동자개(Pseudobagrus fulvidraco)의 난모세포 성숙과 배란에 대한 스테로이드와 HCG의 in vitro효과

백혜자,한창희,임창구 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

The aim of this study was to determine the effect of steroids and human chorionic gonadotropin(HCG) on in vitro maturation and ovulation of oocyte in Pseudobagrus fulvidraco. Oocytes were incubated in the media Leibovitz L15 supplemented with the various concentration of 17α, 20β-dihydroxy-4-pregnen-3-one (17α20βOHP), 17α-hydroxyprogesterone (17αOHP), progesterone(P₄), estradiol-17β(E₂)and HCG. After 60 hours incubation, the maturation ability of oocyte was assessed by the appearance of germinal vesicle breakdown (GVBD). GVBD was significantly enhanced by the addition of 17α20βOHP, 17αOHP, P₄and HCG(P$lt;0.05). The highest GVBD was observed when 17α20βOHP and HCG were supplemented to media. When oocytes were cultured for 16hours in media containing 10∼1,000 ng/ml 17α20βOHP, 17αOHP and P₄, the rate of GVBD in oocytes cultured in the medium supplemented with 100ng/ml 17α20βOHP (65%) was significantly higher than that with 17αOHP(40%) and P₄(35%). The effects of 17α20βOHP and HCG on GVBD were assessed by various concentration of these hormones. When oocytes were cultured for 60 hours in various media containing 1∼1,000 ng/ml 17α20βOHP or 5∼1,000 IU/ml HCG, the GVBD of oocytes was significantly increased in the medium with 10∼100 ng/ml 17α20βOHP and 500 IU/ml HCG. When oocytes were cultured in the various media supplemented 1∼1,000 ng/ml 17α20βOHP or 5∼1,000 IU/ml HCG for 60 hours, the media with 1∼100 ng/ml 17α20βOHP or 50∼1,000 IU/ml HCG significantly increased in the rate of ovulation. However supplementation with 1,000 ng/ml 17α20βOHP or 5 IU/ml HCG did not improve the rate of ovulation compared to controls. This results indicate that supplementation of steroid and HCG except E₂can improve the in vitro maturation and ovulation of oocyte in P. fulvidraco; HCG and 17α20βOHP may be more effective than other steroids on oocyte maturation and ovulation in P. fulvidraco.

트라이애슬러의 비타민Q 섭취가 호흡순환기능 및 항산화 효소활성에 미치는 영향

현송자,김귀백,박상일,석혜경 東亞大學校附設스포츠科學硏究所 2002 스포츠科學硏究論文集 Vol.20 No.-

The purpose of this present study was to investigate the effect of VitaminQ supplementation on antioxidant enzyme activities and cardiorespiratory function following prolonged exercise in trained triathlers. Male 14 healthy college students of trained triathlers participated in exercise sessions. The subjects were divided into 2 groups, as Non-supplement(NS) or VitaminQ supplement(VQ) groups. VitaminQ subjects were participated in two exercise sessions: an initial prolonged exercise(swim 750m, running 10㎞) and 70%V O2max treadmill running exercise. The Non-supplement group fed normal diet. VitaminQ group adminstated 90㎎/day for 4weeks. Venous blood samples were drown from the forearm antecubital vein pre-supplement, pre-exercise and immediately post-exercise. The results of this study can be summarized as follow: First, the SOD activity of RBC was not changed, while the GPx, GR or GR/GPx activies of Hb were significantly increased following prolonged exercise with CoQ10 supplementation. Second, 70%VO2max treadmill running exercise increased significantly the V O2max and VE, but the heart rate significantly decreased. In conclusion, the present results clearly show that the VitaminQ supplementation gave rise to increase the GPx or GR activies and to improve the cardiorespiratory function.

