Comparative analysis of bovine maternal corpus luteum microRNAs with aberrant and normal developed cloned fetus at late gestation

Xiaohu Su,Shenyuan Wang,Guangqi Gao,Xinyu Zho,Lidong Han,Guanghua Su,Jiaqi Zhang,Wanfu Bai,Xiuying Wang,Guangpeng Li,Li Zhang 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background The development efficiency of cloned cattle is extremely low (< 5%), most of them were aborted at late gestation. Based on our previous studies, some recipient cows with a cloned fetus would present as engorged uterine vessels and enlarged umbilical vessels randomly. Abortion involves both maternal and fetal factors. Objective Our aim was to explore this phenomenon by microRNAs expression profile analysis of maternal corpus luteum (CL), which was related to pregnancy maintenance. Methods The present study provided the comparison of maternal CL miRNAs expression of abnormally and normally developed cloned bovine fetus at late gestation (~ 210 days) using RNA-Seq technology. Results We selected two abnormally pregnant cows (abnormal group, AG) and three normally pregnant cows (normal group, NG) and acquired valid reads of 9317,261–12,327,185 (~ 84.53–91.28%) from five libraries. In total, we identified 981 conserved miRNAs and 223 novel miRNAs. 1052 miRNAs were co-expressed, 124 miRNAs were uniquely expressed in AG, and 93 miRNAs were uniquely expressed in the NG. Compared with NG, 11 were significantly overexpressed, and 22 were downregulated (p < 0.05) at AG among 1052 co-expressed miRNAs. The differentially expressed miRNAs-targeted genes were further analyzed by Gene Ontology and KEGG pathway analysis. Notably, the steroid biosynthesis pathway was a significantly enriched term (p < 0.01), which may affect the secretion of progesterone. Conclusion Our research suggested that abnormal miRNAs expression of bovine maternal CL may affect the pregnant status at late gestation.

Occurrence assessment of earth fissure based on genetic algorithms and artificial neural networks in Su-Xi-Chang land subsidence area, China

Wenjing Zhang,Li Gao,Xun Jiao,Jun Yu,Xiaosi Su,Shanghai Du 한국지질과학협의회 2014 Geosciences Journal Vol.18 No.4

Earth fissures in Su-Xi-Chang land subsidence areahave induced massive damages to the area. The non-linear characteristicassociated with the process of earth fissure formation requiresnon-linear method for evaluating the occurrence of the hazard. Based on quantification of influence factors on breeding the hazard,GA-ANN method, which integrates artificial neural networks(ANN) with genetic algorithms (GA), is developed for evaluating theoccurrence of earth fissure hazard. Six indicators, that include thedepth of bedrock burial (DBB), the degree of bedrock relief (DBR),water level (WL) (the II confined aquifer), the gradient of landsubsidence (GLS), transmissivity (T) (the II confined aquifer) andthe thickness of clay soil (TCS), are selected as the input patternsof the integrated approach, and danger index (DI) as the outputpattern. A multilayer back-propagation neural network is trainedwith 30 sets of data samples including 15 sets of earth fissure samplesand 15 sets of safety samples for defining the architecture ofANN. Subsequently, GA is employed by optimizing the initial weightsof trained ANN by minimizing the deviation of output. The efficacy ofthe integrated approach is demonstrated by comparing the deviationof output from ANN and GA-ANN for 5 testing samples andthe result shows that the GA-ANN method is more accurate thanANN in identifying the occurrence of earth fissure. The integratedmethod is applied to the assessment of earth fissure hazard in typicalregions of earth fissure. According to the classification of DI, theregions are divided into four zones ‒ danger zone, sub-danger zone,sub-safe zone and safe zone.

Sulfonylurea Herbicide Resistance Mechanism of Some Acetohydroxy Acid Synthase Mutants and New Designed Herbicides Specific to the Mutants

Mun Myong Choe,Hun Chol Kang,In Chul Kim,Hai Su Li,Ming Gen Wu,Im Shik Lee 한국잔디학회 2017 Weed & Turfgrass Science Vol.6 No.1

The mutation rate of proline in the position 197 (Pro197) in acetohydroxy acid synthase (AHAS) is highest among sulfonylurea (SU) herbicide-resistance mutants. Therefore, it is significant to investigate the resistance mechanism for the mutation and to develop the herbicides specific to the mutants. SU herbicide resistance mechanism of the mutants, 197Ser, 197Thr and 197Ala, in AHAS were targeted for designing new SU-herbicide. We did molecular dynamics (MD) simulation for understanding SU herbicide-resistance mechanisms of AHAS mutants and designed new herbicides with docking and MD evaluations. We have found that mutation to 197Ala and 197Ser enlarged the entrance of the active site, while 197Thr contracted. Map of the root mean square derivation (RMSD) and radius gyrations (Rg) revealed the domain indicating the conformations for herbicide resistant. Based on the enlarging-contracting mechanism of active site entrance, we designed new herbicides with substitution at the heterocyclic moiety of a SU herbicide for the complementary binding to the changed active site entrances of mutants, and designed new herbicides. We confirmed that our screened new herbicides bonded to both AHAS wild type and mutants with higher affinity, showing more stable binding conformation than the existing herbicides.

