The deficiency of ABCG1 and ABCG4 transporters by rare earth oxide nanoparticles induces the pulmonary alveolar proteinosis

Soyeon Jeon,Gyuri Kim,Sinuk Lee,Wan-Seob Cho 한국실험동물학회 2022 한국실험동물학회 학술발표대회 논문집 Vol.2022 No.7

Effects of red ginseng extract on the pharmacokinetics and disposition of warfarin via intestinal Cyp2c11 modulation in rats

Jeon Soyeon,Park Jin-Hyang,Kwon Mi Jeong,Choi Min-Koo,Song Im-Sook 한국약제학회 2024 Journal of Pharmaceutical Investigation Vol.54 No.1

Purpose This study aimed to develop and validate the analytical method for warfarin and its major metabolite 7-hydroxy warfarin using liquid chromatography–triple quadrupole mass spectrometry (LC–MS/MS) as well as to conduct pharmacokinetic and elimination studies on warfarin in rats. Methods The LC–MS/MS analytical method for warfarin and 7-hydroxy warfarin was used and validated. Mice were administered warfarin (1 mg/kg) with or without the coadministration of Korean red ginseng extract (RGE, 2 g/kg/day) once or multiple times for 7 days. Thereafter, the pharmacokinetics and disposition of warfarin and 7-hydroxy warfarin were measured. The underlying mechanisms of their pharmacokinetic alterations were observed by measuring Cyp2c11 expression in the intestine and liver and by investigating the inhibitory effects of RGE and ginsenoside Rb1 on warfarin metabolism in rat intestinal and liver microsomes. Results We demonstrated good linearity in a range of 20–15,000 ng/mL for warfarin and 1–500 ng/mL for 7-hydroxy warfarin by using liquid-liquid extraction with ethyl acetate. The analytical method for this study is more reliable for conducting pharmacokinetic and elimination studies with acceptable accuracy, precision, recovery, and matrix effect. During the RGE treatment, warfarin concentration increased and the metabolic activity from warfarin to 7-hydroxy warfarin decreased in rats. Moreover, RGE treatment decreased the protein expression of Cyp2c11, the responsible metabolizing enzyme of warfarin, in rat intestine but not the liver. Ginsenoside Rb1, the most abundant ginsenoside in RGE, also inhibited the metabolic activity of Cyp2c11 in the intestine but not in the liver. Conclusion The herb–drug interactions between RGE and warfarin occurred via intestinal Cyp2c11 inhibition (for single administration of RGE) as well as decreased intestinal Cyp2c11 expression (for repeated administration of RGE) in rats.

Glyphosate influences cell proliferation in cultured human kidney cells

Soyeon Jeon,Sang-Hyeop Lee,Yeon-Hui Kim,Jaechul Roh,Hyeseung Choe,Jang-Eok Kim,Heeyoun Bunch 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10

A comprehensive analysis of gorilla-specific LINE-1 retrotransposons

Jeon Soyeon,Kim Songmi,Oh Man Hwan,Liang Ping,Tang Wanxiangfu,한규동 한국유전학회 2021 Genes & Genomics Vol.43 No.10

Background Long interspersed element-1 (LINE-1 or L1) is the most abundant retrotransposons in the primate genome. They have approximately 520,000 copies and make up ~17% of the primate genome. Full-length L1s can mobilize to a new genomic location using their enzymatic machinery. Gorilla is the second closest species to humans after the chimpanzee, and human-gorilla split 7–12 million years ago. The gorilla genome provides an opportunity to explore primate origins and evolution. Objective L1s have contributed to genome diversity and variations during primate evolution. This study aimed to identify gorilla-specifc L1s using a more recent version of the gorilla reference genome (Mar. 2016 GSMRT3/gorGor5). Methods We collected gorilla-specifc L1 candidates through computational analysis and manual inspection. L1Xplorer was used to identify whether full-length gorilla-specifc L1s were intact. In addition, to determine the level of sequence conservation between intact fulllength gorilla-specifc L1s, two ORFs of intact L1s were aligned with the L1PA2 consensus sequence. Results 2002 gorilla-specifc L1 candidates were identifed through computational analysis. Among them, we manually inspected 1,883 gorilla-specifc L1s, among which most of them belong to the L1PA2 subfamily and 12 were intact L1s that could infuence genomic variations in the gorilla genome. Interestingly, the 12 intact full-length gorilla-specifc L1s have 14 highly conserved nonsynonymous mutations, including 6 mutations and 8 mutations in ORF1 and ORF2, respectively. In comparison to the intact full-length chimpanzee-specifc L1s and human-specifc hot-L1s, two of these in ORF1 (L256F and E293G) were shown as gorilla-specifc nonsynonymous mutations. Conclusion The gorilla-specifc L1s may have had signifcantly afected the gorilla genome to compose a genome diferent form that of other primates during primate evolution.

