Contact toxicity and transcriptomic analysis of terpinen-4-ol exposure in Tribolium castaneum

Shan-shan Gao,Yong-lei Zhang,Kun-peng Zhang,Wang Xing-yun,Qing-bo Tang,Yuan-chen Zhang 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3

The terpene, terpinen-4-ol (T4ol), exhibits contact toxicity in Tribolium castaneum. However, the molecular mechanisms underlying this toxicity have not been elucidated. This study examined changes in the expression of four classic enzymes after exposure of T. castaneum to T4ol. Acetylcholinesterase and glutathione S-transferase activities were markedly inhibited after exposure to T4ol, while that of the detoxifying enzyme cytochrome oxidase P450 increased markedly. Carboxylesterase activity did not show significant changes. Furthermore, RNA sequencing revealed 260 differentially expressed genes (DEG) between the T4ol-treated and control samples, and qRT-PCR was used to validate the RNA-Seq data. The Gene Ontology analysis classified the DEGs into 36 functional groups, including the immune system processes, response to stimulus, and developmental processes. T4ol altered the response to stimulus and the immune system process of beetles by inducing the expression of the genes Stabilin-1, Attacin 1, and Defensin 1. Furthermore, the DEGs receptor tyrosine kinase Torso-like protein (RTKTsl), Frizzled 4 (Fz4), Protein Wnt-5b, Ecdysone-induced protein 78C (E78), Zinc finger protein GLIS1 (ZFPGLIS1) were classified as participating in beetle development, and Fz4 and Protein Wnt-5b also mapped to the Wnt signaling pathway. This indicated that pathways associated with development are inhibited after exposure to T4ol. T4ol also induced CYP9Z6/GSTs7 overexpression, and RNAi targeting these genes significantly increased larvae mortality on T4ol exposure, supporting the participation of CYP9Z6/GSTs7 in the response to T4ol in T. castaneum. The results of this study will facilitate understanding of the toxic mechanisms of T4ol and provide a basis for controlling the pests of stored products.

Density Functional Study on [3+2]-Dipolar Cycloaddition Reaction of the N-heterocyclic Carbene Boryl Azide with Olefins

Xing-hui Zhang,Ke-tai Wang,Teng Niu,Shan-shan Li 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.5

The cycloaddition reactions of the N-heterocyclic carbene boryl azide with methyl acrylate, butenone, and hexafluoropropene have been investigated theoretically. Solvent effects on these reactions have been explored by calculation that included a polarizable continuum model (PCM) for the solvent (C6H6). The title reaction could produce 4- and 5-substituted 1,2,3-triazolines, respectively. The reaction systems have the higher chemical reactivity with the low barriers and could be favored. Yet the smaller differences have been found to occur in energetics, and the cycloaddition reactions occur for s-trans conformations over s-cis conformations. The calculations indicated that the cycloaddition reaction of the alkenes have certain regioselectivity.

Adipose Stem Cells with Conditioned Media for Treatment of Acne Vulgaris Scar

Xing Shan,최종현,김기주,이윤재,류연희,이수진,문석호,이종원 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.1

This study was to investigate the effect of subcutaneous injection of the adipose stem cells (ASCs) with conditioned media (CM) in the treatment of acne vulgaris scar. We used Adult male New Zealand white rabbit ears as an animal model and induced acne formation by Kignman method. Adipose tissue was isolated and harvested from the scapula of rabbits, and ASCs were cultured and expanded until passage 1. There have four groups in our experiment, include phosphate buffered saline (PBS), ASCs with PBS (ASC ? PBS), CM, and ASCs with CM (ASC ? CM) group. This solution of 0.6 ml injected to subcutaneous in each group. ASC ? PBS and ASC ? CM groups were containing ASCs of 5.0 9 106 cells/ml. We analyzed the treatment of 4 groups to scar tissue after 2 and 4 weeks by hematoxylin and eosin stain, immunohistochemistry, and RNA expression level of tumor necrosis factor-a (TNF-a), interleukin-1a (IL-1a), and matrix metalloproteinase-2 (MMP-2). Also, the expression of keratin 16 (K16) was detected by western blot analysis. H&E stain showed that infiltration of inflammation cells was significantly reduced at 2 and 4 weeks, as well as re-epithelialization was improved in the ASC ? CM group. The ASC ? CM gourp was reduced both expression levels of TNF-a, IL- 1a, and MMP-2 and K16 protein level. In conclusion, the ASCs with CM has a significant curative effect on acne vulgaris scar, more to the point, the CM has a key role on treatment. It could be applied to a therapeutic approach to regenerate to treat acne vulgaris scar.

