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Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.
Kim, Tae Rang,Kim, Su Un,Shin, Young,Kim, Ji Young,Lee, Sang Me,Kim, Jung Hun The Korean Society of Food Science and Nutrition 2013 Preventive Nutrition and Food Science Vol.18 No.4
In this study, the quick HPLC-MS/MS method for the simultaneous separation of 2-acetyl-4(5)-tetrahydroxybuthylimidazole (THI) and 4-(5)-methylimidazole (4-MI) in alkaline medium was used for caramel color and processed foods in Korea. After a simple sample pretreatment, 51 4-MI-labeled samples were positive for 4-MI and 2 also contained THI. The concentration of 4-MI was 260.5~24, $499.3{\mu}g/kg$ in caramel color, less than $LOD{\sim}1,712.5{\mu}g/kg$ in sauce, 1,242.3, $5,972.2{\mu}g/kg$ in balsamic vinegar, $2,118.3{\sim}5,802.4{\mu}g/kg$ in complex seasoning, $82.7{\sim}5,110.6{\mu}g/kg$ in curry, and $29.9{\sim}464.4{\mu}g/kg$ in soft drinks. The recovery rate of 4-MI was 97.1~111.0% in sauce and 81.9~110.0% in powder and that of THI was 83.6{\sim}106.4% in sauce and 61.2{\sim}99.4% in powder. Our results concluded a safe amount of 4-MI and THI compared to the limit of Korea additive code but the processed foods do not have a limit of caramel color and 4-MI in Korea. Therefore, research and monitoring of 4-MI and THI is needed for processed foods in Korea.
Kim, In‐,Suk,Jeong, Young‐,Hoon,Park, Yongwhi,Yoon, Seong‐,Eun,Kwon, Tae Jung,Park, Jeong Rang,Hwang, Seok‐,Jae,Koh, Eun‐,Ha,Kwak, Choong Hwan,Hwang, Jin‐,Yong,Kim, Blackwell Publishing Ltd 2012 British journal of clinical pharmacology Vol.73 No.4
<P><B>WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT</B></P><P>• Compared with standard dual antiplatelet therapy, adjunctive cilostazol to dual antiplatelet therapy ('triple antiplatelet therapy’) has a potential to reduce ischemic event occurrence after percutaneous coronary intervention.</P><P>• The pharmacokinetic and pharmacodynamic effects of clopidogrel have been significantly influenced by the enzyme activity of the <I>ABCB1 C3435T</I> and the <I>CYP2C19</I> system.</P><P>• For the pharmacokinetics of cilostazol, genetic polymorphisms of the <I>CYP3A5</I> and <I>CYP2C19</I> have been associated with the substantial interindividual variability in healthy volunteers.</P><P><B>WHAT THIS STUDY ADDS</B></P><P>• Loss‐of‐function polymorphism of the <I>CYP2C19</I> gene, but not the <I>ABCB1 C3435T</I> and <I>CYP3A5*3</I> genes, affects the antiplatelet effect of triple antiplatelet therapy.</P><P>• Most of extensive and intermediate East Asian metabolizers (0 or 1 <I>CYP2C19</I> loss‐of‐function allele) show adequate platelet inhibition when treated with triple antiplatelet therapy after percutaneous coronary intervention.</P><P>• However, carriage of 2 <I>CYP2C19</I> loss‐of‐function alleles is still associated with the risk of high platelet reactivity (defined by by 5 µM ADP‐induced maximal platelet aggregation >46%), which clinical impact needs to be validated in future clinical trials.</P><P><B>AIMS</B> Although adjunctive cilostazol to dual antiplatelet therapy can reduce the risks of clinical events after percutaneous coronary intervention (PCI), whether genetic polymorphism can influence the pharmacodynamics of this regimen has not been evaluated.</P><P><B>METHODS</B> One hundred and twenty‐seven patients treated with PCI and taking triple antiplatelet therapy (≥1 month) were enrolled. Platelet reactivity was assessed by conventional aggregometry and the VerifyNow P2Y12 assay. High on‐treatment platelet reactivity (HPR) was defined as 5 µ<SMALL>m</SMALL> ADP‐induced maximal platelet reactivity (Agg<SUB>max</SUB>) >46%. <I>CYP3A5</I>*<I>3</I>, <I>CYP2C19</I>*<I>2/</I>*<I>3</I> and <I>ABCB1</I> 3435C > T were genotyped.</P><P><B>RESULTS</B> <I>CYP3A5</I>*3 and <I>ABCB1</I> 3435C > T variants did not affect the antiplatelet effect of triple antiplatelet therapy. For non‐carriers, one and two carriers of the <I>CYP2C19</I> loss‐of‐function (LOF) allele, Agg<SUB>max</SUB> consecutively increased after the addition of 5 µ<SMALL>m</SMALL>[mean (95% confidence intervals): 24.6% (20.8 to 28.5%) <I>vs.