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RISS 인기검색어
- 검색키워드
- 저자 : Rupak Timilsina (검색결과 2 건)
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Krishna Nath,이춘환,Susheel Kumar,Roshan Sharma Poudyal,양영남,Rupak Timilsina,박유신,Jayamati Nath,Puneet Singh Chauhan,Bijaya Pant 한국유전학회 2014 Genes & Genomics Vol.36 No.1
Superoxide dismutase (SOD) isoenzymes areessential for scavenging excess reactive oxygen species inliving organisms. So far, expression pattern of SOD isoenzymesgenes along leaf development plus their sub-cellularlocalization and physical interaction network have notyet been clearly elucidated. Using multiple bioinformaticstools, we predicted the sub-cellular localizations of SODisoforms and described their physical interactions in rice. Using in silico approaches, we obtained several evidencesfor existence of seven SOD genes and a SOD copperchaperone gene. Their transcripts were differentiallyexpressed along with different developmental stage of riceleaf. Finally, we performed quantitative real time-polymerasechain reaction (qRT-PCR) to validate in silico differential expression pattern of SOD genes experimentally. Expression of two cytosolic cCuZn-SODs was highduring the whole vegetative stage. Two plastidic Fe-SODswere found and their expression levels were very low andstarted to increase from the late vegetative stage. Theirexpression patterns were very similar to each other, indicatingthe formation of heterodimer. However, theirexpression patterns are different from those for ArabidopsisFe-SODs. The expression of pCuZn-SOD was very high inthe early developmental stage, but qRT-PCR results weredifferent, which remains for further study. From the resultson the differential expression of SOD genes, we canunderstand the role of each SOD gene and even predicttheir role under certain circumstances based on in silicoanalysis.
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Kim, Hyo Jung,Hong, Sung Hyun,Kim, You Wang,Lee, Il Hwan,Jun, Ji Hyung,Phee, Bong-Kwan,Rupak, Timilsina,Jeong, Hana,Lee, Yeonmi,Hong, Byoung Seok,Nam, Hong Gil,Woo, Hye Ryun,Lim, Pyung Ok Oxford University Press 2014 Journal of experimental botany Vol.65 No.14
<P>Leaf senescence is a finely tuned and genetically programmed degeneration process, which is critical to maximize plant fitness by remobilizing nutrients from senescing leaves to newly developing organs. Leaf senescence is a complex process that is driven by extensive reprogramming of global gene expression in a highly coordinated manner. Understanding how gene regulatory networks involved in controlling leaf senescence are organized and operated is essential to decipher the mechanisms of leaf senescence. It was previously reported that the trifurcate feed-forward pathway involving <I>EIN2</I>, <I>ORE1</I>, and <I>miR164</I> in <I>Arabidopsis</I> regulates age-dependent leaf senescence and cell death. Here, new components of this pathway have been identified, which enhances knowledge of the gene regulatory networks governing leaf senescence. Comparative gene expression analysis revealed six senescence-associated NAC transcription factors (TFs) (ANAC019, AtNAP, ANAC047, ANAC055, ORS1, and ORE1) as candidate downstream components of ETHYLENE-INSENSITIVE2 (EIN2). EIN3, a downstream signalling molecule of EIN2, directly bound the <I>ORE1</I> and <I>AtNAP</I> promoters and induced their transcription. This suggests that EIN3 positively regulates leaf senescence by activating <I>ORE1</I> and <I>AtNAP</I>, previously reported as key regulators of leaf senescence. Genetic and gene expression analyses in the <I>ore1 atnap</I> double mutant revealed that ORE1 and AtNAP act in distinct and overlapping signalling pathways. Transient transactivation assays further demonstrated that ORE1 and AtNAP could activate common as well as differential NAC TF targets. Collectively, the data provide insight into an EIN2-mediated senescence signalling pathway that coordinates global gene expression during leaf senescence via a gene regulatory network involving EIN3 and senescence-associated NAC TFs.</P>
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