Hypoxia-Responsive MicroRNA-101 Promotes Angiogenesis <i>via</i> Heme Oxygenase-1/Vascular Endothelial Growth Factor Axis by Targeting Cullin 3

Kim, Ji-Hee,Lee, Kwang-Soon,Lee, Dong-Keon,Kim, Joohwan,Kwak, Su-Nam,Ha, Kwon-Soo,Choe, Jongseon,Won, Moo-Ho,Cho, Byung-Ryul,Jeoung, Dooil,Lee, Hansoo,Kwon, Young-Guen,Kim, Young-Myeong Mary Ann Liebert 2014 Antioxidants & redox signaling Vol.21 No.18

<P>Aims: Hypoxia induces expression of various genes and microRNAs (miRs) that regulate angiogenesis and vascular function. In this study, we investigated a new functional role of new hypoxia-responsive miR-101 in angiogenesis and its underlying mechanism for regulating heme oxygenase-1 (HO-1) and vascular endothelial growth factor (VEGF) expression. Results: We found that hypoxia induced miR-101, which binds to the 3 ' untranslated region of cullin 3 (Cul3) and stabilizes nuclear factor erythroid-derived 2-related factor 2 (Nrf2) via inhibition of the proteasomal degradation pathway. miR-101 overexpression promoted Nrf2 nuclear accumulation, which was accompanied with increases in HO-1 induction, VEGF expression, and endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) production. The elevated NO-induced S-nitrosylation of Kelch-like ECH-associated protein 1 and subsequent induction of Nrf2-dependent HO-1 lead to further elevation of VEGF production via a positive feedback loop between the Nrf2/HO-1 and VEGF/eNOS axes. Moreover, miR-101 promoted angiogenic signals and angiogenesis both in vitro and in vivo, and these events were attenuated by inhibiting the biological activity of HO-1, VEGF, or eNOS. Moreover, these effects were also observed in aortic rings from HO-1(+/-) and eNOS(-/-) mice. Local overexpression of miR-101 improved therapeutic angiogenesis and perfusion recovery in the ischemic mouse hindlimb, whereas antagomiR-101 diminished regional blood flow. Innovation: Hypoxia-responsive miR-101 stimulates angiogenesis by activating the HO-1/VEGF/eNOS axis via Cul3 targeting. Thus, miR-101 is a novel angiomir. Conclusion: Our results provide new mechanistic insights into a functional role of miR-101 as a potential therapeutic target in angiogenesis and vascular remodeling. Antioxid. Redox Signal. 21, 2469-2482.</P>

우리나라 長壽者의 生活 및 意識調査에 관한 硏究 - Ⅴ. 過去의 健康疾病 및 苦痛에 대한 態度 -

최진호(Jin-Ho Choi),변재형(Jae-Hyeung Pyeun),최재수(Jae-Sue Choi),임채환(Chae-Hwan Rhim),김수현(Soo-Hyun Kim),김정한(Jeung-Han Kim),이병호(Byeung-Ho Lee),우순임(Soon-Im Woo),최선남(Sun-Nam Choi),변대석(Dae-Seok Byun),김무남(Mu-Nam Ki 한국식품영양과학회 1987 한국식품영양과학회지 Vol.16 No.4

