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Lee, Seungwoo,Kang, Hong Suk,Park, Jung‐,Ki WILEY‐VCH Verlag 2011 Advanced functional materials Vol.21 No.10
<P>A principal challenge in nanopatterning is to beat the resolution limit of established methods. On page 1770, Seungwoo Lee, Hong Suk Kang, and Jung‐Ki Park present the directional photofluidization of azopolymers, which allows the generation of large‐area, highly ordered nanoarchitectures with precisely controlled structural features. Additionally, this approach can offer unprecedented flexibility in control of the final feature size, and, simultaneously, its fluidic behavior under light irradiation signifi cantly reduces the line‐edge roughness </P>
Seungwoo Lee(이승우),Min-Kyung Jang(장민경),Nam-Young Kim(김남영),Hye-Ji Jang(장혜지),Dong-Geun Lee(이동근),Mihyang Kim(김미향),Yuck Young Kim(김육용),Sung Gu Kim(김성구),Byung Hong Yoo(유병홍),Sang-Hyeon Lee(이상현) 한국생명과학회 2010 생명과학회지 Vol.20 No.12
In order to evaluate estrogenic compounds in brown algae, an in vitro test system for the verification of estrogenic activity was applied. Fractions from ethanol extracts of each brown alga were prepared by a systematic fractionation procedure with solvents such as H₂O, hexane, butanol and methanol. Aqueous fractions of brown algae showed the highest estrogenic activities. Estrogenic activities of 500 ㎍/㎖ aqueous fractions of Undaria pinnatifida and Laminaria japonica showed almost the same strength as that of 10?? M standard solution (17β-estradiol). Furthermore, estrogenic activities of 500 ㎍/㎖ aqueous fractions of Ecklonia stolonifera and Porphyra suborbiculate represented higher activities than that of 10?? M 17β-estradiol. These observations suggest that aqueous fractions of all these brown algae are expected to possess estrogenic compounds and could be developed as estrogenic agents for postmenopausal disorder.
이승우(Seungwoo Lee),이원일(Wonll Lee),이근배(Geunbae Lee),이종혁(Jong-Hyeok Lee),박재득(Jaduk Park) 한국정보과학회 1997 한국정보과학회 학술발표논문집 Vol.24 No.2Ⅱ
본 논문에서는 자연어 질의가 가지는 특징들을 살펴보고, 범주 문법과 논리구조에 기반한 한국어 의미 분석기[권혜진 1997]를 기반으로 parsing기법을 개선하여 시스템의 속도를 향상시키는 방법을 제안한다. 또 자연어 처리의 각 단계의 분석 점수를 결합하여 애매성을 내포하고 있는 자연어 질의로부터 의미 분석의 best result를 얻는 방법을 소개하고 튜닝을 통해 그 결과를 확인한다.
Baekhee Lee,Seunghoon Lee,Hayoung Jung,Jawon Lee,Teukgyu Choi,Mina Lee,Hogeun Kim,Eunha Kim,Hojun Jeon,Youngbum Cho,Seungwoo Seo,Heecheon You 대한인간공학회 2014 대한인간공학회 학술대회논문집 Vol.2014 No.11
Objective: The present study is to evaluate satisfaction of vehicle outside door handles (ODHs) by design factor for improving grip comfort. Background: Existing studies have mainly focused on size and/or shape of typical handles (e.g., cylindrical handle); therefore, a specialized research for ergonomic ODH design is needed. Method: Satisfaction (operation, shape, height, width, tilting angle, central radius (R), inside upper R, inside middle R, inside lower R, pressure distribution, and overall satisfaction) by design factor (2-level: section width; 3-level: central R, inside upper R, inside middle R, inside lower R, tilting angle, and section height) were analyzed by applying a Taguchi L18 design (2¹ × 3<SUP>6</SUP>). Design levels by design factor were determined referring to 5 existing ODHs. 18 ODHs developed in the study were relatively evaluated comparing with a reference ODH (0 point) through an 11-point bipolar scale (-5: extremely dissatisfaction, 0: no difference, +5: extremely satisfaction) by twenty participants aged 20s ~ 50s. Results: Section width and inside upper R were significant on satisfaction of ODH. Conclusion: A design guideline for ODH was established considering users’ satisfaction. Application: The design factor evaluation protocol using Taguchi method applied in the study can be applicable to the other vehicle moving handles (e.g., inside door handle, door trim grip handle, door trim pull handle).
