Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis

Bae, Kee-Hwa,Yoo, Kyoung-Hwa,Lee, Mi-Hyun,Jeong, Jae-Hun,Choi, Yong-Eui,Yoon, Eui-Soo The Korean Society of Plant Biotechnology 2013 식물생명공학회지 Vol.40 No.3

Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.

논리 언어를 이용한 객체 중심 테이타베이스 시스템에 관한 연구

이기영,이용수 서울保健大學 1992 論文集 Vol.12 No.1

Object-Oriented programming has gained a tremendous popularity in the design and implementation of emerging Artifical Intelligence(AI), Computer-Aided Design and Manufacturing(CAD/CAM), Office Information System(OIS) etc. Such a situation, following from the emergrnce of the database model using Object-Oriented concepts within the fields of database system. In this thesis, we studied Object-Oriented database system using general Object-Oriented concepts and Logic Language(i. e., Prolog).

糞便檢査에 있어서 세로판 厚層塗抹法과 簡易食鹽水浮游法의 比較檢討

李駿商,張基浩,朱炅煥,金洙鎭,林漢鍾 고려대학교 의과대학 1979 고려대 의대 잡지 Vol.16 No.1

Experiments had been made to evaluate more efficient method in stool examination for various helminth ova. Authors examined 2,016 nationwidely collected stool specimens by the two methods, cellophane thick smear method and brine floatation method. The results were as foliows; 1. Of 2,019 specimens examined by cellophane thick smear method, the positive rates of following helminth ova were; Ascaris lumbricoides (fertilized) 27.3% Ascaris lumbricoides (unfertilized) 13.6%, Trichuris trichiura 39.0%, Hookworm 0.1%, Enterobius vermicularis 0.05% Hymenolepis nana 0.04%, Taenla spp. 1.3% Clonorchis sinensis 1.1% and Metagonimus yokogawai 5.7%. And, A. lumbricoides (fertilized) 27.0%, A. lumbricoides (unfertilized) 3.8%, T. trichiura 38.1%, Hookworm 2.0%, Trichostrongylus orlentalis 0.6%, E. vermicularis 0.2%, H. nana 0.8%, and M. yokogawai 0.05% by brine floatation method. 2. The causes of inferior positive rates in some helminth ova by cellophane thick smear method were shortly discussed and suggest some opinions in improvement of detection ability. 3. The positive rates of following helminth ova were superior in cellophane thick smear method to those of brine floatation method: A. lumbricoides(fertilized), Taenia spp., C. sinensis and M. yokogawai. And, the positive rates of following helminth ova were superior in brine floatation method: Hookworm T. orientalis, H. nana and E. vermicularis. 4. Cellophane thick smear method has been known to be one of the most effective methods in mass stool examination. But, this method is not so effective in detection of certain species of helminth ova. Therefore, employment of brine floatation method added to cellophane thick smear method is desirable for the better result of mass stool examination.

β-CuZn 합금의 인장 및 파괴 거동에 미치는 변형률 속도와 온도의 영향

이기안,이종수 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.12

A series of tensile tests were carried out on a B2-type b-CuZn alloy in the temperature range, room temperature to 400oC at three different strain rates (10^(-5) sec^(-1), 10^(-4) sec^(-1), 5.2x10^(-2) sec^(-1)). The stress-strain curves could be divided into three types. Type I indicates a remarkably linear sustained work hardening to fracture, type II work hardening and subsequent necking, type III yield drop behavior and stress saturation, respectively; corresponding to the insensitive yield stress region, anomalous yield stress region, and yield stress decreasing region, in the yield strength vs. temperature graph. The ultimate tensile strength basically decreased as temperature increased, only showing characterized strength hump-up region just below T_(p). The fracture mode was changed mainly from transgranular at room temperature to intergranular at high temperatures. The elongation increases with increasing temperature in region I, and then slightly decreases in region II (anomalous yield stress region). When the specimens were tensile-fractured at high temperatures (region III, above Tp), the elongation drastically increased with decreasing strain rate, for instance from 12% at 5.2x10^(-2) sec^(-1) to 87% at 10^(-5) sec^(-1). The dependence of ductility on strain rate was mainly attributed to the thermally activated <100> dislocation climb, inducing stress relief at the grain boundaries, as evidenced by microvoids at grain facets.

腎穿細胞腫(Wilms' Tumor)8例의 病理學的 檢索

李聖洙,裵奇煥 醫藥係社 1958 綜合 醫學 Vol.3 No.6

질화 분위기에서 결정화 된 Fe_(73.5)Si_(13.5)B_(9)Cu₁Nb₃ 연자성 합금의 자기적 성질

이기선,박인수,이기안,김문철,장석훈 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.11

Amorphous Fe_(73.5)Si_(13.5)B_(9)Cu₁Nb₃ alloy was crystallized in nitriding atmosphere of mixture gas of NH₃ and hydrogen. The crystallization at 823K showed the high electrical resistivity ranging from 494 to 538 mW-cm, which was higher level compared with nano-crystalline Fe_(73.5)Si_(13.5)B_(9)Cu₁Nb₃ alloy with a resistivity of about 110 mW-cm. Through the nitridation treatments, the amorphous alloy was transformed into nano-sized crystalline Fe3Si including iron nitrides such as γ' -Fe₄N phases. EPMA-concentration depth profiles confirmed an existence of nitrogen-rich region on the surface of the alloy ribbon, leading to the preferential formation of g -Fe4N phase. The formation of γ' -Fe₄N phase leaded to an increase of the resistivity and provided stable permeability and a low core loss at high frequency.