우리나라 長壽者의 生活 및 意識調査硏究의 一環으로 長壽者의 過去의 健康, 疾病 및 苦痛에 대한 對處態度 등을 調査ㆍ比較한 結果는 다음과 같다.<br/> 1. 更年期(40~60歲)의 健康狀態가 『대단히 건강했다.』가 71.0%로 가장 많고, 그 다음이 『건강이 보통이었다』가 21.6%로서, 전체 장수자의 91.6%가 보통이상의 건강상태를 유지했다.<br/> 2. 老年期에 접어든 후 疾病이나 負傷을 당한 經驗이 『없었다』고 대답한 경우가 74.7%로 대부분을 차지한 반면 『있었다』고 대답한 경우는 22.6%에 불과했다.<br/> 3. 疾病이나 負傷이 『있었다』고 한 경우의 內譯을 比較하여 보면 『消火器疾患』이 32.6%로 가장 많고, 그 다음이 『腦率中ㆍ高血壓』 14.0%, 『心臟病』 14.0%, 『呼吸器疾患』 12.8%의 순으로 되어 있었다.<br/> 4. 苦痛에 대한 經驗을 調査하여 본 結果, 苦痛이 『없었다』가 55.7%, 『있었다』가 44.3%로 거의 비슷한 傾向이었고, 苦痛에 대한 經驗內譯을 보면 『가정생활』이 85.1%로 대부분을 차지한 반면 『사업』 등은 10.1%에 불과해서 가정생활에 많은 문제가 있었음을 알 수 있었다.<br/> 5. 苦痛에 대한 對處態度롤 比較하여 보면 빨리 『잊을려고 努力했다』가 51.2%로 가장 많았고 그 다음이 『계속 걱정을 했다』(33.3%), 『걱정을 하지 않았다』(7.1%)의 순이었다.<br/> 6. 長壽한 사람들의 初經年齡은 『16~18歲』가 38.8%로 가장 많고, 그 다음이 『19歲이후』가 25.2%로서 전체 장수자의 64.0%가 16세이상에서 初經이 나타났으며, 閉經年齡은 『41~43歲』가 36.4%로 가장 많고, 그 다음이 『44~46歲』가 28.5%로서 전체 장수자의 64.9%가 46歲이하에서 閉經現象이 나타났다. 따라서 장수한 사람들의 初經年齡은 현재보다 4~5年 늦은 반면 閉經年齡은 현재보다 4~5年 빨랐음을 알 수 있었다. The present study was designed to be link a part of the research on the investigation on daily life and consciousness of the longevous people in Korea, and to investigate the health conditions, and the attitude on disease and suffering of longevous people in the past.<br/> 1. By the health conditions of longevous people between 40 and 60 years, "very good" showed the highest figure of 71.0%, followed by "ordinary" (21.6%). Consequently it is found that 92.6% of total longevous people surveyed maintained the good health conditions above ordinary.<br/> 2. Of the experiences of disease and/or injury after 60 years of longevous people, "never" showed the highest figure of 74.7%, while "had" showed the only 22.6% of total longevous people surveyed.<br/> 3. Of the details of disease and/or injury after 60 years of longevous people, "digestive disease" showed the highest figure of 32.6 %, followed by "apoplexia cerebri & hypertensions" (14.0%) and "heart disease" (14.0%), and followed by "respiratory disease" (12.8%).<br/> 4. Of the experiences of suffering between 40 and 60 years of longevous people, "never" showed 55.7% of total longevous people surveyed, while the frequency of suffering decreased in the order of "once" (22.7%), "2 times" (15.8%) and "3 times" (4.7%). By the details of suffering between 40 and 60 years of longevous people, "family life" showed the highest figure of 85.1%. followed by "business" (10.1%).<br/> 5. By the attitude on suffering of longevous people, "tried to forget soon" showed the highest figure of 51.2%, followed by "continuous worried" (33.3%). and followed by "not worried" (7.2%).

우리나라 長壽者의 生活 및 意識調査에 관한 硏究 - Ⅴ. 過去의 健康疾病 및 苦痛에 대한 態度 -

최진호(Jin-Ho Choi),변재형(Jae-Hyeung Pyeun),임채환(Chae-Hwan Rhim),양종순(Jong-Soon Yang),김수현(Soo-Hyun Kim),김정한(Jeung-Han Kim),이병호(Byeong-Ho Lee),우순임(Soon-Im Woo),최선남(Sun-Nam Choe),변대석(Dae-Seok Byun),김무남(Mu-Nam 군산대학교 수산과학연구소 1994 군산대학교 수산과학연구소 연구논문집 Vol.1 No.-

