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Arati Ningombam,Vivek Ahluwalia,Chitra Srivastava,SureshWalia 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
This studywas aimed to evaluate the antifeedant activity of leaf extracts on third instar larvae of Tobacco Leaf Eating Caterpillar (Spodoptera litura) and bioassay guided isolation of pure compounds from Millettia pachycarpa. Plant material was extracted with two different solvents; hexane and methanol. Methanol extract was found to be active as compared to hexane extract against S. litura after 48 h andwas sequentially further partitioned between hexane, dichloromethane and butanol. DCM fraction was further subjected to column chromatography from which five fractions were obtained. Among different CC fractions, fraction 2 (FR 2) showed highest activity (AI50 227.12 ppm) and pure compound, lupeol, was isolated. Antifeedant activity results show that activity was comparable to natural plant based insecticide azadirachtin and this plant can be further explored for its use as natural pesticide.
Singh, Yanglem Herojit,Sharma, Susheel Kumar,Sinha, Bireswar,Baranwal, Virendra Kumar,Singh, N. Bidyananda,Chanu, Ngathem Taibangnganbi,Roy, Subhra S.,Ansari, Meraj A.,Ningombam, Arati,Devi, Ph. Sobit The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.6
The genetic variability of 'Candidatus Liberibacter asiaticus' (CLas) population associated with huanglongbing (HLB) disease of citrus in North Eastern (NE) region of India, a geographically locked region, and home for the diversity of many citrus species was analyzed on the basis of tandem repeat numbers (TRN) in variable CLIBASIA_01645 genomic loci. Fifty-five CLas strains sampled from different groves of NE Hill (NEH) region of India were in single amplicon group, but there was remarkable genetic variability in TRNs. The TRN in HLB-associated CLas strains varied from 0-21 and two novel repeat motifs were also identified. Among the NE population of CLas, TRN5 and TRN9 were most frequent (total frequency of 36.36%) followed by TRN4 (14.55%) and TRN6, TNR7 with a frequency of 12.73% each. Class II type CLas genotypes (5 < TRN ≤ 10) had highest prevalence (frequency of 60.00%) in the samples characterized in present study. Class I (TRN ≤ 5) genotypes were second highest prevalent (29.09%) in the NEH region. Further analysis of genetic diversity parameters using Nei's measure (H value) indicated wide genetic diversity in the CLas strains of NE India (H value of 0.58-0.86). Manipur CLas strains had highest genetic variability (0.86) as compared to Eastern, Southern and Central India. The R10 values (TRN ≤ 10/TRN > 10) of NE CLas population was 10.43 (73/7), higher from other regions of India. Present study conclusively reported the occurrence of high genetic variability in TRN of CLas population in North East Indian citrus groves which have evolved to adapt to the specific ecological niche.
Yanglem Herojit Singh,Susheel Kumar Sharma,Bireswar Sinha,Virendra Kumar Baranwal,N. Bidyananda Singh,Ngathem Taibangnganbi Chanu,Subhra S. Roy,Meraj A. Ansari,Arati Ningombam,Ph. Sobita Devi,Ashis Ku 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.6
The genetic variability of ‘Candidatus Liberibacter asiaticus’ (CLas) population associated with huanglongbing (HLB) disease of citrus in North Eastern (NE) region of India, a geographically locked region, and home for the diversity of many citrus species was analyzed on the basis of tandem repeat numbers (TRN) in variable CLIBASIA_01645 genomic loci. Fifty-five CLas strains sampled from different groves of NE Hill (NEH) region of India were in single amplicon group, but there was remarkable genetic variability in TRNs. The TRN in HLB-associated CLas strains varied from 0-21 and two novel repeat motifs were also identified. Among the NE population of CLas, TRN5 and TRN9 were most frequent (total frequency of 36.36%) followed by TRN4 (14.55%) and TRN6, TNR7 with a frequency of 12.73% each. Class II type CLas genotypes (5 < TRN ≤ 10) had highest prevalence (frequency of 60.00%) in the samples characterized in present study. Class I (TRN ≤ 5) genotypes were second highest prevalent (29.09%) in the NEH region. Further analysis of genetic diversity parameters using Nei’s measure (H value) indicated wide genetic diversity in the CLas strains of NE India (H value of 0.58-0.86). Manipur CLas strains had highest genetic variability (0.86) as compared to Eastern, Southern and Central India. The R10 values (TRN ≤ 10/TRN > 10) of NE CLas population was 10.43 (73/7), higher from other regions of India. Present study conclusively reported the occurrence of high genetic variability in TRN of CLas population in North East Indian citrus groves which have evolved to adapt to the specific ecological niche.