Discovery of urinary metabolomic biomarkers for early detection of acute kidney injury

Won, A Jin,Kim, Siwon,Kim, Yoon Gyoon,Kim, Kyu-Bong,Choi, Wahn Soo,Kacew, Sam,Kim, Kyeong Seok,Jung, Jee H.,Lee, Byung Mu,Kim, Suhkmann,Kim, Hyung Sik The Royal Society of Chemistry 2016 Molecular bioSystems Vol.12 No.1

<P>The discovery of new biomarkers for early detection of drug-induced acute kidney injury (AKI) is clinically important. In this study, sensitive metabolomic biomarkers identified in the urine of rats were used to detect cisplatin-induced AKI. Cisplatin (10 mg kg(-1), i.p.) was administered to Sprague-Dawley rats, which were subsequently euthanized after 1, 3 or 5 days. In cisplatin-treated rats, mild histopathological alterations were noted at day 1, and these changes were severe at days 3 and 5. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at days 3 and 5. The levels of new urinary protein-based biomarkers, including kidney injury molecule-1 (KIM-1), glutathione S-transferase-alpha (GST-alpha), tissue inhibitor of metalloproteinase-1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, neutrophil, neutrophil gelatinase-associated lipocalin (NGAL), and osteopontin, were significantly elevated at days 3 and 5. Among urinary metabolites, trigonelline and 3-indoxylsulfate (3-IS) levels were significantly decreased in urine collected from cisplatin-treated rats prior to histological kidney damage. However, carbon tetrachloride (CCl4), a hepatotoxicant, did not affect these urinary biomarkers. Trigonelline is closely associated with GSH depletion and results in insufficient antioxidant capacity against cisplatin-induced AKI. The predominant cisplatin-induced AKI marker appeared to be reduced in urinary 3-IS levels. Because 3-IS is predominantly excreted via active secretion in proximal tubules, a decrease is indicative of tubular damage. Further, urinary excretion of 3-IS levels was markedly reduced in patients with AKI compared to normal subjects. The area under the curve receiver operating characteristics (AUC-ROC) for 3-IS was higher than for SCr, BUN, lactate dehydrogenase (LDH), total protein, and glucose. Therefore, low urinary or high serum 3-IS levels may be more useful for early detection of AKI than conventional biomarkers.</P>

저장물의 적재방법에 따른 저온저장고의 열성능 분석

김석우,김성만,현명택,최동호,고정삼,박영하 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

The heat performance in a forced circulating cool storage room was numerically investigated. A commercial PHOENICS package was used to simulate 3-D airflow. For solving the governing equations a standard k-ε turbulent model was implemented to calculate steady state turbulent velocity and temperature distributions. The results show that the large air contacting area of products increases the storage quality, because the airflow between products helps to remove the heat from the products by convective heat transfer.

일부 자동차 공장 근로자들의 職業的 特性과 社會 心理的 Stress의 相關性 硏究

김성삼,홍지우,한현정,최희석,권소희,정해경,김삼태,송용선,이기남 한국전통의학연구소 2004 한국전통의학지 Vol.14 No.1

〈Objective〉 This study were carried out to study to assess the relationship between job characteristics, general characteristics, health behaviors, and psychosocial distress and suggested the preventive oriental medicine approaches in occupational health area. 〈Methods〉 We measured job characteristics(job demand, job control, jop insecurity) by The Karasek's Job Content Questionnaire, health behaviors(smoking, drinking, exercise, coffee), general characteristics at work by a self-administered questionnaire. Psycho social distress was measured by PWI(Psychosocial Well-being Index), a 18-item self- administered instrument. 370 data for research were collected through the process of oriental health examination with questionnaire in workplace. Statistical analysis was done by t test, oneway ANOVA, and hierarchical multiple regression analysis with SPSSWIN (version 10.0) 〈Results〉 Among the 152 respondents, the prevalence of high job strain was 27%(41 persons). The Odds ratio of high job strain was 3.11(95 2.12~4.11), and those of passive group and active group were 2.01(95 0.83~2.76) and 1.80(95 0.83~2.76), respectively. Our results supported the association between psychosocial distress and job strain. 〈Conclusion〉 The odds ratio of high strain isolated group(high strain group+low social support) was 3.59(95 2.05 5.13), but the odds ratio of high strain collective group was 1.32(95 0.23 2.41). Social support at work was modifing factor of the association between psycho social distress and job strain. Therefore we reviewed the evidence that economic depression was associated with psycho social distress in this study, and that the strength of the social support was important in maintain health.

