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( Sung Woo Hong ),( Won Hee Hur ),( Yong Ki Lee ),( Jung Eun Choi ),( Sung Woo Kim ),( Tian Zhu Li ),( Jung Hee Kim ),( Sung Min Kim ),( Eun Byul Lee ),( Seung Kew Yoon ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Recent studies have described that cancer stem cell plays a key role radioresistance. The aim of this study is to investigate the molecular and cellular mechanisms of liver cancer stem cell in metastasis after irradiation in HCC. In addition, we found ADAM17 genes associated with liver metastasis that can be used to suppress liver metastasis effectively by molecular regulation. Methods: Both CD133+ and CD133- sorted cells were exposed to γ-irradiation. We investigated the key gene/pathway responsible for metastasis in post-irradiated liver cancer stem cells, CD133+ and CD133- cells using cDNA microarray. Metastatic activities in sorted cells were analyzed by cell migration assay. Also the expressions of the typical genes related metastasis MMP-2 and MMP-9 were also measured by gelatin zymography. Stable cell lines expressing shRNA against human ADAM17 were produced by lentiviral transductions. CD133+ and CD133- cells which were suppressed ADAM17 gene expression were analyzed migration activity. Results: After cDNA microarray analysis, eighty nine metastasis- related genes were up-regulated. Specifically we found that the ADAM17 gene was more increased in CD133+ cells than CD133- cells treated with γ-irradiation. In addition CD133+ cells from radiation exposure were consistently expressed higher levels of vascular endothelial growth factor by ELISA. After irradiation, CD133+ cells migrated more actively, and showed an increased invasion rate compared to CD133- cell. Gelatin zymography showed that MMP-2, -9 proteins expression are significantly higher in CD133+ cells media samples than CD133- cells. Suppression of ADAM17 in CD133+ cells reduced migration activity in treating radiation condition. Conclusions: These results suggest that CD133+ cells have more metastatic capacity than CD133- cells after irradiation. In addition, inhibition of cancer stem cells migration through targeting ADAM17 may be promising the therapeutic against of radiotherapy for HCC.
( Sung Woo Hong ),( Won Hee Hur ),( Jung Eun Choi ),( Young Ki Lee ),( Seung Kew Yoon ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background: Recent studies have described that cancer stem cell plays a key role in radioresistance. The cell surface marker CD133 has been known recently as a cancer stem cell marker expressed in HCC. Recently, ADAM17 was reported to contribute to metastasis. The aim of this study is to investigate the molecular and cellular mechanisms of liver cancer stem cell and ADAM17 in metastasis after irradiation in HCC. Methods: Huh-7CD133+ and Huh-7CD133- sorted cells were exposed to g-irradiation. We investigated the key gene/pathway responsible for metastasis in post-irradiated liver cancer stem cells, CD133+/- cells using cDNA microarray. Metastatic activity was analyzed by cell migration assay. Also the expressions of the MMP-2 and MMP-9 were measured by gelatin zymography. We confirmed ADAM17 gene expression on time dependent by real-time PCR, western blot in sorted cells. ADAM17 is suppressed effectively using ADAM17 lenti virus shRNA in Huh-7 cell line. CD133+/- cells that were suppressed ADAM17 gene expression were analyzed migration activity. Results: After cDNA microarray analysis, eighty nine metastasis related genes were upregulated. Especially we found that the ADAM17 gene was more increased in CD133+ cells than CD133- cells treated with g-irradiation. In addition CD133+ cells from radiation exposure were consistently expressed higher levels of vascular endothelial growth factor by Multiplex cytokine analysis. After irradiation, CD133+ cells migrated more actively, and showed an increased invasion rate compared to CD133- cell. Gelatin zymography showed that MMP-2, -9 expressions are significantly higher in CD133+ cells. Furthermore, shADAM17CD133+ cells reduced migration activity after g-irradiation exposure. Conclusions: These results suggest that CD133+ cells have more metastatic capacity than CD133- cells after treating irradiation. These data further suggest that molecular therapeutic inhibition of cell migration and invasion through ADAM17 suppression may represent a new approach for improving the therapeutic efficacy of radiotherapy for HCC. This work was funded by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2011-0027071).