</I> 28.7% (25.4 to 32.0%) <I>vs.</I> 32.3% (25.8 to 38.7%), <I>P =</I> 0.062, respectively] and 20 µ<SMALL>m</SMALL> ADP [34.2% (29.3 to 39.0%) <I>vs.</I> 41.7% (37.8 to 45.6%) vs. 44.9% (37.9 to 51.9%), <I>P</I> = 0.007, respectively]. Likewise, late platelet reactivity and P2Y12 reaction units proportionally changed according to the number of <I>CYP2C19</I> LOF alleles. HPRs were observed in 9.2% of subjects: 6.3%, 7.4% and 20.0% with 0, 1 and 2 carriers of <I>CYP2C19</I> LOF allele(s) (<I>P</I> = 0.099). In multivariate analysis, carriage of two <I>CYP2C19</I> LOF alleles was a significant predictor for the prevalence of HPR (odds ratio 5.78, 95% CI 1.21, 27.78, <I>P</I> = 0.028).</P><P><B>CONCLUSION</B> Among PCI‐treated patients, the effect of triple antiplatelet therapy is influenced by the <I>CYP2C19</I> LOF allele. Its clinical benefit needs to be validated according to the <I>CYP2C19</I> metabolic phenotype in future clinical trials. [Adjunctive Cilostazol Versus High Maintenance dose ClopidogrEL in Acute Myocardial Infarction Patients According to CYP2C19 Polymorphism (ACCEL‐AMI‐2C19), NCT00915733 and Adjunctive Cilostazol Versus High Maintenance‐dose Clopidog
Evaluation of Pesticides Residues in Agricultural Products for Raw Juice in Seoul
Tae-rang Kim,Young-hee Choi,Mi-sun Kim,Young-hee Jin,Mi-ok Song,Eun-jung Han,Chun-yeoung Lee,Hee-jin Choi,Min-jung Kim,Hyun-Jeong Kim,Eun-sun Yun,Yong-Seung Shin 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11
In this study, we conducted the levels of pesticide residue as well as the risk assessment for pesticides which exceeded maximum residue limits(MRLs). A total of 345 Agricultural Products (239 Vegetables and 106 Fruits) for raw juice were distributed from the southern area of Seoul from January to August in 2021 for 280 types of pesticides were analyzed by multi-residue method in Korean Food Code. As a result, Occurrences of violation Max Residue Limits(MRLs) of pesticide residues were found in the 4 samples(3 kales and 1 celery, 1.2%). However, the levels of residual pesticides in Fruits were not exceeded MRLs. Pesticide residue levels in 60 cases(33 fruits and 27 vegetables, 17.4%) reveal to the below the standard limits. The detection rate of pesticides in fruits for juice was 31.1%, which was about three times higher than the 11.3% of vegetables. The most commonly exceeded their MRLs pesticides residues were three types of pesticides(paclobutrzol, fluopyram and vinclozolin). The safety levels of these residual pesticides that exceeded MRLs was assessed using the ratio of estimated daily intake(EDI) and acceptable daily intake(ADI). The results showed that their ratios were between 0.34~1.43% of ADI, which indicated that these pesticides were within the safe limits.
Tae Rang Kim,Su Un Kim,Young Shin,Ji Young Kim,Sang Me Lee,Jung Hun Kim 한국식품영양과학회 2013 Preventive Nutrition and Food Science Vol.18 No.4
In this study, the quick HPLC-MS/MS method for the simultaneous separation of 2-acetyl-4(5)-tetrahydroxybuthylimidazole (THI) and 4-(5)-methylimidazole (4-MI) in alkaline medium was used for caramel color and processed foods in Korea. After a simple sample pretreatment, 51 4-MI-labeled samples were positive for 4-MI and 2 also contained THI. The concentration of 4-MI was 260.5∼24,499.3 μg/kg in caramel color, less than LOD∼1,712.5 μg/kg in sauce, 1,242.3, 5,972.2 μg/kg in balsamic vinegar, 2,118.3∼5,802.4 μg/kg in complex seasoning, 82.7∼5,110.6 μg/kg in curry, and 29.9∼464.4 μg/kg in soft drinks. The recovery rate of 4-MI was 97.1∼111.0% in sauce and 81.9∼110.0% in powder and that of THI was 83.6∼106.4% in sauce and 61.2∼99.4% in powder. Our results concluded a safe amount of 4-MI and THI compared to the limit of Korea additive code but the processed foods do not have a limit of caramel color and 4-MI in Korea. Therefore, research and monitoring of 4-MI and THI is needed for processed foods in Korea.