Three-Dimensional Porous Collagen/Chitosan Complex Sponge for Tissue Engineering

Kim, Sung Eun,Cho, Yong Woo,Kang, Eun Jung,Kwon, Ick Chan,Lee, Eunhee Bae,Kim, Jung Hyun,Chung, Hesson,Jeong, Seo Young The Korean Fiber Society 2001 Fibers and polymers Vol.2 No.2

A three-dimensional, porous collagen/chitosan complex sponge was prepared to closely simulate basic extracellular matrix (ECM) constitutes, collagen and glycosaminoglycan. The complex sponge was prepared by a lyophilization method and had the regular network with highly porous structure, suitable for cell adhesion and growth. The pores were well interconnected, and their distribution was fairly homogeneous. The complex sponge was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to increase its boilogical stability and enhance its mechanical properties. The crosslinking medium has a great effect on the inner structure of the sponge. The homogeneous, porous structure of the sponge was remarkably collapsed in an aqueous crosslinking medium. However, the morphology of the sponge remained almost intact in a water/ethanol mixture crosslinking milieu. Mechanical properties of the collagen/chitosan sponge were significantly enhanced by EDC-mediated crosslinking. The potential of the sponge as a scaffold for tissue engineering was investigated using a Chinese hamster ovary cell (CHO-K1) line.

만성 위염전 2 례

박찬규,이동욱,남성진,김추성,조성래,구본천,박영재,허규찬 啓明大學校 醫科大學 1997 계명의대학술지 Vol.16 No.3

위염전은 위의 비정상적인 회전으로 생기는데 급성증에서는 진단이 늦어질 경우 치명적일 수도 있으나, 만성증의 경우는 비특이적 위장관 중상을 나타내며, 치료되지 않을 경우 수년 동안 증상이 지속될 수도 있다. 최근 저자들은 소화불량, 식후 상복부 동통을 주소로 내원한 환자에서 만성위염전 2 례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Gastric volvulus is an abnormal rotation of the stomach. Gastric volvulus may present as an emergency or as a chronic condition. Acute volvulus si potentially lethal if unrecognized, while a chronic volvulus may cause symptoms for years if not treated. Gastric volvulus may be more common than previously estimated. Approximately 700 cases have thus far been documented. In patients with vague upper abdominal pain, bloating, vomiting, early satiety and weight loss, chronic gastric volvulus are reported and the related literature reviewed. The clinical presentation, diagnosis, and treatment are treatment are discussed. Features of acute and chronic volvulus are compared.

폴리에스테르 섬유 감량시스템용 프로세서 타이트레이터 개발

조진호,이흥락,이종현,김명남,구성모,김무영,진경찬,유병희,강민수,김국진 경북대학교 센서기술연구소 1993 연차보고서 Vol.1993 No.-

폴리에스테르 섬유 감량시스템의 제어를 위해서는 처리조 내에서 감량되는 섬유가 목표 감량치에 도달했는지를 판단해 주는 장치가 필요하다. 본 연구에서는 가성소다용액의 초농도 및 섬유 감량 정도의 정확한 측정과 감량 종료시간의 산출 및 이에 따른 종료신호를 발생할 수 있는 폴리에스테르 섬유 감량기 전용의 자동적정장치를 개발하였다. To control caustic soda treatment system for the polyester fabric reduction, we need the device to determine that the fabric has been arrived target reduction value. In this study, we develope an automatic process titrator for the caustic soda treatment system. Major functions of the target titrating system are accurate measurements of the caustic soda concentration and then producing the end point signal for caustic soda treatment system.

술후 Morphine과 Butorphanol의 지속적 정주 효과 비교

조상선,안정찬,이상근,한인숙,장사충 日新基督病院 1997 日新基督病院論文集 Vol.12 No.-

예혼합 난류유동장내 점화핵의 난류화염두께 및 난류화염속도의 예측

조성찬,문희장 한국항공대학교 항공우주산업기술연구소 2003 航空宇宙産業技術硏究所 硏究誌 Vol.13 No.-

The propagation of a flame front subjected in a given intensity of turbulent flow is numerically investigated using the presumed PDF methods. The main characteristics of a turbulent flame thickness is also calculated in order to capture the basic behavior of a turbulent flame. It was found that the proposed models can achieve the prediction of these fundamental properties of turbulent flame, and demonstrate the capacity of probability density function based statistical model. A spherical coordinate is used to simulate the on growing kernel characteristics at the early stage of spark event. The result shown for the turbulent flame thickness allowed us that this method have the potential and can be extended to low Damko¨hler number regime. A more concrete analysis is expected in the future with a more elaborated chemical reaction model.

