5-FU 투여후 백서 가슴샘에서 일어나는 형태학적 및 생화학적 변화

손영탁,김선,정옥,한승로,김수일,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2

Thymus is a lymphoid organ forming T-cells from hematogenous stem cells. Apoptosis of thymocytes in the cortex of thymus is so rapid that cannot find easily with routine histological techniques. In this experiment, TUNEL immunohistochemistry and TEM were used to detect apoptotic changes after 5-fluorouracil treatment in the thymus of the rat. In addition, SDS-PAGE was carried to reveal protein changes along the apoptotic changes. The results were as follows ; 1. Thymocytes in the cortex were decreased markedly and many apoptotic cells were found in the cortex on DAT 3. 2. Relatively small-sized apoptotic bodies induced by 5-FU were detected on DAT 3 at the cortex, and clustered in partly. 3. On DAT 6, new germinal centers formed in the peripheral cortex, and many apoptotic bodies of various size and shapes were detected in the germinal center. 4. In SDS-PAGE, a protein with molecular weight of 123kDa was increased on DAT 6. According to the above results, it could be concluded that 5-FU acted directly on the thymocytes and induced apoptotic changes on DAT 3. But, on DAT 6, the period of recovery, new germinal centers were formed in the cortex, in which active B cell production to compensate the loss of thymocytes was noticed. To make new T-cells from B-cells and to eliminate unnecessary B-cells, vigorous apoptosis in the germinal centers should occur, and 123kDa proteins which considered as integrins should act as transmembrane signaling molecules in this process.

우리 나라 자유지대 제도 도입과 효율적 추진방향 : Focus on Pusan 부산지역을 중심으로

孫愛暉,李秀昊 韓國海洋大學校 人文社會科學大學 1999 韓國海洋大學校 人文社會科學論叢 Vol.- No.6

Abstract Shipping industry continues to face dramatic changes in their environment, ranging from development in containerization, computerization and telecommunications to the emergence of global marketplace. Perhaps the most significant trend - representing both a threat and opportunity - is the increasingly competitive nature of shipping service market. As the deregulation in the world shipping industry has continued. environments such as political, economic, and technological changes affecting shipping industry has made shippers-carriers relationship as major concern for container shipping company. As shown in The U.S. Ocean Shipping Reform Act of 1998, most of world container shipping companies have perceived customers satisfaction to be a source to enhance the competitive advantage in a fierce competitive situation. In order for the shipping companies to cope with those improvements and challenges. they should analyze and clarify customer's DM(decision making) process for the purchase of container shipping service. The purpose of this study was to explore the degree of relationship between the determinants of shipper's DM process and the information search activities for the container shipping service. An empirical study was carried out to examine how shippers make purchase decision by the provision of statistical evidence showing the impacts of involvement, experience and knowledge of the information search activities.

간장 및 비장손상에서 국소지혈제로써의 인체양막의 이용에 대한 실험적 연구

손수상,강중신,김인호 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

The hemostatic mechanism of action of collagen appears to involve both activation of the intrinsic clotting mechanism via factors XII and XI and a platelet interaction consisting of adherence and aggregation of platelets vis adenosine diphosphate release. Of many commercially available local hemostats, the microcrystalline collagen(Avitene) is known to be one of the most effective agents. Human amniotic membrane contains collagen that initiates platelet aggregation and activation of the intrinsic pathway of the clotting mechanism. The authors studied the hemostatic effectiveness of the human amniotic membrane and the results were compared with that of Avitene web, Collastat and Gelfoam. Canine liver and splenic bleeding models were used. Human amniotic membrane and Avitne web had similar effectiveness(97.2%) and were more effective than Collastat(77.8%) or Gelfoam(61.1%). First application success rates also revealed that amniotic membrane(80.6%) and Avitene web(77.8%) were more successful than others(47%). Human amniotic membrane is an effective topical hemostatic agent in experimental canine lever and splenic wounds when compared with several currently used agents and can also result in cost savings.

황사현상시 대기중 충부유분전(TSP)와 TSP중의 중금속 및 무기성 이온 조사 연구

손종렬,정호철,양광수,이용성 高麗大學校 倂設 保健大學 保健科學硏究所 1998 保健科學論集 Vol.24 No.1

The study was performed to investigate the influence of dust transportation from China contient to Korea by Yellow Sand phenomena and evaluate the aerosol during the period of Yellow Sand phenomena in Seoul, from Apr. to Oct. 1998. The TSP(Total Suspended Particulates) was collected by High Volume Air Sampler. The concentration of heavy metals in TSP were analysed by AAS and the inorganic ion of TSP were analysed by IC. The results were as follows: 1. The average concentration of TSP were 249.18㎍/㎥ at sampling point during the Yellow Sand phenomena were twice higher than that in Non-Yellow Sand which was 121.98㎍/㎥. 2. The average concentration of heavy metals in TSP during Yellow Sand phenomena and Non-Yellow Sand were Zn>Al>Fe>Pb>Mn>Cr>Ni>Cu>Cd. 3. In comparion with the ordinary time, the average concentration of anionic ions in TSP during Yellow Sand phenomena were Cl^(-)>S0₄^(2)>NO₃^(-)>F^(-)>P0₄>Br^(-), those of Non-Yellow Sand were Cl^(-)>NO₃^(-)>SO₄^(-2)>F^(-)>PO₄^>Br^(-), and that of cationic ions in TSP during Yellow Sand phenomena were Na^(+)>K^(+)>NH₄^(+).