The present study was designed to be link a part of the research on the investigation on daily life and consciousness of the longevous people in Korea, and to investigate the health conditions, and the attitude on disease and suffering of longevous people in the past. 1. By the health conditions of longevous people between 40 and 60 years, ""very good"" showed the highest figure of 71.0%, followed by ""ordinary"" (21.6%). Consequently it is found that 92.6% of total longevous people surveyed maintained the good health conditions above ordinary. 2. Of the experiences of disease and/or injury after 60 years of longevous people, ""never"" showed the highest figure of 74.7%, while ""had"" showed the only 22.6% of total longevous people surveyed. 3. Of the details of disease and/or injury after 60 years of longevous people. ""digestive disease"" showed the highest figure of 32.6%. followed by ""apoplexia cerebri & hypertensions""(14.0%) and ""heart disease""(14.0%), and followed by ""respiratory disease""(12.3%). 4. Of the experiences of suffering between 40 and 60 years of longevous people, ""never"" showed 53.7% of total longevous people surveyed, while the frequency of suffering decreased in the order of ""once""(22.7%), ""2 times""(15.8%) and ""3 times""(4.7%). By the details of suffering between 40 and 60 years of longevous people. ""family life"" showed the highest figure of 85.1%, followed by ""business""(10.1%). 5. By the attitude on suffering of longevous people, ""tried to forget soon"" showed the highest figure of 54.2%, followed by ""continuous worried""(23.3%), and followed by ""not worried""(7.2%).

자궁외임신 216례

남상욱,송재경,최종호,홍순도,이종학,허광현,박일영 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3

This study was undertaken to review the case series of 216 ectopic pregnancies admitted to the Department of Obstetrics and Gynecology, Pohang Sunrin Hospital from January, 1990 to December, 1991. The incidence of ectopic pregnancy was relatively high, being found to be 1 in 34.4 delivery cases. On the symptomatology, low abdominal pain was encountered in 96.8 percent, amenorrhea in 93.5 percent and vaginal bleeding in 16.2 percent in order. The fallopian tube was the most frequently, being found to be 98.6 percent, followed by the ovary with 0.9 percent and the cervix with 0.5 percent.

大學柔道選手들의 Body Density 推定回歸式 作成

남덕현,장은경,박순호 龍仁大學校 體育科學硏究所 1995 體育科學硏究論叢 Vol.5 No.1

The purpose of this study was to measure accurately the body fat of 37 male university Judo players based on the underwater weighing method, to calculate the body density and the body fat using Futrex, Impedance, Caliper, Skyndex and Ultra-sound and to present the prediction formula of residual volume and calculate the body density by regression equations based on the data from anthropometric measurements. 1. The body % fat of Judo players from the underwater weighing method was 72± 2.44%. 2. The residual volume was 1.3350±0.2476(1) by the oxygen rebreathing method and 1.0977±0.1238(1) by the indirect method of vital capacity. The regression equation using the vital capacity was : Residual volume(1)=0.2165+0.000245×vital capacity. 3. The regression equations of the body density calculated from the measurement of skinfold thickness were as follows: The body density by Caliper(g/ml)=1.0941-0.00088×subscapular-0.001026×triceps. The body density by Skyndex(g/ml)=1.0932-0.001137×subscapular-0.00070×triceps. The body density by Ultra-sound(g/ml)=1.1095-0.00306×triceps-0.0015×femoral. 4. The regression equations of the body density caculated from anthropometric measurements were as follow: 1) The regression equation of the body fat on skinfold thickness The body density(g/ml)+1.1007+0.000507×grith of hip-0.00145×grith of thigh+0.00122×grith of lower thigh. 2) The regression equation of the body density based on skinfold thickness by Caliper, grith of the body, height, weight and age. 3) The regression equation of the body density based on skinfold thickness by skyndex, grith of the body, height, weight and age. The body density(g/ml)=1.0808-0.0010The regression equation of the body density based on skinfold thickness by subscapular-0.0011The regression equation of the body density based on skinfold thickness by triceps+0.0002×height-0.0016×age. 4) The regression equation of the body density based on skinfold thickness by Ultra-sound, grith of the body, height, weigth, and age. The body density(g/ml)=1.1228-0.0030×triceps-0.0017×femora+0.0004×minimum grith of lower thigh-0.0010×age.

