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- 저자 : Shang Chu (검색결과 5 건)
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Shang, Barry Z.,Chang, Rakwoo,Chu, Jhih-Wei American Society for Biochemistry and Molecular Bi 2013 The Journal of biological chemistry Vol.288 No.40
<P>Interprotein and enzyme-substrate couplings in interfacial biocatalysis induce spatial correlations beyond the capabilities of classical mass-action principles in modeling reaction kinetics. To understand the impact of spatial constraints on enzyme kinetics, we developed a computational scheme to simulate the reaction network of enzymes with the structures of individual proteins and substrate molecules explicitly resolved in the three-dimensional space. This methodology was applied to elucidate the rate-limiting mechanisms of crystalline cellulose decomposition by cellobiohydrolases. We illustrate that the primary bottlenecks are slow complexation of glucan chains into the enzyme active site and excessive enzyme jamming along the crowded substrate. Jamming could be alleviated by increasing the decomplexation rate constant but at the expense of reduced processivity. We demonstrate that enhancing the apparent reaction rate required a subtle balance between accelerating the complexation driving force and simultaneously avoiding enzyme jamming. Via a spatiotemporal systems analysis, we developed a unified mechanistic framework that delineates the experimental conditions under which different sets of rate-limiting behaviors emerge. We found that optimization of the complexation-exchange kinetics is critical for overcoming the barriers imposed by interfacial confinement and accelerating the apparent rate of enzymatic cellulose decomposition.</P>
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Ya-Ting Shang,Chu-Min Liao,Diane L. Gill 숙명여자대학교 아시아여성연구원 2012 Asian Women Vol.28 No.3
The purpose of this study was to examine sport gender ideology (belief that men in sport have to show their masculinity and women their femininity) and past contact experiences as predictors of coaches’ and athletes’ attitudes toward sexual minority athletes. Surveys were completed by 315 athletes and 94 coaches from college varsity teams in Taiwan. Hierarchical regression results showed that, for male athletes and female coaches, lower levels of sport gender ideology and positive previous experiences with homosexuals predicted more positive attitudes toward sexual minority athletes. For female athletes and male coaches, positive experiences with sexual minorities were associated with a more positive attitude toward gay and lesbian athletes. The different results may reflect position expectations/role constraints that lead male athletes and female coaches to abide by masculinity-dominated formal and informal rules. For female athletes, attitudes toward sexual minority athletes may be a self-expressive function of other values, such as justice.
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Zhi-hao Li,Shang Chu,Ping Wang,Sihai Gao,Shu-gang Li,Xiongwei Yu 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.9
This study examined the effects of differentdoses of irradiation treatments on protein structure anddigestion characteristic of seed-watermelon seed kernelprotein. The results showed that, the molecular structure ofseed-watermelon kernel protein was unfolded after theirradiation treatment, the content of b-sheet structure in thesecondary structure was decreased, while the content ofrandom coil structure increased. The average particle sizeof the protein increased, and the hydrophobic group buriedin the b-sheet structure was exposed hence the surfacehydrophobicity increased. Besides, the surface morphologyof seed-watermelon protein changed from smooth and flatto coarse and concave, the specific surface area in contactwith the aqueous medium increased and its solubilityincreased, the distribution of peptides in the digestabecame wider, and the small molecular weight peptidesgradually increased.
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Li, Chang-Zhi,Chien, Shang-Chieh,Yip, Hin-Lap,Chueh, Chu-Chen,Chen, Fang-Chung,Matsuo, Yutaka,Nakamura, Eiichi,Jen, Alex K.-Y. Royal Society of Chemistry 2011 Chemical communications Vol.47 No.36
<P>A facile synthesis was employed to make a 56π-electron methano-PC<SUB>61</SUB>BM with a very small 1,2-dihydromethano (CH<SUB>2</SUB>) group. This new fullerene derivative possesses high electron mobility (0.014 cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP>) and higher LUMO energy level (0.15 eV) than PC<SUB>61</SUB>BM. Bulk hetero-junction devices based on using poly(3-hexylthiophene) and methano-PC<SUB>61</SUB>BM as active layer exhibited better performance and thermal stability than those using the PC<SUB>61</SUB>BM analogue.</P> <P>Graphic Abstract</P><P>We have designed and synthesized a new 56π-electron methano-PC<SUB>61</SUB>BM using a very small and stable 1,2-dihydromethano (CH<SUB>2</SUB>) group. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1cc14446d'> </P>
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Mao Jianxin,Wang Di,Wang Dong,Wu Qi,Shang Qiliang,Gao Chu,Wang Huanbo,Wang Han,Du Mu,Peng Pandi,Jia Haoruo,Xu Xiaolong,Wang Jie,Yang Liu,Luo Zhuojing 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Mitochondrial dysfunction plays a major role in the development of intervertebral disc degeneration (IDD). Sirtuin 5 (SIRT5) participates in the maintenance of mitochondrial homeostasis through its desuccinylase activity. However, it is still unclear whether succinylation or SIRT5 is involved in the impairment of mitochondria and development of IDD induced by excessive mechanical stress. Our 4D label-free quantitative proteomic results showed decreased expression of the desuccinylase SIRT5 in rat nucleus pulposus (NP) tissues under mechanical loading. Overexpression of Sirt5 effectively alleviated, whereas knockdown of Sirt5 aggravated, the apoptosis and dysfunction of NP cells under mechanical stress, consistent with the more severe IDD phenotype of Sirt5 KO mice than wild-type mice that underwent lumbar spine instability (LSI) surgery. Moreover, immunoprecipitation-coupled mass spectrometry (IP-MS) results suggested that AIFM1 was a downstream target of SIRT5, which was verified by a Co-IP assay. We further demonstrated that reduced SIRT5 expression resulted in the increased succinylation of AIFM1, which in turn abolished the interaction between AIFM1 and CHCHD4 and thus led to the reduced electron transfer chain (ETC) complex subunits in NP cells. Reduced ETC complex subunits resulted in mitochondrial dysfunction and the subsequent occurrence of IDD under mechanical stress. Finally, we validated the efficacy of treatments targeting disrupted mitochondrial protein importation by upregulating SIRT5 expression or methylene blue (MB) administration in the compression-induced rat IDD model. In conclusion, our study provides new insights into the occurrence and development of IDD and offers promising therapeutic approaches for IDD.
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