국화과 식물 추출물의 HepG2 및 HeLa 암세포에 대한 세포독성 효과

배송자,한은주,노승배 신라대학교 자연과학연구소 1999 自然科學論文集 Vol.7 No.-

Dietary composition is a major determinant of cancer risk in human and experimental animals. Major and minor components of the diet may enhance or suppress the metabolism of carcinogens. In this study, the methanol layer, methanol and hexane partition layers of compositae species including Artemisia princepa var. orientalis, Arctiem lappa, Chysanthemum coronarium, Lactucascariola and Carthamis tinctorius were screened for the cytotoxicity on human hepatocellular(HepG2) and human cervix epitheloid carcinoma (HeLa) using MTT method. The methanol partition layer of Artemisia princeps var. orientalis(APMM) showed the strongest cytotoxic effects on HepG2 and HeLa cells among the other compositae species extracts. The cytotoxicity of APMM on HepG2 and HeLa cells were 94% and 95%, respectively a dose of 50㎍/ml of extracts.

폐기되는 성게 껍질의 항균 활성 효과

배송자 신라대학교 자연과학연구소 2004 自然科學論文集 Vol.13 No.-

This study was performed to determine the antimicrobial activity of waste food fractions, Anthocidaris crassispina(AC) on several microorganisms. The AC extracts from methanol was fractionated to four different types, which were hexane(ACMH), methanol(ACMM), butanol (ACME) and water(ACMA). Among the various solvent fractions, ACMB showed stronger antimicrobial activity against Bacillus subtilis. These results suggest that the waste food fractions, AC peel may have developed into an bioactive natural antimicrobial product.

고들빼기 에타놀 추출물이 인지질막 유동성에 미치는 영향

배송자 신라대학교 자연과학연구소 1997 自然科學論文集 Vol.3 No.-

Liposomes have been widely employed as biomemtrane-mimetic system and drug delivery system. The effects of various drugs on the fluidity of liposomes were investigated. In this study, the effects of Ixeris sonchifolia H.(IS) which is used for food material on the fluidity of dipalmitoylphosphatidylcholine(DPPC) liposome were measured with differential scanning calorimetry(DSC). The thermograms of the DPPC liposomal bilayers incorporated with the Ixeris sonchifolia H. were obtained, and the enthalpy changes and the sizes of cooperative unit of the transition were calcurated. The results showed that incorporation of the Ixeris sonchifolia H. into the liposomal bilayers effectively reduced the transition temperature at which the transition from gel state to liquid-crystalline state occurs, broadened the thermogram peaks, and reduced the ratio of Van't Hoff to calorimetric enthalpies. These results indicate that the Ixeris sonchifolia H. might have significant effects on the fluidity of biological membranes.

쑥 추출성분의 암세포 증식억제와 Quinone reductase 유도 활성 효과

배송자,박윤자,김미향 가톨릭대학교 자연과학연구소 2001 自然科學論文集 Vol.9 No.-

In this study, Artemisia princeps var. orientalis(AP), the Compositae species, were extracted using methanol(APM) and the extracts was fractionated to five different types which are hexane(APMH), ethylether(APMEE), ethylacetate(APMEA), butanol(APMB) and aqueous(APMA) partition layers. The various partition layers were screened far the cytotoxic effects on HepG2, MCF-7 and C6 cells by MTT assay and for their ability induce quinone reductase(QR) in HepG2 cells. Compared with the other partition layers of AP, the APMEE showed the strongest cytotoxic effects on HepG2, MCF-7 and C6 cells. The quinone reductase(QR) induced activity in HepG2 cells, grown in the presence of APMEE and APMH, were 5.08 and 2.99 times more effective compared with the control value of 1.0 respectively. Therefore, based on these results, the ethylether partition layer(APMEE) of the Artemisia princeps var. orientalis may have that potentially useful cancer chemoprevention effect on the human cancer cells, HepG2, MCF-7 and C6. Key word : Artemisia princeps var. orientalis, MTT assay, quinone reductase(QR)