두개골 및 두개봉합부 초기발육과정에서의 전사조절인자인 Msx2와 DIx5의 역할

송민호,박미현,남순현,김영진,류현모,김현정 大韓小兒齒科學會 2003 大韓小兒齒科學會誌 Vol.30 No.3

두개봉합부의 조기융합으로 일컬어지는 craniosynostosis는 두개봉합부에서의 골아세포의 조기분화 및 석회화의 결과로 나타나는 선천성 발육이상이다. 최근 유전학적 연구에 의하면 homeobox gene인 Msx2의 변이에 의해 Boston-type craniosynostosis가 야기되며, 또한 Dlx5 homozygote mutant mouse의 표현형에서 두개골의 골화지연을 포함한 다양한 두개안면부위의 이상을 발견하였다는 보고가 있었다. 게다가 Msx2와 Dlx5 homeodomain protein의 상호작용에 의해 성숙골아세포의 표지자인 osteocalcin의 전사를 조절할 수 있다는 사실이 알려져 있다. 이러한 일련의 결과들은 Msx2, Dlx5 및 osteocalcin 유전자들이 두개골의 골화과정과 두개봉합부의 형태발생에 중요한 역할을 담당하고 있음을 제시해주고 있다. 두개골의 성장과 두개봉합부의 형태발생시 이러한 유전자들의 기능을 알아보기위해 mouse의 태생기 (E15-E18) 동안 osteocalcin, Msx2, 및 Dlx5 유전자들의 발현양상을 조사하였다. Osteocalcin은 E15부터 두정골의 골막에서 관찰되었으며, 발생시기가 후기일수록 강한 발현양상을 나타내었다. Msx2는 시상봉합부의 미분화간엽조직과 osteogenic front에서 강하게 발현되었으며 경막과 hair follicle에서도 관찰되었다. Dlx5는 osteogenic front를 포함한 두정골의 골막에서 강하게 발현되었으나 시상봉합부의 미분화간엽조직에서는 발현되지않아, Msx2와는 발현양상의 차이를 나타내었다. 이 결과들을 종합해볼 때, Msx2와 Dlx5 유전자는 두개골과 두개봉합부의 성장발육과정에 중요한 역할을 담당하고 있으며, BMP signaling은 두 전사조절인자들을 조절하므로써 두개골의 골화과정과 두개봉합부의 형태발생 및 유지에 관여하고 있음을 제시해주고 있다. 특히 BMP signaling에 specific downstream gene인 Msx2 및 Dlx5의 발현양상의 차이는 골아세포의 분화시 이들 유전자가 각각의 독특한 기능을 가지고 있음을 시사해주고 있다. Craniosynostosis, known as a premature fusion of cranial sutures, is a developmental disorder characterized by precocious differentiation and mineralization of osteoblasts in the calvarial sutures. Recent genetic studies have demonstrated that mutation in the homeobox gene Msx2 causes Boston-type human craniosynostosis. Additionally, the phenotype of Dlx5 homozygote mutant mouse presents craniofacial abnormalities including a delayed ossification of calvarial bone. Furthemore transcription of osteocalcin, a mature osteoblast marker, is reciprocally regulated by the nomeodomain proteins Msx2 and Dlx5. These facts suggest important roles of osteocalcin, Msx2 and Dlx5 genes in the calvarial bone growth and suture morphogenesis. To elucidate the function of these molecules in the early morphogenesis of mouse cranial sutures, wer have first analyzed by in situ hybridization the expression of osteocalcin, Msx2 and Dlx5 genes in the developing parietal bone and sagittal suture of mouse calvaria during the embryonic (E15-E18) stage. Osteocalcin mRNA was found in the periosteum of parietal bones from E15, and gradually more highly expressed with aging. Msx2 mRNA was intensely expressed in the sutural mesenchyme, osteogenic fronts and midly expressed in the dura mater during the embryonic stage. Dlx5 mRNA was intensely expressed osteogenic fronts and the periostem of parietal bones. To further examine the upstream signaling molecules of transcription factor Msx2 and Dlx5, we have done in vitro experiments in E15.5 mouse calvarial explants. Interestingly, implantation of BMP2-, BMP4-soaked beads onto the osteogenic fronts after 48 hours organ culture induced etopic expressions of Msx2 and Dlx5 genes. On the other hand, overexpression of TGFβ1, GDF-6, -7, FGF-2, -4 and Shh did not induce the expression of Msx2 and Dlx5. Taken together, these data indicate that transcription factor Msx2 and Dlx5 play critical roles in the calvarial bone and suture development, and that BMP siganling is involved in the osteogenesis of calvarial bones and the maintenance of cranial sutures through these two transcriotpn factors. Furthermore, different expression patterns between Msx2 and Dlx5 suggest their specific functions in the osteoblast differentiation.

