Anti-inflammatory and Anti-oxidant Effects of PHBV/Collagen (PHCP) on Lipopolysaccharide-Induced Skin Inflammation

Jin-Yi Han3*, Xu Zi Guang, Jyung-Sik Kwak, Ki-Wan Oh, Han-Ik Bae 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.1

The anti-inflammatory effect of PHBV/Collagen (PHCP) was examined in a mouse model of lipopolysaccharide (LPS)-induced skin inflammation. Vascular permeability on the back skin was measured by the local accumulation of Evan’s blue dye after subcutaneous injection of LPS (30 μg site-1). Dye leakage in the skin showed a significant increase at 2 h after injection of LPS. This LPS-induced dye leakage was also completely inhibited by HO-1 inhibitor, ZnPP, and antioxidants, including methyl gallate, trolox, and mannitol. To study the possible mechanisms underlying the in vivo anti-inflammatory effect of PHCP against LPS-induced inflammation, we also examined the effects of PHCP on malondialdehyde (MDA) and glutathione levels in skin tissues and found that pretreatment with PHCP resulted in inhibited MDA elevation and a remarkable reduction of glutathione level. In addition, similar results were obtained after pretreatment with antioxidants, including trolox and mannitol, and HO-1 inhibitor, ZnPP. Histopathologically, an influx of neutrophils into the skin dermis was detected between 24 h and 72 h after LPS injection (30, 100 μg site-1), compared to control animals after injection of saline. This increase was greater in mice treated with 100 μg of LPS than in those treated with 30 μg of LPS and was significantly suppressed by pretreatment with PHCP, antioxidants, and HO-1 inhibitor. These results collectively suggest that PHCP has an anti-inflammatory effect against LPS-induced inflammation model in vivo and may be a good candidate for the skin tissue engineering biomedical application primarily through manipulation of the redox state.

RNAi suppression of nuclear receptor genes results in increased susceptibility to sulfoxaflor in brown planthopper, Nilaparvata lugens

Lu Xu,Chun-Qing Zhao,De-Jin Xu,Guang-Chun Xu,Xiao-Long Xu,Zhao-Jun Han,Ya-Nan Zhang,Zhong-Yan Gu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2

Nuclear receptors (NRs), which belong to a superfamily of ligand-dependent transcription factors, play essential roles in gene regulation to affect numerous physiological pathways. Twenty NR genes were identified in Nilaparvata lugens by using genomic, transcriptomic and GenBank databases, and categorized into NR0-NR6 subfamilies according to standard nomenclature. Among them, three NR geneswere classed into NR0, four into NR1, eight into NR2, one into NR3, one into NR4, two into NR5, and one into NR6, respectively. A phylogenetic tree of NRs from N. lugens and other representative species was constructed, which provided evolutionary insight into genetic distance. In order to investigate the NRs,whichwere induced by sulfoxaflor, time- and tissue-specific expression profiles of NR genes in fourth-instar nymphs were determined following LD50 sulfoxaflor treatment (0.28 ng/insect) and compared with control samples. NlUSP, NlE78, NlTLL, NlHR51, NlHR83, NlPNR and NlFTZ-F1 were highly expressed following 12–48 h of sulfoxaflor treatment. NlHR39 and NlDSF transcripts were detected in the head and the thorax, respectively. High-level and constitutive expression of NlHR3, NlTLL, NlHR83, NlFTZF1 and NlHR4 were found in the abdomen. Feeding of dsRNAs reduced the expression of NlHR3, NlUSP, NlTLL, NlHR83, NlPNR, NlFTZ-F1 and NlHR4 (35.48–49.77%) and caused significant nymph mortality (69.21–81.45%). These NRs, considered as insecticide targets, may play important function in sulfoxaflor detoxification.

The Gene Polymorphism of VMAT2 Is Associated with Risk of Schizophrenia in Male Han Chinese

Hongying Han,Xiaowei Xia,Huirong Zheng,Chongbang Zhao,Yanming Xu,Jiong Tao,Xianglan Wang 대한신경정신의학회 2020 PSYCHIATRY INVESTIGATION Vol.17 No.11

Objective To investigate the association between gene polymorphism of vesicular monoamine transporter type 2(VMAT2) and schizophrenia in Han Chinese population.Methods 430 patients with schizophrenia and 470 age-sex matched controls were recruited from four mental health centers. All patients were diagnosed by two psychiatrists based on the Structured Clinical Interview for DSM Disorders (SCID). The ligase detection reactions (LDR) method was used to assess the polymorphism of the two SNPs (rs363371 and rs363324) of VMAT2.Results No associations of two SNPs with schizophrenia was found. When we stratified males and females for the analysis, we found that that in the recessive model of rs363371, there was an obvious significant association between rs363371 and schizophrenia in males (OR=0.564, 95% CI=0.357–0.892, p=0.014) but not females. For the association between rs363324 and schizophrenia, no association was found in either males or females. No association was found when stratifying early-onset schizophrenia and late-onset schizophrenia.Conclusion Our findings indicate that both rs363371 and rs363324 were not associated with schizophrenia, while it seemed that the AA genotype of rs363371 plays a protective effect in male Chinese in developing schizophrenia.