Silla University Publication

배송자 新羅大學校 1982 論文集 Vol.13 No.-

1. 가시부에서, Vitamin K₃와 Dihydroxy benzene 사이의 Charge-transfer Complex peak 흡수대가 나타났으므로, 전자수용체인 Vitamin K₃ 와 전자공여체인 Dihydroxy benzene 사이에 전하이동착체 형성을 확인할 수 있었다. 2. Benesi-Hildebrand식에 의하여 Vitamin K₃와 Hydroquinone과의 Charge-Transfer Complex 형성 결합 상수는 0.500-0.351 mole ??, 그 몰 흡광율 ∈는 628ℓ mole-1 cm-1이었다. Vitamin K₃와 Resorcinol과의 Charge-Transfer Complex 형성 결합 상수는 0.388-0.322 mole ℓ-1, 그몰흡광율은 486 ℓ mole-1 cm-1 이었다. 3. Hydroquinone의 경우 ??는 540 cal ?? , ??는 -1935 cal ??, ??는 -8 eu 이었으며 Resorcinol의 경우 는 618 cal , 는 -1150 cal mole-1 , 는 -6 eu로서 이들 사이의 착체형성 반응은 엔탈피 감소에 의하여 이루어짐을 알 수 있었다. 4. 이 결과에서 Vitamin K₃와 Dihydroxy benzene과의 전하이동착체 형성능력은 Hydroquinone이 Resorcinol의 경우보다 더 안정한 착화물을 형성하였다. 이는 Hydroquinone의 P-위치에 있는 두 개의 수산기의 전자공여 능력이 보강되어 Benzene 핵에 전자밀도가 더 커지고 또한 Hydroquinone 분자의 대칭성 때문에 더 큰 결합력을 나타내는 것으로 생각된다. The Charge-Transfer Complex formations between 2-methyl-1,4-naphthoquinone, Vitamin K₃ and Dihydroxy benzenes such as Hydroquinone and Resorcinol were ascertained by observation of Charge-transfer absorption in the visible spectral region. The association constants and molar absorptivities of these complexes at different temperatures were determined by the Benesi-Hildebrand Plots. The enthalpy changes were measured by the Van't Hoff plots, and the free energy and entropy changes of the reactions were calcurated by the conventional thermodynamic relations. These results showed that Hydroquinone has higher tendency for the Charge-transfer Complex formation with Vitamin K3 than Resorcinol. This higher affinity of Hydroquinone than Resorcinol was ascribed to the higher election density due to the potentiation of the election-donating ability of P- dihydroxy groups in the Hydroquinone, and better fitness to the acceptor molecule due to the symmetry of the Hydroquinone molecule.

