The Effects of Languaging in Response to Automated Written Correction for Grammar Accuracy Improvement

Wenwen Xu,김지현 한국영어교육학회 2023 ENGLISH TEACHING(영어교육) Vol.78 No.1

This study explored the role of written languaging (WL) in response to automated written corrective feedback (AWCF) in L2 accuracy improvement in English classrooms at a university in China. A total of 254 freshmen enrolled in intermediate composition classes participated, and they wrote 4 essays and received AWCF. A half of them engaged in WL after receiving correction, while the other half did not. To measure whether WL could contribute to L2 accuracy improvement, error correction tests were conducted three times. AWCF targeted all types of errors, but the study focused on investigating six language features (nouns, conjunctions, subject-verb agreement, sentence constituents, articles, and prepositions) known as the most frequent errors intermediate students make. The results proved that WL had a positive effect on accuracy improvement overall. However, when individual language features were considered separately, the effects were not the same. The inherent complexity of the features along with L1 influence appeared to affect the effects of WL for accuracy improvement.

Association of the Porcine Cluster of Differentiation 4 Gene with T Lymphocyte Subpopulations and Its Expression in Immune Tissues

Xu, Jingen,Liu, Yang,Fu, Weixuan,Wang, Jiying,Wang, Wenwen,Wang, Haifei,Liu, Jianfeng,Ding, Xiangdong,Zhang, Qin Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.4

Cluster of differentiation 4 (CD4) is mainly expressed on $CD4^+$ T cells, which plays an important role in immune response. The aim of this study was to detect the association between polymorphisms of the CD4 gene and T lymphocyte subpopulations in pigs, and to investigate the effects of genetic variation on the CD4 gene expression level in immune tissues. Five missense mutations in the CD4 gene were identified using DNA pooling sequencing assays, and two main haplotypes (CCTCC and AGCTG) in strong linkage disequilibrium (with frequencies of 50.26% and 46.34%, respectively) were detected in the population of Large White pigs. Our results indicated that the five SNPs and the two haplotypes were significantly associated with the proportions of $CD4^-CD8^-$, $CD4^+CD8^+$, $CD4^+CD8^-$, $CD4^+$ and $CD4^+/CD8^+$ in peripheral blood (p<0.05). Gene expression analysis showed the mRNA level of the CD4 gene in thymus was significantly higher than that in lymph node and spleen (p<0.05). However, no significant difference was observed between animals with CCTCC/CCTCC genotype and animals with AGCTG/AGCTG genotype in the three immune tissues (p>0.05). These results indicate that the CD4 gene may influence T lymphocyte subpopulations and can be considered as a candidate gene affecting immunity in pigs.

Two-sex life table of Nasonia vitripennis (Hymenoptera: Pteromalidae) and the effects of Wolbachia on its reproduction and parasitism of Musca domestica (Diptera: Muscidae)

Yang Wenwen,Chu Xu,Li Jie,Wang Ningxin 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae) is now emerging as genetic model organism for development and genetics research. As a parasitic insect, the egg, larval, pupal, and early adult developmental stages of N. vitripennis occur within the enclosed fly pupae, which differ a lot from the life cycle of other insects that undergo complete metamorphosis. Previous report on the life table of N. vitripennis was based on females only. In this study, the two-sex life table approach was used to examine the parasitic efficiency of N. vitripennis within the pupae of Musca domestica L. (Diptera: Muscidae), and the influence of low temperature and the endosymbiotic bacteria Wolbachia on wasp population growth were investigated. Wolbachia could improve the fecundity of N. vitripennis and prolong the host life history. We propose the preservation of M. domestica pupae at 4 ◦ C for 15 days is suitable in practical use. Age-stage, two-sex life table analysis revealed the stage structure and variability of N. vitripennis population growth, and also provide useful information about the effects of Wolbachia on its reproduction and parasitism of M. domestica.

miR-458b-5p regulates ovarian granulosa cells proliferation through Wnt/β‐catenin signaling pathway by targeting catenin beta-1

Wang Wenwen,Teng Jun,Han Xu,Zhang Shen,Zhang Qin,Tang Hui 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.6