On the Application of Game Theory to the Competition among Major Ports in NE Asia for T/S Cargos

Xu, Han(한욱),Gyei-Kark Park(박계각),Tae-Young Ahn(안태영) 한국지능시스템학회 2012 한국지능시스템학회논문지 Vol.22 No.2

동북아시아 경제의 급성장으로 인한 지역 물동량 및 해운시장 환경 변화에 따라서 부산항과 상해항은 동북아 물류 중심 허브가 되기 위하여 항만개발 및 항만 경쟁력을 강화해 왔으며, 최근에는 환적 컨테이너 화물 유치경쟁이 더욱 심화되고 있다. 따라서 본 연구에서는 동북아 주요 컨테이너 항만중 경쟁관계에 있는 부산항과 상해항을 대상으로 이 두 항만이 피더항의 환적화물을 유치하기 위한 경쟁의 게임모형을 수립하여 경쟁상황을 분석하였다. Due to rapid growth of East Asian economy, aome changes are being made in trade volume and shipping market of this area. Busan port in Korea and Shanghai port in China are continually increasing their investment in port development and competitiveness in order to become the logistics hub of Northeast Asia. Especially the competition for transshipment containers becomes more and more fierce. So in this study, we set a goal of increasing the port competitiveness on transshipment cargo by an analysis of competition strategies. We choose the Busan port and the Shanghai port as the research objects, games competition model and real data is used to analyze the two ports" price strategies and merket share. According to the results, some sdvices will be put forward to enhance competitiveness of the two ports.

Dynamic analysis of wind-vehicle-bridge system considering additional moments of non-uniform winds by wind shielding effect of multi-limb tower

Xu Han,Huoyue Xiang,Xuli Chen,Yong-Le Li 한국풍공학회 2023 Wind and Structures, An International Journal (WAS Vol.36 No.1

To evaluate the wind shielding effect of bridge towers with multiple limbs on high-speed trains, a wind tunnel test was conducted to investigate the aerodynamic characteristics of vehicles traversing multi-limb towers, which represented a combination of the steady aerodynamic coefficient of the vehicle-bridge system and wind environment around the tower. Subsequently, the analysis model of wind-vehicle-bridge (WVB) system considering the additional moments caused by lift and drag forces under nonuniform wind was proposed, and the reliability and accuracy of the proposed model of WVB system were verified using another model. Finally, the factors influencing the wind shielding effect of multi-limb towers were analyzed. The results indicate that the wind speed distributions along the span exhibit two sudden changes, and the wind speed generally decreases with increasing wind direction angle. The pitching and yawing accelerations of vehicles under nonuniform wind loads significantly increase due to the additional pitching and yawing moments. The sudden change values of the lateral and yawing accelerations caused by the wind shielding effect of multi-limb tower are 0.43 m/s2 and 0.11 rad/s2 within 0.4 s, respectively. The results indicate that the wind shielding effect of a multi-limb tower is the controlling factor in WVB systems.

Comparative study of homogeneous nucleation rate models for wet steam condensing flows

Xu Han,Zhonghe Han,Hengfan Li,Peng Li 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.12

To accurately describe the homogeneous nucleation process in a wet-steam dual-phase flow and to improve the reliability of computations of the condensing steam flow, classical homogeneous nucleation theories were derived and summarized based on the molecular agglomerate thermodynamic free energies. To obtain more accurate homogeneous nucleation rates, various modified homogeneous nucleation rate models were described. Experimental data were used in a comparative study of these models to select a nucleation rate model to provide calculated values for engineering projects. This study indicates that the main difference between different nucleation theories lies in the difference in the influence of temperature on the nucleation rate.