정중운동신경과 척골운동신경의 전기생리학적 연구

김종순,이현옥,안소윤,구봉오,남건우,김영직,김호봉,류재관,류재문 대한정형도수치료학회 2005 대한정형도수물리치료학회지 Vol.11 No.2

The determination of peripheral nerve conduction velocity is and important part to electrodiagnosis. Its value as neurophysiologic investigative procedure has been known for many years but normal value of median and ulnar motor nerve was poorly reported in Korea. To evaluate of median and ulnar motor nerve terminal latency, amplitude of CMAP(compound muscle action potential), conduction velocity and F-wave latency for obtain clinically useful reference value. 71 normal volunteers(age,19-65 years;142 hands) examined who has no history of peripheral neuropathy, diabetic mellitus, chronic renal failure, endocrine disorders, anti-cancer medicine, anti-tubercle medicine, alcoholism, trauma, radiculopathy. Nicolet Viking Ⅱ was use for detected terminal latency, amplitude of CMAP, conduction velocity and F-wave latency of median and ulnar motor nerve. Data analysis was performed using SPSS. Descriptive analysis was used for obtain mean and standard deviation, independent t-test was used to compare between Rt and Lt side also compare between different in genders. The results are summarized as follows: 1. Median motor nerve terminal latency was right 3.00ms, left 2.99ms and there was no significantly difference between right and left side and genders. 2. Median motor nerve amplitude of CMAP was right 17.26mV, left 17.50mV and there was no significantly differences between right and left side and genders. 3. Median motor nerve conduction velocity was right 57.89m/sec, left 58.03m/sec and there was no significantly difference between right and left side and genders. 4. Median motor nerve F-wave latency was right 25.74ms, left 25.59ms and there was significantly differences between genders. 5. Ulnar motor nerve terminal latency was right 2.38ms, left 2.45ms and there was significantly differences between right and left side. 6. Ulnar motor nerve amplitude of CMAP was right 15.99mV, left 16.02mV and there was no significantly differences between right and left side and genders. 7. Ulnar motor nerve conduction velocity was right 60.35m/sec, left 59.73/sec and there was no significantly differences between right and left side and genders. 8. Ulnar motor nerve F-wave latency was right 25.53ms, left 25.57ms and there was significantly differences between genders.

