3 차원 데이터획득 뇌 FDG-PET 의 정확도 평가

최창운,이경한,최용,임상무,김상은,이정림,홍성운,김병태 대한핵의학회 1999 핵의학 분자영상 Vol.33 No.3

Purpose: To assess the quantitative accuracy and the clinical utility of 3D volumetric PET imaging with FDG in brain studies, 24 patients with various neurological disorders were studied. Materials and Methods: Each patient was injected with 370 MBq of 2-[18F]fluoro-2-deoxy-D-glucose. After a 30 min uptake period, the patients were imaged for 30 min in 2 dimensional acquisition (2D) and subsequently for 10 min in 3 dimensional acquisition imaging (3D) using a GE AdvanceTM PET system. The scatter corrected 3D (3D SC) and non scatter-corrected 3D images were compared with 2D images by applying ROIs on gray and white matter, lesion and contralateral normal areas. Measured and calculated attenuation correction methods for emission images were compared to get the maximum advantage of high sensitivity of 3D acquisition. Results: When normalized to the contrast of 2D images, the contrasts of gray to white matter were 0.75±0.13 (3D) and 0.95±0.12 (3D SC). The contrasts of normal area to lesion were 0.83±0.05 (3D) 0.96±0.05 (3D SC). Three nuclear medicine physicians judged 3D SC images to be superior to the 2D with regards to resolution and noise. Regional counts of calculated attenuation correction was not significantly different to that of measured attenuation correction. Conclusion: 3D PET images with the scatter correction in FDG brain studies provide quantitatively and qualitatively similar images to 2D and can be utilized in a routine clinical setting to reduce scanning time and patient motion artifacts. (Korean J Nucl Med 1999;33:327-36)

Black radish(Raphanus sativus var. niger) inhibits adipogenesis in 3T3L1 cells

Da Wun YANG,Seung Hyun HONG,Seong Eun KIM,Hak Sung LEE,Gi Ok KIM 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.10

Fermented black radish (FBR) ameliorates hepatic lipid accumulation by transcriptional factor Nrf2 activation in HepG2 cells

Seung Hyun Hong,Da Wun Yang,Seong Eun Kim,Hak Sung Lee,Gi Ok Kim 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

부직포 매트의 공극 크기가 오염퇴적물로부터 유기물 및 영양물질의 용출 차단에 미치는 영향 분석

구본운 ( Bon-wun Gu ),홍승희 ( Seung-hee Hong ),박성직 ( Seong-jik Park ) 한국수처리학회(구 한국수처리기술연구회) 2017 한국수처리학회지 Vol.25 No.4

The aim of this study is to evaluate the applicability of non-woven fabric mats (NWFMs) for interrupting organic matters and nutrients from contaminated reservoir sediments and investigate the pore size of NWFMs on its blocking efficiency. In laboratory incubation experiments, environmental conditions (pH, electric conductivity, dissolved oxygen (DO), and oxidation reduction potential) and contaminant concentrations (T-N, NH₄-N, NO₂-N, NO₃-N, T-P, and PO₄-P) were monitored. DO in uncapped condition was exhausted within four days but the DO in NWFM capping was prolonged for more than 22 days. Lower concentration of COD was observed in the NWFMs capping than uncapped, indicating that the NWFMs were effective for interrupting organic matter release. T-N was mainly composed of NH₄-N and NH4-N release was effectively blocked by NWFMs capping. NWFMs capping was effective for interrupting not soluble phosphorus but adsorbed phosphorus release. NWFMs with smaller pore size was effective for blocking the release of T-P and COD than NWFMs with larger pore size, but the influence of pore size on T-N was not significant. It can be concluded that NWFM can be successfully used for remediation of reservoir`s sediments with high amounts of organic matters and nutrients.

서부 경남 지역 감염안내염의 원인균과 임상양상

김병선(Byoung Seon Kim),홍채민(Chae Min Hong),최영제(Young Je Choi),최유진(Yu-Jin Choi),조용운(Yong Wun Cho),유웅선(Woong-Sun Yoo),김성재(Seong-Jae Kim),정인영(In Young Chung) 대한안과학회 2020 대한안과학회지 Vol.61 No.12

