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- 저자 : Wang Shan (검색결과 412 건)
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Wang, Shan,Wang, Ting,Wang, Tao,Jia, Lintao Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.11
Inducible and reversible gene silencing in desired types of cells is instrumental for deciphering gene functions using cultured cells or in vivo models. However, efficient conditional gene knockdown systems remain to be established. Here, we report the generation of an inducible expression system for short hairpin RNA (shRNA) targeted to PTEN, a well-documented dual-specificity phosphatase involved in tumor suppression and ontogenesis. Upon induction by doxycycline (DOX), the reverse tetracycline transcriptional activator (rtTA) switched on the concomitant expression of GFP and a miR-30 precursor, the subsequent processing of which released the embedded PTEN-targeted shRNA. The efficacy and reversibility of PTEN knockdown by this construct was validated in normal and neoplastic cells, in which PTEN deficiency resulted in accelerated cell proliferation, suppressed apoptosis, and increased invasiveness. Transgenic mice harboring the conditional shRNA-expression cassette were obtained; GFP expression and concurrent PTEN silencing were observed upon ectopic expression of rtTA and induction with Dox. Therefore, this study provides novel tools for the precise dissection of PTEN functions and the generation of PTEN loss of function models in specific subsets of cells during carcinogenesis and ontogenesis.
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Shan Wang,Lintao Jia,Ting Wang,Tao Wang 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.11
Inducible and reversible gene silencing in desired types of cells is instrumental for deciphering gene functions using cultured cells or in vivo models. However, efficient conditional gene knockdown systems remain to be established. Here, we report the generation of an inducible expression system for short hairpin RNA (shRNA) targeted to PTEN, a well-documented dual-specificity phosphatase involved in tumor suppression and ontogenesis. Upon induction by doxycycline (DOX), the reverse tetracycline transcriptional activator (rtTA) switched on the concomitant expression of GFP and a miR-30 precursor, the subsequent processing of which released the embedded PTEN-targeted shRNA. The efficacy and reversibility of PTEN knockdown by this construct was validated in normal and neoplastic cells, in which PTEN deficiency resulted in accelerated cell proliferation, suppressed apoptosis, and increased invasiveness. Transgenic mice harboring the conditional shRNAexpression cassette were obtained; GFP expression and concurrent PTEN silencing were observed upon ectopic expression of rtTA and induction with Dox. Therefore, this study provides novel tools for the precise dissection of PTEN functions and the generation of PTEN loss of function models in specific subsets of cells during carcinogenesis and ontogenesis.
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Dynamic Analysis of a 5D Fractional-order Hyperchaotic System
Shan Wang,Shan Wang 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.3
In this paper, the fractional-order 5D hyperchaotic system is proposed based on the hyperchaotic Lorenzsystem. Fractional-order chaotic systems are often three- or four-dimensional. There are few results about highdimension fractional-order systems. For this 5D hyperchaotic system, the stability of equilibrium points is analyzedby means of the stability theory of fractional systems. Then the fractional bifurcation is investigated. It is foundthat the system admits bifurcations with varying fractional-order and parameters, respectively. Under differentbifurcation parameters, some conditions ensuring the bifurcations are presented. Finally, numerical simulations arepresented to confirm the given analytical results.
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Shan-Shan Wang,Hai-Xiang Sun 연세대학교의과대학 2014 Yonsei medical journal Vol.55 No.3
Purpose: Blastocyst transfer has been recommended to raise the implantation rate without affecting the pregnancy rate. The objective of this meta-analysis is to systematicallyevaluate whether the live birth rate and other pregnancy outcomes can be improved by blastocyst transfer compared with cleavage-stage embryos transfer. Materials and Methods: EMBASE and MEDLINE databases were searched for papers published between March 2004 and March 2013. An extensive range of the electronic databases yielded initially 317 studies from which seven trials met the inclusion criteria for further analysis. Our outcome measures were the live birth rate, clinical pregnancy rate, implantation rate, ongoing pregnancy rate, multiplepregnancy rate, first trimester miscarriage rate and ectopic pregnancy rate. Fixed effects models were chosen to calculate the odds ratio (OR). Results: Seven trials (n=1446 cases) were finally analyzed. Compared with cleavage-stage embryostransfer, the blastocyst transfer was statistically significantly associated with an increase in clinical pregnancy rate [OR 1.43; 95% confidence interval (CI), 1.15-1.78], implantation rate (OR 1.38; 95% CI, 1.09-1.74) and ongoing pregnancy rate (OR 2.15; 95% CI, 1.57-2.94), and also a reduction in the probability of first trimestermiscarriage rate (OR 0.51; 95% CI, 0.30-0.87). The improvement in the live birth rate was also observed (OR 1.77; 95% CI, 1.32-2.37). Moreover, there was no evidence of difference in multiple pregnancy and ectopic pregnancy rates. Conclusion: The available evidences suggest that live birth and other pregnancy outcomes after fresh in vitro fertilization or intracytoplasmic sperm injection (IVF/ICSI) are significantly improved following blastocyst transfer as compared to cleavage-stage embryo transfer.
