Primary pyomyositis in North India: a clinical, microbiological, and outcome study

( Susheel Kumar ),( Ashish Bhalla ),( Rajveer Singh ),( Navneet Sharma ),( Aman Sharma ),( Vikas Gautam ),( Surjit Singh ),( Subhash Varma ) 대한내과학회 2018 The Korean Journal of Internal Medicine Vol.33 No.2

Background/Aims: Pyomyositis is an infective condition with primary involvement of the skeletal muscles. There is sparse recent literature on patients with pyomyositis. Methods: This study was carried out at emergency services of a tertiary care center located in subtropical area of Indian subcontinent. Results: Sixty-two patients of primary pyomyositis formed the study cohort. Mean age of occurrence was 29.9 ± 14.8 years. There were 54 men. Twelve patients had underlying medical diseases. Muscle pain was seen in all 62 patients. Forty-eight patients (77.4%) had the fever. Most common site of involvement was thigh muscles (n = 29, 46.8%). Forty-nine patients (79%) presented in the suppurative stage of illness. Patients with comorbidities were older (age: median 36 years [interquartile range (IQR), 25 to 47] vs. 24 years [IQR, 16 to 35], p = 0.024), had higher culture positivity with gram-negative organisms (8/9 [88.89%] vs. 6/29 [20.69%], p = 0.001). Importantly, higher number of these patients received inappropriate antibiotics initially. Patients with positive pus culture result had higher complication rate (32/38 [84.21%] vs. 10/18 [55.56%], p = 0.044). Six patients (9.7%) had in-hospital mortality. Lower first-day serum albumin, initial inappropriate antibiotic therapy, and advanced form of the disease at presentation were associated with increased in-hospital mortality. Conclusions: Primary pyomyositis is not an uncommon disease entity. Patients with comorbidities were more likely to receive initial inappropriate antibiotic therapy. Patients with positive pus culture report had the higher rate of complications. Lower first-day serum albumin, initial inappropriate antibiotic therapy and advanced form of the disease at presentation were associated with increased in-hospital mortality.

Integration of Particle Swarm Optimization (PSO) and Machine Learning to Improve Classification Accuracy During Antenna Design

Susheel Kumar Singh,Mukesh Kumar,Jeetendra Singh 한국전기전자재료학회 2023 Transactions on Electrical and Electronic Material Vol.24 No.3

Flexibility and generalization of antenna analysis and synthesis using artificial neural networks have attracted enormous attention in the field of microwave-strip antennas (MSAs). Various problems can be solved using Particle Swarm Optimization PSO by applying social connections. A Swarm of particles (agents) is applied to find the best possible solution. These agents seek the space coordinates associated with the best solution each particle has so far achieved. Predicting response times with a trained neural network is almost equivalent to measuring or simulating them. The proposed work has integrated an optimization mechanism into machine learning which improves the reliability of the model. The simulation shows the categorization process utilizing machine learning for antenna design. As a result of using the PSO optimizer, the accuracy, precision, f1-score, and recall value of the simulations have significantly improved. An improvement of 4%, 10%, and 5% is observed in accuracy, recall value, and in precision by using the optimization technique. The result proves that the integration of machine learning into the optimizer increases the suggested model's dependability.

Optimizing Nutrient Management Strategies for Rice-Wheat System in the Indo-Gangetic Plains and Adjacent Region for Higher Production Profitability, Nutrient Use Efficiency and Ensuring Food Security

Vinod Kumar Singh,Brahma S. Dwivedi,Kaushik Majumdar,Meenu Rani,Susheel K. Singh 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Genetic Variability Based on Tandem Repeat Numbers in a Genomic Locus of 'Candidatus Liberibacter asiaticus' Prevalent in North East India

Singh, Yanglem Herojit,Sharma, Susheel Kumar,Sinha, Bireswar,Baranwal, Virendra Kumar,Singh, N. Bidyananda,Chanu, Ngathem Taibangnganbi,Roy, Subhra S.,Ansari, Meraj A.,Ningombam, Arati,Devi, Ph. Sobit The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.6

The genetic variability of 'Candidatus Liberibacter asiaticus' (CLas) population associated with huanglongbing (HLB) disease of citrus in North Eastern (NE) region of India, a geographically locked region, and home for the diversity of many citrus species was analyzed on the basis of tandem repeat numbers (TRN) in variable CLIBASIA_01645 genomic loci. Fifty-five CLas strains sampled from different groves of NE Hill (NEH) region of India were in single amplicon group, but there was remarkable genetic variability in TRNs. The TRN in HLB-associated CLas strains varied from 0-21 and two novel repeat motifs were also identified. Among the NE population of CLas, TRN5 and TRN9 were most frequent (total frequency of 36.36%) followed by TRN4 (14.55%) and TRN6, TNR7 with a frequency of 12.73% each. Class II type CLas genotypes (5 < TRN ≤ 10) had highest prevalence (frequency of 60.00%) in the samples characterized in present study. Class I (TRN ≤ 5) genotypes were second highest prevalent (29.09%) in the NEH region. Further analysis of genetic diversity parameters using Nei's measure (H value) indicated wide genetic diversity in the CLas strains of NE India (H value of 0.58-0.86). Manipur CLas strains had highest genetic variability (0.86) as compared to Eastern, Southern and Central India. The R10 values (TRN ≤ 10/TRN > 10) of NE CLas population was 10.43 (73/7), higher from other regions of India. Present study conclusively reported the occurrence of high genetic variability in TRN of CLas population in North East Indian citrus groves which have evolved to adapt to the specific ecological niche.

