Diatomaceous earth를 이용한 경제적이고 효율높은 homemade PCR purification column의 제조 및 평가

이동호,이승관,이창규,조경진,최명재,이국성 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

In recent years the amount of sequencing has increased tremendously, especially due to the introduction of automated DNA sequencing. PCR is principal method for the synthesis of templates for DNA sequencing. Consequently, the need for PCR cleanup for template preparation has increased. Already, many PCR purification kits are marketed. This DNA purification kits uses most glass membrane or silica gel, So, can do rapidly efficient purification by simple miniprep. But, efficiency of purification kits is different according to manufacturer and is very expensive. Therefore, we compare the homemade Diatomaceous earth column with major manufacturer's spin column methods for DNA purification. As a result, we found that the homemade Diatomaceous earth column method resulted in equal or better yields relative to the commercial purification kits.

아연계 탈황제의 장기연속 반응성 연구

박노국,이종욱,이승철,이종대,류시옥,이태진 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.2

The zinc titanate sorbents(ZTG40) were prepared and the tests for durability and attrition resistance were carried out to develop the highly effective desulfurization sorbents. In the attrition tests for ZTG40 the results showed that the attrition resistance was 94.08% and that the sulfur capacity was maintained above 15g S/100g sorbent throughout the 100-cycle test, its maximum being 23.05g S/100g sorbent. As compared to sorbents developed by the developed countries, ZTG40 showed superior performances. In the advanced countries 15g S/100g sorbent is a yardstick of excellency for the sulfur capacity of desulfurization sorbents. Therefore, ZTG40 developed in this study has the desirable physical and chemical properties for fluidized desulfurization of coal gases.

變煥法에 의한 設計渴水量의 誘導

李淳赫,朴鐘國,孟昇辰 충북대학교 농업과학기술연구소 1994 農業科學硏究 Vol.11 No.2

Derivation of reasonable design low flows was attempted by comparative analysis of design low flows derived by Power and SMEMAX transformations for the normalization of skewed distribution in the four watershed of main river basins in Korea. The results were analyzed and summarized as follows. 1. Basic statistics of annual low flows for the selected watersheds were calculated by using Power and SMEMAX transformations. 2. Power transformation has found to be the best for the normalization of skewed distribution among others including Log, Squareroot and SMEMAX transformations. 3. Design low flows for the seleted watershed were derived by the Power and SMEMAX transformations. 4. Judging by the relative suitablities for the Power and SMEMAX transformations, it was found that design low flows using two methods are closer to the observed data within 10 years of the return period, and those of Power transformation can be acknowledged as a reasonable one in comparison with SMEMAX transformation over 10 years of the return period.

퍼지 역문제 알고리즘을 이용한 입력값 수정치 분석에 관한 연구

이승민,성국경 공주영상정보대학 1998 논문집 Vol.5 No.-

퍼지수 요소를 기존의 크리스프형 퍼지관계식에 도입함으로써 「약간」, 「조금더」등과 같은 구간(폭) 표현을 양으로 다루는 것이 가능하고, 정보의 애매성을 적극적으로 활용할 수 있다. 이것에 인해, 퍼지관계식을 공학적인 시스템에 한정하지 않고, 사회 시스템, 의사결정 시스템등과 같은 애매한 요소를 포함한 계통에 쉽게 적용할 수 있다. 또, 복잡한 계산처리를 요구하는 경우에도, 퍼지수의 연산을 크리스프값의 연산으로 치환해서, 기존의 퍼지관계식과 같은 연산방법에 의해서 해를 쉽게 구할 수 있다. 본 논문에서 제안한 퍼지수 요소를 갖는 퍼지관계식의 해법을 다양한 사례에 적용하여 그 유효성을 검증할 필요가 있다.

송전선로 보호계전기 정정값 진단 시스템

이승재,김국진,최면송,강상희 明知大學校 産業技術硏究所 2000 産業技術硏究所論文集 Vol.19 No.-

In the point of view a protective relay, the selectivity and sensitivity is very important in its performance. The correct setting of the relay parameter is required. The verification of accurate setting for the protective relay is very difficult before a actual fault occur. In this paper, we proposed the diagnosis expert system as a method to verify the correctness of the relay setting. The developed system proved effectiveness through the tests on the real systems.

21C 부산체육의 활성화를 위한 시설 및 프로그램 연구

이상봉,김용재,서국웅,양점홍 釜山大學校 附設 體育科學硏究所 1996 體育科學硏究所 論文集 Vol.12 No.-

This research investigated Institution and Program for the Development of the Pusan Sports in 21C by examining literatures. Conclusiona from the results as follows. 1. We should provide a long period propulsion project in three stage with planning, diffusion and development stage for the bring development and diffusion of Program. 2. We should establish a specialty research institution, the exclusive responsibility of program's development and research. 3. We should provide various participation programs not that for an elite athlete but that for all nation. 4. Enlarge the same taste club and we must support with various administration and financial. 5. Cultivate a specialty leader and we must take interested in the study and training with continual.