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王珊珊(Wang Shan-shan) 고려대학교 한자한문연구소 2007 동아한학연구 Vol.- No.3
The thesis is the continuance of another paper 'The Historical Phonetic Change in Phonetic-compound Series'. By using the materials in both Chinese language and Tibeto-Burman languages, take the Phonetic-compound Series of 'Si[?]' as the example, so as to explain the historical phonetic change of these Phonetic-compound series and make an attempt on the reconstruction of these Phonetic-compound series.
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Li-Jun Zuo,Shu-Yang Yu,Fang Wang,Yanghui Xia,Ying-Shan Piao,Yang Du,Teng-Hong Lian,Rui-Dan Wang,Qiu-Jin Yu,Ya-Jie Wang,Xiao-Min Wang,Piu Chan,Sheng-Di Chen,Yongjun Wang,Wei Zhang 대한신경과학회 2016 Journal of Clinical Neurology Vol.12 No.2
Background and Purpose The aim of this study was to identify the clinical characteristics and potential mechanisms relevant to pathological proteins in Parkinson’s disease (PD) patients who experience fatigue. Methods PD patients (n=102) were evaluated using a fatigue severity scale and scales for motor and nonmotor symptoms. The levels of three pathological proteins—α-synuclein oligomer, β-amyloid (Aβ)1-42, and tau—were measured in 102 cerebrospinal fluid (CSF) samples from these PD patients. Linear regression analyses were performed between fatigue score and the CSF levels of the above-listed pathological proteins in PD patients. Results The frequency of fatigue in the PD patients was 62.75%. The fatigue group had worse motor symptoms and anxiety, depression, and autonomic dysfunction. The CSF level of α-synuclein oligomer was higher and that of Aβ1-42 was lower in the fatigue group than in the non-fatigue group. In multiple linear regression analyses, fatigue severity was significantly and positively correlated with the α-synuclein oligomer level in the CSF of PD patients, after adjusting for confounders. Conclusions PD patients experience a high frequency of fatigue. PD patients with fatigue have worse motor and part nonmotor symptoms. Fatigue in PD patients is associated with an increased α-synuclein oligomer level in the CSF
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Flowering and flowering genes: from model plants to orchids
Shan-Li Wang,Hye Ryun An,Chii-Gong Tong,Seonghoe Jang 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.2
Floral transition in model plants including Arabidopsis and rice has been studied extensively through molecular geneticapproaches. Many genetic factors in diff erent fl owering pathways, which depend mainly on photoperiod, vernalization,autonomous and ambient temperature are regulated coordinately to control fl oral induction. However, for the ornamentalplants orchids, the molecular mechanisms underlying the fl oral transition are still unclear. Recently, genes with potentialfl owering-related functions have been identifi ed in diff erent orchid species and their functional roles have also been characterized/examined using homologous or heterologous systems. In this review, we summarize the molecular networks offl owering genes and their regulation as revealed in model plants such as Arabidopsis and rice, and also describe the recentdiscoveries/studies on fl owering genes in several commercially representative orchid species providing a perspective onorchid fl owering research. In addition, our recent results through transgenic approaches with ectopic expression of Hd3a , arice fl origen gene for the induction of precocious fl owering in Phalaenopsis orchids are also discussed.
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Analysis Of the Total Electrical Length Extremum Of TSSIR Using Lagrange Multipliers Method
Wang Shan,Sun Yu,Wang Yao,Yu Yiqiang 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.1
A systematic approach to determine the extreme value of total electrical length for tri-section stepped impedance resonator (TSSIR) is proposed in this paper. It utilizes the Lagrange multipliers method to search the extremum condition. Result demonstrates that the maximum resonator length of TSSIR is limited to triple that of the corresponding uniform impedance resonator (UIR). Spurious resonant frequency of λ/2 TSSIR is mainly analysized under the extremum condition (θ1≠θ2≠θ3). Through comparison between the proposed approach and previously assumed condition (θ1=θ2=θ3), the staircase discontinuity of transmission line can be reduced using the paper theory to achieve same harmonic ratio. Last, a simulated example is presented to verify correctness of the method. This paper aims to provide a theoretical basis and general guideline for the filter design using TSSIR.
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