농가용 저온저장고의 제어변수에 따른 성능평가

김석우,김성만,현명택,최동호,고정삼,박영하 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

The low temperature storage method has been used to increase the value of agricultural products by reducing quality deterioration and controlling respiration rate. The respiration rate of agricultural products depends on several factors such as temperature, moisture, gas composition and microbe inside the storage room. Among that temperature is the most important factor, which affects respiration rate and causes severe damage. The results obtained from these experiments are that electric consumption is decreased as setting temperature is decreasing and temperature deviation is increasing. In the ventilation experiment, the case ventilated four times a day(every six hours a day) with ten minutes operation each time, was inspected require as much electric consumption as non-ventilation case.

한국 특수교육정책 변천과정의 분석을 위한 기초연구

이석진,김삼섭 한국특수교육문제연구소 2004 특수교육저널 : 이론과 실천 Vol.5 No.1

이 연구에서는 특수교육정책의 발전을 위하여 한국 특수교육정책의 변천과정을 분석하는데 필요한 분석의 틀을 선행연구 고찰을 통하여 탐색하였다. 그 결과, 한국 특수교육정책의 변천과정을 분석하는데 적절한 틀 혹은 모형의 주요 구성요소는 시대구분, 내용, 형성과정, 집행과정, 평가로 나타났는데, 그 구체적인 내용은 다음과 같다: 첫째, 특수교육정책의 시대구분은 개화기(1870~1911), 일제 강점기(1911~1945), 미군정기(1945~1948), 특수교육정책의 태동기(1948~1977), 특수교육정책의 안정기(1978~1994), 특수교육정책의 발전기(1994~)로 구분 한다; 둘째, 특수교육정책의 내용은 특수교육 계획, 특수교육 행정조직, 특수교육 관련 법규 및 제도, 특수교육 교원정책, 특수교육 교육과정정책 등이다; 셋째, 특수교육정책의 형성과정은 정책요구, 정책문제, 정책논제, 정책의제, 정책공식화, 정책채택, 정책결정의 과정으로 구분 한다; 넷째, 특수교육정책의 집행과정은 공식정책의 내용, 집행기관, 예산의 확보, 갈등, 집행의 변동, 정책집행자의 재량한계 등으로 한다; 다섯째, 특수교육정책의 평가는 입증단계, 타당화단계, 옹호단계, 합리적 선택단계로 구분한다. 이와 같은 연구결과는 추후 한국의 특수교육정책에 관한 연구를 하고자 할 때, 분석의 틀로 적용될 수 있을 것으로 기대된다. The purpose of this research is to investigate a framework that is necessary to analyze the process of changes in Korea special education policies for the development of special education policies. This study was carried out by reviewing papers on policies or educational policies. This research showed that it was reasonable to divide history of policies into the following periods for analysis: the Civilized Nation Period(1870-1911), the Japanese Occupation Period(1911-1945), the American Occupation Period (1945-1948), the Quickening Period of Special Education Policies(1948-1977), the Stable Period of Special Education Policies(1978-1994), and the Developmental Stage of Special Education Policies(1994- ). In the content about the policies of special education, it was appropriate to include the plans of special education, administrative organizations of special education, legislation and systems related to special education, policies of the special education teaching staffs, and policies of the special education curriculum. It was also appropriate to search for (a) the processes of policy demand, policy problems, policy themes, policy discussions, policy formulation, policy adoption, and policy decisions in the formation process of special education policies; and (b) the content of official policies, enforcement organs, guarantee of budget, conflict, changes in enforcement, and discretional limits of enforcers in the process of policy enforcement. Lastly, it was resonable to analyze evaluation of the policies of special education by dividing in the demonstration stage, the validation stage, the supporting stage, and the rational choice stage.

