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      • <i>Litoreibacter</i> <i>ponti</i> sp. nov., isolated from seawater

        Park, Sooyeon,Park, Ji-Min,Park, Doo-Sang,Yoon, Jung-Hoon International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.11

        <P>A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated GJSW-31<SUP>T</SUP>, was isolated from seawater from the South Sea, South Korea. The novel strain grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees, based on 16S rRNA gene sequences, revealed that strain GJSW-31<SUP>T</SUP> clustered with the type strains of species of the genus <I>Litoreibacter</I>. Strain GJSW-31<SUP>T</SUP> exhibited 16S rRNA gene sequence similarity values of 95.2–98.5 % to the type strains of species of the genus <I>Litoreibacter</I> and sequence similarities of less than 96.18 % to type strains of the other species with validly published names. Strain GJSW-31<SUP>T</SUP> contained Q-10 as the predominant ubiquinone and C<SUB>18 : 1</SUB>ω7<I>c</I> as the major fatty acid. The major polar lipids of strain GJSW-31<SUP>T</SUP> were phosphatidylcholine, phosphatidylglycerol, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain GJSW-31<SUP>T</SUP> was 62.5 mol% and its DNA–DNA relatedness values with the type strains of <I>Litoreibacter albidus</I>, <I>Litoreibacter janthinus</I>, <I>Litoreibacter meonggei</I> and <I>Litoreibacter ascidiaceicola</I> were 13–23 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GJSW-31<SUP>T</SUP> is separate from other species of the genus <I>Litoreibacter</I>. On the basis of the data presented, strain GJSW-31<SUP>T</SUP> is considered to represent a novel species of the genus <I>Litoreibacter</I>, for which the name <I>Litoreibacter ponti</I> sp. nov. is proposed. The type strain is GJSW-31<SUP>T</SUP> ( = KCTC 42114<SUP>T</SUP> = NBRC 110379<SUP>T</SUP>).</P>

      • Lutibacter crassostreae sp. nov., isolated from oyster.

        Park, Sooyeon,Park, Ji-Min,Won, Sung-Min,Park, Doo-Sang,Yoon, Jung-Hoon Society for General Microbiology 2015 International journal of systematic and evolutiona Vol.65 No.8

        <P>A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and ovoid or rod-shaped bacterial strain, designated TYO-8T, was isolated from an oyster collected from the South Sea in South Korea. Strain TYO-8T grew optimally at 25?C, at pH?7.0-8.0 and in the presence of 2.0-3.0?% (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, revealed that strain TYO-8T fell within the clade comprising the type strains of species of the genus Lutibacter, clustering coherently with the type strain of Lutibacter litoralis with a sequence similarity of 99.3?%. Strain TYO-8T exhibited 16S rRNA gene sequence similarity values of 95.3-97.5?% to the type strains of other species of the genus Lutibacter and of less than 92.9?% to the type strains of other species with validly published names. Strain TYO-8T contained MK-6 as the predominant menaquinone and iso-C15?:?0, iso-C15?:?0 3-OH and iso-C15?:?1 G as the major fatty acids. The major polar lipids of strain TYO-8T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain TYO-8T was 33.8?mol% and its DNA-DNA relatedness values with the type strains of L. litoralis, Lutibacter aestuarii and Lutibacter flavus were 13-27?%. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain TYO-8T is distinct from other species of the genus Lutibacter. On the basis of the data presented, strain TYO-8T is considered to represent a novel species of the genus Lutibacter, for which the name Lutibacter crassostreae sp. nov. is proposed. The type strain is TYO-8T (?=?KCTC 42461T?=?NBRC 110923T).</P>

      • <i>Gramella aestuariivivens</i> sp. nov., isolated from a tidal flat

        Park, Ji-Min,Park, Sooyeon,Won, Sung-Min,Jung, Yong-Taek,Shin, Kee-Sun,Yoon, Jung-Hoon International Union of Microbiological Societies 2015 International journal of systematic and evolutiona Vol.65 No.4

