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Sooah Jang,Hyunjeong Kim,Hyejin Kim,SuKyoung Lee,EunWoo Kim,KeeNam koong,Eosu Kim 대한신경정신의학회 2018 PSYCHIATRY INVESTIGATION Vol.15 No.2
Objective-Conventional methods for organotypic hippocampal tissue slice culture (OHSC) have shown several disadvantages or limitations regarding age of animals used, duration of culture and difficulty using neurodegenerative models. Therefore, we tried to establish OHSC from old 3xTg-Alzheimer’s disease (AD) mice for longer period (over 4 weeks) and to validate utility of this system as a valid platform for translational neuroscience of AD. Methods-OHSC was performed with old 3xTg-AD mice (12-14 months), old wild type mice (12-14 months) and young 3xTg-AD mice (2-4 months) using serum-free medium for 4 weeks. Hippocampal structure was evaluated by 4’, 6-diamidino-2-phenylindole (DAPI) intensity and neuronal metabolism was measured by Alamarblue assay. Pathologic characteristics of AD were also investigated; β-amyloid levels by ELISA, amyloid plaque deposition by Thioflavin-S staining, and glial activation by immunohistochemistry. Results-Following 4-week culture in serum-free media, hippocampal cells and layers were well preserved in cultured slices from old AD mice as was in those from young AD and old wild type mice. On the contrary, excessive regression of total visible cells was observed in conventional serum-containing medium regardless of genotype of mice. In parallel with this well preserved structure, major pathologic characteristics of AD were also well manifested in hippocampal slices from old AD mice. Conclusion-Our findings suggest that long-term OHSC from old 3xTg-AD mouse can serve as a promising ex vivo system for studies on pathophysiology of AD, especially with the minimum number of sacrifice of experimental animals.
Kim, Sooah,Kim, Jungyeon,Song, Ju Hwan,Jung, Young Hoon,Choi, Il‐,Sup,Choi, Wonja,Park, Yong‐,Cheol,Seo, Jin‐,Ho,Kim, Kyoung Heon WILEY‐VCH Verlag 2016 BIOTECHNOLOGY JOURNAL Vol.11 No.9
<P><B>Abstract</B></P><P>Ethanol, the major fermentation product of yeast, is a stress factor in yeast. We previously constructed an ethanol‐tolerant mutant yeast iETS3 by using the global transcriptional machinery engineering. However, the ethanol‐tolerance mechanism has not been systematically investigated. In this study, global metabolite profiling was carried out, mainly by gas chromatography/time‐of‐flight mass spectrometry (GC/TOF MS), to investigate the mechanisms of ethanol tolerance in iETS3. A total of 108 intracellular metabolites were identified by GC/TOF MS and high performance liquid chromatography, and these metabolites were mostly intermediates of the central carbon metabolism. The metabolite profiles of iETS3 and BY4741, cultured with or without ethanol, were significantly different based on principal component and hierarchical clustering analyses. Our metabolomic analyses identified the compositional changes in cell membranes and the activation of glutamate metabolism and the trehalose synthetic pathway as the possible mechanisms for the ethanol tolerance. These metabolic traits can be considered possible targets for further improvement of ethanol tolerance in the mutant. For example, the <I>KGD1</I> deletion mutant, with up‐regulated glutamate metabolism, showed increased tolerance to ethanol. This study has demonstrated that metabolomics can be a useful tool for strain improvement and phenotypic analysis of microorganisms under stress.</P>
김정숙,김지영,김성희,김수아,우정주 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5
Lung cancer is increasing in incidence of cause of death in adult having malignant tumor. Therefore early detection of lung cancer is very important. Recently, screening chest CT scan has been used for early lung cancer detection. However, still simple chest radiograph is the most basic and important examination for detection of lung cancer. Sometimes lung cancer is overlapped with intrathoracic normal structure on chest radiograph, which makes it difficult in detection of the mass. Besides lung cancer was occured in the patient having pulmonary tuberculosis and cancer can be ignored. We have to be used to know atypical feature of lung cancer as well as well known findings on chest radiograph, and then try to decrease the chance of missing lung cancer.
Evaluation and Optimization of Metabolome Sample Preparation Methods for Saccharomyces cerevisiae
Kim, Sooah,Lee, Do Yup,Wohlgemuth, Gert,Park, Hyong Seok,Fiehn, Oliver,Kim, Kyoung Heon American Chemical Society 2013 ANALYTICAL CHEMISTRY - Vol.85 No.4
<P>Metabolome sampling is one of the most important factors that determine the quality of metabolomics data. The main steps in metabolite sample preparation include quenching and metabolite extraction. Quenching with 60% (v/v) cold methanol at −40 °C has been most commonly used for Saccharomyces cerevisiae, and this method was recently modified as “leakage-free cold methanol quenching” using pure methanol at −40 °C. Boiling ethanol (75%, v/v) and cold pure methanol are the most widely used extraction solvents for S. cerevisiae. In the present study, metabolome sampling protocols, including the above methods, were evaluated by analyzing 110 identified intracellular metabolites of S. cerevisiae using gas chromatography/time-of-flight mass spectrometry. According to our results, fast filtration followed by washing with an appropriate volume of water can minimize the metabolite loss due to cell leakage as well as the contamination by extracellular metabolites. For metabolite extraction, acetonitrile/water mixture (1:1, v/v) at −20 °C was the most effective. These results imply that the systematic evaluation of existing methods and the development of customized methods for each microorganism are critical for metabolome sample preparation to facilitate the reliable and accurate analysis of metabolome.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2013/ancham.2013.85.issue-4/ac302881e/production/images/medium/ac-2012-02881e_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac302881e'>ACS Electronic Supporting Info</A></P>
Sooah Kim 서울대학교 사회발전연구소 2020 Journal of Asian Sociology Vol.49 No.4
The purpose of this study is to reveal the discursive structure of Korean society regarding sexual violence through an analysis of news coverage of the Me Too movement, particularly as it regards allegations against former South Chungcheong Province Governor Ahn Hee-jung, and comments left by netizens on portal news sites. Media reports and commenters on news emphasized the vetting of sexual assault victims, exacerbating secondary victimization in this case. The media knowingly presented Ahn’s case devoid of context and actively supported perceptions that “the victim initiated the situation.” Secondary victimization caused by the media and news comment sections is a distinctive phenomenon found in situations related to Korea’s portal services. Negative stereotypes of victims of sexual violence and attitudes that are suspicious of testimonies from victimized women are reproduced into a sense of public opinion, illustrating need for feministic intervention into online comment culture.