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      • Quantification of the digestive proteases and lipase of Schizodactylus monstrosus after the treatment of an organophosphorus insecticide, phosphamidon.

        Chanda, Subhra,Roy, S. 한국곤충학회 1986 Korean journal of entomology Vol.16 No.1

        This communication presents the results of an experimental study with phosphamidon on the digestive proteases and lipase of Schizodactytus monstrosus. The enzymes estimated are the protease, trypsin, chymotrypsin and lipase in the gut lumen and gut tissue of the insect, Both the treated and control females show significantly greater activity than male individuals. The enzymes exhibit varying results following the treatments with phosphamidon which depends upon the exposure-time. All the enzymes of both sexes show optimal activities in the treated specimens after 6 hours of treatment with phosphamidon. Although the activity of each enzyme increased in each of the gut fraction (fore, mid and hind) of both sexes yet some quantitative differences among them have been noticed. Gradual and significant declining effects have also been found after 12 hrs., 18 hrs. and 24 hrs. of treatment. The activities observed after 24 hours exposure of phosphamidon treatment appeared significantly low when compared to control. The most interesting observation is that this is true in case of all enzymes irrespective of sex and gut fraction. The physiological significance of these results are discussed in relation to the control of enzyme secretion and synthesis.

      • SCIEKCI등재

        Genetic Variability Based on Tandem Repeat Numbers in a Genomic Locus of 'Candidatus Liberibacter asiaticus' Prevalent in North East India

        Singh, Yanglem Herojit,Sharma, Susheel Kumar,Sinha, Bireswar,Baranwal, Virendra Kumar,Singh, N. Bidyananda,Chanu, Ngathem Taibangnganbi,Roy, Subhra S.,Ansari, Meraj A.,Ningombam, Arati,Devi, Ph. Sobit The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.6

        The genetic variability of 'Candidatus Liberibacter asiaticus' (CLas) population associated with huanglongbing (HLB) disease of citrus in North Eastern (NE) region of India, a geographically locked region, and home for the diversity of many citrus species was analyzed on the basis of tandem repeat numbers (TRN) in variable CLIBASIA_01645 genomic loci. Fifty-five CLas strains sampled from different groves of NE Hill (NEH) region of India were in single amplicon group, but there was remarkable genetic variability in TRNs. The TRN in HLB-associated CLas strains varied from 0-21 and two novel repeat motifs were also identified. Among the NE population of CLas, TRN5 and TRN9 were most frequent (total frequency of 36.36%) followed by TRN4 (14.55%) and TRN6, TNR7 with a frequency of 12.73% each. Class II type CLas genotypes (5 < TRN ≤ 10) had highest prevalence (frequency of 60.00%) in the samples characterized in present study. Class I (TRN ≤ 5) genotypes were second highest prevalent (29.09%) in the NEH region. Further analysis of genetic diversity parameters using Nei's measure (H value) indicated wide genetic diversity in the CLas strains of NE India (H value of 0.58-0.86). Manipur CLas strains had highest genetic variability (0.86) as compared to Eastern, Southern and Central India. The R10 values (TRN ≤ 10/TRN > 10) of NE CLas population was 10.43 (73/7), higher from other regions of India. Present study conclusively reported the occurrence of high genetic variability in TRN of CLas population in North East Indian citrus groves which have evolved to adapt to the specific ecological niche.

      • KCI등재

        Genetic Variability Based on Tandem Repeat Numbers in a Genomic Locus of ‘Candidatus Liberibacter asiaticus’ Prevalent in North East India

        Yanglem Herojit Singh,Susheel Kumar Sharma,Bireswar Sinha,Virendra Kumar Baranwal,N. Bidyananda Singh,Ngathem Taibangnganbi Chanu,Subhra S. Roy,Meraj A. Ansari,Arati Ningombam,Ph. Sobita Devi,Ashis Ku 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.6

        The genetic variability of ‘Candidatus Liberibacter asiaticus’ (CLas) population associated with huanglongbing (HLB) disease of citrus in North Eastern (NE) region of India, a geographically locked region, and home for the diversity of many citrus species was analyzed on the basis of tandem repeat numbers (TRN) in variable CLIBASIA_01645 genomic loci. Fifty-five CLas strains sampled from different groves of NE Hill (NEH) region of India were in single amplicon group, but there was remarkable genetic variability in TRNs. The TRN in HLB-associated CLas strains varied from 0-21 and two novel repeat motifs were also identified. Among the NE population of CLas, TRN5 and TRN9 were most frequent (total frequency of 36.36%) followed by TRN4 (14.55%) and TRN6, TNR7 with a frequency of 12.73% each. Class II type CLas genotypes (5 < TRN ≤ 10) had highest prevalence (frequency of 60.00%) in the samples characterized in present study. Class I (TRN ≤ 5) genotypes were second highest prevalent (29.09%) in the NEH region. Further analysis of genetic diversity parameters using Nei’s measure (H value) indicated wide genetic diversity in the CLas strains of NE India (H value of 0.58-0.86). Manipur CLas strains had highest genetic variability (0.86) as compared to Eastern, Southern and Central India. The R10 values (TRN ≤ 10/TRN > 10) of NE CLas population was 10.43 (73/7), higher from other regions of India. Present study conclusively reported the occurrence of high genetic variability in TRN of CLas population in North East Indian citrus groves which have evolved to adapt to the specific ecological niche.

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