        <P>A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and rod-shaped or ovoid bacterial strain, designated BG-MY13<SUP>T</SUP>, was isolated from a tidal flat sediment on the South Sea, South Korea. Strain BG-MY13<SUP>T</SUP> grew optimally at 30–35 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain BG-MY13<SUP>T</SUP> falls within the cluster comprising the type strains of species of the genus <I>Gramella</I> . Strain BG-MY13<SUP>T</SUP> exhibited 16S rRNA gene sequ4ence similarity values of 96.9–97.8 % to the type strains of <I>Gramella echinicola</I> , <I>Gramella gaetbulicola</I> , <I>Gramella portivictoriae</I> and <I>Gramella marina</I> and of 94.6–96.5 % to the type strains of other species of the genus <I>Gramella</I> with validly published names. Strain BG-MY13<SUP>T</SUP> contained MK-6 as the predominant menaquinone and iso-C<SUB>15 : 0</SUB> and iso-C<SUB>17 : 0</SUB> 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain BG-MY13<SUP>T</SUP> was 38.9 mol% and DNA–DNA relatedness values with the type strains of <I>G. echinicola</I> , <I>G. gaetbulicola</I> , <I>G. portivictoriae</I> and <I>G. marina </I> were 12–23 %. The differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain BG-MY13<SUP>T</SUP> is separated from other species of the genus <I>Gramella</I> . On the basis of the data presented, strain BG-MY13<SUP>T</SUP> is considered to represent a novel species of the genus <I>Gramella</I> , for which the name <I>Gramella aestuariivivens</I> sp. nov. is proposed. The type strain is BG-MY13<SUP>T</SUP> ( = KCTC 42285<SUP>T</SUP> = NBRC 110677<SUP>T</SUP>).</P>

      • <i>Litorivivens lipolytica</i> gen. nov., sp. nov., a lipolytic bacterium isolated from a tidal flat

        Park, Sooyeon,Park, Ji-Min,Kang, Chul-Hyung,Yoon, Jung-Hoon International Union of Microbiological Societies 2015 International journal of systematic and evolutiona Vol.65 No.1

        <P>A Gram-stain-negative, flagellated, aerobic and rod-shaped or ovoid bacterial strain, designated HJTF-7<SUP>T</SUP>, was isolated from a tidal flat on the South Sea of South Korea, and its taxonomic position was investigated using a polyphasic approach. Strain HJTF-7<SUP>T</SUP> grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees, based on 16S rRNA gene sequences, showed that strain HJTF-7<SUP>T</SUP> joined the cluster comprising the type strains of species of the genera <I>Spongiibacter</I> and <I>Zhongshania</I>. Strain HJTF-7<SUP>T</SUP> exhibited 16S rRNA gene sequence similarities of 90.4–92.5 % to the type strains of species of the genera <I>Spongiibacter</I> and <I>Zhongshania</I> and of less than 91.5 % to the type strains of other recognized species. Strain HJTF-7<SUP>T</SUP> contained Q-8 as the predominant ubiquinone. The major fatty acids were iso-C<SUB>17 : 1</SUB>ω9<I>c</I>, iso-C<SUB>15 : 0</SUB>, iso-C<SUB>17 : 0</SUB>, iso-C<SUB>11 : 0</SUB> 3-OH and C<SUB>17 : 1</SUB>ω8<I>c</I> and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The fatty acid and polar lipid profiles of strain HJTF-7<SUP>T</SUP> were distinct from those of members of the genera <I>Spongiibacter</I> and <I>Zhongshania</I>. The DNA G+C content of strain HJTF-7<SUP>T</SUP> was 55.9 mol%. The phylogenetic data and differential chemotaxonomic and other phenotypic properties revealed that strain HJTF-7<SUP>T</SUP> represents a novel genus and species within the class <I>Gammaproteobacteria</I>, for which the name <I>Litorivivens lipolytica</I> gen. nov., sp. nov. is proposed. The type strain of <I>Litorivivens lipolytica</I> is HJTF-7<SUP>T</SUP> ( = KCTC 42157<SUP>T</SUP> = CECT 8654<SUP>T</SUP>).</P>

