Angelica Sinensis Polysaccharide Induces Erythroid Differentiation of Human Chronic Myelogenous Leukemia K562 Cells

Wang, Lu,Jiang, Rong,Song, Shu-Dan,Hua, Zi-Sen,Wang, Jian-Wei,Wang, Ya-Ping Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Leukemia is a clonal disorder with blocked normal differentiation and cell death of hematopoietic progenitor cells. Traditional modalities with most used radiation and chemotherapy are nonspecific and toxic which cause adverse effects on normal cells. Differentiation inducing therapy forcing malignant cells to undergo terminal differentiation has been proven to be a promising strategy. However, there is still scarce of potent differentiation inducing agents. We show here that Angelica sinensis polysaccharide (ASP), a major active component in Dong quai (Chinese Angelica sinensis), has potential differentiation inducing activity in human chronic erythro-megakaryoblastic leukemia K562 cells. MTT assays and flow cytometric analysis demonstrated that ASP inhibited K562 cell proliferation and arrested the cell cycle at the G0/G1 phase. ASP also triggered K562 cells to undergo erythroid differentiaton as revealed by morphological changes, intensive benzidine staining and hemoglobin colorimetric reaction, as well as increased expression of glycophorin A (GPA) protein. ASP induced redistribution of STAT5 protein from the cytoplasm to the nucleus. Western blotting analysis further identified that ASP markedly sensitized K562 cells to exogenous erythropoietin (EPO) by activating EPO-induced JAK2/STAT5 tyrosine phosphorylation, thus augmenting the EPO-mediated JAK2/STAT5 signaling pathway. On the basis of these findings, we propose that ASP might be developed as a potential candidate for chronic myelogenous leukemia inducing differentiation treatment.

Robust H∞ Control for Switched Nonlinear Port-controlled Hamiltonian Systems

Zi-Ming Wang,Airong Wei,Xianfu Zhang 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.8

In the paper, the robust H∞ control for switched nonlinear port-controlled Hamiltonian (SNPCH) systems with external disturbances is investigated via the multiple Lyapunov functions method. Under two cases that the SNPCH system is subject to actuator saturation (AS) and the SNPCH system is without AS, a design approach for the switching robust H∞ feedback controller is developed to attenuate the external disturbances. The sufficient conditions are established for H∞ control of the corresponding closed-loop systems based on Hamilton-Jacobi inequality techniques. Furthermore, via the Hamiltonian realization method, the obtained results are applied to solve the H∞ control for switched nonlinear affine systems with external disturbances. Finally, two numerical examples are presented to illustrate the effectiveness of the proposed H∞ control methods.

Plant cover associated with aboveground net primary productivity (ANPP) mediates insect community composition in steppes of Northwest China

Zi-Hua Zhao,Gadi V.P. Reddy,Shuhua Wei,Mengmeng Zhu,Kaiyang Zhang,Hongqian Yu,Zhanjun Wang,Qi Jiang,Rong Zhang 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

Temperate steppe is one of the most important natural habitats for the conservation of arthropod and birdbiodiversity across the Eurasian Tectonic Plate. Since 1950, fragmentation of the steppe habitat has caused a lossof biodiversity and degradation of the species communities found in natural steppe. Therefore, in this study, bothplants and insects were sampled at 56 sites in the steppe biome of northwestern China to explore the effects ofplant community on insect community composition and diversity. The insect community structure varied in thefour different steppe types (meadow steppe, typical steppe, desert steppe, and steppe desert). Plant cover (diversity)was an important driving force, which could enhance number of families and abundance of an insectcommunity. Aboveground net primary productivity and water content of plants had no significant effects oninsect community, although the plant community as a whole did mediate insect composition and communitystructure. Future research should explore the ecological role of particular functional groups in plant and insectcommunities. Supplemental sowing to improve plant diversity in steppe habitat may be another strategy toenhance biodiversity and achieve sustainable management.

Lactation performance and rumen fermentation in dairy cows fed a diet with alfalfa hay replaced by corn stover and supplemented with molasses

Zi-Hai Wei,Shu-Lin Liang,Di-Ming Wang,Hong-Yun Liu,Metha Wanapat,Jian-Xin Liu 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.8