공개키 기반 신뢰성 강화 인증시스템 설계

성국경,이봉근,윤성일 공주영상정보대학 2001 논문집 Vol.8 No.-

본 논문에서는 전자 상거래를 위해 암호 기술의 확장성 측면에서 우수한 공개키 기반 하부 구조에서의 공개키 인증 기술을 중심으로 분석하였고, 인증기관에서 발급하고 있는 인증서의 기능을 보강함으로써 인증기관의 신뢰도를 높일 수 있는 신뢰성을 강화한 인증 시스템을 설계하였다. 공개키 기반 인증 기술은 전자 상거래 서비스에서 필요한 신분 인증, 거래 내역 인증 및 지불 처리 인증에 사용하는 기반 기술이다. 이는 공개키를 보증하는 기반 기술과 인증서의 안전한 사용 기술 등이 필요하며 전자 상거래를 실현하기 위한 필수적인 선결 사항이다. 인터넷이라는 불안전한 개발형 전산망 구조에서 안전한 전자 상거래를 제공하려면 향후 국제적인 인증 서비스와의 연동을 위한 표준화된 인증 기술의 적용이 중요하다. 현재까지 정보의 공유라는 원칙에서 발전해 온 인터넷은 앞으로 전자상거래나 전자화폐 등이 인터넷상에 등장함으로 인해 정보의 보호라는 새로운 원칙을 바탕으로 발전해 나아갈 것이다. 이러한 정보보호시대에 살아 남기 위해서는 종속적이 아닌 독자적인 암호화 기술과 데이터 인증기술의 개발이 절실하게 필요한데, 여기에 인증국의 중요성이 부각된다. 현재 암호화 기술은 어느 정도 신뢰를 줄 만큼 발전해 있지만 아직까지 데이터 인증을 위한 기반구조가 명확히 성립되어 있지 않은 상태이다. 앞으로 인증국을 기반으로 한 공개키 기반구조 확립이 빠른 시일 안에 이루어 져야 할 것이다.

안면 마비가 동반된 톨로사 헌트 증후군

강성진,김광수,이지현,김종국,김민정 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1

A 21-year-old woman developed painful left third, fourth and sixth nerve palsy. The retro-orbital pain and ophthalmoplegia were improved after steroid pulse therapy. After six weeks, during took steroid by orally left facial weakness developed. Neurologic examination showed left peripheral seventh cranial nerve palsy. The Tolosa-Hunt syndrome associated with facial palsy is rare condition. So we report a case of Tolosa-Hunt syndrome preceded by ipsilateral facial palsy

코크스 오븐에서 에너지 효율 향상을 위한 통합연소진단시스템 개발

이지수,이용국,이성우,심재우 한국공업화학회 2004 응용화학 Vol.8 No.1

In making coke through carbonization process, it is possible to produce the required high quality coke in blast furnace and reduce the cost of energy as well as increase the life of coke oven if we maintain the desired coke oven temperature by means of optimal combustion management (CM). In this study, hybrid combustion diagnostic expert system (HCDES) was developed through analyzing the trend and temperature distribution of flues in the oven and combining the domain expert's knowledge. The model also considered and incorporated the characteristics and the structure of the oven. In developing the diagnostic system, a general and accessible expert language, CLIPS (C Language Integrated Production System) was used.

신부전이 동반된 당뇨병 환자에서 발생된 침습성 모균증 : 수술과 Liposomal amphotericin B 및 GM-CSF 병합 요법에 의한 성공적인 치험 1예

이원영,오기원,임국희,장재혁,이동건,최정현,강무일,신완식,차봉연,이광우,손호영,강성구 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.4

저자들은 신부전이 동반된 당뇨병 환자에서 발생된 부비동형 모균증에 대하여 수술과 함께 liposomal amphotericin B 및 GM-CSF의 복합치료를 하여 성공적으로 치료한 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Mucormycosis (zygomycosis) primarily affects diabetic or immunocompromised patients and typically progresses rapidly, necessitating surgical excision and antifungal therapy with amphotericin B. Large doses of amphotericin B are needed for cure, but it has the risk of causing significant renal toxicity. The recent development of liposomal amphotericin B allows antifungal therapy to be administered with potentially improved efficacy and reduced nephrotoxicity. We have experienced a case of paranasal mucormycosis successfully treated with surgery, liposomal amphotericin B and GM-CSF. A 59-year-old male suffering from diabetes mellitus for 6 years was admitted with pain at left maxillary area. He was diagnosed as mucormycosis after cytologic exam on the necrotic nasal mucosa, which showed typical hyphae. He have had diabetic nephropathy with macroproteinuria and had rapidly rising serum creatinine levels with the amphotericin B treatment: creatinine levels reverted to basal level with the use of liposomal amphotericin B. Despite surgical excision and continued antifungal therapy, his infection was not effectively controlled. Therefore, GM-CSF was administered additionally to improve phagocytic activity of leukocytes. He was finally cured after receiving a combination of aggressive surgery, liposomal amphotericin B and GM-CSF. To our knowledge, this is the first detailed clinical description of the treatment of mucormycosis with liposomal amphotericin B in Korea.