일차성 식도이완불능증의 풍선확장술후 치료효과의 객관적 평가지표

손영해,나호영,조성범,주영은,김현수,최성규,유종선,김세종 全南大醫大 2001 전남의대학술지 Vol.37 No.2

안면 비대칭의 평가를 위한 기준에 관한 정보 두부 방사선 계측학적 연구

백선호,안병근,김선해,손홍범,한호진,강수만 대한치과교정학회 1993 대한치과교정학회지 Vol.23 No.1

This study was undertaken to investigate the middle having the least difference between the right and left structures among the lines that had been used in the study of the craniomaxillofacial asymmetry. The sample of this study consisted of twenty six Korean girls(average 18.9 years old) having normal facial appearance and occlusion. On the frontal cephalometric films of the sample, we divided the whole craniomaxillifacial area into four portions, i.e., cranial, upper facial, lower facial, and dental portion. So, we have found the midlines having the least difference in the whole craniomaxillofacial area itself, and in the each divided four portions, furtherly in the other portions from aimed portion. The findings were as follow : 1. In the whole craniomaxillofacial area, the connecting line between crista galli and anterior nasal spine and the perpendicular bisecting line between right and left foramen rotundrums were suitable for the midline. 2. In the cranial portion, established all six lines were suitable for midlines. In the other portions, the perpendicular bisection line between both condylion, the line passing the contact point between right and left mandibular central insisiors among the perpendicular lines between right and left mandibular central incisial tips were suitable midlines for evaluating the asymmetry of cranial portion. 3. In the upper facial portion, the perpendicular bisecting line between right and left zygions was the most suitable midline. In the other portions, the line between the crista galli and the most superior point of the odontoid process, the perpendicular bisecting line between right and left gonions, the perpendicular bisecting line between right and left condylions, and perpendicular bisecting line between right and left foramens rotundum were suitable midlines for evaluating the asymmetry of the upper facial portion. 4. In the dental portion, the perpendicular bisecting lines between right and left buccal cusps of both maxillary first molars and between right and left mandibular first molars were suitable midlines. In the other portions, the perpendicular bisecting line between right and left landmarks crossing the lesser wing of the connecting line between crista galli and prosthion were suitable midlines for evaluating the asymmetry of dental portion. 5. In the lower facial portion, the perpendicular bisecting lines between right and left condylions and between right and left gonions were suitable midlines. In the other portions, the line between the crista galli and anterior nasal spine, the perpendicular bisecting line between right and left foramen rotundums, and the perpendicular bisecting lines between right and left buccal cusps of both mandibular first molars and between right and left maxillary first molars were suitable midlines for evaluating the asymmetry of the lower facial portion.

미용사들의작업관련성 근골격계 장애에 관한 연구

박수경,최영진,문덕환,전진호,이종태,손혜숙 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.3

목적 : 미용사의 작업관련 근골격계 장애 실태와 관련 요인을 알아보고자 한 것이다. 방법 : 최종 대상이 된 미용사 267명에 대하여 2000년 3월부터 5월까지 Karasek의 J7Q와 N10SH의 표준화 설문지를 이용하여 일반적 특성, 직무관련 스트레스, 근골격계 장애 자각증상 등을 자기 기입식으로 작성하게 하였다. 결과 : N10SH 감시기준에 의한 근골격계 자각 증상 호소율은 전체적으로 94.4%으로 높은 편이었으며, 각 신체부위별로는 어깨 부위(61.0%), 목 부위(59.9%), 허리 부위(53.2%), 손 및 손목 부위(41.6%)의 증상 호소율이 비교적 높게 나타났으며, 상대적으로 무릎 및 허벅다리 부위(36.7%), 발 및 발목 부위(34.8%), 등 부위(28.8%), 팔 및 팔꿈치부위 (28.5%), 종아리 부위 (28.5%), 손가락 (22.8%) 등은 낮았다. 다변량 분석 결과, 미용사들의 WRMDS는 직무 스트레스와 건강관련 습관 등에 영향을 받은 것으로 관찰되었으며, 부위별로 목부위는 직무의 불안정성, 허리부위는 여자, 음주, 결정의 허용범위, 손 및 손목부위는 음주, 무릎 및 허벅다리 부위는 흡연, 발 및 발목부위는 흡연, 결정의 허용범위, 그리고 등부위는 직무의 불안정성 등이 유의한 요인으로 관찰되었다. 결론 : 주관적인 증상 호소만을 이용한 제한점에도 불구하고 산업보건의 명확한 대상이 되지 않고 있는 미용사 등의 서비스업 종사자에 대한 WRMDS 연구의 기초자료로서 활용될 수 있을 것이다. Objectives : To determine the prevalence and related factors of work related musculoskeletal symptoms in hairdressers. Methods : Informations on general characteristics, job strain, and musculoskeletal symptoms were obtained by a self-administered questionnaire, through adopting NIOSH instrument and JCQ (Job content questionnaire) , from 267 hairdressers, between March and May, 2000. Results : The symptom prevalence by N10SH surveillance criteria in total was high as 94.4%; shoulder (61.0%), neck (59.9%), low back (53.2%), hand and wrist (41.6%), etc. In multiple logistic regression, significant factors affecting the symptoms by body region , job insecurity on neck; gender, alcohol, decision latitude on low back; alcohol on hand and wrist; smoking on thigh and knee; smoking, decision latitude on ankle and foot; job insecurity on upper back. Conclusions : Musculoskeletal symptoms of hairdresser were highly prevalent, and associated with job strain and their health habits. This Is a basic data of work related musculoskeletal disorders among hairdressers who are not the subjects of occupational health service at the present time in Korea.

