행인 제제의 독성 및 항암효과와 자연살해세포 활성에 미치는 효과

심범상,최승훈,박재경 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1

In this experiment, we examined the the effect of Laetrile Oil to the immune system and tried to disclose the anti-cancer mechanism of this. The result is listed as below. 1. After per-os, sub-cutaneous and into the peritonal injection of Laetrile Oil to the SPF mice, the LD50 is above 5ooomg/kg at even roup. 2. Mean survival days of mice treated with laetril oil, after S-180 cells transplantation into the eritoneal cavoty decreases 1.5 days(-6.8%) compared with the Mean survival days of the control group(22days.) 3. Effects of laetril oil on natural killer cell activity at Effector/Target Cell Ratio with 100:1, 50:1, l0:l into methotrexate-pretreated mice is like this.: Compared with 29.22±l2.7% Cytotoxicity of the control group, sample group's Cytotoxicity had 38.83±l2.5% of meaningful acrease. At l00:1 Effecr/Target Cell Ratio. At 50:l Effector/Target Cell Ratio, control group has 20.02±9.6% Cytotoxiciy and sample group had 3l.53±13.4% Cytotoxicity. At 25:l Effector/Target Cell Ratio, control group has 13.60±6.6% Cytotoxicity and sample group had 20.8l±9.8% Cytotoxicity. According to the above results, the Laetrile Oil represents nontotoxic to a SPF mice. non-effective to transplantable Sarcoma 180 tumors, and activaion in NK cell activity.

「마키버」의 多元的 國家論의 對한 硏究

愼性範 中央大學校 法政大學 學生聯合學會 1960 法政論叢 Vol.10 No.-

HMM에 의한 연속음성 인식 시스템의 구현

심장엽,김상범,김주성,김수훈,이영재,허강인 東亞大學校 附設 情報通信硏究所 1996 情報通信硏究所論文誌 Vol.4 No.1

This paper is a study on the composition of Real-Time Continuous Speech Recognition System for Man-Machine Interface and it examines the posibility that applies to automatic system. The Continous Speech Recognition System is composed of Contivuous Distribution HMM model and algorithm of One Pass DP method. The System is composed so that it may detect start point and end point of speech data which are converted into samples by 10 KHz, 8 bit A/D whthin real time, then so that it may recognie them by one Pass DP method, display the result of recognition on PC monitor and at same time sent control data to Interface. HMM models are creadted by training for continuous speech samples which are control words, area names and digital sounds. In the result of experiment by Continuous Speech Recognition System, there are some kind of errors which are insertion, replacement and deletion of one syllable, but it examined the posibility that can by applied to Man-Machine Interface on automatic system if post-process is performed for recognition.

정형외과 영역에서 Sulperazon의 임상적 경험

심대무,나범수,김상수 최신의학사 1991 最新醫學 Vol.34 No.7

란크릭 캅셀(플루옥세틴 20mg)에 대한 프로작 캅셀의 생물학적 동등성 시험

심상범,조요나,오한석,류재환,이경태,김남재,서성훈 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

Bioequivalence of two flouxetine capsule, ProzacR (Lilly kora Ltd.) and LanclicR (Samsung Pharm. IND. Co.), was evalated according to the guideline of KFDA. Twenty four healthy male volunteers (21-26 years old) were divided into two groups and a randomized 2×2 cross-over study was employed. After 60mg of fluoxetine was orally administered, blood was taken at 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, 24, 32, 48 and 72 hours after administration and just before administration. Plasma was analyzed for fluoxetine and internal standard (clomipramine) by a sensitive and validated HPLC assay. The pharmaco kinetic parameters (AUCt, Cmax and Tmax) wre calculated and ANOVA test was used for the statistical analysis of parameters. Differences in (AUCt, Cmax and Tmax between two capsules were -0.90, 3.46 and -14.08% respectively. All powers (1-β) for AUGt, Cmax and Tmax were more than 0.9. Detectable differences (Δ) and confidence interval were all less than ±20%. All the parameters above met the criteria of KFDA for bioequivalence and indicated that LanclicR capsules are bioequivalent to ProzacR capsules.

대장암조직에서 아포토시스 검출 방법에 관한 연구

김광호,심강섭,김성숙,구혜수,박응범 梨花女子大學校 醫科大學 醫科學硏究所 1997 EMJ (Ewha medical journal) Vol.20 No.2