Objective: The objective of current study was to investigate the lactation performance and rumen fermentation characteristics of dairy cows fed a diet with alfalfa hay replaced by corn stover but supplemented with molasses. Methods: Sixteen Holstein cows in mid-lactation were randomly assigned to 1 of 2 dietary treatments: i) alfalfa based diet (AH), and ii) corn stover based diet supplemented with molasses (CSM). The experiment was conducted according to a 2×2 crossover design with 22-d each period, consisting of 17 d for adaptation and 5 d for data and samples collection. Results: Dry matter intake and milk yield were higher for cows fed AH than CSM (p<0.01). Milk protein content and nitrogen conversion were higher (p<0.05), while milk urea nitrogen was lower (p<0.01) for cows fed AH than CSM-fed cows. Contents of milk total solids, fat and lactose were not different between two groups (p>0.10). Total rumen volatile fatty acid concentration tended to be higher (p = 0.06) for cows fed AH than CSM-fed cows. Molar proportion of acetate was lower (p = 0.04), but valerate was higher (p = 0.02) in cows fed AH than CSM-fed cows. Rumen concentration of propionate, and isobutyrate, and ratio of acetate to propionate tended to be different (p<0.10) between two groups. The feed cost per kilogram of milk was lower in CSM than AH (p<0.01). No differences were found in feed efficiency and most plasma parameters tested (p>0.10). Conclusion: In comparison with AH diet, CSM diet could be fed to dairy cows without negative effect on feed efficiency, ruminal fermentation, but economically beneficial, indicating that CSM could be an alternative choice for dairy farms instead of AH to feed mid-lactation dairy cows.

Machining characteristics of USV-MF complex assisted WEDM-LS based on multi-physical coupling

Yan Wang,Shun-wen Yao,Zi-jun Ding,Chen-zhen Wu,Wei Xiong 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.8 No.2

Ultrasonic vibration (USV) and magnetic field (MF) complex assisted techniques are combined with low speed wire electrical discharge machining (USV-MF complex assisted WEDM-LS) to improve the machining performance of conventional WEDM-LS. Firstly, the simulation results of multi-physical coupling simulation model show that under the influence of ultrasonic vibration, the working fluid fluidity is greatly enhanced and its temperature is significantly decreased. Then, a three-phase flow model of bubble, working fluid and debris in the machining area is established to investigate the effects of ultrasonic vibration on bubble and debris, and the process of bubble rupture is simulated. The simulation results show that with the increase of ultrasonic frequency, the movement of bubble and debris becomes fiercer and fiercer, when ultrasonic frequency is 30 kHz and ultrasonic amplitude is 10 μm, the bubble is collapsed. Finally, comparison of the experimental results of conventional WEDM-LS, USV assisted WEDM-LS, MF assisted WEDM-LS and USV-MF complex assisted WEDM-LS in machining TiNi-01 shape memory alloy reveals that the ratio of normal discharge and the material removal rate are both increased, the workpiece surface roughness value is decreased and the workpiece surface quality is improved in the process of USV-MF complex assisted WEDM-LS.

Synthesis and Properties of Thioether-containing Aliphatic Polyesters

Xiao-Wei Kan,An Lyu,Qi-Yuan Wang,Ling-Gao Li,Zi-Chen Li 한국고분자학회 2018 한국고분자학회 학술대회 연구논문 초록집 Vol.43 No.2

Chemical constituents from Inula wissmanniana and their anti-inflammatory activities

Chunhui Wang,Xufeng Zhang,Panlei Wei,Xiangrong Cheng,Jie Ren,Shi Kai Yan,Wei Dong Zhang,Hui Zi Jin 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.12

Two new inositol derivatives, 4-acetate-1,2,3,5-tetrakis(2-methyl-2-butenoate) inositol (1) and 3-acetate-1,2,4,5-tetrakis(2-methyl-2-butenoate) inositol (2), onenew flavonolignan 23-o-acetylsilychristin A (3), and onenew thymol derivative 3-hydroxy-4-(2-hydroxy-1-methylethyl)benzoic acid (4), along with 13 known compounds,including ten flavonolignans (5–14) and three thymolderivatives (15–17) were isolated from the aerial part ofInula wissmanniana. The structures of new compoundswere elucidated by detailed spectroscopic analysis,including HRESIMS and 2D-NMR spectroscopic data. Allthe compounds were isolated from this plant for the firsttime. The anti-inflammatory activities of the compoundswere tested against LPS-induced NO production in RAW264.7 macrophages.

Silencing of long noncoding RNA PVT1 inhibits podocyte damage and apoptosis in diabetic nephropathy by upregulating FOXA1

Dong-Wei Liu,Jia-Hui Zhang,Feng-Xun Liu,Xu-Tong Wang,Shao-Kang Pan,Deng-Ke Jiang,Zi-Hao Zhao,Zhang-Suo Liu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