Synthesis of Cyclopentadithiophene-Diketopyrrolopyrrole Donor-Acceptor Copolymers for High-Performance Nonvolatile Floating-Gate Memory Transistors with Long Retention Time

Jeon, Soyeon,Sun, Cheng,Yu, Seong Hoon,Kwon, Soon-Ki,Chung, Dae Sung,Jeong, Yong Jin,Kim, Yun-Hi American Chemical Society 2020 ACS APPLIED MATERIALS & INTERFACES Vol.12 No.2

<P>Organic flash memories that employ solution-processed polymer semiconductors preferentially require internal stability of their active channel layers. In this paper, a series of new donor-acceptor copolymers based on cyclopentadithiophene (CDT) and diketopyrrolopyrrole (DPP) are synthesized to obtain high performance and operational stability of nonvolatile floating-gate memory transistors with various additional donor units including thiophene, thiophene-vinylene-thiophene (CDT-DPP-TVT), selenophene, and selenophene-vinylene-selenophene. Detailed analyses on the photophysical, two-dimensional grazing incident X-ray diffraction, and bias stress stability are discussed, which reveal that the CDT-DPP-TVT exhibits excellent bias stress stability over 10<SUP>5</SUP> s. To utilize the robust nature of CDT-DPP-TVT, floating-gate transistors are fabricated by embedding Au nanoparticles between Cytop layers as a charge storage site. The resulting memory devices reveal bistable current states with high on/off current ratio larger than 10<SUP>4</SUP> and each state can be distinguished for more than 1 year, indicating a long retention time. Moreover, repetitive writing-reading-erasing-reading test clearly supports the reproducible memory operation with reversible and reliable electrical responses. All these results suggest that the internal stability of CDT-DPP-TVT makes this copolymer a promising material for application in reliable organic flash memory.</P> [FIG OMISSION]</BR>

Situational Awareness를 통한 소비자 개별 맞춤형 광고 시스템 모델 연구

전소연(SoYeon Jeon),윤용익(Yong-Ik Yoon) 한국정보과학회 2014 한국정보과학회 학술발표논문집 Vol.2014 No.6

자아존중감, 부모애착, 사회적 지지가 대학생의 진로성숙도에 미치는 영향

전소연 ( Soyeon Jeon ),주은지 ( Eunjee Joo ) 아시아문화학술원 2018 인문사회 21 Vol.9 No.5

대학생들이 직업 선택에 대해 느끼는 어려움을 극복하고 사회에 잘 적응하도록 돕기 위해 진로와 관련한 연구들이 요구된다. 본 연구의 목적은 대학생의 자아존중감, 부모애착, 사회적 지지가 진로성숙도에 미치는 영향을 살펴보는 것이다. 이를 위하여 충청남도에 소재하는 대학교의 학생 총343명의 설문자료를 분석에 이용하였다. 자료 분석은 기술통계, 피어슨 상관관계, T-검증, F-검증, 그리고 다중회귀분석을 사용하였다. 연구결과는 다음과 같다. 첫째, 인구통계학적 변인(성별, 학년)에 따라 자아존중감, 부모애착, 사회적 지지와 진로성숙도에 차이가 있었다. 둘째, 자아존중감과 사회적 지지는 진로성숙도와 그리고 부모애착은 사회적 지지와 유의한 상관관계가 있었다. 마지막으로, 자아존중감과 사회적 지지는 진로성숙도에 영향을 미친 반면, 부모애착은 진로성숙도에 영향을 미치지 않았다. 본 연구결과를 바탕으로 대학생의 진로성숙도를 향상시킬 수 있는 프로그램과 상담적 개입방안 그리고 미래연구를 위한 제언을 담았다. Studies regarding career for university students is needed for them to overcome the difficulties of choosing the job and to help adjust well in this society. The purpose of this study was to identify the influences of the self-esteem, parent attachment and social support on the career maturity of university students. Subjects of the study were 343 students in university, located in Chuncheongnam-do. The data were analyzed by using descriptive statistics analysis, Pearson correlation analysis, t-test, F-test, and multiple regression analysis. The results of the study are as following. First, the result of the differences in self-esteem, parent attachment, social support, and the career maturity of university students by the demographic variable(gender, grades) were found. There were a significant correlation between four variables. Third, self-esteem and social support effects on career maturity, but not parent attachment. This study provided a data base for developing variety programs related on self-esteem, social support to university students. Implications, suggestions for future research were discussed.