야간 혈압 변동과 좌심실 질량, 이완기 기능 및 QT 간격과의 관계

김수중,이병호,손일석,강흥선,조정휘,김권삼,송정상,배종화 대한간질학회 2003 대한간질학회지 Vol.9 No.1

DL-Ethionine 투여로 인한 간세포의 단백합성기구들의 초기변화에 관한 전자현미경적 연구

조수호,손태중 慶北大學校 醫科大學 1972 慶北醫大誌 Vol.13 No.2

흰쥐에 ethionine을 체중 gm당 0.2㎎을 단회 복강내에 주입하고 간세포의 단백합성기구에 일어나는 초기변화를 전자현미경으로 관찰하고 얻은 결과를 요약하면 다음과 같다. Ethionine을 복강내로 투여하면 투여 10분 내에 이미 membrane-bound ribosome의 탈락, polyome의 감소, monoribosome 증가 및 polysome사이의 filament의 소실등이 일어나고, 이어서 rER의 확장과 Golgi complex의 위축등이 일어났는데, 이와 같은 변화들은 시간이경과함에 따라 더욱 심하여지는 경향을 나타내었다. 이와같이 빠른 시간내에 단백합성에 관여하는 소기관에 형태학적으로 인정할 수 있는 변화가 나타나는 것으로 보아 ethionine으로 인한 간세포 상해의 기전은 ATP감소 이외에 다른 대사장애 내지는 ethionine의 직접적인 영향도 배제 할 수 없을 것으로 생각된다. It is well known that ethionine treatment induces decrease of ATP concentration and resultant inhibition of RNA and protein synthesis in the live cell. Ethionine traps adenosine forming S-adenosylethionine which results in deficiency of available adenosine for synthesis of ATP. But the relation of the morphological changes to the biochemical changes is still uncertain. In an attemp to clarify the initial changes of the cytoplasmic organells involved in protein synthesis, the author injected intraperitoneally 4 ㏄ of 1.25% ethionine to the rats of fasted and nonfasted. Animals were killed at 10,30, 60, 120 and 240 minutes after the injection and the livers were examined by light and electron microspcopes. The liver cells showed shedding of membranebound ribosomes, decrease of polysomes with loss of their filaments, and increase of monoribosome within 10 minutes after intraperitoneal injection of ethionine. Also noted were dilatation of rough surfaced endoplasmic reticulum and atrophy of Golgi complex. The changes were more marked in later time. Such changes of cytoplasmic organells, which could be recognized within such early time, suggested the possibility of derect action of ethionine to the cytoplasmic organells, besides the toxic effects through ATP depletion or other metabobic derangements.

다자간 투자협정이 우리 나라 해운기업에 미칠 영향에 관한 연구

이태우,이수호,손애휘 韓國海洋大學校 人文社會科學大學 1997 韓國海洋大學校 人文社會科學論叢 Vol.- No.5

The purpose of this study is to explore some expected impacts of the MAI(Multilatera1 Agreement on Investmentm) on Korean shipping companies and to suggest the strategies to deal with the increasingly competitive international shipping market as the MAI comes into force. The MAI is a comprehensive international investment treaty, which includes provisions for a high-level investment liberalization and protection, and an effective procedure for disputes settlement. It is expected to bring about substantial changes in competitive conditions and investment environment for Korean shipping companies. While Korean shipping companies may be helped by being able to tap on foreign funds and off-shore shipping finance, they will face a tougher competition from foreign shipping companies allowed to make investment and participate in domestic shipping markets. The Korean government is recommeneded to overhaul the present system of regulations and restrictions against the domestic shipping industry. On the one hand, larger shipping companies should make efforts to intensify their global operation, and smaller companies should also broaden their operation to nicne market and form a closer cooperative network to cope with a new market environment.