Genetic Variability Based on Tandem Repeat Numbers in a Genomic Locus of ‘Candidatus Liberibacter asiaticus’ Prevalent in North East India

Yanglem Herojit Singh,Susheel Kumar Sharma,Bireswar Sinha,Virendra Kumar Baranwal,N. Bidyananda Singh,Ngathem Taibangnganbi Chanu,Subhra S. Roy,Meraj A. Ansari,Arati Ningombam,Ph. Sobita Devi,Ashis Ku 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.6

The genetic variability of ‘Candidatus Liberibacter asiaticus’ (CLas) population associated with huanglongbing (HLB) disease of citrus in North Eastern (NE) region of India, a geographically locked region, and home for the diversity of many citrus species was analyzed on the basis of tandem repeat numbers (TRN) in variable CLIBASIA_01645 genomic loci. Fifty-five CLas strains sampled from different groves of NE Hill (NEH) region of India were in single amplicon group, but there was remarkable genetic variability in TRNs. The TRN in HLB-associated CLas strains varied from 0-21 and two novel repeat motifs were also identified. Among the NE population of CLas, TRN5 and TRN9 were most frequent (total frequency of 36.36%) followed by TRN4 (14.55%) and TRN6, TNR7 with a frequency of 12.73% each. Class II type CLas genotypes (5 < TRN ≤ 10) had highest prevalence (frequency of 60.00%) in the samples characterized in present study. Class I (TRN ≤ 5) genotypes were second highest prevalent (29.09%) in the NEH region. Further analysis of genetic diversity parameters using Nei’s measure (H value) indicated wide genetic diversity in the CLas strains of NE India (H value of 0.58-0.86). Manipur CLas strains had highest genetic variability (0.86) as compared to Eastern, Southern and Central India. The R10 values (TRN ≤ 10/TRN > 10) of NE CLas population was 10.43 (73/7), higher from other regions of India. Present study conclusively reported the occurrence of high genetic variability in TRN of CLas population in North East Indian citrus groves which have evolved to adapt to the specific ecological niche.

Developmental stage-dependent differential gene expression of superoxide dismutase isoenzymes and their localization and physical interaction network in rice (Oryza sativa L.)

Krishna Nath,이춘환,Susheel Kumar,Roshan Sharma Poudyal,양영남,Rupak Timilsina,박유신,Jayamati Nath,Puneet Singh Chauhan,Bijaya Pant 한국유전학회 2014 Genes & Genomics Vol.36 No.1

Superoxide dismutase (SOD) isoenzymes areessential for scavenging excess reactive oxygen species inliving organisms. So far, expression pattern of SOD isoenzymesgenes along leaf development plus their sub-cellularlocalization and physical interaction network have notyet been clearly elucidated. Using multiple bioinformaticstools, we predicted the sub-cellular localizations of SODisoforms and described their physical interactions in rice. Using in silico approaches, we obtained several evidencesfor existence of seven SOD genes and a SOD copperchaperone gene. Their transcripts were differentiallyexpressed along with different developmental stage of riceleaf. Finally, we performed quantitative real time-polymerasechain reaction (qRT-PCR) to validate in silico differential expression pattern of SOD genes experimentally. Expression of two cytosolic cCuZn-SODs was highduring the whole vegetative stage. Two plastidic Fe-SODswere found and their expression levels were very low andstarted to increase from the late vegetative stage. Theirexpression patterns were very similar to each other, indicatingthe formation of heterodimer. However, theirexpression patterns are different from those for ArabidopsisFe-SODs. The expression of pCuZn-SOD was very high inthe early developmental stage, but qRT-PCR results weredifferent, which remains for further study. From the resultson the differential expression of SOD genes, we canunderstand the role of each SOD gene and even predicttheir role under certain circumstances based on in silicoanalysis.

Acupressure as an Adjunct to Pharmacological Treatment for Depression, Anxiety, and Stress in Patients with Knee Osteoarthritis

Meenu Rani,Lokendra Sharma,Uma Advani,Susheel Kumar 사단법인약침학회 2020 Journal of Acupuncture & Meridian Studies Vol.13 No.4

Background: Osteoarthritis is the most common type of arthritis. Depression, anxiety, and stress are associated with knee osteoarthritis. Objectives: The aim of the study was to check the effectiveness of acupressure therapy with pharmacological treatment on pain, depression, anxiety, and stress in patients with knee osteoarthritis and to assess the effect of pain improvement on psychological health. Methods: Eligible 212 patients with knee osteoarthritis were divided into two groups (intervention and control group). The intervention group (n = 106) received acupressure therapy in combination with pharmacological treatment, whereas the control group (n = 106) continued pharmacological treatment only. Pain and psychological symptoms were measured using the visual analog scale and Depression Anxiety Stress Scale-21. Pearson's correlation was used to check the effect of pain improvement on psychological health. Results: Patients of both groups reported severe pain initially. On analyzing the results after completion of the study, it was found that patients in the intervention group scored better on the pain scale (p < 0.001) and DASS-21 (p ≤ 0.0001). However, it may be noted that reduction in the DASS-21 score was not found to be significant for the control group (p = 0.08). Pearson's correlation coefficients value ranged from 0.231 to 0.412 for DASS-21 (p < 0.05). Conclusions: On analysis, it can be concluded that acupressure can be used as add-on therapy in combination with conventional treatment (pharmacological treatment), which may assist in pain reduction. The reduction in pain directly contributes to improvement in the physiological wellness among patients with knee osteoarthritis.