지능형 소셜 로봇의 성능평가를 위한 테스트 벤치 개발

전하늘(Haneul Jeon),김소연(Soyeon Kim),전동수(Dongsu Jeon),정형진(Hyungjin Jeong),이동훈(Donghun Lee) 대한기계학회 2022 大韓機械學會論文集A Vol.46 No.2

본 논문은 multimodal human-robot interactions를 위해 개발된 지능형 소셜 로봇들의 성능평가를 위한 테스트 벤치 개발에 대하여 기술한다. 로봇과의 자연스러운 physical and emotional interactions에 대한 소비자들의 성능 기대치가 점차 높아지고 있기 때문에, 지능형 로봇은 그 세대가 거듭될수록 요소 인지 및 판단 기능들이 고도화뿐만 아니라 서로 복잡하게 통합되고 있다. 하지만 우리는 이러한 지능형 로봇들의 인지 및 판단을 위한 인공지능기술에 쏟는 노력 대비 지능형 로봇의 성능들을 종합적으로 평가하기 위한 방법 및 기술 분야에는 상대적으로 많은 성과가 없었던 것에 주목할 필요가 있다. 따라서 본 연구에서는 소셜 로봇의 HRI 성능평가를 위한 테스트 벤치 개념을 제안하고, 그 핵심 구성 요소로서 평가지표 및 평가시나리오의 개발에 주목하고자 한다. 테스트 벤치는 최대 2인의 피험자와 통합 HRI 기능을 탑재한 로봇 원형 Cube의 위치 및 자세를 실시간으로 측정할 수 있는 모션 캡쳐 시스템 및 운영/결과 분석을 위한 운영 콘솔로 구성된다. 피험자 인식/재인식, 피험자 및 소리 종류와 위치 추정과 관련된 5가지 성능지표를 제시하고 최종적으로 Cube의 인식 및 판단 견실성을 가늠할 수 있다. 결론 부분에서 시나리오별 성능의 종합적 평가 결과 및 소셜 로봇의 성능 개선 방향을 제시한다. This study describes the development of a test bench for the performance evaluation of intelligent social robots developed for multimodal human-robot interactions (HRI). As consumers" performance expectations for natural physical and emotional interactions with robots are gradually increasing, the generation of intelligent robots continues. Currently, the cognitive and judgment functions are not only advanced, but also complexly integrated with each other. However, there has been relatively little achievement in the field of methods and technologies for comprehensively evaluating the performance of intelligent robots compared to the efforts devoted to artificial intelligence technology for cognition and judgment of these intelligent robots. Therefore, the concept of a test bench for evaluating the HRI performance of social robots is proposed in this study with a focus on the development of evaluation indicators and evaluation scenarios as the key components. The test bench consists of a motion capture system that can measure the position and posture of a robot Cube equipped with integrated HRI functions with up to two subjects in real time, and an operating console for operation/result analysis. Five performance indicators related to subject recognition/re-recognition, subject and sound type and position estimation are presented, and finally the reliability of the Cube recognition and judgment can be evaluated. In conclusion, the comprehensive evaluation results of the performance for each scenario and the direction of improvement of social robot performance are presented.

