 것은 60 kVp, 0.2초일 경우였다. 2.측정된 근관 충전재와 치근단 역충전재들의 방사선 불투과성 모두 ISO No. 4049 규격에 적합하였다. 3.광중합형 글래스 아이오노머 (Fuji II LC)와 컴포머 (Dyract)를 제외하고는 ANSI/ADA specification (2000) 또는 ISO No. 6876 (2001)규격이 제시한 최소한 3 ㎜ Al 이상의 방사선 불투과성을 지녀야 한다는 규격에 적합하였다. 이상의 결과는 본 실험에 사용된 수종의 근관 충전재와 치근단 역충전재 중 Fuji Ⅱ LC와 Dyract를 제외하고 모두 규격에 적합한 방사선 불투과성을 가지고 있음을 시사한다.

원발성 후복막 점액낭샘암종 1예

지준호,이화정,박승찬,박정철,최은정,서혜진,이원식,이정림,배병조,손경락,이경희 영남대학교 의과대학 2008 Yeungnam University Journal of Medicine Vol.25 No.2

Primary retroperitoneal mucinous cystadenocarcinoma is a very rare malignancy, and little is known concerning its Pathogenesis, optimal treatment, and prognosis. A 29-year-o1d pregnant woman (21 weeks) Presented with abdominal discomfort CA 19-9, CA 125, and CEA were normal Abdominal CT scanning revealed a 19x15x13 cm retropentoneal tumor Exploratory laparotomy and tumor excision were performed. Mucinous retropentoneal implants were removed as completely as possible Histologically, the tumor showed focal areas of capsular invasion, but free resection margins The uterus and both ovaries were normal in appearance No adjuvant therapy was pursued. Six months later, Peritoneal and bilateral ovarian metastases were discovered. Hence, we report the details of this case of primary retroperitoneal mucinous Cystadeno-carcinoma and present a review of the literature.

췌장 선방세포암 1예

이화정,지준호,박승찬,박정철,최은정,서혜진,이원식,이정림,배병조,손경락,이경희 영남대학교 의과대학 2008 Yeungnam University Journal of Medicine Vol.25 No.2

Acinar cell carcinoma is a rare tumor that represents 1~2% of al1 pancreatic cancers. Clinical and radiologic findings are inconclusive in this disease Acinar cell carcinoma is characterized by rapid progression and early metastasis, which lead to its poor prognosis. A 41-year-o1d man was admitted to our hospital for abdominal pain. Abdominal computed tomography (CT) and positron emission tomography-computed tomography (PET-CT) showed a splenic mass, which was being invaded by a pancreatic tail mass and which had increased ^(18)F-fluorodeoxyglucose (FDG) uptake Primary radical distal pancreatectomy and splenectomy were performed. Pathologic findings revealed an acinar cell carcinoma of the Pancreas The patient underwent a total gastrectomy three months later because of gastric recurrence Four months later, multiple hepatic metastases were discovered, and the patient underwent a left hepatectomy During treatment with capecitabine, there was no evidence of tumor progression for 14 months. We report a case of metastatic pancreatic acinar cell carcinoma, which did not progress for an extended period while the patient was being treated with capecitabme.