한국해안으로부터 Purple, Non-Sulfur Photosynthetic Bacterium, Rhodobacter sp. EGH-24의 분리 및 특성

차미선,김기한,조순자,이나은,이정은,이재동,이상준,박재림 한국환경과학회 2003 한국환경과학회지 Vol.12 No.12

A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the 47 point at west and south coast of Korea in September 2001. Separated 13 samples of changes with red color under 28~32 ℃, 3000 lux, anaerobe conditions for 7 days cultivated in basal medium. For pure isolation from 13 samples, we used agar-shake tube method (0.4 % agar) and separated 5 strains through 13-repetition test. EGH-24 and EGH-30 was identified as the same strain through the RAPD(Random Amplified Polymorphic DNA)-PCR of strain EGH-9, EGH-13, EGH-23, EGH-24, EGH-30. Four isolates cultivated in synthesis wastewater for wastewater biodegradation test. EGH-24 was selected with efficient wastwater treating strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 16S-rDNA phylogenetic analysis, EGH-24 strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.

원유의 미생물적 유화

차미선,손홍주,이건,고명선,이상준,이종근 부산대학교 환경문제연구소 1996 環境硏究報 Vol.14 No.1

Microorganisms producing bioemulsifier were isolated from the sea water in Pusan costal aera. The isolated strain which had the highest emulsification activity and stability was identified as the genus Acinetobacter from the results of morphological, cultural and biochemical tests and named Acinetobacter sp. EL-C6 for convinience. The compositions of optimum medium for emulsification of crude oil by Acinetobacter sp. EL-C6 were crude oil 2%, NH_4NO_3 0.2%, K_2HPO_4 0.01%, MgSO_4·7H_2O 1%, CaCl_2·2H_2O 0.1%, NaCl 3% and the initial pH 7.5. The cultivation for emulsification of crude oil was carried out in 500m1 shaking flask containing 100m1 of the optimum medium at 30℃. The highest emulsification was observed after 5 days. The utilization on the various hydrocarbon of the Acinetobacter sp. EL-C6 was showed that the utilization of n-alkane compounds were better than that of aromatic compounds. Among the petroleum compounds, by Acinetobacter sp. EL-06.

불소함유 그라프트 공중합체의 표면특성

차미선,소원욱,박인준,김동권,이수복,ShiYuan, Cheng 한국공업화학회 2003 응용화학 Vol.7 No.2

The graft copolymer, consisting of a flurorinated macromer(F-macromer) and a 2-(dimethyl amino)ethyl methacrylate, were prepared by marcromonomer technique and radical copolymerization. In this study, the surface propeties of the graft copolymer and its additive effect on the surface energy of polymer film were compared with the random copolymer of poly(perfluoroalkyl ethyl acrylate)-r-poly(2-dimethylamino ethyl methacrylate)(PFA-r-P(2-DMAEMA)). The contact angles of the surface modified by the graft copolymer is large than that of random copolymer with similar FA monomer content, and surface free energy of the substrate containing 0.01wt% of the graft copolymer is similar with that of 0.1wt% random copolymer surface. That is, The low-energy modification effect of the graft copolymer is better than that of the random copolymer.

불소 함유 양성고분자의 분리와 특성

차미선,소원욱,박인준,이수복 한국공업화학회 2003 응용화학 Vol.7 No.1

We describe the synthesis and characterization of amphoteric poly(acrylate), consisting of a F-marcromer and a 2-(dimethyl amino)ethyl methacrylate (2-DMAEMA). The amphoteric polymer were prepared by marcromonomer technique and radical copolymerization. After copolymerization, the resultant reaction solution was purified by silica column. In this study, separation of amphoteric poly(acrylate)s and characterization by InfraRed Spectroscopy, Gel Permeation Chromatography, Polarized Optical Microscopy, and Differential Scanning Calorimeter were described. The IR traces showed that the amphoteric poly(acrylate) was separated successfully and the characterisitic peaks of both hydrophobic F-macromer and hydrophilic polymer of p(2-DMAEMA). The amphoteric poly(acrylate) have number average molecular weights(Mw) about 38.000 by GPC.

