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사람 치은모세포의 세포주기에 미치는 light-emitting diode 조사 효과
Jung Hye Kim,Youn Young Jang,Kwon Jung,Mi Kyeong Ko,Mi Sun Jang,Eun Byul Kook,Min A Chong,Won Bong Lim,Chae Kwang Lim,Ok Joon Kim,Hong Ran Choi 대한구강악안면병리학회 2006 대한구강악안면병리학회지 Vol.30 No.2
It has been reported that light-emitting diodes(LED) can be used in the treatment of oral diseases. Although bio-stimulatory effects of LED irradiation such as promotes stimulation of wound healing have been well known, there are few reports about molecular mechanism associated with cell cycle by LED irradiation. The purpose of present study was to examine the molecular event in cell cycle of LED irradiation on primary human gingival fibroblast(hGF) in vitro. The source of light for irradiation was a continuous-wave LED emitting at a wavelength of 635nm, and manufactured that energy density was 5mW/cm2 on sample surface. The hGF were irradiated for 1 hour at 37℃ in 5% CO2 humidified chamber. Experimental samples were acquired at 0 (right after irradiation), 8 and 24 hour after irradiation. To investigate the molecular mechanisms associated with cell cycle, growth phase was determined by flow cytometry and mRNA expression of cyclin A, cyclin B, cyclin D1, cyclin E, cdc2, PCNA, p18, p27, p21, and p53 were determined by real time RT-PCR. Flow cytometric analysis demonstrated the percentage of cells in the G1 and S phase were decreased, but the G2 phase increased, which showed cells irradiated by LED were transitioned from S to G2 phase. For mRNA expression, cyclin B, cdc2, PCNA and p53 were increased at 0 hour after irradiation, and most of cell cycle molecules were increased at 8 hour after irradiation. At 24 hour after irradiation, cyclin A, cyclin E, PCNA and p18 were increased. Taken together, LED irradiation induced proliferation of hGF cells through transition from S to G2 phase.
Sun Young Park,Min Jung Kim,Mun Gyu Kim,Se Jin Lee,Soon Im Kim,Sang Ho Kim,Si Young Ok,Soon Im Kim 순천향대학교 순천향의학연구소 2011 Journal of Soonchunhyang Medical Science Vol.17 No.1
Objective: Topical steroids are a good option for preventing postoperative sore throat (POST). This study examined whether triamcinolone paste applied as lubricant reduces the severity of POST following laryngeal mask airway (LMA) insertion. Methods: This was a prospective, randomized, double-blind, placebo-controlled clinical trial. The study enrolled 50 American Society of Anesthesiologists (ASA) I-II patients who were between 20 and 70 years of age and scheduled for elective surgery under general anesthesia. The patients were divided randomly into two groups. Patients in the chlorhexidine group (the placebo group) were inserted with a LMA lubricated with chlorhexidine gluconate jelly, whereas patients in the triamcinolone group were inserted with a LMA lubricated with 0.1% triamcinolone acetonide paste. The patients were interviewed 1, 6, and 24 hours after the operation. The incidence and severity of POST and the incidence of cough and hoarseness were recorded. Results: The difference of the POST incidence during the 24 hours after the operation was not significant (34.8% in triamcinolone group vs. 45.5% in chlorhexidine group, P=0.381). The severity score in the triamcinolone group was significantly lower than the chlorhexidine group at 1 hour after the operation (P<0.001). No significant differences were found in the incidence of cough, hoarseness, dysphagia, nausea, or dry throat between the two groups. Conclusion: Triamcinolone paste applied as lubricant reduces the severity of POST following LMA insertion.
Jung, Sun Ok,Ro, Hyeon-Su,Kho, Byung Hoon,Shin, Yong-Beom,Kim, Min Gon,Chung, Bong Hyun WILEY-VCH Verlag 2005 Proteomics Vol.5 No.17
<P>The E7 protein produced by high-risk human papillomavirus (HPV) induces a degradation of the retinoblastoma tumor suppressor RB through direct interaction, which suggests that an inhibitor for the interaction can be a potential anticancer drug. A surface plasmon resonance (SPR) imaging-based protein array chip was developed for the high-throughput screening of inhibitor molecules targeting RB-E7 interaction. The glutathione S-transferase-fused E7 protein (GST-E7) was first layered onto a glutathionylated gold chip surface that had been designed to specifically bind to GST-fused proteins. Subsequently, a microarrayer was used to spot the hexa-histidine-tagged RB proteins (His<SUB>6</SUB>-RB) onto the GST-E7-layered gold chip surface, and the resulting SPR image was analyzed. Upon increased His<SUB>6</SUB>-RB concentration in the spotting solution, the SPR signal intensity increased proportionally, indicating that His<SUB>6</SUB>-RB bound to GST-E7 in a concentration-dependent manner. The His<SUB>6</SUB>-RB/GST-E7 interaction was challenged by spotting the His<SUB>6</SUB>-RB solution in the presence of a RB binding peptide (PepC) derived from a motif on E7. The SPR imaging data showed that PepC inhibited the His<SUB>6</SUB>-RB/GST-E7 interaction in a concentration-dependent manner. Our results show that the SPR imaging-based protein array chip can be applied to screen small molecule inhibitors that target protein-protein interaction.</P>