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      • KCI등재

        Mite and Booklouse Fauna From Vacuumed Dust Samples From Beijing

        Jin-Lu Sun,Lian Shen,Jun Chen,Jin-Miao Yu,Jia Yin 대한천식알레르기학회 2014 Allergy, Asthma & Immunology Research Vol.6 No.3

        A significant-source of allergens come from house dust that contain particles derived from arthropods, molds, and pet dander. This study evaluatedmite and booklouse fauna from vacuumed dust samples in Beijing China (a temperate zone). Our survey was carried out in Beijing in the homes ofmite allergic patients who visited our Allergy Department. In total, 38 homes were selected for the collection of dust samples by vacuuming, fromDecember 2008 to January 2010. The flotation method was used to isolate mites from house dust. Permanent slides were prepared for mite specimensand mites were identified and counted under a microscope. In total, 1,798 separate mite and insect specimens were found in 345 dust samplestaken from 38 homes. A total of 95 individual Dermatophagoides (D) siboney were detected in 35 dust samples from 19 homes (representing5.3% of all mite and insect species found in house dust); in addition, this mite was found to co-exist with D. farinae (Hughes, 1961) in 33 dust samples. Our results demonstrated the presence D. siboney that co-existed with D. farinae in house dust in Beijing China (a temperate zone).

      • KCI등재

        Genome-wide analyses and expression patterns under abiotic stress of LAC gene family in pear (Pyrus bretschneideri)

        Lu Chunyi,Yang Tianyuan,Zhang Yiwu,Miao Xingchen,Jin Cong,Xu Xiaoyong 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.3

        Plant laccases are copper-containing glycoproteins oxidizing monolignol, which is a key regulator for lignin polymerization and deposition in plant cell walls. In plants, the LAC gene family has been identifed and functionally characterized in Arabi�dopsis, rice, and other plant species. Whereas, no systematic analysis of LAC genes was explored in pear (Pyrus bretschnei�deri), especially their biological roles in stress responses. In this study, a total of 40 P. bretschneideri LACs (PbLACs) were identifed from the pear genome, among which 32 PbLACs members were distributed on 14 chromosomes, and 8 PbLACs were mapped onto scafold contigs. Subsequently, the chromosome localizations, gene structures, conserved domains, gene duplication were analyzed comprehensively and bioinformatically. Phylogenetic analysis demonstrated that the PbLACs were classifed into seven groups. Synteny analysis exhibited that whole-genome duplication (WGD)/segmental duplication was essential for the expansion of PbLAC family. In detail, the 26 PbLACs duplicated gene pairs were taken place at the two WGD events. Further, our results showed that purifying selection presented as the primary force to drive the evolution of PbLACs. Notably, the transcriptomic data were used for exploring the biological roles of PbLACs in response to abiotic stresses (cold, drought, and salt) in pear. We found that the expression of certain PbLACs was signifcantly induced by drought, cold or salt treatments, suggesting that these PbLACs are potentially key genes for further functional characterization in pear in the future. Taken together, our results not only contribute to an enhanced understanding of the evolutional complexity of PbLAC gene family but also provide a good platform for functional studies of PbLAC genes in response to various abiotic stresses.

      • KCI등재

        Beneficial effects of natural Jeju groundwaters on lipid metabolism in high-fat diet-induced hyperlipidemic rats

        Yan-chao Wang,Jin-miao Lu,Hui-zi Jin,Ai-niu Ma,Jin-yang Zhang,Nian Gong,Qi Xiao,Bin Zhu,Ying-fang Lv,Na Yu,Wei-dong Zhang,Yong-xiang Wang 한국영양학회 2014 Nutrition Research and Practice Vol.8 No.2

        BACKGROUND: Groundwater is believed to possess many beneficial effects due to its natural source of various minerals. In this study, we examined the effects of natural Jeju groundwater S1 (SamdasooTM), S2 and S3 pumped up from different locations of Jeju Island, Korea, along with local tap water, on body weight gain, serum lipids and lipoproteins, and liver histopathology in high-fat diet-induced hyperlipidemic rats. MATERIALS/METHODS: Rats were randomly and equally divided into 6 groups. Different water samples were supplied to the hyperlipidemic rats as their daily drinking water and the widely-used anti-hyperlipidemic drug simvastatin was used as a positive control. Body weight, serum lipids and lipoproteins were measured weekly. Liver weight, liver index and liver histopathology were examined after the execution of the rats. RESULTS: After drinking Jeju groundwaters for two months, S2 but not S3 significantly reduced weight growth and serum triglycerides levels and increased high density lipoprotein-C (HDL-C) without affecting total cholesterol or LDL-C. S1 and particularly S2 significantly reduced the severity of liver hypertrophy and steatosis. All Groundwaters had much higher contents of vanadium (S3>S2>S1>>tap water) whereas S1 and S2 but not S3 markedly blocked autoxidation of ferrous ions. CONCLUSION: Jeju Groundwater S1 and particularly S2 exhibit protective effects against hyperlipidemia and fatty liver and hypothesize that the beneficial effect of Jeju Groundwaters may be contributed from blockade of autoxidation of ferrous ions rather than their high contents of vanadium.

      • SCIESCOPUSKCI등재

        Effects of enzymolysis and fermentation of Chinese herbal medicines on serum component, egg production, and hormone receptor expression in laying hens

        Mei Hong Jiang,Tao Zhang,Qing Ming Wang,Jin Shan Ge,Lu Lu Sun,Meng Qi Li,Qi Yuan Miao,Yuan Zhao Zhu Asian Australasian Association of Animal Productio 2024 Animal Bioscience Vol.37 No.1

        Objective: In the present study, we aimed to investigate the effects of enzymolysis fermentation of Chinese herbal medicines (CHMs) on egg production performance, egg quality, lipid metabolism, serum reproductive hormone levels, and the mRNA expression of the ovarian hormone receptor of laying hens in the late-laying stage. Methods: A total of 360 Hy-Line Brown laying hens (age, 390 days) were randomly categorized into four groups. Hens in the control (C) group were fed a basic diet devoid of CHMs, the crushed CHM (CT), fermented CHM (FC), and enzymatically fermented CHM (EFT) groups received diets containing 2% crushed CHM, 2% fermented CHM, and 2% enzymatically fermented CHM, respectively. Results: Compared with crushed CHM, the acid detergent fiber, total flavonoids, and total saponins contents of fermented CHM showed improvement (p<0.05); furthermore, the neutral and acid detergent fiber, total flavonoids, and total saponins contents of enzymatically fermented CHM improved (p<0.05). At 5 to 8 weeks, hens in the FC and EFT groups showed increased laying rates, haugh unit, albumin height, yolk color, shell thickness, and shell strength compared with those in the C group (p<0.05). Compared with the FC group, the laying rate, albumin height, and Shell thickness in the EFT group was increased (p<0.05). Compared with the C, CT, and FC groups, the EFT group showed reduced serum total cholesterol and increased serum luteinizing hormone levels and mRNA expressions of follicle stimulating hormone receptor and luteinizing hormone receptor (p<0.05). Conclusion: These results indicated that the ETF group improved the laying rate and egg quality and regulated the lipid metabolism in aged hens. The mechanism underlying this effect was likely related to cell wall degradation of CHM and increased serum levels of luteinizing hormone and mRNA expression of the ovarian hormone receptor.

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