당귀 추출물이 liposome 의 안정성 및 유동성에 미치는 영향

배송자,김경옥,노승배 신라대학교 자연과학연구소 1997 自然科學論文集 Vol.4 No.-

인지질 liposome의 안정성과 유동성에 대한 당귀 추출물의 영향을 알아보기 위해 여러 종류의 인지질을 만들어 실험한 결과 PC계 인지질 중 DPPC를 사용하여 인지질 liposome을 만들고, 막안 정성에 대한 영향을 calcein을 봉입한 liposome에 fluorescence self-quenching방법으로 측정하였으며, 막유동성에 대한 영향은 fluorescence polarization방법으로 P값을 측정하였고 그 결과를 다음과 같이 요약한다. 1. Phosphatidylcholine(PC)계 인지질 중 acyl chain의 C수가 14개인 dimyristoyl phosphatidylcholine(DMPC)과 C수가 16개인 dipalmitoyl phosphatidylcholine (DPPC) 및 여러 인지질의 혼합물인 egg phospatidylcholine(egg PC)을 사용하여 liposome 형성의 안정화와 막안정화에 대한 비교 실험을 하였다. 이중 liposome의 안정성이 가장 좋은 것은 egg PC였고, DPPC 그리고 DMPC의 순으로 calcein의 leakage %가 저하하였다. 2. 막 안정성에 미치는 당귀 추출물의 영향 : DPPC liposome에 당귀의 ethyl alcohol과 hexane 추출물을 가한 결과, DPPC만으로 만든 liposome보다 calcein leakage%가 감소하였으며, 추출용매에 다른 막안정화에 미치는 영향은 거의 유사하였다. 이와 같은 경향은 또한 인지질 liposome에 다른 약물을 첨가했을 경우 calcein의 leakage%정도보다 훨씬 그 영향이 탁월함을 보여주었다. 즉 당귀 추출물의 소수성 부분은 인지질 liposome의 상전이온도 이하(Tm. 41∼42℃)인 25℃에서 지질 이중 층의 rigid한 부분의 공간 속으로 깊숙히 들어가 막안정성 증가에 의한 인지질막의 condensing effect에 현저한 영향을 미침을 알 수 있었다. 3. 막 유동성에 미치는 당귀 추출물의 영향 : DPPC로 만든 liposome에 DPH로 labeling한 후 온도 증가에 따른 DPH의 fluorescence polarization값(P)의 측정에서 DPPC만으로 만든 liposome의 경우 상전이온도 이전의 gel 상태에서 상전이온도(phase transition temperature, Tm)로 되면서 P값이 현저히 저하하였으며, 당귀의 ethyl alcohol과 hexane추출물을 농도 별로 첨가했을 때는 35℃에서 시작하여 상전이온도 이전까지 현저히 P값의 저하가 일어남으로서 인지질 막의 유동성 증가를 알 수 있었으며 상전이온도 근처에서는 P값의 저하 경사가 완만해져서 온도의 증가에 따른 P값의 변화는 미미하였으나 상전이온도 이후에서는 DPPC liposome의 P값보다 당귀 추출물 첨가의 경우가 상대적으로 그 정도가 훨씬 증가된 상태였다. 이 결과에서 상전이온도 이전에서의 당귀 추출물의 막유동성 증가 효과를 읽을 수 있었고, 상전이를 전후해서 증가된 유동성은 상전이온도 이후에서는 온도 증가에 따라 미미하였으며, 오히려 상전이온도 이후에서는 DPPC만의 liposome보다 상대적으로 추출물의 농도 증가에 따른 P값의 변화가 미미하여 막유동성의 감소를 볼 수 있었다. 이상의 결과에서 당귀의 ethyl alcohol과 hexane 추출물의 소수성 부분은 DPPC의 상전이온도 이전에서는 인지질 liposome의 이중층으로 깊숙히 들어가서 막안정성과 막유동성을 증가 시키므로써 옛부터 보혈강장 및 정혈제로서 민간약제로 쓰이던 당귀 추출물이 인지질 세포막에 대한 생리적 활성을 일으킬 수 있는 촉진제로서의 가능성을 시사해 주었다고 볼 수 있다. We investigated the effects of the Angelica Radix extract on the physicochemical properties of biological membranes such as membrane stability and fluidity employing the phospholipid liposomal membranes as a biomembrane-mimetic system. The leakage of encapsulated calcein from the liposome and the fluorescence polarization of DPH labelled in the liposomal membrane were employed as the parameters of the stability and fluidity of the liposomal membrance. The results of the experiments showed that the addition of the Angelica Radix extract to the phospholipid exerted some effects stabilizing the barrier function of the membrane and increasing the fluidity of the membrane in proportion to the concentration of additive. These results suggest that the activities of the Angelica Radix extract to enhance stability and fluidity of the liposomal membrane might have implication in their biological activities.

扶正益氣方의 抗癌活性과 免疫調節作用에 관한 硏究

具滋權,宋昊哲,金東熙,徐榮培,朴陽春,金聖勳 대한본초학회 2000 大韓本草學會誌 Vol.15 No.1

To evaluate the antitum or activity and immune response Bujungikgibang(BJ1GB) studies were done experimentally. The results were obtained as follows: 1. BJIGB extracts exhibited a weak cytotoxicity against SK-OV-3, and SK-MEL-2 cell lines. While it showed cytotoxicity against B16-Fl0 cell line up to 30% of control at concentration of 0.5 mg/ml. 2. BJIGB extracts significantly showed inhibitoty effect on DNA topoisomerase 1 from calf thymus in a dose-dependant manner. 3. BJIGB extracts showed antiadhesive rates against A549, at 30% at 0.5 mg/ml, 60% at 1 mg/ml 4. BJIGB extracts inhibited CAM angiogenesis up to 60%. 5. BJIGB extracts prolonged life span of ICR mice bearing S-180 sarcoma cells up to 130.5%. 6. In FACS analysis, the population of helper T cell was increased weakly, while cytotoxity T cell was decreased insignificantly. 7. The production of nitric oxide in RAW cell stimulated by LPS was incresed at 0.5mg/ml or more in dose dependant fashion as compared with control. 8. Proliferation rate of splenocyte was increased at 0.5mg/ml or more a dose dependant fashion as compared with control. These results suggested that BJIGB extracts might be usefully applied for treatement of cancer and also it was nesessary to do more studies about its mechnisms.