대학생의 노인복지에 대한 인식도

차병준,김무룡,김기열,차경미,김귀희,김현실,서인선,임상규,이순자,위광복,남철현 慶山大學校 保健福祉硏究所 1999 保健福祉硏究 Vol.2 No.-

The purpose of this study is to grasp the change of consciousness of young people in order to seek new approach of policy for aged era. 1,200 students attending collages and universities in three small or medium sized cities were inquired for 2 months, from October 1 to November 30, 1996. The result is as follows. 1. The characteristic of the respondents of this survey is that they include 72.1% of women, 40.4% of people aged between 20 to 21, and 49.1% of people who do not have any religion. Those who are from big cities and those who are form farming villages or fishing village occupy equally 40.2%, those who reside in that cities for a long period 49.0%, and those who live with their family 60.9%. 2. The family feature of the subject is that 31.5% of them are those whose father is the age of 55 and more than 55, 10% of them are those whose mothers age is so, 33.1% of them have 5 family members, 29.5% of them have the family income of 1,000,000 to 1,490,000 won, 83.9% of them are from middle class, 47.7% of them are the people whose grand parents have passed away, 70.4% of them live apart from their grand parents, and 60.9% of them are second child. 3. 64.0% of males, 70% of respondents who are aged over 24, 58.1% of respondents who are from rural communities, 62.4% of respondents who live in rural area, and 58.3% of respondents who live alone, prefer to live with grand parents, marked higher rate than other groups. The groups whose father and mother are the age of more than 55 marked higher rate of 55.9% and 58% each than any other groups(p<0.05). The groups whose family member is less than three, whose grand parents both have passed away in middle class, who live with their grand parents, and who are second children, give more positive answers than any other groups. 4. Those who are older, who are buddhists, who are from rural area or live in rural area, whose family is small, whose family income is small, who are from lower class and whose grand parents are still alive, shows preference to support their parents after marriage. 5. Females(89.4%), those who are from small or medium sized cities(89%), who live with their family(85.6%), whose father is the age of 55 and more than 55, whose mother is the age of 54 and less than 54, whose family member is 6, who are from middle class, whose grandparents are still alive and who are the eldest child in their family, give more response that they prefer to live apart from their children in their old age than any other groups. 6. What elderly people need most right now is past time(42.3%), and the respondents whose age is 20 to 21, who are buddhists, who are from middle sized cities and live alone, whose parents are the age of more than 55, who have small family income, who live with their grand parents and who are second children show higher level than any other groups. 7. 76.8% of respondents answer that they do not need their parent's fortune, marked still higher rate than the opposite answer. Those who are older, who are buddhists, who are from big cities, who reside in rural area, who live alone and have smaller family, who are in bad family economic conditions and who live with grand parents present higher rate of positive response. 8. 59.3% of respondents reply that they think about death from time to time, and those who are female, who are in the age of 20 to 21, who are christian, who live with their family, whose siblings or other family members offer their education expenses show higher rate than any other groups. 9. 92.3% of respondents answer that the budget for welfare facility for elderly people is very small. The younger the respondents are and the worse economic condition they have among those who live in big cities, the more answered that. 10. 50% of respondents think social security should provide for their old age, and 42.8% of them believe they themselves provide against it. The respondence that social security ought provide for it appeared more among those whose age are over 24, who are female, who reside in rural area, who are christian, who are in bad economic condition, and who ever used medical institutions in recent one month, marked higher level than other groups. 11. The adequate time of retirement is 59.97±5.60 for public officers, the highest and 59.28±6.35 for teachers, the next. 12. 35.2% of respondents reply that the adequate budget for the old age after their retirement at the age of 60 is 220 million won, showed the highest level of respondence. 13. The factors which affect on the old people in the charged welfare facilities are sex(p<0.01), the type of housing(p<0.05) and whole family income(p<0.05). 14. The factors which affect on remarriage are sex(p<0.05) and religion(p<0.05). For varying old population and expanded welfare service for them, government and relevant authority should give more attention to secure the budget, establish effective plans to expand various program and the facilities for elderly people in order that elderly people spend the rest of life time more happily and more fruitfully. To do that, each college and university should open Health courses, and educate and inform about health and welfare.