척골 지단 신경의 전기생리학적 연구

김종순,이현옥,안소윤,구봉오,남건우,김호봉,류재관,류재문 대한정형도수치료학회 2005 대한정형도수물리치료학회지 Vol.11 No.2

The ulnar nerve extends down the arm, across the elbow, and into the hand. It provides sensation to the little and ring fingers and activates many of the small muscles in the hand. The determination of peripheral nerve conduction velocity is an important part of ulnar nerve evaluation. The electrodiagnostic value as neurophysiologic investigative procedure has been known for many years but normal value of digital nerve was not reported in korea. The purpose of this investigation was to measure the digital nerve conduction velocity of ulnar nerve for obtain clinically useful reference value and compare difference in each fingers and then compare with the other countries. 71 normal Korean volunteers (age, 19-65 years; 142 hands) examined who has no history of peripheral neuropathy, diabetic mellitus, chronic renal failure, endocrinedisorders, anti-cancer medicine, anti-tubercle medicine, alcoholism, trauma, radiculopathy. Nicolet Viking Ⅱ(EMG machine) was use for detected conduction velocity and amplitude of digital nerve in ulnar nerve. Data analysis was performed using SPSS. Descriptive analysis was used for obtain mean and standard deviation and independent t-test was used to compare with ring and little finger. Conduction velocity of the right ring finger was 57.44m/sec and little finger was 55.32msec. The left ring finger was 55.55msec and little finger was 54.11msec. Amplitude of the right ring finger was 30.28µV and little finger was 48.36µV. The left ring finger was 30.67µV and little finger was 57.76µV. There were significantly difference between ring and little in amplitude (p<.05) but there were no statistically difference between conduction velocity of ring and little finger (p>.05). The amplitude of little finger are greater than ring finger. The present results revealed that electodiagnosis can easily perform in little finger for digital nerve of ulnar nerve study.

肥滿症患者의 短期禁食療法이 體重 및 血淸 gonadotropin 濃度에 미치는 影響

경난호,李順男 梨花女子大學校 韓國生活科學硏究院 1989 韓國生活科學硏究院 論叢 Vol.44 No.-

비만증은 식생활의 개선과 경제성장에 따라 그 빈도가 증가하게 되며 과체중에 비하여 여러 가지 합병증을 초래하여 생명에 위협을 주게된다. 이러한 합병증은 수명을 단축시킬수 있기 때문에 비만증의 예방 및 치료방법이 시도되고 있으며 그중 체중감소를 위하여 금식요법을 시도하기도하나 장기간의 금식요법은 환자에게 위험부담이 크므로 단기간의 금식요법이 가끔 시도되고 있다. 그러나 이방법도 여러 가지 문제를 야기하는데 저자들은 폐경전여성에서 단기간의 금식요법에 따른 변화 특히 성선자극호르몬의 변화유무를 관찰하여 생식기능에 미치는 영향을 연구하여 다음과 같은 결론을 얻었다. 1. 10일간 완전금식후 평균 1일에 0.7kg씩 급식전 체중의 9.3%가 감소되었다. 2. 혈청 LH와 FSH의 농도변화는 10일간 완전 금식후에도 통계학적으로 의의는 없었다. 이상의 결과로 폐경전여성에서 단기 금식요법이 성선기능에 별 영향을 주지 않음을 알 수 있으나 월경전, 중, 후 기간에도 시행해보아야 할 것으로 생각된다. A short term fasting was applied on 5 premenopausal obese women. The mean body weight reduction was 0.7kg/day, 9.3% of initial weight. The serum LH and FSH concentrations were not significantly altered during fasting period comparing to those of control and refeeding period. It was suggested that a short term fasting doesn't have influence on gonadal function. But for the relationship and influence on cyclic hormonal secretion, it should be considered further.

私立大學生의 成長發育 및 營養狀態에 關한 硏究 : 中學校 入試有無過程을 通한 比較

朴淳永,具燾書,朴良元,金振浩,南炳執,朴昌植,朴喆斌 慶熙大學校 1980 論文集 Vol.10 No.-

In order to ascertain any possible changes in the physical and the nutritional status of Korean high school students before and after the abolishment of college entrance examination system, an intensive survey was conducted on the physical conditions of the incoming freshmen students (9532 males and 3428 females) of Kyung Hee University from 1972 to 1980. The finding are as below. 1. Physical growth conditions In each of the average physical dimensions of body height, body weight, chest girth and sitting height, a remarkable improvement was recorded for all age groups after the matriculation was abolished. 2. Physical and nutritional indices Relative body weight showed constant values of 35.0 in male and 32.0 in female. Relative chest girth showed the normal chest girth style in all age groups of both sexes. Relative sitting height showed a constant value of 54 for both sexes. The values of vervaeck index of th nutritional status were shown to be between 86-87 in male and 83-94 in female, Pelidisi index 91 and 92-93, Rohrer index of physical status 121-125 and 130-132, and Kaup index 206-211 and 202-210, respectively.