Agarose-biofunctionalized, dual-electrospun heteronanofiber mats: toward metal-ion chelating battery separator membranes

Kim, Ju-Myung,Kim, Chanhoon,Yoo, Seungmin,Kim, Jeong-Hoon,Kim, Jung-Hwan,Lim, Jun-Muk,Park, Soojin,Lee, Sang-Young The Royal Society of Chemistry 2015 Journal of Materials Chemistry A Vol.3 No.20

<▼1><P>We demonstrated an agarose-biofunctionalized, dual-electrospun heteronanofiber mat as a new class of chemically active (specifically, metal-ion chelating) separator membranes for high-performance Li-ion batteries.</P></▼1><▼2><P>A facile and efficient way to impart compelling chemical functionality is the utilization of bio-related materials that are easily accessible from natural products. Here, inspired by anomalous physicochemical features and natural abundance of agarose, we demonstrate a new class of agarose-biofunctionalized, dual-electrospun heteronanofiber mats as a chemically active separator membrane for high-performance lithium-ion batteries. The agarose-enabled metal ion chelation effect of the separator membrane, in combination with its highly porous structure and superior electrolyte wettability, provides unprecedented improvement in cell performance far beyond those accessible with conventional battery separator membranes.</P></▼2>

Effect of temperature on single-and mixed-strain fermentation of ruminant feeds

( Seungmin Woo ),( Sooah Kim ),( Suji Ye ),( Soo Rin Kim ),( Jeongman Seol ),( Uyeh Daniel Dooyum ),( Junhee Kim ),( Dong Hyuck Hong ),( Jong Nam Kim ),( Yushin Ha ) 한국축산학회(구 한국동물자원과학회) 2020 한국축산학회지 Vol.62 No.2

Use of raw feedstuffs for livestock is limited by low digestibility. Recently, fermentation of feedstuffs has been highlighted as a new way to improve nutrient absorption through the production of organic acids using inoculated microorganisms, which can also play a probiotic role. However, standard procedures for feedstuff fermentation have not been clearly defined because the process is influenced by climatic variation, and an analytical standard for fermented feedstuffs is lacking. This study aimed to evaluate the microbiological and biochemical changes of feedstuffs during fermentation at temperatures corresponding to different seasons (10℃, 20℃, 30℃, and 40℃). We also investigated the effects of yeast, lactic acid bacteria (LAB), and Bacillus spp. on fermentation and determined the results of their interactions during fermentation. The viable cells were observed within 8 days in single-strain fermentation. However, when feedstuffs were inoculated with a culture of mixed strains, LAB were predominant at low temperatures (10℃ and 20℃), while Bacillus spp. was predominant at high temperatures (30℃ and 40℃). A significant drop in pH from 6.5 to 4.3 was observed when LAB was the dominant strain in the culture, which correlated with the concentrations of lactic acid. Slight ethanol production was detected above 20℃ regardless of the incubation temperature, suggesting active metabolism of yeast, despite this organism making up a marginal portion of the microbes in the mixed culture. These results suggested that fermentation temperature significantly affects microbiological profiles and biochemical parameters, such as pH and the lactic acid concentration, of fermented feedstuffs. Our data provide valuable information for the determination of industrial standards for fermented feedstuffs.

Change of Chemical Properties of Traditional Doenjang Powder During Storage

Seungmin Park,Sunghee Kim,Yunjeong Gwon,Jungeun Kim,Siyul Byeon,Janice Nullan Averilla,Ji Sun Lim,ChanHo Jang,Jisun Oh,Myeong Hui Choi,Jun Young Kim,Jong-Sang Kim 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Correlations between regional characteristics of counties and the ratio of intracounty to extracounty sources of COVID-19 in Gangwon Province, Republic of Korea

Seungmin Jeong,Chaeyun Lim,Sunhak Bae,Youngju Nam,Eunmi Kim,Myeonggi Kim,Saerom Kim,Yeojin Kim 질병관리본부 2023 Osong Public Health and Research Persptectives Vol.14 No.3