아마릴 정(글리메피리드 2㎎)에 대한 글리메드 정의 생물학적 동등성

조혜영,박은자,강현아,백승희,이석,김세미,문재동,이용복 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.2

The purpose of the present study was to evaluate the bioequivalence of two glimepiride tables, Amaryl^(?)(Handok/Aventis Pharm. Co., Ltd.) and Glimed (Kuhn Ⅱ Pharm. Co., Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). The glimepiride release from the two glimepiride formulations in vitro was tested using KP Ⅷ Apparatus Ⅱ method with a variety of dissolution media (pH 1.2, 4.0, 6.8 buffer solution, water and blend of PSB 80 into each dissolution medium). Twenty six healthy male subjects, 22.65±2.19 years in age and 66.55±8.85 kg in body weight, were divided into two groups and randomized 2×2 cross-over study was employed. After one tablet containing 2 ㎎ as glimepiride was orally administered, blood was taken at predetermined time intervals and the concentrations of glimepiride in serum were determined using HPLC method with UV detctor. The dissolution profiles of two formulations were similar at all dissolution media. Besides, the pharmacokinetic parameters such as AUC_(t), C_(max) and untransformed T_(max). The results showed that the differences between two formulations based on the Amaryl were -3.70, -8.28 and 0.61% for AUC_(t), C_(max) and T_(max), respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25)(e.g., log(0.84)∼log(1.04) and log(0.82)∼log(1.03) for AUC_(t) and C_(max), respectively). Thus, the criteria of the KFDA guideline for the bioequivalence were satisfied, indicating Glimed tablet and Amaryl tablet were bioequivalent.

8주간 유산소운동이 혈중전해질 농도와 혈중pH에 미치는 영향

현송자,석혜경,김귀백 東亞大學校附設스포츠科學硏究所 2001 스포츠科學硏究論文集 Vol.19 No.-

The purpose of this study is to analyze the electrolyte such as blood ph, Na^(+), K^(+), Ca^(2+), Mg^(2+), etc on the each stage of pre-exercise, post-exercise, each 5 minute-recovery, 10 minute-recovery & 30 minute- recovery after exercise. For this experiment, 14 male students at the department of athletics in B Information Technology college in Busan was classified into each 7 exercise group and 7 non-exercise group, and after training them on the intensity of 60%HRmax of each subject 3 period a week for 8 consecutive weeks, the experiment was carried out with the same condition as training manner in treadmill. The results of this study are as follows. 1. Change of Blood Ph Through Aerobic Exercise In exercise group and non-exercise group, there was no particular change in Blood Ph between rest before training & the rest after 8 week- training according to t test. In case of exercise group, after 8 week-training blood ph value was significantly decreased, and at the 30minute-recovery after exercise, ph value was recovered to the degree of rest (p<.001). In case of non-exercise group, blood ph indicated significant change as the similar tendency showed in exercise group, at 10 minute-recovery after exercise, blood ph was recovered to the degree of rest. (p<.001). The change of ph by period between exercise group and non-exercise group was not significantly different. 2. Change of Blood Electrolyte Through Aerobic Exercise 1) Change of blood Na+ through aerobic exercise In exercise group and non-exercise group, the change of Na^(+) between the rest before training and the rest after training was not significantly different according to the result of t test. In case of exercise group, after performing 8 week-training, Na^(+) value was significantly increased, and at 10 minute-recovery after exercise, Na^(+) value began to recover into the value in rest, and after 30 minute-recovery after exercise, Na^(+) value was recovered into the degree of rest (p<.05). In case of the change of Na^(+) in non exercise group, after exercise, Na' value was significantly increased in comparison with rest period, and at 5 minutes after exercise, it was recovered into the degree of rest. (p<.01). The change of Na^(+) by period between exercise group and non-exercise group was not significantly different. 2) Change of Blood K^(+) through aerobic exercise In exercise group and non-exercise group, the change of K^(+) in rest before training and the rest after training was indicated that there was no significant difference according to t test. In case of exercise group, after 8 week training, K^(+) value was significantly increased, and at 5 minute-recovery after exercise, K^(+) value was recovered into the value in rest (p<.05). K^(+) in non-exercise group was not significantly changed. 3) Change of blood Ca^(+) through aerobic exercise In case of exercise group, the change of Ca^(+) in rest before training and the rest after training was not significantly different according to the result of t test. In case of non-exercise group, the change of Ca^(+) in rest before training and the rest after training was significantly different according to the result of t test (p<.05). In case of exercise group, after 8 week-training, Ca^(+) value was significantly increased, and at 5 minute- ecovery after exercise, Ca^(+) value began to recover into the degree of rest, and after 30 minute-recovery after exercise, Ca^(+) value was recovered to the degree of rest (p<.01). In case of the change of Ca^(+) in non-exercise group, after training, Ca^(+) value was significantly increased in comparison with rest period, and at 30 minute-recovery after exercise, Ca^(+) value was recovered into the degree of rest. (p<.001). In case of the change of Ca^(+) by period between exercise group and non-exercise group, after exercise and at 5 minutes recovery after exercise, the change of Ca^(+) in non-exercise group is higher than that of exercise group (p<.01). 4) Change of blood Mgt^(2+) through aerobic exercise In exercise group and non-exercise group, the change of Mg^(2+) between rest before training and the rest after training was not significantly different according to the result of t test. In both cases of exercise group and non-exercise group, after 8 week- raining, the change of Mg^(2+) was not significantly different. The change of Mg^(2+) by period between exercise group and non-exercise group, after exercise, the change of Mg^(2+) of non-exercise group was higher than that of exercise group (p<.01). 3. Correlation Between Blood Ph and Electolyte Through Aerobic Exercise In performance of 8 week aerobic training, the result of correlation analysis between blood ph and electrolyte in pre-exercise, post-exercise, each 5 minute, 10 minutes, 30 minutes-recovery after exercise showed that in case of Na^(+), K^(+), Mg^(2+) did not have significant correlation, but in case of Ca^(2+), it showed significant negative correlation in pre-exercise and at 10 minute- recovery after exercise. (p<.05).

