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Optimal Controller for Near-Space Interceptor with Actuator Saturation
FAN Guo-long,LIANG Xiao-geng,HOU Zhen-qian,YANG Jun 한국항공우주학회 2013 International Journal of Aeronautical and Space Sc Vol.14 No.3
The saturation of the actuator impairs the response performance of the near space interceptor control system. A control system based on the properties of linear tracking system is designed for this problem. The properties are that the maximum value of the pseudo-Lyapunov function of the linear tracking control system do not present at the initial state but at the steady state, based on which the bounded stability problem is converted into linear tracking problem. The pseudo-Lyapunov function of the linear tracking system contain the input variables; the amplitude and frequency of the input variables affect the stability of the nonlinear control system. Designate expected closed-loop poles area for different input commands and obtain a controller which is function of input variables. The coupling between variables and linear matrices make the control system design problem non-convex. The non-convex problem is converted into a convex LMI according to the Shur complement lemma and iterative algorithm. Finally the simulation shows that the designed optimal control system is quick and accurate; the rationality of the presented design techniques is validated.
A Coumarin-based Fluorescent Sensor for Selective Detection of Copper (II)
Wang, Jian-Hong,Guo, Xin-Ling,Hou, Xu-Feng,Zhao, Hui-Jun,Luo, Zhao-Yang,Zhao, Jin Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.8
Cu (II) detection is of great importance owing to its significant function in various biological processes. In this report, we developed a novel coumarin-based chemosensor bearing the salicylaldimine unit (2) for $Cu^{2+}$ selective detection. The results from fluorescence spectra demonstrated that the sensor could selectively recognize $Cu^{2+}$ over other metal cations and the detection limit is as low as $0.2{\mu}M$. Moreover, the confocal fluorescence imaging in HepG2 cells illustrated its potential for biological applications.
Correlation Between EGFR Mutations and Serum Tumor Markers in Lung Adenocarcinoma Patients
Pan, Jin-Bing,Hou, Yu-Hong,Zhang, Guo-Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2
Background: Mutations affecting the epidermal growth factor receptor (EGFR) are good predictors of clinical efficacy of EGFR tyrosine kinase inhibitors (TKI) in patients with non-small cell lung cancer. Serum carcinoembryonic antigen (CEA) levels are also regarded as predictive for the efficacy of EGFR-TKI and EGFR gene mutations. This study analyzed the association between EGFR gene mutations and clinical features, including serum tumor marker levels in lung adenocarcinomas patients. Patients and Methods: A total of 70 lung adenocarcinoma patients with complete clinical data and pathological specimens were investigated. EGFR gene mutations at exons 19 and 21 were assessed. Serum tumor markers were detected by protein chip-chemiluminescence at the corresponding time, and correlations were analyzed. Results: Mutations of the EGFR gene were detected in 27 of the 70 patients and the serum CEA and CA242 concentrations were found to be significantly associated with the incidence of EGFR gene mutations (P<0.05). The AUCs for CEA and CA242 were 0.724 (95% CI: 0.598~0.850, P<0.05) and 0.769 (95% CI: 0.523~0.800, P<0.05) respectively. Conclusions: Serum CEA and CA242 levels are associated with mutations of the EGFR gene in patients with lung adenocarcinomas.
Yong-Qiang Zhao,Guo-Di Liu,Cong-Cong Hou,Ying-Li Han,Jun-Quan Zhu,Jun-Quan Zhu 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.3
Catalase (CAT) is an essential antioxidant in organisms, its can eliminate hydrogen peroxides produced in the cellular environment. Previous studies have focused mainly on the functions of CAT by pathogen challenge, but the functions of CAT by metals stress remain unclear. In order to investigate the function of CAT in Acrossocheilus fasciatus (AfCat), we cloned CAT complete cDNA sequences and analyzed its functions by cadmium (Cd) stress. qPCR analysis illustrated that the expression of CAT changed rapidly and dynamically in response to Cd from 0.05 mg L-1 to 1.25 mg L-1. Enzyme activity assay result showed that CAT activity was up-regulated significantly in response to Cd from 0.05 mg L-1 to 1.25 mg L-1. Histochemistry analysis showed that the structure of liver, testis and kidney were severely damaged by exposed to Cd of 1.25 mg L-1. Collectively, the results of our study indicate that AfCat may play an indispensable role in maintaining redox balance under Cd exposure, but AfCat expression will be inhibited when Cd accumulation reaches to the critical concentration. This study will illuminate the potential functions of AfCat in the defense mechanisms of reproductive and metabolic systems under Cd exposure in A. fasciatus.