양파껍질 분획물의 암세포 증식억제 효과

전광혜,배송자 신라대학교 자연과학연구소 2003 自然科學論文集 Vol.12 No.-

This study was performed to determine the anticarcinogenic activities of the Onion Peel(OP) on HepG2, MCF-7, C6 and HeLa cells. The OP extracts from methanol(OPM) were fractionated to five different types, which were hexane, ethylether, ethylacetate, butanol and water. The experiment was conducted to determine cytotoxicity of five different partition layers of methanol extract of OP on HepG2 MCF-7, C6 and HeLa cells by MTT assay. The fraction of OPM, OPMEE and OPMB were showed the strong cytotoxic effects on HepG2, MCF-7 and C6 cell lines. The ethylether fraction of OPM (OPMEE) was shown the strongest cytotoxic effects on the other fractions of OPM. These results suggest that the waste food fractions, OP may have developed into an bioactive health product.

막걸리 농축액의 투여가 흰쥐의 혈중 지질 함량에 미치는 영향

김미향,김원희,배송자 가톨릭대학교 자연과학연구소 2001 自然科學論文集 Vol.9 No.-

동동주, 청맥 농축물의 투여가 흰쥐의 성장률, 혈청 효소활성 혈청 지질에 미치는 영향을 조사하였다. 실험동물의 체중 증가량은 동동주, 청맥 농축물의 농도별 투여가 대조군과 비슷한 경향을 나타내었으므로, 동동주, 청맥의 투여가 성장률에 지장을 주기 않음을 알 수 있었다. 총 콜레스테롤 함량, 중성지질 함향은 DD1군과 CM1군에서 대조군 보다 낮은 경향을 나타냈고, LDL함량은 DD1군에서 대조군 보다 낮은 경향을 나타냈다. 이러한 결과로부터 동동주 8g/kg 투여는 25%(v/v) 에탄올 5g/kg/day 경구 투여가 만성중독을 일으킬 수 있는데 반해 혈중 지질 함량을 감소시키므로 혈중 중성지질 농도 증가로 인한 심질환 예방에 기여 할 것으로 사료된다. Makkoli is a traditional and general alcoholic beverage in Korea. It's brewed from rice, shows an alcohol concentration of about 16%, has a characteristic fragrance, is slightly acidic, and has a sweet taste. Recently, taking these tradition alcoholic beverages are decreased. We examined the effect of Dongdongju and Cheongmaek kinds of Makkoli on serum enzyme activity and lipid concentration in rats. Sparague-Dawley rats were randomly assigned to groups as follows: control group, Dongdongju 8g/kg administered group(DD1), Dongdongju 1.6gkg administered group(DD2), Dongdongju 0.8g/kg administered group(DD3) and Cheongmaek 8g/kg administered group(CM1), Cheongmaek 1.6g/kg administered group(CM2), Cheongmaek 0.8g/kg administered group(CM3). Experimental diets were fed for 6 weeks. The body weight gain, seram enzyme activity and serum lipid values of all groups were compared with control group. There were no differences in growth. Alkaline phosphatase and GPT activities of DD3 and CM2 were lower than control group, but no significant differences. The total cholesterol concentrations of DD1 and CM1 were lower than that of control group. The triglyceride concentration of DD1 and CM1 was the same as results of total cholesterol concentration. These results suggest that Dongdongju and Cheongmaek positively influenced on serum lipid concentration.

도라지가 난소를 절제한 흰쥐의 혈 중 지질 함량 변화에 미치는 영향

김미향,김원희,박미라,한희선,배송자 신라대학교 자연과학연구소 2003 自然科學論文集 Vol.11 No.-

This study was designed to observe the effects of the feeding Platycodon grandiflorum A.DC(PG) extract on the improvement of the lipids on serum in ovariectomized rats. To investigate the effects of ethanol extract, Sprague-Dawley female rats were randomly assigned to groups as follows: Sham, OVX-control and ovariectomized rats supplemented with PG at 50㎎/㎏ bw/day. Body weight gain was not significantly different in groups. The levels of total cholestcrol was higher in ovaiectornized control rats than Sham-operated rats, but supplementation of PG ethanol extracts at 50mg/kg bw/day decreased the level of cholesterol in surum. These results might be expected that ethanol extract of PG is believed to be possible protective effects for the fatty serum increasing serum lipid.