리스페달 정(리스페리돈 2㎎)에 대한 리스펜 정의 생물학적 동등성

조혜영,박은자,강현아,백승희,이석,박찬호,문재동,이용복 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.2

The purpose of the present study was to evaluate the bioequivalence of two risperidone tablets, Risperdal (Janssen Korea Co., Ltd) and Rispen (Myung In Pharm. Co., Ltd), according to the guidelines of Korea Food and Drug Administration (KFDA). The risperione release from the two risperidone formulations in vitro was tested using KP Ⅷ Apparatus Ⅱ method with various of dissolution media (pH 1.2, 4.0, 6.8 butter solution and water). Twenty four healthy male subjects, 23.33±2.10 years in age and 69.24±8.05 kg in body weight, were divided into two groups and a randomized 2×2 crossover study was employed. After one tablet containing 2 ㎎ as risperidone was orally administered, blood was taken at predetermined time intervals and the concentration of risperidone in serum were determined using HPLC method with UV detector. The dissolution profiles of two formulations were similar at all dissolution media. Besides, the pharmacokinetic parameters such as AUC_(t), C_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_(t), C_(max) and untransformed T_(max). The results showed that the differences between two formulations based on the Risperdal were 0.20, -1.29 and -11.09% for AUC_(t), C_(max) and T_(max), respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25) (e.g., log(0.90)∼log(1.03) and log(0.84)∼log(1.09) for AUC_(t) and C_(max), respectively). Thus, the criteria of the KFDA guideline for the bioequivalence were satisfied, indicating Rispen tablet and Risperdal tablet were bioequivalent.

비타민 E와 C의 복합섭취가 최대운동시 호흡순환기능 및 혈중 지질치에 미치는 영향

현송자,한혜정,석혜경,김귀백 東亞大學校附設스포츠科學硏究所 2001 스포츠科學硏究論文集 Vol.19 No.-

The purpose of this study is to find out the effects of compound supplement vitamins E and C on the blood lipid levels during maximal exercise. Seven subjects are high school students and have to be taken 400IU of vitamin E and 200mg of vitamin C after breakfast during 8weeks, and maximal exercise before vitamin compound supplements and after 8weeks The results of this study are as follows: 1. In maximal exercise before and after taking vitamin E-C compound supplements, oxygen uptake (V02) and METs significantly (P<.01, P<.05) increased. Ventilation (CAE) also increased significantly (P<.05), while heart rate (HR) significantly (P<.05) decreased after vitamin E-C compound supplements. 2. TG was significantly (P<.05, P<.Ol) lowered before as well as after the maximal exercise. Total cholesterol (T-C) showed significant (P<.05) decrease after the maximal exercise. HDL-C indicated significant (P<.05) increase, but LDL-C presented reduction of significance (P<.05) before and after the maximal exercise. The LDL-C/HDL-C ratio significantly (P<.Ol, P<.05) decreased before and following the training. The T-C/HDL-C ratio also retained significant (P<.05, P<.Ol) reduction both before and after the running exercise. In conclusion, the compound supplements of vitamins E and C are considered to improve the respiro-circulatory function in exhaustive exercise, decrease an LDL-C concentration, increase an HDL-C concentration and prevent arteriosclerosis.