Objective: Ovarian follicular development, which dependent on the proliferation and differentiation of granulosa cells (GCs), is a complex biological process in which miRNA plays an important role. Our previous study showed that miR-458b-5p is associated with ovarian follicular development in chicken. The detailed function and molecular mechanism of miR-458b-5p in GCs is unclear. Methods: The luciferase reporter assay was used to verify the targeting relationship between miR-458b-5p and catenin beta-1 (CTNNB1), which is an important transcriptional regulatory factor of the Wnt/β-catenin pathway. The cell counting kit-8 (CCK-8) assay, flow cytometry with propidium iodide (PI) and annexin V-fluorescein isothiocyanate (FITC) labeling were applied to explore the effect of miR-458b-5p on proliferation, cell cycle and apoptosis of chicken GCs. Quantitative real-time polymerase chain reaction and Western blot were used to detect the mRNA and protein expression levels. Results: We demonstrated that the expression of miR-458b-5p and CTNNB1 showed the opposite relationship in GCs and theca cells of hierarchical follicles. The luciferase reporter assay confirmed that CTNNB1 is the direct target of miR-458b-5p. Using CCK-8 assay and flow cytometry with PI and Annexin V-FITC labeling, we observed that transfection with the miR-458b-5p mimics significantly reduced proliferation and has no effects on apoptosis of chicken GCs. In addition, miR-458b-5p decreased the mRNA and protein expression of CD44 molecule and matrix metallopeptidase 7, which are the downstream effectors of CTNNB1 in Wnt/β-Catenin pathway and play functional roles in cell proliferation. Conclusion: Taken together, the data indicate that miR-458b-5p regulates ovarian GCs proliferation through Wnt/β-catenin signaling pathway by targeting CTNNB1, suggesting that miR-458b-5p and its target gene CTNNB1 may potentially play a role in chicken ovarian follicular development. Objective: Ovarian follicular development, which dependent on the proliferation and differentiation of granulosa cells (GCs), is a complex biological process in which miRNA plays an important role. Our previous study showed that miR-458b-5p is associated with ovarian follicular development in chicken. The detailed function and molecular mechanism of miR-458b-5p in GCs is unclear.Methods: The luciferase reporter assay was used to verify the targeting relationship between miR-458b-5p and catenin beta-1 (<i><i>CTNNB1</i></i>), which is an important transcriptional regulatory factor of the Wnt/β-catenin pathway. The cell counting kit-8 (CCK-8) assay, flow cytometry with propidium iodide (PI) and annexin V-fluorescein isothiocyanate (FITC) labeling were applied to explore the effect of miR-458b-5p on proliferation, cell cycle and apoptosis of chicken GCs. Quantitative real-time polymerase chain reaction and Western blot were used to detect the mRNA and protein expression levels.Results: We demonstrated that the expression of miR-458b-5p and <i>CTNNB1</i> showed the opposite relationship in GCs and theca cells of hierarchical follicles. The luciferase reporter assay confirmed that <i>CTNNB1</i> is the direct target of miR-458b-5p. Using CCK-8 assay and flow cytometry with PI and Annexin V-FITC labeling, we observed that transfection with the miR-458b-5p mimics significantly reduced proliferation and has no effects on apoptosis of chicken GCs. In addition, miR-458b-5p decreased the mRNA and protein expression of CD44 molecule and matrix metallopeptidase 7, which are the downstream effectors of <i>CTNNB1</i> in Wnt/β-Catenin pathway and play functional roles in cell proliferation.Conclusion: Taken together, the data indicate that miR-458b-5p regulates ovarian GCs proliferation through Wnt/β-catenin signaling pathway by targeting <i>CTNNB1</i>, suggesting that miR-458b-5p and its target gene <i>CTNNB1</i> may potentially play a role in chicken ovarian follicular development.