Yu Jian,Li Wen,Hou Guo-jun,Sun Da-peng,Yang Yuan,Yuan Sheng-xian,Dai Zhi-hui,Yin Hao-zan,Sun Shu-han,Huang Gang,Zhou Wei-ping,Yang Fu 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Hepatitis B protein x (HBx) has been reported to promote tumorigenesis in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), but the mechanism awaits further investigation. In this study, we found that cFAM210A (a circular RNA derived from the third exon of transcript NM_001098801 of the FAM210A gene; CircBase ID: hsa_circ_0003979) can be silenced by HBx. cFAM210A expression was downregulated and negatively correlated with tumorigenesis in patients with HBV-related HCC. Furthermore, cFAM210A reduced the proliferation, stemness, and tumorigenicity of HCC cells. Mechanistically, HBx increased the N6-methyladenosine (m6A) level of cFAM210A by promoting the expression of RBM15 (an m6A methyltransferase), thus inducing the degradation of cFAM210A via the YTHDF2-HRSP12-RNase P/MRP pathway. cFAM210A bound to YBX1 and inhibited its phosphorylation, suppressing its transactivation function toward MET. These findings suggest the important role of circular RNAs in HBx-induced hepatocarcinogenesis and identify cFAM210A a potential target in the prevention and treatment of HBV-related HCC.
A Coumarin-based Fluorescent Sensor for Selective Detection of Copper (II)
Jian-Hong Wang,Xin-Ling Guo,Xu-Feng Hou,Hui-Jun Zhao,Zhao-Yang Luo,Jin Zhao 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.8
Cu (II) detection is of great importance owing to its significant function in various biological processes. In this report, we developed a novel coumarin-based chemosensor bearing the salicylaldimine unit (2) for Cu2+ selective detection. The results from fluorescence spectra demonstrated that the sensor could selectively recognize Cu2+ over other metal cations and the detection limit is as low as 0.2 μM. Moreover, the confocal fluorescence imaging in HepG2 cells illustrated its potential for biological applications.
IRS-2 Partially Compensates for the Insulin Signal Defects in IRS-1−/−Mice Mediated by miR-33
Chen-Yi Tang,Xiao-Fei Man,Yue Guo,Hao-Neng Tang,Jun Tang,Ci-La Zhou,Shu-Wen Tan,Min Wang,Hou-De Zhou 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.2
Insulin signaling is coordinated by insulin receptor substrates (IRSs). Many insulin responses, especially for blood glucose metabolism, are mediated primarily through Irs-1 and Irs-2. Irs-1 knockout mice show growth retardation and insulin signaling defects, which can be compensated by other IRSs in vivo; however, the underlying mechanism is not clear. Here, we presented an Irs-1 truncated mutated mouse (Irs-1−/−) with growth retardation and subcutaneous adipocyte atrophy. Irs-1−/− mice exhibited mild insulin resistance, as demonstrat-ed by the insulin tolerance test. Phosphatidylino-sitol 3-kinase (PI3K) activity and phosphorylated Protein Kinase B (PKB/AKT) expression were elevated in liver, skeletal muscle, and subcu-taneous adipocytes in Irs-1 deficiency. In addition, the expression of IRS-2 and its phosphorylated version were clearly elevated in liver and skeletal muscle. With miRNA microarray analysis, we found miR-33 was down-regulated in bone marrow stromal cells (BMSCs) of Irs-1−/− mice, while its target gene Irs-2 was up-regulated in vitro studies. In addition, miR-33 was down-regulated in the presence of Irs-1 and which was up-regulated in fasting status. What’s more, miR-33 restored its expression in re-feeding status. Meanwhile, miR-33 levels decreased and Irs-2 levels increased in liver, skeletal muscle, and subcutaneous adipocytes of Irs-1−/− mice. In primary cultured liver cells transfected with an miR-33 inhibitor, the expression of IRS-2, PI3K, and phosphorylated-AKT (p-AKT) increased while the opposite results were observed in the presence of an miR-33 mimic. Therefore, decreased miR-33 levels can up-regulate IRS-2 expression, which appears to compensate for the defects of the insulin signaling pathway in Irs-1 deficient mice.
Jiang, Xiao-Ting,Ma, Ying-Yu,Guo, Kun,Xia, Ying-Jie,Wang, Hui-Ju,Li, Li,He, Xu-Jun,Huang, Dong-Sheng,Tao, Hou-Quan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21
Background: This study aimed to summarize the potential diagnostic value of serum DKK1 levels in cancer detection. Materials and Methods: Serum DKK1 was measured using enzyme-linked immunosorbent assay in a case-control study. Then we performed a meta-analysis and the pooled sensitivity, specificity, diagnostic odds ratio, and summary receiver operating characteristic (sROC) curves were used to evaluate the overall test performance. Results: Serum DKK1 levels were found to be significantly upregulated in gastric cancer as compared to controls. ROC curve analysis revealed an AUC of 0.636, indicating the test has the potential to diagnose cancer with poor accuracy. The summary estimates of the pooled sensitivity, specificity and diagnostic odds ratio in meta-analysis were 0.55 with a 95% confidence interval (CI) (0.53-0.57), 0.86 (95%CI, 0.84-0.88) and 12.25 (95%CI, 5.31-28.28), respectively. The area under the sROC was 0.85. Subgroup analysis revealed that the diagnostic accuracy of serum DKK1 in lung cancer (sensitivity: 0.69 with 95%CI, 0.66-0.74; specificity: 0.95 with 95%CI, 0.92-0.97; diagnostic odds ratio: 44.93 with 95%CI, 26.19-77.08) was significantly higher than for any other cancer. Conclusions: Serum DKK1 might be useful as a noninvasive method for confirmation of cancer diagnosis, particularly in the case of lung cancer.