KIM, BONG CHO,YOO, HEE JUNG,LEE, HYUNG CHUL,KANG, KYOUNG-AH,JUNG, SEUNG HEE,LEE, HAE-JUNE,LEE, MINYOUNG,PARK, SEUNGWOO,JI, YOUNG-HOON,LEE, YUN-SIL,KO, YOUNG-GYU,LEE, JAE-SEON Spandidos Publications 2014 Oncology Reports Vol.31 No.5
<P>The purpose of the present study was to elucidate whether premature senescence contributes to the outcome of radiotherapy (RT) and to validate senescence biomarkers in vitro and in vivo. Cultured human cancer cell lines and xenografted mice were exposed to single (SR; 2, 6 or 12 Gy) or fractionated radiation (FR; 3 x 2 Gy or 6 x 2 Gy), and premature senescence was assessed using senescence-associated β-galactosidase (SA-β-Gal) activity, hypophosphorylation of pRb and p21 accumulation. A variety of senescence-associated biomarkers including cathepsin D (CD), the eukaryotic translation elongation factors eEF1A1, eEF1B2, decoy receptor 2 and Dec1 were further validated in vivo or in vitro. We demonstrated the beneficial tumor suppressive role of ionizing radiation (IR)-induced premature senescence in vitro and in vivo. FR inhibited tumor growth via induction of premature senescence as effectively as an equivalent SR dose (6 Gy). In addition, CD and eEF1 were valuable biomarkers of cellular senescence in either SR- or RF-exposed carcinoma cells or xenograft mice. Our results suggest that 2 Gy of a conventional RT regime could achieve a better clinical outcome if premature senescence could be increased through an improved understanding of its molecular action mechanism.</P>
Electrochemical DNA Biosensor with Nanometer Scale Using Nano - Patterning Lithography Machine
SeungWoo Lee,SooYeon Park,JaeJong Lee The Korean Society of Mechanical Engineers 2005 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.19 No.11
<P> Major challenges in the field of electrochemical DNA hybridization biosensors are the immobilization of DNA and the detection of hybridization signals. The method of DNA immobilization using the nano-patterning machine and detection for DNA hybridization signals has been proposed. Here, two gold electrodes were deposited on SiO₂ layer and the gap between the electrodes was fabricated by electron beam lithography. 3-aminopropyltriethoxysilane (APTES) solution was selectively treated to immobilize the amino-modified oligonucleotides onto the SiO₂ layer between the electrodes. The recognition of DNA hybridization was accomplished by metallic aggregation of nano-particles. The results showed that DNA is immobilized with nanometer scales and the method for detecting hybridization signals is useful. The experimental results were verified by I-V curves. The conductance between two electrodes changed with the density of the Au-nanoparticles immobilized onto the oxide layer. These results can be applied to the DNA chip and the multi-functional sensors which will be researched in the further study.</P>
Impact of MW, CSR, and RO Satellite Observations on the Korean Integrated Model (KIM) at KMA
Seungwoo Lee,Mee-Ja Kim,Eun-Hee Kim,Hye-Young Kim 한국기상학회 2021 한국기상학회 학술대회 논문집 Vol.2021 No.10
In this study, we investigated the impact of microwave (MW), clear sky radiance (CSR), and radio occultation (RO) data on the improvement in quality of initial analysis and the predictive performance of global numerical model at KMA. Recently, KMA has carried out a task to improve the operational data assimilation system for the performance of KIM model. Several components, such as increased resolution of variational method, update of RTTOV version, newly estimated background error covariances, effective use of radio sonde, and additional ingestion of satellite data, contributed to the improved data assimilation system. Meanwhile, satellite data were the main sources for a significant improvement in the quality of initial fields verified against ECMWF analysis data. We investigated some individual experiments in order to evaluate the relative impact of each satellite observation on the predictive performance of global model. Overall, the RO data showed much higher impact on the improved performance of KIM model than any other satellite observation data. The MW data revealed noticeable benefits to the lower level temperature at late forecast lead time, while the CSR data presented marginal impact on the predictive skills for each synoptic variable.