목적: 서부 경남 지역 3차 의료기관에서 진단한 감염안내염 환자들의 원인균, 임상양상 및 시력예후를 알아보고자 하였다. 대상과 방법: 2010년 1월부터 2019년 6월까지 경상대학교병원에서 감염안내염으로 진단된 93명(98안)을 대상으로 후향적으로 의무기록을 분석하였으며 모든 환자에서 전방과 유리체 검체의 배양검사를 시행하였고 배양된 균의 종류, 치료 방법, 시력예후 등을 분석하였다. 결과: 대상 환자 98안에서 균이 배양된 경우는 67안(68.4%)이었으며 가장 흔한 감염안내염의 원인은 백내장수술 후 발생한 안내염이었고. 가장 흔한 원인균은 그람양성균인 Staphylococcus epidermidis (16.3%)이었다. 최종 최대교정시력을 1.0 logMAR을 기준으로분석한 결과 초진 최대교정시력이 안전수지 이상인 경우, 유리체절제술을 시행한 경우가 통계적으로 의미있는 예후를 보였다(p<0.001, p=0.021). 결론: 감염안내염 중 외인안내염은 백내장수술 후 많은 발생 빈도를 보이며 그람양성균인 Staphylococcus epidermidis가 가장 흔하며 좋은 시력예후를 보여주었다. 내인안내염은 간농양이 가장 흔한 감염병소이고 그람음성균인 Klebsiella pneumoniae가 흔하고 나쁜 시력예후를 보여주었다. Purpose: To investigate the bacterial culture, clinical characteristics, and prognosis associated with infectious endophthalmitis in western Gyeongsangnam-do. Methods: This study was a retrospective review of the medical records of 98 eyes that presented with infectious endophthalmitis from 2010 to 2019. All patients were analyzed for the type of causative bacteria, treatment method, and the visual prognosis. Results: The bacterial cultures (n = 98 eyes) revealed bacterial growth in 67 eyes (68.4%). The most commonly isolated causative microorganism was Gram-positive Staphylococcus epidermidis (14.3%). As a result of analyzing the best corrected visual acuity (VA) based on 1.0 (logMAR), baseline VA was worse than counting fingers, and whether pars plana vitrectomy was undertaken showed a difference in the final visual acuity (p < 0.001, p = 0.021). Conclusions: Exogenous endophthalmitis occurs frequently after cataract surgery, and S. epidermidis is the most common causative agent, but a good visual prognosis resulted. Liver abscess was a common extraocular source of endogenous endophthalmitis and Klebsiella pneumonia was the most common causative agent for a poor visual prognosis.

Liposomes의 음성염색에 의한 전자현미경적 관찰

유용운,홍성운,김태환,Ryu, Yong-Wun,Hong, Seong-Woon,Kim, Tae-Hwan 한국현미경학회 1989 Applied microscopy Vol.19 No.2

To investigate the research of the tumor seeker using liposomes with negatively stained. The fine structure and size in liposomes composed of PC, DSPC, DAPC and SM phospholipid were observed. And the yield of vesicle affected with sonication by mechanical force were determined. The results were as follows. 1. The stain of 2% UA are obtained a good resolution from electron microscopic observation to compare the negatively liposomes with PTA, AM and UA solution. 2. The fine structure of liposomes except DAPC, PC and CH alone samples could be observed from EM experiment of liposomes composed of PC, DSPC, DAPC and SM phospholipid. 3. The results of experiment from 10, 20, 40 and 60 times sonication with 30sec, prepared 0.1mM NTA-SM-liposomes were obtained unilamella vesicles from groups of multilamella vesicles of phospholipid. 4. About 50nm diameter liposomes obtained through membration filtration step to prepare homogenized liposomes.

PET/CT 감쇠보정시 다양한 CT Kernel 적용에 따른 유용성 평가

이재영,성용준,윤석환,박찬록,이홍재,노경운,Lee, Jae-Young,Seong, Yong-Jun,Yoon, Seok-Hwan,Park, Chan-Rok,Lee, Hong-Jae,Noh, Kyung-Wun 대한핵의학기술학회 2017 핵의학 기술 Vol.21 No.2