불소함유 지문방지제 합성

차미선,범정철,하종욱,박인준,소원욱,이수복 한국공업화학회 2004 응용화학 Vol.8 No.2

The anti-fingerprint coating agents, consist of a fluorinated macromer(F-macromer) were prepared by marcromonomer technique and hydrosilation reaction. The products were characterized by ¹H-NMR, differential scanning calorimetry. The surface properties of the anti-fingerprint coating agents were investigated by the contact and sliding angle measurement. The modified surface with the anti-fingerprint coating agents showed the extremely low surface free energy of a 10.09 dyn/cm and the sliding angle was smaller than that of commercial products. That is, The synthesised coating agents can be the good candidate for the water and oil repellent, soil release and anti -fingerprint application coating agent.

불소 함유 양성고분자의 분리와 특성

차미선,소원욱,박인준,이수복 한국공업화학회 2004 응용화학 Vol.8 No.1

We describe the synthesis and characterization of amphoteric poly(acrylate), consisting of the F-marcromer and a 2-(dimethyl amino)ethyl methacrylate (2-DMAEMS) The amphoteric polymer were prepared by marcromonomer technique and radical copolymerization. After copolymerization, the resultant rection solution was purified by silica column. In this study, separation of resultant reaction sloution was purified by silica column. In this study, separation of amphoteric poly(acrylate)s and characterization by InfraRed Spectroscopy, Gel Permeation Charomatography. The IR traces showed that the amphoteric poly(acrylate) was separated successfully polymr of p(2-DMAEMA). The amphoteric poly(acrylate) have number average molecular weights(Mw) about 38,000 by GPC.

Pseudomonas sp. EL-G527에 의한 환경친화성 생물계면활성제의 생산최적조건

차미선,임은경,이근희,조순자,손흥주,이상준 한국환경과학회 2002 한국환경과학회지 Vol.11 No.3

A biosurfactant-producing microorganism was isolated from activated sludge by enrichment culture when grown on a minimal salt medium containing n-hexadecane as a sole carbon source. This microorganism was identified as Pseudomonas sp. and it was named Pseudomonas sp. EL-G527. It's optimal culture condition is 2% n-hexadecane, 0.2% NH_4NO_3, 0.3% KH_2PO_4, 0.3% K_2HPO_4, 0.02% MgSO_4·7H_2O, 0.0025% CaCl_2·6H_2O, 0.0015% FeSO_4·7H_2O in 1ℓ distilled water and initial pH 7.0. Cultivation was initiated with a 2% inoculum obtained from starter cultures grown in 30 ㎖ of the same medium in 250 ㎖ flask. They were cultivated at 30 ℃ in reciprocal shaking incubator and the highest biosurfactant production was observed after 4 days.

피브린 고분자와 반원상 연골세포를 이용한 손상된 가토 반원상 연골의 재생

손선미,이재선,최차용,박종용,김병수,박정호 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

국내산 무화과를 이용한 와인 제조 및 품질 개선 효과

정미란,차정단,윤순일,한종현,이영은 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.1

Fig(Ficus carica L.) which belongs to Moraceae family has been used as digestion promoter, cure for ulcerative inflammation and eruption in Korea. Figs have very short preservation period and can be kept only 1 week even in the refrigeration system. The possibility of wine-making from Korean figs was tested by analyzing the fermentation characteristics, proximate analysis and free sugar contents of wine made of slices with 20% sugar addition. Ethanol fermentation of the figs by Saccharomyces cerevisiae was done rapidly. The ethanol contents, pH, total acidity and sugar content were 12.1%, 3.91, 0.42% and 9.9 °Brix, respectively. The results from the sensory evaluation were as follows. The wine made from the sliced figs were better than that from fig juices in their sensory scores. The wine made with frozen figs was not different from that made with fresh ones. To improve the quality of wine, the addition of dried leaf powder in the concentration of 100 ppm were tried and found it was superior to the one made without leaf.