Objectives: This study aimed to examine the correlations between the regional characteristics of counties in Gangwon Province, Republic of Korea and the ratio of intracounty to extracounty sources of coronavirus disease 2019 (COVID-19) infection.Methods: The region of the infectious contact was analysed for each COVID-19 case reported in Gangwon Province between February 22, 2020 and February 7, 2022. The population, population density, area, the proportion of urban residents, the proportion of older adults (>65 years), financial independence, and the number of adjacent counties were assessed for each of the 18 counties in Gangwon Province. Correlation coefficients between regional characteristics and the ratio of intracounty to extracounty infections were calculated.Results: In total, 19,645 cases were included in this study. The population, population density, proportion of older adults, and proportion of urban residents were significantly correlated with the ratio of intracounty to extracounty infections. A stratified analysis with an age cut-point of 65 years showed that the proportion of older adults had a significant negative correlation with the ratio of intracounty to extracounty infections. In other words, the proportions of extracounty infections were higher in countries with higher proportions of older adults.Conclusion: Regions with ageing populations should carefully observe trends in infectious disease outbreaks in other regions to prevent possible transmission.

Direct Electrodeposition of Cu on Ru-Al₂O₃ Layer

Kim, Myung Jun,Kim, Hoe Chul,Kim, Soo-Hyun,Yeo, Seungmin,Kwon, Oh Joong,Kim, Jae Jeong The Electrochemical Society 2013 Journal of the Electrochemical Society Vol.160 No.12

<P>This study introduces the direct electrodeposition of Cu on Ru-Al<SUB>2</SUB>O<SUB>3</SUB> layers, which is a promising material to replace Cu seed, and Ta diffusion barrier layers at once. Ru-Al<SUB>2</SUB>O<SUB>3</SUB> layers are deposited by atomic layer deposition (ALD), and their compositions are manipulated by varying the cycle numbers of Ru and Al<SUB>2</SUB>O<SUB>3</SUB> ALD. The addition of Al<SUB>2</SUB>O<SUB>3</SUB> induces the development of nanocrystalline Ru, instead of columnar structure, enhancing the characteristics as a diffusion barrier without losing a role of the seed layer for Cu electrodeposition. The native oxide of Ru is electrochemically eliminated by the coulometric reduction method (CRM) prior to Cu electrodeposition. The influences of both applied potential and the composition of Ru-Al<SUB>2</SUB>O<SUB>3</SUB> layer on Cu film properties are clarified. Furthermore, the ability of Ru-Al<SUB>2</SUB>O<SUB>3</SUB> layer to inhibit Cu diffusion is assessed, and it is confirmed that Ru-Al<SUB>2</SUB>O<SUB>3</SUB> layer perfectly inhibits Cu diffusion during the annealing carried out at the temperature of 550°C for 30 min with the layer thickness of 7.5 nm.</P>

Differential disruption of autoinhibition and defect in assembly of cytoskeleton during cell division decide the fate of human <i>DIAPH1</i>-related cytoskeletopathy

Kim, Bong Jik,Ueyama, Takehiko,Miyoshi, Takushi,Lee, Seungmin,Han, Jin Hee,Park, Hye-Rim,Kim, Ah Reum,Oh, Jayoung,Kim, Min Young,Kang, Yong Seok,Oh, Doo Yi,Yun, Jiwon,Hwang, Sang Mee,Kim, Nayoung K D BMJ Publishing Group Ltd 2019 Journal of medical genetics Vol.56 No.12