Experimental Study and Numerical Analysis on Mechanical Behavior of T-shape Stiffened Orthotropic Steel-concrete Composite Bridge Decks

Xu Jiang,Qing-Tian Su,Xu Han,Changyu Shao,Liang Chen 한국강구조학회 2017 International Journal of Steel Structures Vol.17 No.3

A new-type of orthotropic steel-concrete composite bridge deck system was developed, by casting the concrete overlay on the top of the orthotropic steel deck ribbed with T-shape steel members. To study its mechanical behavior (in terms of failure mode, load-deflection relationship, concrete crack initiation and propagation, strength, stiffness and so on), two new-type orthotropic steel-concrete composite bridge decks with different section dimensions were experimentally investigated and two reference decks (reinforced concrete deck and orthotropic steel deck) were also involved in the research for comparison. For the two new-type orthotropic steel-concrete composite decks, the average value of ultimate loads per width is 885.7kN, which is 2.35 and 1.61 times of that of the concrete and steel reference decks with almost the same section height. Experimental results proved that the composite deck can effectively control the crack initiation and propagation in the concrete and postpone the yielding of the steel bars and steel plates, due to the composite action between the concrete overlay and the underlying steel plate. Furthermore, the Finite Element (FE) model of the orthotropic steel-concrete composite deck was developed and validated by test results. A parametric study is conducted regarding to the stiffness of shear studs. With the validated FE model, stress distribution in the underlying steel plate and T-shape stiffeners and development of concrete cracking in the concrete overlay were characterized at different load levels.

Numerical Simulation of Seismic Waves with Peak Arrival Time and Amplitude-Frequency Correlation

Xu Han,Zhitao Wang,Ling-yun Peng,Jingyu Su,Lihua Wang 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.10

To improve the accuracy with which the non-stationary intensity characteristics of artificial acceleration-time-history curves are determined, a method of adjusting the frequency correlation between the artificial peak arrival time and amplitude spectrum is proposed. A calculation method for determining the peak arrival time to reflect the physical meaning of the phase-differenc espectrum was first deduced. Subsequently, the amplitude-distribution characteristics corresponding to the peak arrival times for different durations of natural earthquakes were analyzed. In accordance with the distribution characteristics of the reference amplitude, the frequency distribution of the artificial peak arrival time was divided into four parts: the beginning, strong-earthquake, attenuation, and tail sections. By adjusting the arrival frequency of peak points for the different earthquake durations considered, the corresponding relationship between the artificial peak arrival time and amplitude spectrum was determined to be more consistent with the general law of natural earthquakes. The sample calculation described in this paper demonstrates that adjusting the frequency correspondence between the artificial peak arrival time and amplitude spectrum greatly improves the accuracy of the non-stationary intensity characteristics of artificial acceleration-time-history curves.

Amperometric Determination of Nitrite at Poly(Methylene Blue)-Modified Glassy Carbon Electrode

Xu, Guang-Ri,Xu, Guifang,Xu, Ming-Lu,Zhang, Zhengqing,Tian, Yuan,Choi, Han-Nim,Lee, Won-Yong Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.2

Electrochemical characteristics of nitrite ion were investigated at a poly(methylene blue)-modified glassy carbon electrode by cyclic voltammetry and differential pulse voltammetry. The poly(methylene blue)-modified glassy carbon electrode exhibited enhanced anodic signals for nitrite. The effects of key parameters on the detection of nitrite were evaluated at the modified electrode, such as pH, accumulation time, and scan rate. Under optimum condition, the chemically modified electrode can detect nitrite in the concentration range $2.0{\times}10^{-6}$ to $5.0{\times}10^{-4}$ M with the detection limit of $2.0{\times}10^{-6}$ M and a correlation coefficient of 0.999. The detection of nitrite using the chemically modified electrode was not affected by common ions such as $Na^+$, $K^+$, $Ca^{2+}$, $Cl^-$, $HPO_4^{2-}$ and $H_2PO_4^- $. The modified electrode showed good stability and reproducibility. The practical application of the present method was successfully applied to the determination of nitrite ion in cabbage samples.

On-demand Allocation of Multiple Mutual-compensating Resources in Wireless Downlinks: a Multi-server Case

( Han Han ),( Yuhua Xu ),( Qinfei Huang ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.3

In this paper, we investigate the multi-resource allocation problem, a unique feature of which is that the multiple resources can compensate each other while achieving the desired system performance. In particular, power and time allocations are jointly optimized with the target of energy efficiency under the resource-limited constraints. Different from previous studies on the power-time tradeoff, we consider a multi-server case where the concurrent serving users are quantitatively restricted. Therefore user selection is investigated accompanying the resource allocation, making the power-time tradeoff occur not only between the users in the same server but also in different servers. The complex multivariate optimization problem can be modeled as a variant of 2-Dimension Bin Packing Problem (V2D-BPP), which is a joint non-linear and integer programming problem. Though we use state decomposition model to transform it into a convex optimization problem, the variables are still coupled. Therefore, we propose an Iterative Dual Optimization (IDO) algorithm to obtain its optimal solution. Simulations show that the joint multi-resource allocation algorithm outperforms two existing non-joint algorithms from the perspective of energy efficiency.