Regional Homogeneity Brain Alterations in Schizophrenia: An Activation Likelihood Estimation Meta-Analysis

Xiaolei Qiu,Wenwen Xu,Rongrong Zhang,Wei Yan,Wenying Ma,Shiping Xie,Min Zhou 대한신경정신의학회 2021 PSYCHIATRY INVESTIGATION Vol.18 No.8

Objective Resting state functional magnetic resonance imaging (rsfMRI) provides a lot of evidence for local abnormal brain activity in schizophrenia, but the results are not consistent. Our aim is to find out the consistent abnormal brain regions of the patients with schizophrenia by using regional homogeneity (ReHo), and indirectly understand the degree of brain damage of the patients with drug-naive first episode schizophrenia (Dn-FES) and chronic schizophrenia. Methods We performed the experiment by activation likelihood estimation (ALE) software to analysis the differences between people with schizophrenia group (all schizophrenia group and chronic schizophrenia group) and healthy controls. Results Thirteen functional imaging studies were included in quantitative meta-analysis. All schizophrenia group showed decreased ReHo in bilateral precentral gyrus (PreCG) and left middle occipital gyrus (MOG), and increased ReHo in bilateral superior frontal gyrus (SFG) and right insula. Chronic schizophrenia group showed decreased ReHo in bilateral MOG, right fusiform gyrus, left PreCG, left cerebellum, right precuneus, left medial frontal gyrus and left anterior cingulate cortex (ACC). No significant increased brain areas were found in patients with chronic schizophrenia. Conclusion Our findings suggest that patients with chronic schizophrenia have more extensive brain damage than FES, which may contribute to our understanding of the progressive pathophysiology of schizophrenia.

TAp73 and ΔNp73 Have Opposing Roles in 5-aza-2'-Deoxycytidine-Induced Apoptosis in Breast Cancer Cells

Jing Lai,Fang Yang,Wenwen Zhang,Yanru Wang,Jing Xu,Wei Song,Guichun Huang,Jun Gu,Xiaoxiang Guan 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.8

The p73 gene contains an extrinsic P1 promoter and an intrinsic P2 promoter, controlling the transcription of the pro-apoptotic TAp73 isoform and the anti-apoptotic ΔΝp73 isoform, respectively. The DNA methylation status of both promoters act equally in the epigenetic transcriptional regulation of their relevant isoforms. The aim of this study was to analyze the different effects of these p73 isoforms in 5-aza-2'-deoxycytidine (5-aza-dC)-induced apoptosis in breast cancer cells. We investigated the effects of the DNA demethylation agent, 5-aza-dC, on the T-47D breast cancer cell line, and evaluated the methylation status of the p73 promoters and expression of TAp73 and ΔNp73. Furthermore, we assessed the expression of p53 and p73 isoforms in 5-aza-dC-treated T-47D cells and p53 knockout cells. 5-aza-dC induced significant anti-tumor effects in T-47D cells, including inhibition of cell viability, G1 phase arrest and apoptosis. This was associated with p73 promoter demethylation and a concomitant increase in TAp73 mRNA and protein expression. In contrast, the methylation status of promoter P2 was not associated with ΔNp73 mRNA or protein levels. Furthermore, demethylation of P2 failed to inhibit the expression of ΔNp73 with 5-aza-dC in the p53 knockdown cell model. Our study suggests that demethylation of the P1 and P2 promoters has opposite effects on the expression of p73 isoforms, namely up-regulation of TAp73 and down-regulation of ΔΝp73. We also demonstrate that p53 likely contributes to 5-aza-dC-induced ΔNp73 transcriptional inactivation in breast cancer cells.

Polar poly(n‑butyl acrylate)‑g‑polyacrylonitrile elastomer with high temperature elasticity and healability as flexible electronic substrate

Yuzhu Zheng,Deli Xu,Shiyou Tian,Manli Li,Wenwen Wang,Ke Liu 한국의류학회 2021 Fashion and Textiles Vol.8 No.1