Sulfonylurea Herbicide Resistance Mechanism of Some Acetohydroxy Acid Synthase Mutants and New Designed Herbicides Specific to the Mutants

Choe, Mun Myong,Kang, Hun Chol,Kim, In Chul,Li, Hai Su,Wu, Ming Gen,Lee, Im Shik The Korean Society of Weed ScienceThe Turfgrass So 2017 Weed & Turfgrass Science Vol.6 No.1

The mutation rate of proline in the position 197 (Pro197) in acetohydroxy acid synthase (AHAS) is highest among sulfonylurea (SU) herbicide-resistance mutants. Therefore, it is significant to investigate the resistance mechanism for the mutation and to develop the herbicides specific to the mutants. SU herbicide resistance mechanism of the mutants, 197Ser, 197Thr and 197Ala, in AHAS were targeted for designing new SU-herbicide. We did molecular dynamics (MD) simulation for understanding SU herbicide-resistance mechanisms of AHAS mutants and designed new herbicides with docking and MD evaluations. We have found that mutation to 197Ala and 197Ser enlarged the entrance of the active site, while 197Thr contracted. Map of the root mean square derivation (RMSD) and radius gyrations (Rg) revealed the domain indicating the conformations for herbicide resistant. Based on the enlarging-contracting mechanism of active site entrance, we designed new herbicides with substitution at the heterocyclic moiety of a SU herbicide for the complementary binding to the changed active site entrances of mutants, and designed new herbicides. We confirmed that our screened new herbicides bonded to both AHAS wild type and mutants with higher affinity, showing more stable binding conformation than the existing herbicides.

있다'의 문법화에 대한 의미·화용적 연구 : 부산 방언을 중심으로

이수련 국어학회 2003 국어학 Vol.42 No.-

‘있다’는 ‘존재’라는 원형에서 ‘장소, 소유, 시간’ 따위로 그 뜻이 확장됨으로써 문법화 현상이 일어난다. 이에 따른 양상을 크게 거시적 관점과 미시적 관점으로 나누어 살피기로 한다. 거시적인 관점은 구체적인 근원영역에서 추상적인 목표영역으로 전이가 일어나는 것으로써 은유라는 기제가 관여한다. 이 때 ‘있다’는 ‘존재’에서 ‘장소, 소유, 시간’ 따위로 추상화하는 것으로 나타난다. 미시적인 관점은 환유, 비범주화와 다양화, 주관화의 기제가 관여한다. 환유는 ‘있다’가 원형인 존재에서 장소, 소유로 확대되는 공간적 인접성과 시간으로 확대되는 시간적 인접성에 관여한다. 비범주화는 ‘있다’가 부사 ‘이따(가)’로 전이될 때와 본용언이 보조용언으로 문법화 될 때 관여하는 기제로서 존재, 장소, 소유의 뜻은 고갈되고 시간개념만 남는 것에 관여한다. 주관하는 ‘-ㄹ 수 있다’가 가능성이라는 인식적인 서법으로 확대될 때와 담화표지로서 화용적 기능만 남게 될 때 관여한다. ‘가능성’이라는 서법은 간접적으로 시간개념과 관련이 있지만, 담화표지가 되면 어휘적, 문법적인 뜻은 없어지게 되고 완전히 문법화 된다. 이 글은 ‘있다’의 문법화에 관한 공시론적 연구이지만, 통시론적인 연구의 밑거름이 될 수 있기를 기대해 본다. Grammaticalization of 'itta' are considered in a wide and narrow point of two views. A wide point of views take part in grammaticalization of semantic transferring by Metaphor from concrete source domain to abstract goal domain. Abstracting of 'itta' appear 'existence>location>possession>time' schema. Narrow point of views take part in device of metonymy, decategolization, subjectification. Metonymy take part in grammaticalization in spatial contiguity transferring from archetypic existence to location, possession and in time contiguity transferring from existence to time. Decategorization take part in grammaticalization transferring of main predicate to supplementary predicate and 'itta' to adverb 'itta(ga)'. In this case, time concept remain, but concept of existence, location, possession are not exist, because of semantic bleaching. Subjectification take part in grammaticalization that '-l su itta' transfer to epistemic modality and 'itta' transfer to pragmatic function of discourse marker. I expect that synchronic researches about grammaticalization of 'itta' contribute to diachronic researches.

강선 이음형 PC 보-기둥 내부 접합부의 구조적 거동에 관한 실험적 연구

하상수,김승훈,문정호,이리형,임주혁 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

In this study, new moment-resisting precast concrete beam-column joint is proposed for moderate seismic regions. it is attempt to devise connection detail which makes workability improve and confirm effective stress transfer in the region of connection. It has the connection details which help working process simplified and construction cost reduced. as replace 9.3㎜, 7strand for reinforcement. Fore specimens for partial tension test and three specimens for beam-column interior joint test were producted. An experimental study was carried out to investigate the joint behavior subjected to reverse cyclic loading and constant axial compressive load. The test variables was transverse reinforcement in joint. Structural performance of joint is evaluated on the basis of connection strength, stiffness, energy dissipation, and drift capacity. Based on the test results, the precast connection is capable of matching or exceeding the performance of the monolithic connection and thereby provides the sufficient moment-resisting behavior to be applicable for moderate seismic regions.