The number of patients with diabetic nephropathy (DN) is still on the rise worldwide, and this requires the development of new therapeutic strategies. Recent reports have highlighted genetic factors in the treatment of DN. Herein, we aimed to study the roles of long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) and histone 3 lysine 27 trimethylation (H3K27me3) in DN. A model of DN was established by inducing diabetes in mice with streptozotocin. Mouse podocyte clone 5 (MPC5) podocytes and primary podocytes were cultured in normal and high glucose media to observe cell morphology and to quantify PVT1 expression. The roles of PVT1 and enhancer of zeste homolog 2 (EZH2) were validated via loss-of-function and gain-of-function in vitro experiments to identify the interactions among PVT1, EZH2, and forkhead box A1 (FOXA1). The podocyte damage and apoptosis due to PVT1 and FOXA1 were verified with in vivo experiments. PVT1 was highly expressed in MPC5 and primary podocytes in DN patients and in cultures grown in high glucose medium. A large number of CpG (C-phosphate-G) island sites were predicted at the FOXA1 promoter region, where PVT1 recruited EZH2 to promote the recruitment of H3K27me3. The silencing of PVT1 or the overexpression of FOXA1 relieved the damage and inhibited the apoptosis of podocytes in DN, as was evidenced by the upregulated expression of synaptopodin and podocin, higher expression of Bcl-2, and lower expression of Bax and cleaved caspase-3. The key findings of this study collectively indicate that the suppression of lncRNA PVT1 exerts inhibitory effects on podocyte damage and apoptosis via FOXA1 in DN, which is of clinical significance.

Pelvic Solitary Plasmacytoma: Computed Tomography and Magnetic Resonance Imaging Findings with Histopathologic Correlation

Ying Wang,Xiu-Liang Zhu,Mohamad Wasil Peeroo,Zi-Hua Qian,Dan Shi,Shu-Mei Wei,Ri-Sheng Yu 대한영상의학회 2015 Korean Journal of Radiology Vol.16 No.1

To describe the imaging features of pelvic solitary plasmacytoma and to correlate them with the pathologic grade. A retrospective study was performed on the imaging features of 10 patients with a histological diagnosis of pelvic solitary plasmacytoma. The imaging studies were assessed for bone expansion, cortical destruction, signal intensity/density of soft tissue mass and enhancement manifestations, which were then correlated to the pathologic grade. The imaging features of pelvic solitary plasmacytoma revealed 3 different types: multilocular type (n = 5), unilocular type (n = 2) and complete osteolytic destruction type (n = 3) on computed tomography and MRI. Pathologically, the tumors were classified into low, intermediate and high grades. Features such as multilocular change, perilesional osteosclerosis, slight expansion, local bone cortex disruptions and masses inside bone destruction, often suggest a low-grade solitary plasmacytoma; complete osteolytic destruction, huge soft tissue mass, and osseous defects imply a higher pathologic grade. Pelvic solitary plasmacytoma has various imaging manifestations, while a slight expansile osteolytic feature with multilocular change or homogeneous enhancement highly suggests its diagnosis. The distinctive imaging features of pelvic solitary plasmacytoma are well correlated to the pathologic grade.

Beneficial effects of natural Jeju groundwaters on lipid metabolism in high-fat diet-induced hyperlipidemic rats

Yan-chao Wang,Jin-miao Lu,Hui-zi Jin,Ai-niu Ma,Jin-yang Zhang,Nian Gong,Qi Xiao,Bin Zhu,Ying-fang Lv,Na Yu,Wei-dong Zhang,Yong-xiang Wang 한국영양학회 2014 Nutrition Research and Practice Vol.8 No.2

BACKGROUND: Groundwater is believed to possess many beneficial effects due to its natural source of various minerals. In this study, we examined the effects of natural Jeju groundwater S1 (SamdasooTM), S2 and S3 pumped up from different locations of Jeju Island, Korea, along with local tap water, on body weight gain, serum lipids and lipoproteins, and liver histopathology in high-fat diet-induced hyperlipidemic rats. MATERIALS/METHODS: Rats were randomly and equally divided into 6 groups. Different water samples were supplied to the hyperlipidemic rats as their daily drinking water and the widely-used anti-hyperlipidemic drug simvastatin was used as a positive control. Body weight, serum lipids and lipoproteins were measured weekly. Liver weight, liver index and liver histopathology were examined after the execution of the rats. RESULTS: After drinking Jeju groundwaters for two months, S2 but not S3 significantly reduced weight growth and serum triglycerides levels and increased high density lipoprotein-C (HDL-C) without affecting total cholesterol or LDL-C. S1 and particularly S2 significantly reduced the severity of liver hypertrophy and steatosis. All Groundwaters had much higher contents of vanadium (S3>S2>S1>>tap water) whereas S1 and S2 but not S3 markedly blocked autoxidation of ferrous ions. CONCLUSION: Jeju Groundwater S1 and particularly S2 exhibit protective effects against hyperlipidemia and fatty liver and hypothesize that the beneficial effect of Jeju Groundwaters may be contributed from blockade of autoxidation of ferrous ions rather than their high contents of vanadium.