1989년 1월부터 1992년 12월 사이에 대장암으로 근치적 절제술을 시행받은후 추적조사가 가능한 45명의 환자를 대상 대장암조직의 Hematoxylin-Eosin염색을 통하여 apoptotic body를 광학 현미경하에서 400배율 하에서 관찰하여 암세포 100개당 평균개수를 세었으며 TUNEL 방법을 이용하여 면화학적염색을 통하여 염색정도를 판정을하여 대장암에서 아포토시스의 정도를 판정하여 다음과 같은 결과를 얻었다. Dukes병기 A 일 때 apoptotic body가 평균 1.93개, B 일 때 2.66개, C 인 경우 2.76개 였으며 분화도에 따라서는 고분화암인 경우 2.51개, 중등도분화암인 경우는 3.01개, 저분화암인 경우는 2.15개였다. 5년내 사망한 환자에서는 3.55개 였으며 5년이상 생존한 환자에서는 2.8개였다. 형태학적으로 apoptotic body의 수를 세어 아포토시스를 측정하여 예후예측인자로 사용할 수 있으리라 사료되며 아포토시스와 p53의 발현과의 관계를 보았을 때 특별한 상관관계를 발견 할 수 없어 bcl-2의 발현과 더불어 아토토시스와의 관게에 대하여 좀더 연구가 되어야 하겠다. Objectives : Apoptosis is a specific mode of cell death recognized by a characteristic pattern of morphological, biochemical, and molecular changes, There are several methods of detection of apoptosis. Morphological changes involve a characteristic pattern of chromation and cytoplasm. The landmark of apoptosis is endonucleolysis, with nuclear DNA initially degraded at the linker sections to fragments equivalent to single and multiple nucleosomes. Detection of DNA fragments is situ using the terminal deoxyribonucleotidyl transferase(TDT)-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay is increasingly applied to investigate apoptosis. We studied the detection method of apoptosis morphologically and by using TUNEL assay and examined the correlation of p53 expression and apoptosis. Methods : Forty-five cases of colorectal cancer were selected. The number of apoptotic bodies was expressed as a number per 100 cancer cells. The TUNEL assay was performed with in situ Apoptag kit®. Results : The mean number of the apoptotic bodies was 2.28 in the patients who survived over 5 years after curative resection and 3.55 in the patients who died within 5 years(p=0.001). There was a relationship between the number of apoptotic bodies which were measured by morphologic study and the results which were measured by TUNEL assay. There was no relationship between p53 expression and apoptosis. Conclusion : These results suggest that the frequency of apoptotic bodies may be a prognostic factor for colorectal cancer and apoptosis could be measured by morphological study without special study.

血府逐瘀湯이 癌轉移 抑制에 미치는 影響

李眞華,沈範相,安圭錫,崔昇勳 대한한방종양학회 1999 대한한방종양학회지 Vol.5 No.1

To examine the effect of XuehdRUIng on the metastasis of cancer, the following experiments were carried out. Before the main experimnts, the cytotoxicity was measured by putting Xuefuzhuyutant sample in HT1080. Then zymography was made to examine the change of gelatinolytic activity. Weste blotting was carried out to examine the changes of Fos, Jun, Ets, Erk, md JNK. In vitro invasion assay with transwells coated by collagen and mangel was carried out. From the above reams the following conclusions were obtained. 1. The eXperimental result about cytotoxicity of Xuefuzhuyutang agaitst HTlO8O was a below. The stained cell count after beingtreated by by Xuefuzhuyutang sample 4M㎍/㎖ for 24 hours was 0.9% of total cells, and the stained cell count by Xuefuzhuyutang sample 100㎍/㎖ was 1.5% of total cells. Both were near the level of control group which showed O.6% stained. 2. Thee result of collagenase assay was as below. In Xuefuzhuyutang sample 400㎍/㎖, MMP2 was reduced as compared with TPA control group, and the band of MMP-9 induced by TPA disapappeared. In Xuefuzhuyutang samle 800㎍/㎖ both bands of MMP-2 and MMP-9 diSamared. 3. The results of westem blots for Jun, Fos, Ets, Erk, JNK were a below. In Xuefuzhuyutang sample 200㎍/㎖, Ets was reduced, and Jun, Fos were increased. 4 The result of invasion assay was as below. The number of cells which migrated gross trayswell membrane in Xuefuzhuyutang-treated group was less than that of control(+TPA) group. From the above results, it was concluded that Xuefuzhuyutang might inhibit the activity of collagenase not by the MMP-2, MMP-9 promoter but by the Other way.

胃癌에서의 辨證分型에 關한 文獻的 考察

崔昇勳,沈範相 대한동의병리학회 1993 동의생리병리학회지 Vol.8 No.-

연구배경 : 최근 생활상의 변화에 따른 종양의 발생빈도가 증가하고 있으며 이에 따른 각종 임상보고 및 실험보고가 활발하게 이뤄지고 있으나 암에 대한 동서의학간의 인식과 접근의 차이에 따라 실제 임상상에서 많은 혼란이 일고 있다. 가령 胃癌의 경우 이에 해당하는 한의학적 병증으로는 積聚, 비하, 현벽, 反胃, 伏梁, 胃脘通, 심하비 등이 거론될 수 있으며 각각의 변증분형까지 살펴본다면 더욱 혼란스럽다. 이에 따라 본 논문에서는 위암의 변증분형을 예로 삼아 문헌적인 고찰을 통해 그 규범화 가능성을 살펴보았다. 방법 : 위암의 주된 증상중에서 한의학적 병증으로 대표적이라고 생각되는 위완통, 반위의 병인병기 및 변증분형을 고찰하였고 이를 현대 중국의 중서외결합에 의한 변병의 기초위에서 행해진 위암의 변증분형과 상호 비교하였다. 결과 : 병인병기 방면에서는 대개 위암, 위완통, 반위가 별 차이없이 중복되고 있으나 변증분형에서는 위완통의 경우 氣滯(肝胃不和)만이, 반위의 경우 反胃初氣를 제외한 脾胃虛寒, 命門火衰, 眞陰枯虧, 酒濕傷脾 등이 위암의 변증분형과 대체로 일치하고 있다. 결론 : 위완통은 早期胃癌에, 반위는 進行癌 혹은 胃癌의 末期에 상당하는 것으로 생각된다. 위암의 발생과 발전 등에 따른 病期別로 해당하는 한의학적 병증들에 대한 문헌적 비교고찰이 필요하며 보다 심화된 연구와 변증분형의 규범화를 위해서는 정확한 임상적 보고가 필요한 것으로 생각된다.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

혈관내초음파로 평가한 스텐트를 삽입한관동맥의 형태학적 특징

정남식,홍범기,임세중,백성일,이문형,장양수,심원흠,조승연,김성순 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.8