<P><B>Background</B></P><P>Diaphanous-related formin 1 (DIA1), which assembles the unbranched actin microfilament and microtubule cytoskeleton, is encoded by <I>DIAPH1</I>. Constitutive activation by the disruption of autoinhibitory interactions between the N-terminal diaphanous inhibitory domain (DID) and C-terminal diaphanous autoregulatory domain (DAD) dysregulates DIA1, resulting in both hearing loss and blood cell abnormalities.</P><P><B>Methods and results</B></P><P>Here, we report the first constitutively active mutant in the DID (p.A265S) of humans with only hearing loss and not blood cell abnormality through whole exome sequencing. The previously reported DAD mutants and our DID mutant (p.A265S) shared the finding of diminished autoinhibitory interaction, abnormally upregulated actin polymerisation activity and increased localisations at the plasma membrane. However, the obvious defect in the DIA1-driven assembly of cytoskeleton ‘during cell division’ was only from the DAD mutants, not from p.A265S, which did not show any blood cell abnormality. We also evaluated the five DID mutants in the hydrophobic pocket since four of these five additional mutants were predicted to critically disrupt interaction between the DID and DAD. These additional pathogenic DID mutants revealed varying degrees of defect in the DIA1-driven cytoskeleton assembly, including nearly normal phenotype during cell division as well as obvious impaired autoinhibition, again coinciding with our key observation in DIA1 mutant (p.A265S) in the DID.</P><P><B>Conclusion</B></P><P>Here, we report the first mutant in the DID of humans with only hearing loss. The differential cell biological phenotypes of DIA1 during cell division appear to be potential determinants of the clinical severity of <I>DIAPH1-</I>related cytoskeletopathy in humans.</P>

Low-Powered pH-Stable Nano-electrokinetically Enhanced Lateral Flow Assay for COVID-19 Antigen Test

Kim Kang Hyeon,유용경,Lee Na Eun,Lee Junwoo,Kim Cheonjung,Lee Seungmin,Kim Jinhwan,Park Seong Jun,Lee Dongtak,이상원,Kim Hyungseok,허돈,Yoon Dae Sung,Lee Jeong Hoon 한국바이오칩학회 2023 BioChip Journal Vol.17 No.3

Lateral fl ow assay (LFA) is a popular diagnostic system used in point-of-care testing (POCT) due to its low cost and portability. However, LFA has limited sensitivity and detection limits, making it challenging to detect low virus titers. Preconcentration through nano-electrokinetic (NEK) techniques have been proposed as a promising solution to improve the sensitivity of LFA. Nevertheless, the acidic conditions used in NEK operations may reduce the specifi city and sensitivity of LFA immunoassays. To address these limitations, an integrated LFA kit, the NEK-enhanced LFA (PcNEK–LFA), has been introduced. This kit features a pH-controlled structure designed to facilitate sample preconcentration. Biomarkers and AuNPs are electrokinetically preconcentrated in the PcNEK–LFA platform to increase the concentration of the test line and Ag–Ab binding events, resulting in enhanced performance. The pH-controlled PcNEK–LFA platform was evaluated using salivary human chorionic gonadotropin beta (β-hCG) and COVID-19 Ag samples, achieving a preconcentrating factor of approximately 10 and a sensitivity enhancement of 55.42%, and a preconcentrating factor greater than 10, respectively. The pH-controlled PcNEK–LFA platform provides an eff ective solution to overcome the limitations of LFA for POCT. In addition, it improves its sensitivity and detection limit, signifi cantly enhancing the accuracy and reliability of POCT, particularly for COVID-19 screening tests. As a result, this platform may play a pivotal role in addressing current and future healthcare challenges, facilitating rapid diagnosis and treatment of infectious diseases.

How knowledge flow affects Korean ICT manufacturing firm performance: a focus on open innovation strategy

Kim, Seungmin,Kim, Hongbum,Kim, Eungdo Carfax Pub. Co 2016 Technology analysis & strategic management Vol.28 No.10

Cell-Type-Specific <i>Shank2</i> Deletion in Mice Leads to Differential Synaptic and Behavioral Phenotypes

Kim, Ryunhee,Kim, Jihye,Chung, Changuk,Ha, Seungmin,Lee, Seungjoon,Lee, Eunee,Yoo, Ye-Eun,Kim, Woohyun,Shin, Wangyong,Kim, Eunjoon Society for Neuroscience 2018 The Journal of neuroscience Vol.38 No.17

<P>Shank2 is an excitatory postsynaptic scaffolding protein implicated in synaptic regulation and psychiatric disorders including autism spectrum disorders. Conventional Shank2-mutant (Shank2(-/-)) mice display several autistic-like behaviors, including social deficits, repetitive behaviors, hyperactivity, and anxiety-like behaviors. However, cell-type-specific contributions to these behaviors have remained largely unclear. Here, we deleted Shank2 in specific cell types and found that male mice lacking Shank2 in excitatory neurons (CaMKII-Cre;Shank2(fl/fl)) show social interaction deficits and mild social communication deficits, hyperactivity, and anxiety-like behaviors. In particular, male mice lacking Shank2 in GABAergic inhibitory neurons (Viaat-Cre;Shank2(fl/fl)) display social communication deficits, repetitive self-grooming, and mild hyperactivity. These behavioral changes were associated with distinct changes in hippocampal and striatal synaptic transmission in the two mouse lines. These results indicate that cell-type-specific deletions of Shank2 in mice lead to differential synaptic and behavioral abnormalities.</P>