NACK 폭주 억제를 이용한 SRM 성능 개선

李演洙,金泰勳,朴惠蓮,李基鉉 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

In this paper, we deal with the Negative Acknowledgement(NACK)-Implosion problem that arises in the multicasting environment, and present a scheme to cope with this problem, the modified version of the Scalable Reliable Multicast(SRM) protocol, called SRM-K. This motivates this work in which the NACK message are generated depending on the congestion level inside the network. The occurrence of congestion is determined by estimating the round trip time (RTT) for each message. As an indication of the congestion level, we introduce a counter in each node, whose value K in dynamically adapted to the change of the network situation, namely increases when the congestion builds up and decreases otherwise. The proposed SRM-K scheme is evaluated by performing the simulation, showing that it outperforms the SRM, in particular, in the scenario when the network situation dynamically varies.

복합재를 이용한 구조물의 균열보수 대책에 관한 연구

李晟熙,韓秀贊,韓秉基,具秉俊 弘益大學校 科學技術硏究所 1997 科學技術硏究論文集 Vol.8 No.-

Recently, composite materials have widely become available to engineers. This paper briefly explains the applications of these materials and shows how these materials can be used to repair structures with damages such as crack, notch, etc. Before the fatigue test, notched and repaired specimens with thin laminate composite are prepared. Also, test apparatus is designed to perform four point fatigue test of specimens. From the experimental result, it was found that present repair technique could be used to enhance the life of damaged structures.

당밀의 첨가가 갈근탕박 사일리지의 품질과 산양의 기호성에 미치는 영향

김성복,문계봉,이봉덕,오홍록,배형철,이수기 충남대학교 농업과학연구소 2006 농업과학연구 Vol.33 No.1

Two experiments were conducted to investigate the effects of molasses supplementation to silage materials in ensiling Galgeuntang (herbal medicine) meal on the quality of silage, and its palatability in Korean native goats. In experiment Ⅰ, molasses was added at the levels of 0, 0.5, and 1.0% to Galgeuntang meal with three replicates per treatment. In experiment Ⅱ, three 1.5-yr-old Korean native goats were used to measure palatability. In experiment Ⅰ, lactic acid contents in molasses treatments were significantly(p<0.05) higher, and pH and butyric acid contents were lower than those of non-molasses treatment. In addition, molasses treatment increased total microbial cell counts in MRS medium for lactobacillus, but decreased total microbial cell counts in PDA medium for fungi. Molasses supplementation to silage materials increased in vitro dry matter disappearance. Molasses supplementation tended to increase silage intake in Korean native goat (experiment Ⅱ), but the difference was not significant(p>0.05). It is concluded that molasses supplementation to silage materials in ensiling herbal medicine meal could improve its preservability and palatability, the higher the better.

대전광역시 양축농가의 축분뇨 관리 실태 및 VOCs 농도 조사

이봉덕,이수기,오홍록,허정민,정기철,김성복 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1

This study was carried out to investigate the style of livestock house, concentration of malodorous substances of livestock feces and livestock houses in Daejeon area. Among the livestock houses investigated, as most of cow pens(94.5%) have sawdust or chaff on the bottom, there was no leakage of feces out of pen. Most pig pens adopted slury style, but some of them currently use buffering material on the bottom. It is thought that there will be no possible contamination leakage. When it comes to hen house, all the broiler house use litters on the bottom and all the layer house use scrapper. It is also thought that there will be no possible contamination leakage. 3 out of 12 deer pens used buffering material on the bottom, 10 places were maintained in a traditional method, and 7 places left possibility of contamination leakage considering whether the roof was installed or not. The contents of ammonia, amine and volatile fatty acid in fresh feces were lower compared to rotten feces, but the concentration of sulfur-containing matter - hydrogen sulfide, methylmercapthan and ethylmercapthan were higher compared to rotten feces. In the case of malodorous ingredient in livestock houses, only small amount of ammonia and hydrogen sulfide were detected in pig pen and hen house, and other ingredients were not detectable. And those who are engaged in animal husbandry reacted negatively to the use of feed additives for decreasing malodor. In conclusion, it is not worrisome that contamination can be leaked out of animal raising facilities. But if we take into consideration that the point of investigation time is wintry season, there should be more considerate attitude. And feed additives for decreasing malodor need establishing criteria in the manufacturing process.