In this work, graft copolymer poly (n-butyl acrylate)-g-polyacrylonitrile with poly (n-butyl acrylate) as backbones and polyacrylonitrile as side chains (PnBA-g-PAN) was synthesized by macromonomer method and emulsion polymerization. The macromonomer was synthesized by atom transfer radical polymerization and end-group modification. The chemical structures and thermal properties of macromonomer and graft copolymer were investigated by FTIR, GPC, NMR and TGA, etc. The mechanical properties of graft copolymer elastomer was also measured by uniaxial tensile test. Rheological properties at different temperature and mechanical property demonstrated that graft copolymer elastomer possessed elasticity until 180 oC because of cyclization of cyano groups. Ag nanowires@PnBA-g-PAN composite elastomer was developed, and the resulted material exhibited autonomic healing property on account of segments’ flexibility and dynamic interaction between Ag nanowires (AgNWs) and cyano groups. This is a general method for generation of elastomer with high temperature elasticity and fast self-healing. The composite elastomer has potential application in flexible electronic conductor.

The Review of New NDT Methods of Metal Material Fatigue Monitoring

Jingwei Dong,Qijian Wu,Wenwen Jiang,Qi Xu 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.8

Non-destructive testing (NDT) techniques have been employed in all kinds of fields successfully for many years. Newly developed and applied NDT methods of detecting and monitoring fatigue crack in metal material are reviewed in this paper. Some methods based on machine vision technique and image recognition techniques are introduced for at or near surface visible crack testing. As a newborn offspring of acoustic emission technique, electromagnetically induced acoustic emission methods also introduced for both visible and invisible fatigue cracks on metal structure. Nonlinear ultrasonic method and some other NDT methods presently available are mentioned, too. The principles, applied testing fields, data processing algorithms, advantages and disadvantages of these methods are discussed.

Stimuli-Responsive Polymers: Fundamental Considerations and Applications

Yongfeng Gao,Menglian Wei,Xue Li,Wenwen Xu,Andrews Ahiabu,Juliana Perdiz,Zining Liu,Michael J. Serpe 한국고분자학회 2017 Macromolecular Research Vol.25 No.6

Stimuli-responsive polymers are capable of changing their chemical and/ or physical properties in response to environmental stimuli. This unique feature has allowed stimuli-responsive polymers to be used in a variety of applications. In this review, we present a basic introduction to the theories that have been developed to describe polymer chains, brushes, and networks. We then detail numerous examples of how stimuli-responsive polymers can be used for sensing and biosensing, drug delivery, and as artificial muscles. While we focus the review on these particular areas, there are numerous other demonstrations of the applications of these fascinating materials, and we are certain that many applications have yet to be discovered.

Tissues Expression, Polymorphisms Identification of FcRn Gene and Its Relationship with Serum Classical Swine Fever Virus Antibody Level in Pigs

Liu, Yang,Wang, Chonglong,Liu, Zhengzhu,Xu, Jingen,Fu, Weixuan,Wang, Wenwen,Ding, Xiangdong,Liu, Jianfeng,Zhang, Qin Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.8

Neonatal Fc receptor (FcRn) gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G (IgG) and is responsible for IgG transport and stabilization. In this report, the 8,900 bp porcine FcRn genomic DNA structure was identified and putative FcRn protein included 356 amino acids. Alignment and phylogenetic analysis of the porcine FcRn amino acid sequences with their homologies of other species showed high identity. Tissues expression of FcRn mRNA was detected by real time quantitative polymerase chain reaction (Q-PCR), the results revealed FcRn expressed widely in ten analyzed tissues. One single nucleotide polymorphism (SNP) (HQ026019:g.8526 C>T) in exon6 region of porcine FcRn gene was demonstrated by DNA sequencing analysis. A further analysis of SNP genotypes associated with serum Classical Swine Fever Virus antibody (anti-CSFV) concentration was performed in three pig populations including Large White, Landrace and Songliao Black pig (a Chinese indigenous breed). Our results of statistical analysis showed that the SNP had a highly significant association with the level of anti-CSFV antibody (At d 20; At d 35) in serum (p = 0.008; p = 0.0001). Investigation of expression and polymorphisms of the porcine FcRn gene will help us in further understanding the molecular basis of the antibody regulation pathway in the porcine immune response. All these results indicate that FcRn gene might be regarded as a molecular marker for genetic selection of anti-CSFV antibody level in pig disease resistance breeding programmes.