N-Acetyl-L-Cysteine에 의한 생쥐 골수유래 가지세포의 기능적 활성화 저해

정영주(Young-joo Jeong),맹형건(Hyung Gun Maeng),김민규(Min Kyu Kim),강재승(Jae Seung Kang),이왕재(Wang Jae Lee),황영일(Young-il Hwang) 대한해부학회 2008 Anatomy & Cell Biology Vol.41 No.2

N-acetyl-L-cysteine (NAC)은 thiol기를 포함하는 화합물로서, glutathione (GSH)의 전구체로 작용하여 포유류 세포 내에서 항산화제로 작용한다. 또한 항염기능이 있으며 호산구나 B세포, 가지세포 (dendritic cell, DC)와 같은 면역세포 들에 여러 가지 영향을 미치는 것으로 알려져 있다. 특히 가지세포에 작용하여 활성화를 억제하거나 가지세포에 의한 Th2 반응 유도에 관여한다고 알려져 있다. 그러나 이들 연구는 세부적인 사항에 있어서 그 결과가 서로 상치하는바가 많으며, 또한 조절T세포의 관점에서는 연구된 바가 없다. 따라서 본 연구에서는 NAC 처리가 가지세포 활성화에 미치는 영향을 재확인하였고, NAC 처리된 가지세포의 T세포 활성 능력 저하, 또는 Th2 반응 유도 여부를 알아보았다. 활성화 시 가지세포에서 증가하는 활성산소기 (reactive oxygen species)는 NAC 처리로 낮아져서, NAC이 가지세포에 항산화작용을 나타냄을 확인하였다. NAC 처리로 가지세포에서 보조자극인자인 CD40과 CD86의 발현이 저해되었으며, 활성화 시 정상적으로 낮아지는 포식기능은 처리된 NAC의 농도에 비례하게 보존되었다. 활성화 시 분비되는 IL-6, IL-10, IL-12는 모두 감소하였다. 이러한 NAC-DC와 함께 배양한 T세포의 증식이나 Th1 cytokine인 IFN-γ, Th2 cytokine인 IL-5의 분비가 모두 저하되어 Th1/Th2의 편중 없이 가지세포의 T세포 자극능력이 전반적으로 감소하였음을 나타내었다. 또한 T세포 배양액에서 IL-10과 TGF-β의 농도 역시 NAC-DC로 자극된 경우에 현저히 줄어서, NAC-DC에 의한 T세포 증식 감소 등은 조절T세포 유도에 의한 것이 아니라 T세포 무반응이 유도된 때문임을 나타내 주었다. N-acetyl-L-cysteine (NAC) is a thiol-containing compound and acts as a precursor for glutathione (GSH). It behaves as an antioxidant in mammalian cells and also exerts anti-inflammatory effects. NAC is also known to affect several immune cells including eosinophils, B cells, T cells, and dendritic cells (DC) in many aspects. Even though it has been reported that NAC inhibits DC activation and shifts the immune response to Th2, these studies exhibit some contradictory results in detail and do not give any information with respect to the induction of regulatory T cells. In this study, we re-analyzed the effects of NAC on DC during their activation. We also evaluated whether it induced T cell anergy, Th1/Th2 shift, or regulatory T cells. NAC suppressed the elevation of intracellular reactive oxygen species during DC activation. In parallel, it down-regulated surface expression of CD40 and CD86, suppressed the decrease of phagocytic function, lowered the secretion of cytokines such as IL-6, IL-10, and IL-12. All these effects showed dose-dependency. Thus, it seems likely that NAC inhibited DC activation with regard to their phenotype and cytokine secretion. When we evaluated the T cell-stimulating capacity of these NAC-DC, T cell proliferation and secretion of both Th1 (IFN-γ) and Th2 cytokine (IL-5) were decreased. This implies that the T cell-stimulating activity of NAC-DC decreased without any shift to Th1 or Th2 cytokine (IL-5). The secretion of IL-10 and TGF-β in the supernatants were also decreased, which suggests that the decrease of T cell proliferation and cytokine secretion is due to the induction of T cell anergy, rather than regulatory T cells.