담즙산류의 항스트레스 효능에 관한 연구

조태순,이종찬,조서익,이선미 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

This study was done to investigate whether cholic acid derivatives have anti-stress activity in various stress models. Two cholic acid derivatives, ursodeoxycholic acid (UDCA) and tauroursodeoxycholic acid (TUDCA), were used. Physical, psychological, chemical and environmental stress models were performed. Adrenal weight, serum glucose levels and ALP activity were elevated in restraint stress model, but this elevation was prevented by UDCA treatment. Moreover, UDCA and TUDCA inhibited exploratory and spontaneous movements in oscillation stress model. In alcohol-induced stress model, TUDCA improved rotarod performance. UDCA and TUDCA significantly reduced the involution of lymphoid organs and the increment of WBC counts in cold stress model. These findings suggest that cholic acid derivatives have antistress effects in various stress models.

산포도와 머루중의 Polyphenol Oxidase에 관한 연구¹

성찬기,박희중,임흥빈,조성희 中央大學校 基礎科學硏究所 1997 基礎科學硏究所 論文集 Vol.11 No.-

Polyphenol oxidase was partially purified from fresh vitis flexuosa and vitis amurensis and its characteristics were studied. the experimental results were summarized as follows. 1.The enzyme activities were increased during the fruit developement gradually in two sample. 2.The optimum pH and temperature for activity were 5.5 and 30℃, respectively. 3.The enzyme was active with o-phenols and trihydroxyphenol, but inactive toward m-diphenols and monophenols. 4.The ?? value of the enzymes was 15.4 mM in vitis flexuosa and 12.5 mM in vitis amurensis with catechol as substrate. 5.Inhibitors sudies indicated that sodium diethyl-dithiocarbamate, L-cysteine, sodium bisulfite, L-ascorbic acid and 2-mercaptoeyhanol were the most potent. 6.The enzyme activity increament by ?? was much better than those by the other metal ions.

MSGC형 센서를 위한 제어시스템 및 영상 획득 S/W 개발

조진호,진경찬,채승표,박영식,김명남 경북대학교 센서기술연구소 1997 연차보고서 Vol.1997 No.-

악성 혈액질환에서 성공적인 동종골수이식 후 숙주 기질 미세환경의 구축

조상희,이제중,남찬은,최경상,정익주,이일권,김진희,박종태,김형준 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.1

인체의 골수는 간엽모세포를 함유하고 있으며 이들은 골수미세환경의 주된 세포들로 분화가 가능하여 조혈기능을 지지한다. 본 연구에서는 성별이 다른 동종조혈모세포이식 환경에서 조혈모세포의 완전 생착을 보이고, 이식 후 1년에서 8년이 지난 11예의 재생불량성 빈혈 및 백혈병 환자들을 대상으로 하여 골수에서 MSC를 분리하고 체외 확장을 통해 배양된 MSC에서 X 염색체 탐식자를 이용한 FISH 및 microsatellite polymorphism PCR 기법으로 그 기원을 확인하였다. 그 결과 조혈모세포는 완전히 공여자 기원으로 대치되었음에도 불구하고 MSC는 모두 수여자 기원임을 알 수 있어, 동종조혈모세포이식에서 미세환경의 구축은 수여자의 자가 생산에 의한 골수 간질세포에 의한 것으로 생각된다. Background: Human bone marrow (BM) contains mesenchymal stem cells (MSC) that can differentiate into various cells of mesenchymal origin. It remains a matter of controversy whether donor-derived stromal cells are capable of engraftment following hematopoietic stem cell transplantation (HSCT) or not. Methods: To determine if donor-derived stromal cells are transferred to the recipients of allogeneic HSCT, we investigated the characterization of MSC in 11 patients 1 to 8 years after sex mis-matched allogeneic HSCT in severe aplastic anemia and leukemia. Results: All patients had complete engraftment with donor- derived stem cells as shown by detection of donor type DNA in peripheral blood mononuclear cells. Following culture, MSC showed the expression of SH2 and SH4, but none of the hematopoietic markers of CD14, CD34, or CD45. MSC which can be differentiated to osteogenic lineage showed the genotype of recipient completely using FISH or PCR analysis. Conclusion: This study confirmed that MSC isolated from recipients of allogeneic HSCT in severe aplastic anemia and leukemia are not of donor genotype despite of full hematopoietic engraftment with donor type. Donor cells did not contribute to reconstitute the marrow microenvironment.

정보통신산업 생산성 변화의 산업간 파급효과 분석

조영상,이정동,박찬수,김태유 한국생산성학회 2002 生産性論集 Vol.16 No.3

The Korean ICT industry is evolving at a dramatic speed, increasing its importance in the overall economy and producing numerous externalities on the other industries. The purpose of this srsearch is to investigate the productivity growth of Korean ICT industry and its effects on the other industries and to derive policy implications. For this purpose we perform inter-industry analysis. Inter-industry analysis is conducted to investigate how close co-relationship exist between ICT industry and other indrstries and to see how much ICT industry affects other industries. Our estimates of the productivity growth in the industry and its contribution to the overall korean Economy suggest the direct contribution of the ICT industry is significant, but its spillovers to the other industries are not so impressive. This implies that the link between ICT industry and other industries is weak and that most of the ICT products are used as final products and not as intermediate goods. We also find the high extent of import penetration in Korean ICT industry to be one of its major problems.