Density Functional Study on [3+2]-Dipolar Cycloaddition Reaction of the N-heterocyclic Carbene Boryl Azide with Olefins

Zhang, Xing-Hui,Wang, Ke-Tai,Niu, Teng,Li, Shan-Shan Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.5

The cycloaddition reactions of the N-heterocyclic carbene boryl azide with methyl acrylate, butenone, and hexafluoropropene have been investigated theoretically. Solvent effects on these reactions have been explored by calculation that included a polarizable continuum model (PCM) for the solvent (C6H6). The title reaction could produce 4- and 5-substituted 1,2,3-triazolines, respectively. The reaction systems have the higher chemical reactivity with the low barriers and could be favored. Yet the smaller differences have been found to occur in energetics, and the cycloaddition reactions occur for s-trans conformations over s-cis conformations. The calculations indicated that the cycloaddition reaction of the alkenes have certain regioselectivity.

Higher-level Production of Ascomycin (FK520) by Streptomyces hygroscopicus var. Ascomyceticus Irradiated by Femtosecond Laser

Hai-shan Qi,Xing Xin,Shan-shan Li,Jian-ping Wen,Yun-lin Chen,Xiao-qiang Jia 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.4

Femtosecond laser irradiation technology was employed for the first time to improve the ascomycin (FK520)yield of Streptomyces hygroscopicus var. ascomyceticus NT2-11, which is an N-methyl-N-nitro-N-nitrosoguanidine (NTG)-induced strain derived from S. hygroscopicus (ATCC14891). The mutant FS35 with high and stable FK520 production capacity was then obtained in the optimal irradiation conditions (25 mW for 6 min) by the Titanium sapphire laser system (810 nm, 76 MHz, 150 fs). The FK520 production capacity of FS35 was 45% higher than that of the parental strain NT2-11. Moreover, under the optimal fermentation conditions, FK520 fermentation titer of FS35 reached 300 mg/L and the intrinsic kinetics of FS35 and NT2-11 were investigated comparatively in 3phases. The mathematical models provided a good description of FK520 fermentation process for both strains and valuable information for optimizing operation and pilotplant enlargement research. The comparative studies on parameters of the models confirmed the advantages in production and the decrease of substrate inhibition through femtosecond laser irradiation. Therefore, femtosecond laser irradiation provides a promising way to enhance the production of FK520 in S. hygroscopicus. Femtosecond laser irradiation technology was employed for the first time to improve the ascomycin (FK520)yield of Streptomyces hygroscopicus var. ascomyceticus NT2-11, which is an N-methyl-N-nitro-N-nitrosoguanidine (NTG)-induced strain derived from S. hygroscopicus (ATCC14891). The mutant FS35 with high and stable FK520 production capacity was then obtained in the optimal irradiation conditions (25 mW for 6 min) by the Titanium sapphire laser system (810 nm, 76 MHz, 150 fs). The FK520 production capacity of FS35 was 45% higher than that of the parental strain NT2-11. Moreover, under the optimal fermentation conditions, FK520 fermentation titer of FS35 reached 300 mg/L and the intrinsic kinetics of FS35 and NT2-11 were investigated comparatively in 3phases. The mathematical models provided a good description of FK520 fermentation process for both strains and valuable information for optimizing operation and pilotplant enlargement research. The comparative studies on parameters of the models confirmed the advantages in production and the decrease of substrate inhibition through femtosecond laser irradiation. Therefore, femtosecond laser irradiation provides a promising way to enhance the production of FK520 in S. hygroscopicus.

Introducing Network Situation Awareness into Software Defined Wireless Networks

( Xing Zhao ),( Tao Lei ),( Zhaoming Lu ),( Xiangming Wen ),( Shan Jiang ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.3

The concept of SDN (Software Defined Networking) endows the network with programmability and significantly improves the flexibility and extensibility of networks. Currently a plenty of research works on introducing SDN into wireless networks. Most of them focus on the innovation of the SDN based architectures but few consider how to realize the global perception of the network through the controller. In order to address this problem, a software defined carrier grade Wi-Fi framework called SWAN, is proposed firstly. Then based on the proposed SWAN architecture, a blueprint of introducing the traditional NSA (Network Situation Awareness) into SWAN is proposed and described in detail. Through perceiving various network data by a decentralized architecture and making comprehension and prediction on the perceived data, the proposed blueprint endows the controllers with the capability to aware of the current network situation and predict the near future situation. Meanwhile, the extensibility of the proposed blueprint makes it a universal solution for software defined wireless networks SDWNs rather than just for one case. Then we further research one typical use case of proposed NSA blueprint: network performance awareness (NPA). The subsequent comparison with other methods and result analysis not only well prove the effectiveness of proposed NPA but further provide a strong proof of the feasibility of proposed NSA blueprint.