2,3-Dehydrosilybin Suppresses IL-31-Associated Pruritus Factors in Astrocytes and Microglia

Ji Hyeon Park,Jae Young Shin,Feng Wang,Suping Hao,Da Jeong Shin,Seon Il Jang,Byoung Ok Cho 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Chronic pruritus is the main symptom that increases the suffering of patients in hypersensitivity disorder disease. IL-31 is a pruritic cytokine with a primary objective to control itch. A 2,3-dehydrosilybin (DHS) is a type of flavonoid extracted from the seeds of milk thistle. DHS has been reported to have hepatoprotective, angiogenic, and antioxidant effects. This study investigated the effect of DHS pretreatment on IL-31-associated pruritus in astrocytes and microglia. Pretreatment with DHS inhibited the production of IL-31 by lipopolysaccharide (LPS) and interferon gamma (IFN-γ) stimulation in microglia. Pretreatment with DHS inhibited the phosphorylation of MAPK, STAT1 and NF-κB by LPS plus IFN-γ stimulation in microglia. In addition, DHS suppressed the expression of IL-31 receptor A in IL-31-treated astrocytes. DHS also inhibited lipocalin2 production in IL-31 stimulated astrocytes. Taken together, DHS has potential as a therapeutic agent for symptom relief by down-regulating the IL-31-mediated pruritus mechanism in microglia and astrocytes.

Antipruritic effect of 2,3-dehydrosilybin in IL-6-induced astrocyte and chloroquine-treated mice by targeting STAT3 signaling pathways

Byoung Ok Cho,Jae Young Shin,Ji Hyeon Park,Feng Wang,Suping Hao,Da Jeong Shin,Seon Il Jang 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Chronic pruritus is a symptom that reduces the quality of life of patients with inflammatory skin disease. Persistent activation of astrocytic signal transducer and activator of transcription 3 (STAT3) contributes to the elevation of chronic pruritus. STAT3 activation increases lipocalin-2 (LCN2) expression and enhances pruritus. A 2,3-dehydrosilybin (DHS) is a type of flavonoid extracted from the seeds of milk thistle. DHS has been reported to have hepatoprotective, angiogenic, and antioxidant effects. In this study, the inhibitory effect of DHS on chronic pruritus was investigated in IL-6-treated astrocytes and chloroquine-injected mice. As a result, DHS prevented STAT3 activation and LCN2 production in IL-6-treated astrocytes. Moreover, DHS inhibited scratching and inhibited the expression of glial fibrillary acidic protein (GFAP) in chloroquine-injected mice. It also reduced the level of inflammatory cytokines in the mice serum. In conclusion, it was demonstrated that DHS suppressed itch through the STAT3 signaling pathway. Thus our results suggest that DHS can prevent and/or treat chronic itch.