시설온실 내부 로봇거치 시스템의 구조해석

우승민 ( Seungmin Woo ),강석호 ( Seokho Kang ),두윰우예다니엘 ( Uyeh Daniel Dooyum ),김영수 ( Yeongsu Kim ),김준희 ( Junhee Kim ),김윤수 ( Yunsu Kim ),김경철 ( Kyoungchul Kim ),이종원 ( Jongwon Lee ),하유신 ( Yushin Ha ) 한국농업기계학회 2019 한국농업기계학회 학술발표논문집 Vol.24 No.2

노동력이 감소하고 있는 농업 현안 문제해결을 위해 로봇화된 스마트팜 기술 개발이 국내외적으로 활발히 추진중에 있다. 그 일환으로 수확 및 이송, 방제 작업에 로봇을 활용하여 시설온실의 완전 무인화 시스템을 구축하는 연구가 진행되고 있다. 시설온실에 적용되는 로봇은 자율 주행형 로봇과 시설온실 내부에 거치되는 겐트리 로봇으로 분류된다. 본 연구에서는 겐트리 로봇이 시설온실 내부에서 X, Y, Z 방면으로 이동할 수 있는 겐트리 로봇 거치 시스템 개발의 기초 연구로서 시설온실 트러스에 설치되어 겐트리 로봇 거치 시스템을 지지하는 브라켓의 두께 및 설치각도에 따른 구조해석을 실시 최적화된 사향을 결정하였다. 내재해형 단동 시설온실 측면 트러스에 적용된 파이프는 일반 구조용 탄소강관(SPS) 재질의 ø 31.8 에 두께 1.4 mm 이다. 여기에 동일한 사향의 파이프 300 mm 를 수평된 방향으로 설치하여 볼팅으로 고정하였으며, 하중의 분산을 위하여 하단에 브라켓을 설치하였다. 브라켓은 시설온실 측면 트러스 파이프와 겐트리로봇 거치 시스템 파이프에 볼팅으로 체결하였다. 구조해석에 적용된 겐트리 로봇 거치 시스템의 하중은 파이프와 형광류 로봇의 무게 및 수확작물의 무게를 고려하여 총 200 kg 으로 설정하였으며, 분산되어 하나의 브라켓에 적용되는 하중 50 kg 이, 브라켓의 두께 및 설치 각도에 따라 겐트리 로봇 거치 시스템에 어떠한 변형량으로 나타나는지 조사하였다. 그 결과 브라켓을 설치하지 않았을 경우 겐트리 로봇 거치 시스템이 아래로 2.16 mm 휘어지는 것으로 나타났으며, 브라켓의 두께가 2 mm, 3 mm, 4 mm 일 경우 각각 0.1718 mm, 0.1550 mm, 0.1482 mm 의 변형량이 나타나 브라켓의 설치 필요성이 입증되었고 그때의 두께는 2 mm 가 적합한 것으로 분석되었다. 설치 각도에 따른 시스템의 항복응력은 45°, 50°, 55°, 60°의 4 수준으로 구분하여 시뮬레이션 하였으며 그 결과 256.11 Mpa, 239.79 Mpa, 226.53 Mpa, 215.67Mpa 로 나타나 설치각도가 50° 일 때 일반 구조용 탄소강관(SPS)의 항복응력점 250 Mpa 미만으로 나타났으며, 55° 에서 60° 일때의 차이점은 크지 않아 55° 일 경우 가장 적합한 것으로 분석되었다. 이상의 시뮬레이션 결과는 겐트리 로봇 거치 시스템 설계에 적용할 것이며, 재현성 실험을 실시하여 유의성 분석을 진행할 것이다.