한국 특수교육 행정조직의 발전과정에 관한 연구

이석진,김삼섭 국립특수교육원 2004 특수교육연구 Vol.11 No.2

이 연구는 한국 중앙정부의 특수교육 행정조직의 변천과정을 분석 검토하고, 이를 토대로 특수교육 행정조직의 발전적인 방향을 제시하기 위한 정책발전 연구 중의 하나이다. 이를 위하여 행정조직의 변천과정을 특수교육정책의 태동기·정초기·발전기·도약기로 구분하고, 각 시대의 특수교육 행정조직의 존재여부와 그 기능을 고찰하였다. 연구의 방법은 교육행정 조직의 근거가 되는 정부조직법, 교육인적자원부의 직제연혁 등을 수집하고 이를 시대별로 정리한 후 비교하였다. 연구결과, 특수교육 행정조직의 발전방향은 다음과 같다. 첫째, 교육인적자원부에는 독립된 과 또는 국 단위 이상의 특수교육 행정조직이 있어야 한다. 둘째, 특수교육 행정조직은 특수교육에 관한 전문지식이 있는 전문가들이 장애영역별로 대치되어야 한다. 셋째, 지방 자치단체의 특수교육 행정조직에 관한 연구가 수행되어야 한다. This study reviews the history and transition of the special education administrative system in Korea, and suggests policy-making strategies to give better directions to the Korean special education administrative system. For these purposes, the periodical changes of the special education administrative policy was divided into the followings: The term of each period was classified into the Quickening Period(1870-1948), the Foundation Period(1946-1976), the Developmental Period(1977-1993), and the Take-off period(1994-present). The Quickening Period can be divided into three sub-periods based on the modem history of Korea, Civilized Nation Period(1870-1910), Japanese Occupation Period(1911-1945), and American Military Administration Period(1946-1948). Each period is investigated by the existence and function of the special education administrative policy. The data of this research is collected from the educational administrative organizations based on the National Government Organization Act and the organizational history about the Ministry of Education & Human Resources Development. Comparative data are arranged by the period and analysed. Major suggestions from this study are summarized as follows: First, the special education administrative organizations belonged to the Ministry of Education & Human Resources Development should take more powerful division. Second, expertises with special education knowledge should be stationed by the types of disabilities at the special education administrative organizations. Third, local communities should implement efforts related to the special education administrative organizations.

바터 팽대부에 생긴 유암종 1예

강명주,지삼룡,박석주,이재익,조영완,김준영,박성재,박은택,이연재,이상혁,설상영,배상균 인제대학교 2006 仁濟醫學 Vol.27 No.-

Carcinoid tumors have been reported in a wide range of organs but most commonly involve the lungs, bronchi and gastrointestinal tract. Within the gastrointestinal tract, appendix is the most common location for carcinoid, followed by the distal small intestine, the rectum, and the stomach. Among these, primary involvement of the ampulla of Vater is extremely rare. We report a case of carcinoid tumor of ampulla of Vater. A 62-year-old man presented with epigastric soreness. Gastroduodenal endoscopy showed hyperemic bulging mass on ampulla of Vater and diagnosed as carcinoid tumor by histologic finding, with immunohistochemical study. In ^(111)In-octreoscan, tumor had regional lymph node metastasis, but no evidence of distant metastasis. Pancreatoduodenectomy with lymph node dissection was performed.