Polylysine을 입힌 배양면이 GH₃ 뇌하수체 종양 세포에 미치는 영향에 관한 연구

김정진,백상호,황덕호,황영일,이왕재,이병란 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.3

숫돌 형상 변화에 따른 연삭가공면 특성

왕덕현,김원일,강신엽,우정윤 경남대학교 신소재연구소 1997 論文集 Vol.9 No.-

숫돌 형상 변화에 따른 연삭가공시 나타나는 온도의 변화를 연삭가공면의 특성으로 파악하는 실험적 연구를 하였다. 숫돌의 형상은 슬롯형태로 폭과 각도의 변화에 의하여 변경시켰으며, 또한 일반숫돌인 경우 특성을 비교 분석하였다. 슬롯형 숫돌인 경우 슬롯의 폭이 10mm이고, 각도가 30°때 낮은 연삭온도를 나타내고 있었다. 표면거칠기 상태는 일반숫돌인 경우보다 슬롯형 숫돌일 경우 대체로 표면상태가 나쁘다는 것을 알았으며, 슬롯의 폭이 6mm이고 각도가 45°인 숫돌과 일반숫돌의 특성을 비교해 볼 때 유사한 경향을 나타내고 있었다. The characteristics of ground surface by different shapes of wheel were found by grinding temperature and surface roughness. The shapes of wheel are changed by slots, which has different width and helical angle, and compared with the case of general one. Lower grinding temperature was obtained for 30°helical angle and width of 10mm. The characteristics of surface roughness of slotted wheels were found to be rough, but the value of roughness for the slotted wheel with 45°helical angel and width of 6mm was similar tendency for the general wheel.

수면 박탈이 주간 졸음 및 피로감과 집중력에 미치는 영향

이규일,김정란,왕성근,신석철 대한생물치료정신의학회 2001 생물치료정신의학 Vol.7 No.2

Study objectives : The purpose of this study was to evaluate the effect of sleep deprivation on daytime sleepiness, fatigue, and attention measured by Vienna Test System and VAS. Participants : 9 healthy, normal young male college students participated in this study. Participating subjects were in good physical and psychological health and were asymtomatic as to sleep/wake complaints. Intervention : Subjects visited our laboratory by 08 : 00 at the first day, and they had baseline Vienna Test(at 9 : 00, 11 : 00, 14 : 00, and 16 : 00). Each time, the subjects completed VAS for sleepiness and fatigue before they underwent and Vienna Test to evaluate their attention and vigilance. They had 4 hours of sleep at 1st day, 2 hours at 2nd days, and total sleep deprivation at 3rd day and repeated the same process as those of the first day. Result : As compared to baseline, subjects had significantly increased daytime sleepiness and fatigue and decreased vigilance, but no significant difference in attention. The more sleep deprivation duration increased, the more daytime sleepiness and fatigue increased. Conclusion : Additional study is needed to get objective dat for daytime sleepiness and fatigue and to correlate among daytime sleepiness, fatigue, attention and vigilance.

미세형상가공시 센서융합을 이용한 공구 마멸 및 파손 메커니즘 검출

임정숙,왕덕현,김원일,이윤경 한국공작기계학회 2002 한국공작기계학회 춘계학술대회논문집 Vol.2002 No.-

A successful on-line monitoring system for conventional machining operations has the potential to reduce cost, guarantee consistency of product quality, improve productivity and provide a safer environment for the operator. In fine-shape machining, typical signs of tool problems such as vibration, noise, chip flow characteristics and visual signs are almost unnoticeable without the use of special equipment. These characteristics increase the importance of automatic monitoring in fine-shape machining; however, sensing and interpretation of signals are more complex. In addition, the shafts of the micro-tools break before the typical extensive cutting edge of the tool gets damaged. In this study, the existence of a relationship between the characteristics of the cutting force and tool usage was investigated, and tool breakage detection algorithm was developed and the following results are obtained. In data analysis, didn't use a relative error compare which mainly used in established experiment and investigated tool breakage detection algorithm in time domain which can detect AE and cutting force signals more effective and accurate.

Amplification of transglutaminase 2 enhances tumor-promoting inflammation in gastric cancers