대장에 발생한 샘암종에서 MUC1과 MUC2 점소 발현의 의의

이윤경,이주호,이용,심재영,박정훈,오수섭,박진실,기근홍 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.2

Background : Mucins possess the unique function of protecting and lubricating the epithelial surface and other important functions such as call growth, direct implication in the fetal development, the epithelial renewal and differentiation, the epithelial integrity, carcinogenesis, immune regulation, cellular adhesion and metastasis. Purpose : This study was done to provide the significance of alteration of MUC1 and MUC2 expression in colorectal adenocarcinoma, A series of 131 colorectal adenocarcinomas including 11 mucinous carcinomas were screened immunohistochemically for their expression of MUCI and MUC2, Materials and mehtods : Of 131 carcinomas, 76 (58,5%) were MUCI positive and 91 (68, 9%) were MUC2 positive, In normal colonic goblet cells, MUCl was not expressed but MUC2 was expressed in cytoplasm, Conclusion There were up-regulation of MUCI and down-regulation of MUC2 in colorectal carcinomas, The frequency of MUC2 positivity according to differentiation was statistically reliable. (p=0.0001)

Cloning and Characterization of a Na+/H+ Antiporter Gene of the Moderately Halophilic Bacterium Halobacillus aidingensis AD-6T

Ya Jie Zou,Li Fu Yang,Lei Wang,Su Sheng Yang 한국미생물학회 2008 The journal of microbiology Vol.46 No.4

A gene encoding a Na+/H+ antiporter was obtained from the genome of Halobacillus aidingensis AD-6T, which was sequenced and designated as nhaH. The deduced amino acid sequence of the gene was 91% identical to the NhaH of H. dabanensis, and shared 54% identity with the NhaG of Bacillus subtilis. The cloned gene enable the Escherichia coli KNabc cell, which lack all of the major Na+/H+ antiporters, to grow in medium containing 0.2 M NaCl or 10 mM LiCl. The nhaH gene was predicted to encode a 43.5 kDa protein (403 amino acid residues) with 11 putative transmembrane regions. Everted membrane vesicles prepared from E. coli KNabc cells carrying NhaH exhibited Na+/H+ as well as Li+/H+ antiporter activity, which was pH-dependent with the highest activity at pH 8.0, and no K+ /H+ antiporter activity was detected. The deletion of hydrophilic C-terminal amino acid residues showed that the short C-terminal tail was vital for Na+/H+ antiporter activity.

철근 이음형 PC 보-기둥 내부 접합부의 구조적 거동에 관한 실험적 연구

김승훈,하상수,문정호,이리형,이강철,전병갑 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

In this study, new moment-resisting precast concrete beam-column joint is proposed for moderate seismic regions. It has the connection reinforcing bars, penetrated the joint and lap-spliced with the bottom bars of precast U-beam. An experimental study was carried out to investigate the joint behavior subjected to reverse cyclic loading and constant axial compressive load. Three precast beam-column interior were tested. The variables examined were the type of connection reinforcing bars and presence of transverse reinforcement in joint. Joint performance is evaluated on the basis of connection .strength, stiffness, energy dissipation, and drift capacity. Based on the test results, the precast connection is capable of matching or exceeding the performance of the monolithic connection and thereby provides the sufficient moment-resisting behavior to be applicable for moderate seismic regions.

정맥주입 전문간호사가 삽입한 말초삽입형 중심정맥관(PICC) 사용 결과에 대한 후향적 분석

박정윤,박광옥,백미경,김세라,권혜리,양수진 대한기초간호자연과학회 2004 Journal of korean biological nursing science Vol.6 No.1

Background : Intravenous(Ⅳ) access is becoming an increasingly important part of health care today. The current drive for clinical effectiveness and cost-effective health care serves to increase the need for reliable vascular access. Venous access devices were developed to overcome problems associated with limited peripheral access and frequent venipuncture in patients with long-term therapy. Although the peripherally inserted central catheter(PICC) have become popular during recent years in USA, its procedure is rare in Korea. Purpose : The goal of this study was to analyze the PICC inserted patient data by Ⅳ CNS intervention. Method : A Total of 62 PICCs were inserted into 51 patients by the Ⅳ CNS during a 10-month period form November, 14, 2002, to October 2, 2W2. Data was obtained retrospectively through chart review. Result : The patient population included 34(54.8%) men and 28(45.2%) women, with a mean age 50.6 years. The main indication for PICC placement was to access vein in poor peripheral venous status(40.3%). The mean served interval for PICC insertions was 16.7 days(range, 2~61 days). The reasons for removal were completed therapy in 18 cases(29.0%), patient death in 13 cases(21.0%), and mechanical or functional PICC problem in 10cases(16.1%). The three PICCs removed for presumed infection, and one had only positive tip cultures(0.2%). Conclusion : PICCs are rapidly growing popularity and required an extended course of Ⅳ therapy.