오메가-3 지방산에 의한 COX-2/MMPs/VEGF 억제에 따른 대장암세포의 종양 형성 및 침윤 억제

신소연(Soyeon Shin),김용조(Yong-Jo Kim),한승현(Seung-Hyeon Han),프라산타(Prashanta Silwal),허준영(Jun-Young Heo),전영주(Young-Joo Jeon),박승길(Seung-Kiel Park),권기량(Gi-Ryang Kweon),박종일(Jong-Il Park),임규(Kyu Lim) 한국생명과학회 2017 생명과학회지 Vol.27 No.9

대장암은 미국 등 서양 국가뿐만 아니라 국내에서도 2번째로 많이 발병이 되는 암으로 알려져 있다. 역학조사에 의하면 오메가-3를 많이 섭취한 인종에서 대장암 발생빈도가 감소하고 최근 오메가-3는 수종의 암에 대해 항암작용을 나타낸다고 한다. 이에 본 연구에서는 대장암에서 DHA의 항침윤, 항혈관 신생 및 항종양 형성능 억제의 기전을 규명하여 다음과 같은 결과를 얻었다. DHA는 인체 대장암 세포주 HT29 의 증식을 농도 의존적으로 억제하였으나 AA는 거의 영향이 없었다. FACS 분석에서 DHA 처리했을 때 Sub G1 phase의 세포가 DHA의 농도 의존적으로 증가 하였다. DHA 처리 후 cleaved PARP가 증가하고, uncelaved caspase-3가 감소 하였다. HT29 세포의 침윤능은 DHA 처리에 의해 억제 되었다. DHA 처리 후 MMP-9 및 MMP-2 mRNA양이 감소 되었을 뿐만 아니라 그 promoter의 reporter 활성도 억제하였으며 VEGF promoter 활성도 DHA에 의해 억제 되었다. NF-kB promoter 활성 및 핵으로의 이동도 DHA에 의해 억제 되었다. In vivo 동물실험에서 생쥐 대장암 세포주인 MCA38에 대한 Fat-1 transgenic mice에서의 종양 형성능은 현저히 억제 되었다. 면역형광염색법을 이용한 Fat-1 transgenic mice의 종양 조직에서의 TUNEL 양성세포는 wild type mice에 비해 현저히 증가하였으나 CD31의 형광강도는 감소 되었다. 이상의 결과로 오메가-3는 대장암 세포에서 NF-kB 억제에 따른 COX-2, MMP-2 및 MMP-9 등 matrix matalloproteinase의 억제를 통한 침윤능의 억제, VEGF 억제를 통한 혈관신생의 억제등 복합적 기전에 의해 항암작용을 나타내리라 생각되며, 따라서 오메가-3는 대장암의 예방 및 치료에 유용하게 사용될 수 있으리라 생각된다. Epidemiology studies have reported a reduced incidence of colon cancer among populations that consume a large quantity of ω3-polyunsaturated fatty acids (ω3-PUFAs) of marine origin. Herein, we demonstrated a mechanism of anticancer action of ω3-PUFAs, showing that they suppressed invasion and tumorigenicity in colon cancer cells. Docosahexaenoic acids (DHA) inhibited the cell growth of HT29 cells. This action likely involved apoptosis, given that the DHA treatment increased the cleaved form of PARP and sub G1 cells. Moreover, the invasiveness of HT29 cells was inhibited following DHA treatment, whereas arachidonic acid (AA) had no effect. The levels of Matrix-metalloproteinase-9(MMP-9) and MMP-2 mRNA decreased after DHA pretreatment. DHA treatment inhibited MMP-9 and MMP-2 promoter activities and reduced VEGF promoter activity. DHA pretreatment also inhibited the activities of prostaglandin-2 (PGE2)-induced MMPs and the VEGF promoter. Cyclooxygenase-2(COX-2) overexpression increased the activity of MMPs and that of the Vascular endotherial growth factor (VEGF) promoter in HT29 cells, and DHA inhibited NF-kB and COX-2 promoter reporter activities. As shown by in vivo experiments, when mouse colon cancer cells (MCA38) were implanted into Fat-1 and wild-type mice, both the tumoral size and volume were dramatically inhibited in Fat-1 transgenic mice. Furthermore, TUNEL-positive cells increased in tumors from Fat-1 mice compared with wild mice. In immunohistochemistry, the intensity of CD31 in Fat-1 tumors was weaker. These findings suggest that ω3-PUFAs may inhibit tumorigenicity and angiogenesis as well as cancer cell invasion by suppression of COX-2, MMPs and VEGF via the reduction of NF-kB in colon cancer.

Effect of the muscle activity of each quadriceps femoris muscle according to walking on the wearable inertial sensor measurements

Haneul Jeon(전하늘),Donghyeon Noh(노동현),Soyeon Kim(김소연),Donghun Lee(이동훈) 대한기계학회 2020 대한기계학회 춘추학술대회 Vol.2020 No.12