( Sung Woo Kim ),( Won Hee Hur ),( Kwang Soo Lyoo ),( Jung Eun Choi ),( Sung Woo Hong ),( Young Kee Lee ),( Seung Kew Yoon ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background: NAFLD (nonalcoholic fatty liver disease) has a wide spectrum of liver pathogenesis from simple hepatic steatosis to cirrhosis. NASH (Nonalcoholic Steatohepatitis) is characterized by hepatocyte injury and inflammatory cell infiltration, which is linked with peripheral insulin resistance and increased triglyceride in the liver. Pharmacological properties of oleuropein are anti-oxidant, anti-inflammatory, and anti-ageing. The purposes of this study was to establish an NASH mouse model fed with HFD and to demonstrate the pharmacological effect of oleuropein using the appropriative animal model. Methods: C57BL/6 mice were divided into 3 groups; a normal diet group (ND), a 60% high fat diet group (HFD), 0.05% oleuropein-supplemented high fat diet group (HWO). HWO were fed 0.05% oleuropein-supplemented high fat diet after 6 months. The effect of oleuropein on these models was studied using biochemical, histological and molecular markers. Expression of mRNA level (related adipogenesis, inflammation, and fibrosis) was analyzed by real-time PCR between HFD and HWO. Results: The body weight, total cholesterol, TG, FFA, AST, and ALT values of HFD for 6, 9, and 12 months were higher than that of ND. The NAFLD activity score (NAS) of HFD were increased to inflammation stage at 6 month, compared to ND. HWO was significantly increased to NAS and fibrosis grade, but steatosis grade was not changed. The mRNA levels of LXR, TNF-α and collagen was decreased with treatment period in HWO, but ap2 and α-SMA was not changed. Conclusions: We produced a high fat diet induced NASH model that displayed histopathological features of NAFLD to NASH. The therapeutic effect of oleuropein was prevented with progression of NAFLD to NASH. Therefore, it is speculated that oleuropein may be pharmacologically useful to prevent the progression of steatohepatitis and fibrosis and could be a promising agent of medicine for human NASH. This study was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea. (A090282).
Sang-Woo Hur,Chi-Hoon Lee,Hea-Ja Baek,Choong-Hwan Noh,Sang-Hyun Han,Seung-Bo Oh,Ji-Sung Moon,Young-Don Lee 한국발생생물학회 2014 한국발생생물학회 학술발표대회 Vol.2014 No.9
As a preliminary investigation into the effect of environmental factors control for gonadal development, we examined the involvement of photoperiod and water temperature in ovarian development of Epinephelus. akaara. For the induction of sexual maturation, E. akaara reared in recirculating aquaculture system (RAS). During November 2013, the photoperiod and water temperature was adjusted to 12L:12D and 18℃, respectively. In the photo-thermal treatment group, every 3 weeks daylight was increased as follows a 13L:11D and 14L:10D, and control group was maintained under natural condition. After 9 weeks, water temperature was increased 23℃ in photo-thermal treatment group. The sampled fish every 3 weeks revealed increase in gonadosomatic index (GSI; 5.18±1.38), oocyte diameter and vitellogenic oocytes (423.9±36.1 ㎛) were observed in gonads 12 weeks under photo-thermal treatment group. However, ovarian development was maintained immature stage in control group. In this environmental factors manipulation trial, seventy one of the 95 females (578.4 ± 25.4 g in mean body weight, 31.0 ± 0.5 cm mean total length) treated with HCG injection (doses 500 IU/kg BW) were induced ovulation by artificial stripping. The total volume of ovulated eggs were 3,470 ml and the total volume of fertilized eggs was 3,295 ml. The fertilization rate and hatching rate were 95% and 98%, respectively. These results suggest that the photoperiod as well as water temperature are major environmental factors in triggering the gonadal development of E. akaara.
Evaluation of Changes in Agricultural Stream Water Quality of Small Watershed in Jeonnam Province
Sung-Woo Kim,Sun Kook Kim,Hyeon Ji Kim,So Yeon Lee,Myeong Seok Kim,Jeong-Hwa Kang,Seung-Oh Hur,Soon Ik Kwon 한국토양비료학회 2019 한국토양비료학회지 Vol.52 No.4
This study aimed to identify the changing trends in the agricultural water quality of the major watersheds in Jeonnam Province over a 10-year period ranging from 2007 to 2016. The results of water quality analysis of the study sites revealed that the mean Chemical Oxygen Demand in April, July, and October was 3.20, 3.04, and 2.37 mg L<SUP>-1</SUP>, respectively, with the median values being 2.85, 2.84, and 1.96 mg L<SUP>-1</SUP>, respectively. The mean values of EC (Electrical Conductivity) in April, July, and October were 0.15, 0.15, and 0.17 dS m<SUP>-1</SUP>, respectively. The mean EC values were lower in April and slightly higher in October, and there was no yearly fluctuation. The mean and median values of the T-N (Total Nitrogen) content were 3.41 mg L<SUP>-1</SUP> and 3.18 mgL<SUP>-1</SUP>, respectively. A large deviation in the T-N content was only observed in April 2009 and July 2015. The mean and median values of the T-P (Total Phosphorous) content were 0.09 mg L<SUP>-1</SUP> and 0.05 mg L<SUP>-1</SUP>, respectively. No fluctuations were observed in the T-P content in the months of April, July, and October, during the study period, with the exception of July 2016 and October 2016.