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Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.
황혜정,김태랑,윤광로 中央大學校 食糧資源硏究所 1999 食糧資源硏究所 論文集 Vol.18 No.1
The mineral contents were analyzed for Kimchi by atomic absorption spectrometer and spectrophotometry. The Na contents of tested commercial Kimchi ranged 16118.95~28633.97ppm, Ca 582.87~736.85ppm, K 1305.88~ 2395.91ppm, P 360.11~398.73ppm, Mg 114.50~175.69ppm, and Fe 14.63~19.05ppm. The mineral contents of laboratory produced Kimchi were Na 16550.87ppm, Ca 655ppm, K 1015.34ppm, P 359.88ppm, Mg 115.17ppm, and Fe 16.22ppm, respectively. Comparing mineral contents of Kimchi, Na, Ca and K contents were higher than P, Mg and Fe contents. Among the mineral content of Kimchi, Na was the major element.
Evaluation of Pesticides Residues in Agricultural Products for Raw Juice in Seoul
Tae-rang Kim,Young-hee Choi,Mi-sun Kim,Young-hee Jin,Mi-ok Song,Eun-jung Han,Chun-yeoung Lee,Hee-jin Choi,Min-jung Kim,Hyun-Jeong Kim,Eun-sun Yun,Yong-Seung Shin 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11
In this study, we conducted the levels of pesticide residue as well as the risk assessment for pesticides which exceeded maximum residue limits(MRLs). A total of 345 Agricultural Products (239 Vegetables and 106 Fruits) for raw juice were distributed from the southern area of Seoul from January to August in 2021 for 280 types of pesticides were analyzed by multi-residue method in Korean Food Code. As a result, Occurrences of violation Max Residue Limits(MRLs) of pesticide residues were found in the 4 samples(3 kales and 1 celery, 1.2%). However, the levels of residual pesticides in Fruits were not exceeded MRLs. Pesticide residue levels in 60 cases(33 fruits and 27 vegetables, 17.4%) reveal to the below the standard limits. The detection rate of pesticides in fruits for juice was 31.1%, which was about three times higher than the 11.3% of vegetables. The most commonly exceeded their MRLs pesticides residues were three types of pesticides(paclobutrzol, fluopyram and vinclozolin). The safety levels of these residual pesticides that exceeded MRLs was assessed using the ratio of estimated daily intake(EDI) and acceptable daily intake(ADI). The results showed that their ratios were between 0.34~1.43% of ADI, which indicated that these pesticides were within the safe limits.
( Tae Rang Kim ),( Ki Hwan Park ),( Mi Ra Jang ),( Young Hee Choi ),( Eun Hee Kim ),( Chae Man Choi ),( Sung Kyu Park ),( In Sil Yu ),( In Sook Hwang ),( Ki Young Han ),( Moo Sang Kim ),( Jung Hun Kim 한국응용생명화학회(구 한국농화학회) 2013 Journal of Applied Biological Chemistry (J. Appl. Vol.56 No.1
This study was performed to analyze 48 kinds of pesticide residues using gas chromatography (GC)/nitrogen phosphorous detector, GC/micro electron capture detector, GC/mass selective detector, and high performance liquid chromatograph/diode array detector in 186 fresh ginseng samples collected in the Seoul area from 2010 to 2011. Fresh ginseng dietary intakes were estimated using the data from the 2009 Korea National Health and Nutrition examination survey. Residual pesticides were detected in 79 samples (42.5%) with eight different fungicides. Only 20 samples (10.8%) exceeded the maximum residue limits (MRLs) for pesticides registered by the Korea Food & Drug Administration. Among them, tolclofos-methyl residues (10.2%) exceeded the MRL for fresh ginseng in 18 ginseng seedlings and one of the two-year old fresh ginseng plants, and the residual level in just one ginseng seedling violated the MRL for pyrimethanil. The results showed that residual pesticides levels in marketable fresh ginseng around Seoul were relatively safe. The percent acceptable daily intake (%ADI) was calculated using pesticide residues in fresh ginseng and dietary intakes of fresh ginseng. The risk caused by pesticide residues in fresh ginseng was very low.
Kim, Tae Rang,Kim, Su Un,Shin, Young,Kim, Ji Young,Lee, Sang Me,Kim, Jung Hun The Korean Society of Food Science and Nutrition 2013 Preventive Nutrition and Food Science Vol.18 No.4
In this study, the quick HPLC-MS/MS method for the simultaneous separation of 2-acetyl-4(5)-tetrahydroxybuthylimidazole (THI) and 4-(5)-methylimidazole (4-MI) in alkaline medium was used for caramel color and processed foods in Korea. After a simple sample pretreatment, 51 4-MI-labeled samples were positive for 4-MI and 2 also contained THI. The concentration of 4-MI was 260.5~24, $499.3{\mu}g/kg$ in caramel color, less than $LOD{\sim}1,712.5{\mu}g/kg$ in sauce, 1,242.3, $5,972.2{\mu}g/kg$ in balsamic vinegar, $2,118.3{\sim}5,802.4{\mu}g/kg$ in complex seasoning, $82.7{\sim}5,110.6{\mu}g/kg$ in curry, and $29.9{\sim}464.4{\mu}g/kg$ in soft drinks. The recovery rate of 4-MI was 97.1~111.0% in sauce and 81.9~110.0% in powder and that of THI was 83.6{\sim}106.4% in sauce and 61.2{\sim}99.4% in powder. Our results concluded a safe amount of 4-MI and THI compared to the limit of Korea additive code but the processed foods do not have a limit of caramel color and 4-MI in Korea. Therefore, research and monitoring of 4-MI and THI is needed for processed foods in Korea.