Tuning the Mechanical Properties of Silkworm Silk Fibres by Thermally Induced Modification of Crystalline Nanostructure

Shan Du,Weitao Zhou,Xing Jin,Yimin Zhang,Xiangxiang Chen,Jin Zhang,Jingliang Li,Xungai Wang 한국섬유공학회 2021 Fibers and polymers Vol.22 No.2

Antheraea pernyi and Bombyx mori silk fibres, the most important and the subjects of several studies, have attractedwidespread attention due to their exceptional mechanical properties and promising applications. However, the determinantsbehind the variations of mechanical property between these two fibres still remains unknown. In this paper, the nanocrystallinestructures of these two silk fibres were tuned by thermal treatment in order to understand how the structure affects themechanical properties of fibres. It has been found that, along with an increase in temperature for thermal treatment, theincrease in the intramolecular β-sheet leads to progressive increase in longitudinal modulus and radial hardness, whilst thereduction in crystallinity results in great reduction in breaking tensile strength. The yield strength is proportional to theaverage crystallite size which increases along with the annealing temperature. Compared to B. mori, A. pernyi silk fibres havemore obvious temperature-dependant structural changes. These findings explain how size effects can be exploited to createbioinspired materials with tuneable mechanical properties.

Experimental and Theoretical Investigation on Flexural Behavior of Corroded Steel Beams Strengthened by CFRP Plate

Zong-Xing Zhang,Shan-Hua Xu,Lin Mu,Sheng-Yuan Peng 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.7

To investigate the flexural behavior of corroded steel beams strengthened by carbon fiber reinforced polymer (CFRP) plate, flexural test of five steel beams were carried out. The effects of corrosion and prestressing force levels on the flexural capacity, failure modes and interfacial stress were investigated. Meanwhile, considering the corrosion surface the calculation method of flexural capacity of corroded beams strengthened by CFRP plate was also established. The results showed that failure modes of corroded beams were the fracture of the CFRP plate after the shear failure of interface on the mid-span and fracture location of CFRP plate was mostly at the loading point. The rough surface of the corroded steel can enhance the efficiency of stress transfer at the interface, thereby improving the effective bond length of the interface. The shear stress was mainly concentrated on the CFRP plate end, and peak value appeared at the loading point. Compared the reference beam, the ultimate flexural capacity of the corroded beam strengthened by CFRP plate with 15% prestress level increased at a ratio of 21%, and utilization ratio of the CFRP plates was up to 71.59%.

Microstructure Distribution and Grain Coarsening Model of GCr15 Steel in the Laser Surface Treatment

Jin‑shan Chen,Zhen‑xing Li,Ya‑jie Chu,Jie Chen, Xin‑jun Shen 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.10

The GCr15 steel was subjected to laser surface treatment using a diode laser with rectangular spot. Based on the simulatedtemperature field, the microstructure distribution of the laser hardened layer was analyzed, and an empirical equation wasproposed to predict the peak temperature at different depths. The results indicated that with the decrease of depth, the peaktemperature increased exponentially, and the peak temperature at different depths was approximately linear with the powerdensity and the reciprocal of square root of scanning speed. High peak temperature led to the coarsening of grain and thesignificant dissolution of cementite near the surface of hardened layer. At the deeper position of hardened layer, the prioraustenite grain size decreased slightly due to high nucleation rate. The cementite dissolution and coarsening occurred simultaneouslynear the surface, and the cementite dissolution process was postponed due to the significant enrichment of Cr nearthe interface of austenite and cementite. Additionally, an empirical model about grain coarsening was established, which canbe used to predict the prior austenite grain size near the surface during the laser surface treatment.

Telecommunication Method for Single Chip Microcomputers

Bing Shan Lin,Zu Xing,Hui Feng Li 대한전자공학회 1991 International Conference on Electronics, Informati Vol.1 No.1