      • <i>Aestuariivita boseongensis</i> gen. nov., sp. nov., isolated from a tidal flat sediment

        Park, Sooyeon,Won, Sung-Min,Kim, Hyangmi,Park, Doo-Sang,Yoon, Jung-Hoon International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.9

        <P>A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, BS-B2<SUP>T</SUP>, which was isolated from a tidal flat sediment at Boseong in South Korea, was characterized taxonomically. Strain BS-B2<SUP>T</SUP> grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The novel strain exhibited highest 16S rRNA gene sequence similarity (97.4 %) to <I>Marivita geojedonensis</I> DPG-138<SUP>T</SUP>. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain BS-B2<SUP>T</SUP> is closely related to <I>Primorskyibacter sedentarius</I> KMM 9018<SUP>T</SUP>, showing 96.5 % sequence similarity. Strain BS-B2<SUP>T</SUP> contained Q-10 as the predominant ubiquinone and C<SUB>18 : 1</SUB>ω7<I>c</I> as the predominant fatty acid. The polar lipid profile of strain BS-B2<SUP>T</SUP> comprised phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid as major components, and differentiated it from the type strains of <I>P. sedentarius</I> and <I>M. geojedonensis</I>. The DNA G+C content of strain BS-B2<SUP>T</SUP> was 62.2 mol%. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrated that strain BS-B2<SUP>T</SUP> can be distinguished from phylogenetically related genera as well as <I>P. sedentarius</I> and <I>M. geojedonensis</I>. On the basis of the data presented, strain BS-B2<SUP>T</SUP> is considered to represent a novel species of a new genus, for which the name <I>Aestuariivita boseongensis</I> gen. nov., sp. nov. is proposed. The type strain of <I>Aestuariivita boseongensis</I> is BS-B2<SUP>T</SUP> ( = KCTC 42052<SUP>T</SUP> = CECT 8532<SUP>T</SUP>).</P>

      • Loktanella acticola sp. nov., isolated from seawater

        Park, Sooyeon,Choi, Su Jung,Won, Sung-Min,Yoon, Jung-Hoon Microbiology Society 2017 International journal of systematic and evolutiona Vol.67 No.10

        <P>A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated OISW-6(T), was isolated from seawater near Oido, a South Korean island, and subjected to a polyphasic taxonomic study. Strain OISW-6(T) grew optimally at 25 degrees C, at pH 7.0-8.0 and in the presence of 2.0-3.0% (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OISW-6(T) fell within the clade comprising the type strains of Loktanella species. Strain OISW-6(T) exhibited 16S rRNA gene sequence similarity values of 97.0-98.9% to Loktanellamaricola, Loktanellatamlensis, Loktanellarosea, Loktanellamaritima, Loktanellasediminilitoris and Loktanellalitorea, and of 94.0-96.3% to the type strains of the other Loktanella species. Strain OISW-6(T) contained Q-10 as the predominant ubiquinone and C-18:1 omega 7c as the major fatty acid. The major polar lipids detected in strain OISW-6(T) were phosphatidylcholine, phosphatidylglycerol and one unidentified aminolipid. The DNA G+C content of strain OISW-6(T) was 57.3 mol% and its DNA-DNA relatedness values with the type strains of the six phylogenetically closely related Loktanella species were 8-25 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OISW-6(T) is separated from recognized species of the genus Loktanella. On the basis of the data presented, strain OISW-6(T) is considered to represent a novel species of the genus Loktanella, for which the name Loktanellaacticola sp. nov. is proposed. The type strain is OISW-6(T) (= KCTC 52837(T) = NBRC 112781(T)).</P>

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