FEM을 이용한 선삭가공 절삭조건의 영향 분석

임세환,송춘삼,홍석관,강정진,김주현 한국공작기계학회 2007 한국공작기계학회 추계학술대회논문집 Vol.2007 No.-

A plane strain finite element model is developed to simulate turning with continuous chip formation using adaptive remeshing during the thermal-mechanical coupled analysis. Comparisons between the experimental and simulation results for cutting forces are performed to validate FE analysis results. Influences of machining parameters such as depth of cut, cutting speed and feed rate on cuting force and shear angle are analyzed by Taguchi method. The more depth of cut and feed rate result in the increase of cutting forces, but the rates of increase are decrease due to the larger shear angle. As the cutting speed increases, von Mises stress of workpiece near the tool tip decreases because the strength of specimen decreases at high temperature above 500℃. This thermal effect on cutting force is negligible because the affected area is very localized and the average values of von Mises stress in the shear plane are the same among the diverse cutting parameters.

간세포암의 고주파 열치료 후 천자침 경로를 통한 전이 1례

조영완,박석주,진한영,김준영,이재익,강명주,박정하,윤정희,박성재,지삼룡,이연재,이상혁,설상영 인제대학교 2006 仁濟醫學 Vol.27 No.-

Radiofrequency ablation(RFA), as a form of minimally invasive therapy for hepatocellular carcinoma, has become an important treatment modality. Because of limitation of surgery, RFA has become standard therapy for hepatocellular carcinoma in some situations. But there are some complications of RFA such as bleeding, infection, hematoma, adjacent organ thermal damage including intestinal perforation, needle track seeding, and so on. There are few reports in the literature that systematically evaluate the incidence of needle tract seeding and its associated risk factors. And only 2 cases of needle tract seeding of hepatocellular carcinoma after RFA were reported in Korea. We report a case of needle tract seeding of hepatocellular carcinoma after RFA.

Evaluation of Feed Value of Barley Fodder as an Alternative Feed Ingredient

Kim, Tae-Il,Mayakrishnan, Vijayakumar,Lim, Dong-Hyun,Lee, Hyun-Jeong,Son, Jun-Kyu,Kim, Yoon-Jeong,Choi, Hee-Chul,Shin, Jae-Hyeong,Park, Jong-Ho,Kim, Sam-Churl,Ki, Kwang-Seok The Korean Society of Grassland and Forage Science 2020 한국초지조사료학회지 Vol.40 No.3

Barley is an important cereal gain which is traditionally used in some nations of Asia and North Africa, and there has been growing interest in using barley as an ingredient in food due to their nutritional value and high content of phyto-constituents. However, no study report on comparative feed value between sprouted barley, cornflake and alfalfa hay. Therefore, in this study we aimed to evaluate the chemical composition, amino acid profile and mineral content of 6 day sprouted barley fodder (SBF) compared with cornflake and alfalfa hay using by AOAC method, as an alternative feed ingredient. Results showed that SBF had higher content of crude protein, acid detergent insoluble crude proteins and neutral detergent insoluble crude protein than alfalfa hay and cornflake; cornflake had higher crude fiber, neutral detergent fiber content than SBF and alfalfa hay; alfalfa hay had higher crude fiber, crude ash, acid detergent fiber, neutral detergent fiber and lignin level than SBF and cornflake. Also, significant differences were found on amino acid content among them (p<0.01). The most abundant amino acid in SBF was glutamate (123 g/kg DM), which is higher than in alfalfa hay (1.27%) or cornflake (1.58%). However, methionine (1.33%) and cysteine (1.53%) were the least abundant amino acids in SBF compared with cornflake or alfalfa hay. Furthermore, our study results exhibited that SBE comprise a good sources of minerals including ferrous (90.01 mg/kg) followed by zinc (20.50 mg/kg), magnesium (0.20 mg/kg) and sodium (0.03 mg/kg) as compared to cornflake and alfalfa hay. The present research findings, confirmed that the nutritional values of SBF are comparable to those of cornflake and alfalfa hay. Hence, SBF can be a better alternative feed ingredient for cornflake or alfalfa hay. However, feeding trials will be required to determine acceptability of SBF for ruminant production.