PET/CT 영상 재구성시 감쇠보정맵을 사용하여 영상재구성에 적용한다. 감쇠보정 맵의 CT parameter을 변경하여 PET/CT 영상 재구성 할 때 적용하여 SUVmax에 어떤 영향을 미치는지 비교 평가해보고자 한다. 장비는 Biograph mCT 64를 사용하였고 Phantom은 NEMA IEC Body Phantom을 사용하였다. 실험을 위해 환자는 2017년 2월에서 3월까지 본원 PET/CT 검사를 시행한 환자 20명을 대상으로 Lung, Liver, Bone에 관심영역을 선택하여 기존 08f AC, 45f medium, 80f ultra sharp 방식의 CT kernel을 적용한 감쇠보정맵을 사용하여 PET/CT 영상 재구성에 도입 후 방사능 농도(kBq/mL), SUVmax, SD(standard deviation) 변화 유무를 평가하였다. Phantom 방사능 농도 측정 결과 B08f AC 대비 B45f 0.96%, B80f 6.58% 증가하였고 B08f AC 대비 B45f 0.86%, B80f 6.54%각각 증가하였고, SD의 경우 B08f AC 대비 B45f 1.27%, B80f 6.96% 증가하였다. 환자에서 부위별 SUV는 Lung에서 B08f AC 대비 B45f 1.6%, B80f 6.6%, Liver에서 B08f AC 대비 B45f 0.7%, B80f 4.7%, Bone에서 B08f AC 대비 B45f 1.3%, B80f 6.2% 증가를 보였다. 부위별 SD는 Lung에서 B08f AC 대비 B45f 6.2%, B80f 15.4%, Liver에서 B08f AC 대비 B45f 2.1%, B80f 11%, Bone에서 B08f AC 대비 B45f 를 사용할 때 2.3%, B80f 14.7% 증가를 보였다. CT Kernel변화에 따라 sharpness noise와 영상의 질은 변화를 보였으나 SUVmax와 SD는 통계적으로 유의한 차이가 없었다.(P>.05). 핵의학 영상은 정량적인 평가가 중요하다 따라서 부위에 따라 CT kernel이 적절하게 조절되고 noise level이 낮은 감쇠보정 맵을 사용하여 PET/CT 재구성시에 적용하여 정량적 평가에 오류를 줄이는 것이 중요하다고 사료되므로 따라서 같은 부위라 할지라도 sharpness noise가 인위적으로 증가된 kernel을 사용하는 것보다 noise가 낮은 kernel을 사용하는 것이 SD편차를 줄이고 정량적인 평가에 오류를 적게 하여 정확한 진단과 SUV 측정에 유용할 것으로 사료된다. Purpose Recently PET/CT image's attenuation correction is used CTAC(Computed Tomgraphy Attenuation Correction). it can quantitative evaluation by SUV(Standard Uptake Value). This study's purpose is to evaluate SUV and to find proper CT kernel using CTAC with applied various CT kernel to PET/CT construction. Materials and Methods Biograph mCT 64 was used for the equipment. We were performed on 20 patients who had examed at our hospital from february through March 2017. Using NEMA IEC Body Phantom, The data was reconstructed PET/CT images with CTAC appiled various CT kernel. ANOVA was used to evaluated the significant difference in the result. Results The result of measuring the radioactivity concentration of Phantom was B45F 96% and B80F 6.58% against B08F CT kernel, each respectively. the SUVmax increased to B45F 0.86% and B80F 6.54% against B08F CT kernel, In case of patient's parts data, the Lung SUVmax increased to B45F 1.6% and B80F 6.6%, Liver SUVmax increased to B45F 0.7% and B80F 4.7%, and Bone SUVmax increased to B45F 1.3% and B80F 6.2%, respectively. As for parts of patient's about Standard Deviation(SD), the Lung SD increased to B45F 4.2% and B80F 15.4%, Liver SD increased to B45F 2.1% and B80F 11%, and Bone SD increased to B45F 2.3% and B80F 14.7%, respectively. There was no significant difference discovered in three CT kernel (P >.05). Conclusion When using increased noise CT kernel for PET/CT reconstruction, It tends to change both SUVmax and SD in ROI(region of interest), Due to the increase the CT kernel number, Sharp noise increased in ROI. so SUVmax and SD were highly measured, but there was no statistically significant difference. Therefore Using CT kernel of low variation of SD occur less variation of SUV.