Cho Sung-Yup,오유미,Jeong Eui Man,박상희,Dakeun Lee,Xiaorui Wang,Qiqi Zeng,Hongyu Qin,Fang Hu,Hui Gong,Xi Liu,Guanjun Zhang,나득채,이지은,채지수,서윤석,공성호,Hyuk-Joon Lee,Jong-Il Kim,박한수,Chengsheng Zhang,양한광,Charles Lee 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Tumor-promoting inflammation is a hallmark of cancer and is highly associated with tumor progression, angiogenesis, and metastasis. Tumor-associated macrophages (TAMs) are major drivers of tumor-promoting inflammation, but due to the complexity of the tumor microenvironment, the detailed regulatory mechanisms are still under investigation. Here, we investigated a novel role for transglutaminase 2 (TGM2) in the development of tumor-promoting inflammation and recruitment of TAMs to gastric cancer (GC) tissues. When estimated by array comparative genomic hybridization and droplet digital PCR, the copy numbers of the TGM2 gene were amplified in 13.6% (14/103) of GC patients and positively associated with TGM2 expression. Gene set enrichment analysis of expression microarray data for GC samples with high or low TGM2 expression showed that increased TGM2 expression was associated with tumor-promoting inflammation in GC. In addition, the expression of TGM2 was correlated with the expression of markers for macrophages, neutrophils, blood vessels, and lymphatic vessels. Overexpression of TGM2 in GC cells augmented the IL-1β-induced secretion of macrophage-recruiting chemokines and NF-κB activation. TGM2 protein levels were associated with the expression levels of the macrophage marker CD163 in human GC tissue samples. Moreover, GC patients with high expression of TGM2 had a worse prognosis than those with low expression of TGM2. These results suggest TGM2 as a novel regulator of the tumor microenvironment of GC and provide a promising target for constraining tumor-promoting inflammation.

DLE and Myricitrin attenuate IL-6-induced astrocyte activation and pruritus by targeting STAT3 signaling pathways

Jae Young Shin,Byoung Ok Cho,Ji Hyeon Park,Da Jeong Shin,Feng Wang,Suping Hao,Eun Seo Kang,Seon Il Jang 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Diospyros lotus (date plum) is a deciduous plant native to Asia including Korea and China. In traditional medicine, it has been used as an anticancer, antidiabetic, and antipyretic agent. Recently, the effect of Diospyros lotus on the improvement of sensitive skin was also reported. Chronic pruritus is one of the most difficult to manage symptoms of inflammatory skin disease. Recently, it was found that activation of STAT3 in astrocytes contributes to chronic pruritus. In this study, the effects of Diospyros lotus leaf extract (DLE) and its main component myricitrin on pruritus were investigated in in vitro and in vivo. Astrocytes were pretreated with DLE and myricitrin and stimulated with IL-6 to measure activation of STAT3 and production of lipocalin-2 (LCN2). We also investigated the effects of DLE and myricitrin on itch in chloroquine-induced itch mouse model. DLE and myricitrin blocked STAT3 activation and inhibited the release of LCN2 in astrocytes. Moreover, DLE and myricitrin inhibited the scratching behavior and inhibited the expression of glial fibrillary acidic protein (GFAP) in chloroquine-injected mice. Collectively, these studies suggest that modulation of DLE and myricitrin signaling pathways contribute to pruritus inhibition, thus suggesting potential for the prevention and/or treatment of pruritus caused by hypersensitivity skin conditions.

전치 태반의 임상적 고찰

홍관표,이성동,김왕수,정종일,장하종,정혁,한세준 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

This study was undertaken for the clinical evaluation and statistical analysis on the 38 cases of placenta previa who were admitted and treated from March 1, 1988 to Feb. 28, 1991. The results were as follows : 1. The incidence of placenta previa was one per 53 deliveries or 1.90% 2. There were 57.9 % total placenta previa, 21.1 % partial placenta previa, 13.1 % low-lying placenta previa and 7.9% marginal placenta previa. 3. Placenta previa has occured more often in multipara (63.2%) than primigravida 4. More than one-third (38.8%) cases of placenta previa had delayed admission until labor occured for some time following vaginal spotting has first time been noticed. More instructive prenatal education should be emphasized 5. Lower segment transverse section rate was 63.2%, classical cesarean section rate was 15.8% and cesarean hysterectomy was 9.8%. The rest of them were treated by vaginal deliveries of 5 cases(13.2%) 6. The perinatal loss associated with placenta previa was considered still higher : the premature death was 15.8% and term death was 2.6%. The problem of prevention of prematurity still remains to be studied. 7. There was no maternal mortality in our study