용인과 안성 지역의 유기논 및 관행논에서 토양 화학 특성 및 중금속 함량 비교

구본운,이태구,강구,홍성구,홍승길,장태일,김진호,박성직,Gu, Bon-Wun,Lee, Tae-Gu,Kang, Ku,Hong, Seong-Gu,Hong, Seung-Gil,Jang, Tae-Il,Kim, Jin-Ho,Park, Seong-Jik 한국농공학회 2017 한국농공학회논문집 Vol.59 No.3

The aim of this study is to investigate the chemical properties and heavy metal concentration of soils in conventional and organic paddy. We sampled and analyzed topsoil (0~15 cm) and subsoil (15~30 cm) of conventional and organic paddy fields in Yongin and Anseong, South Korea. The statistical significance between groups was determined by Duncan's multiple range test and correlation between soil properties was also analyzed. The results show that organic matter (OM) and T-N of conventional paddy soil were higher than those of organic paddy soil. However, higher T-P concentration was observed in organic paddy soil than conventional paddy soil. As, Pb, and Zn concentration in organic paddy soil were statistically lower than those in conventional paddy soil. The couple of water content (WC) & As, OM & T-N, T-P & $P_2O_5$, T-P & Zn, $P_2O_5$ & Zn, and Cr & Ni had a good positive correlation but the couple of WC & T-P, WC & Zn, T-P & As, and As & Zn had a strong negative correlation. It can be concluded that organic farming is beneficial to soil environment by reducing the amounts of organic matter, T-N, As, Pb, and Zn concentration in paddy soil when compared to conventional farming.

단일광자방출단층촬영 영상에서 산란 및 감쇠 보정에 의한 절대방사능 측정

이정림,최창운,임상무,홍성운 ( Jeong Rim Lee,Chang Woon Choi,Sang Moo Lim,Seong Wun Hong ) 대한핵의학회 1998 핵의학 분자영상 Vol.32 No.4

Purpose: The pvrpose of this study was to evaluate the accuracy of radioactivity quantitation in Tc-99m SPECT by using combined scatter and attenuation correction. Materials and Methods: A cylindrical phantom which simulates tumors (T) and normal tissue (B) was filled with varying activity ratios of Tc-99m. We acquired emission scans of the phantom using a three-headed SPECT system (Trionix, Inc.) witb two energy windows (photopeak window: 126 154 keV and scatter window: 101 123 keV). We performed the scatter correction with dual-energy window subtraction method (k=0.4) and Chang attenuation correction. Three sets of SPECT images were reconstructed using combined scatter and attenuation correction (SC+AC', attenuation correction (AC) and without any correction (NONE). We compared T/B ratio, irnage contrast [(T-B)/(T+B)) and absolute radioactivity with true values. Results: SC+AC images had the highest mean values of T/B ratios. Image contrast was 0.92 in SC+AC, which was close to the true value of 1, and higher than AC (0.77) or NONE (0.80). Errors of true activity by SPECT images ranged from 1 to 11% for SC+AC, 22-47% for AC, and 2 16% for NONE in a phantom which was located 2.4cm from the phantom surface. In a phantom located 10,0cm from the surface, SC+AC underestimated by ?4%, NON.E 40%. However, AC overestimated by 10%. Conclusion: We conclude that accurate SPECT activity quantitation of Tc-99m distribution can be achieved by dual window scatter correc.tion combined with attenuation correction. (Korean J Nucl Med 1998;32:382-90)

기비전 유기논과 관행논의 토양 화학 및 환경 특성 비교

박정수,왕용,강구,구본운,김한중,홍성구,홍승길,박성직,Park, Jeong-Soo,Wang, Long,Kang, Ku,Gu, Bon-Wun,Kim, Han-Joong,Hong, Seong-Gu,Hong, Seung-Gil,Park, Seong-Jik 한국농공학회 2015 한국농공학회논문집 Vol.57 No.6

Organic farming system has been considered environmental friendly and sustainable agricultural practice. However, the influence of organic farming on soil quality and environment is not well informed and controversial. We sampled and analyzed 0~15 cm and 15~30 cm depth soils of organic and conventional paddy fields in Yongin and Anseong. The electric conductivity and organic matter content of organic paddy soil were significantly less (p = 0.0097, 0.0067, respectively) than those of conventional paddy soil. Available phosphate and total phosphorus in 0~15 cm depth of organic paddy soil were $211.1{\pm}135.3$, $872.4{\pm}286.3mg/kg$, respectively, less than those of conventional paddy soil. Available phosphate amount in conventional paddy was $358.8{\pm}246.7mg/kg$, which is higher than 300 mg/kg that can cause secondary environmental contamination by runoff. The amount of total nitrogen in organic paddy soil was less than that in conventional paddy while their difference was not significant. The concentration of the heavy metals in organic paddy soil was also lower than that in conventional paddy soil but their difference was not statistically significant. Our findings demonstrate that electric conductivity, organic matter, nutrients, and heavy metals in organic paddy soil were less than those in conventional paddy soil. However, additional monitoring of soil properties for longer period is necessary to certify such a conclusion.