비유피-4 정(염산프로피베린 20㎎)에 대한 건일염산프로피베린 정의 생물학적동등성

조혜영,박은자,강현아,백승희,김세미,박찬호,오인준,문재동,이용복 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.5

The purpose of the present study was to evaluate the bioequivalence of two propiverine hydrochloride tablets. BUP-4 (Jeil Pharm. Co., Ltd.) and Kuhnil Propiverine Hydrochloride (Kuhnil Pharm. Co., Ltd.), according to the guidelines of the Korea Food and Drug Administration (KFDA). The propiverine release from the two propiverine hydrochloride formulations in vitro was tested using KP Ⅷ Apparatus Ⅱ method with a variety of dissolution media (pH 1.2, 4.0, 6.8 buffer solutions, water and blend of polysorbate 80 into pH 6.8). Twenty six healthy male subjects, 23.73 ± 2.79 years in age and 67.04 ± 7.93 kg in body weight, were divided into two groups and a randomized 2 x 2 cross-over study was employed. After one tablet containing 20 mg as propiverine hydrochloride was orally administered, blood was taken at predetermined time intervals and the concentrations of propiverine in serum were determined using HPLC method with UV detector. The dis-solution profiles of two formulations were similar at all dissolution media. Besides, the pharmacokinetic parameters such as AUC" C _(max) and T _(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC, C_(max), and untransformed T_(max). The results showed that the differences between two formulations based on the BUP-4 were 0.17%, 7.98% and 4.55% for AUC,, C_(max), and respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically trans-formed data were within the acceptance range of log(0.8) to log(1.25) (e.g., log(0.88)-log(l .12) and log(0.90)-log(l.15) for AUC, and _(max), respectively). Thus, the criteria of the KFDA bioequivalence guideline were satisfied, indicating Kuhnil Propiverine Hydrochloride tablet was bioequivalent to BUP-4 tablet.

스프렌딜 지속정(펠로디핀 5㎎)에 대한 스타핀 지속정의 생물학적동등성

조혜영,강현아,이석,백승희,박은자,최후균,문재동,이용복 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.4

Felodipine is a calcium antagonist that lowers blood pressure by reducing peripheral resistance by means of a direct, selective action on smooth muscle in arterial resistance vessels. Furthermore, it have been approved for the effective in angina pectoris and cardiac failure. The purpose of the present study was to evaluate the bioequivalence of two felodipine extended release (ER) tablets, Splendil (YuHan Corporation) and Stapin (Hana Pharmaceutical Co., Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). THe felodipine release from the two felodipine formulations in vitro was tested using KP Ⅷ Apparatus Ⅱ method at pH 6.5 buffer solution. Twenty six healthy male subjects, 22.73±1.78 years in age and 66.66±7.28 ㎏ in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After two tablets containing 5 ㎎ as felodipine were orally administered, blood sample was taken at predetermined time intervals and the concentrations of felodipine in serum were determined using column-switching HPLC method with UV detector. The dissolution profiles of two formulations were similar at pH 6.5 buffer solution. Besides, the pharmacokinetic parameters such as AUG_(t), C_(max) and T_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_(t) and C_(max) and untransformed T_(max). The results showed that the differences between two formulations based on the Splendil were 2.53%, 1.32% and 18.32% for AUC_(t), C_(max) and T_(mzx), respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25) (e.g., log(0.86)∼log(1.20) and long(0.89)∼long(1.23) for AUC_(t) and C_(max), respectively). Thus, the criteria of the KFDA guidelines for the bioequivalence was satisfied, indicating Stapin ER tablet and Splendil ER tablet are bioequivalent.

범가자미, Verasper variegatus의 난황단백질 분리 및 면역학적 특성

백혜자,한창희,김우진,박중연,김진한,김윤 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

The yolk protein of spotted flounder, Verasper variegatus was purified by precipitation with cold distilled water, followed by Sepharose CL-6B column chromatography. The purified protein was identified as vitellin by Ouchterlony's immunodiffusion test and immunoelectrophoresis. The purified vitellin from ovarian crude extracts has same antigenic determinants with the female specific serum protein, vitellogenin. The molecular weight of purified vitellin was estimated about 550kD by gel filtration. The vitellin was composed of three major subunits with molecular weight of about 108, 85 and 31 kD, and two minor subunits. The vitellin was identified by western blot analysis using anti-vitellin antibody.

범가자미, Verasper variegatus의 생식소 발달단계에 따른 혈중 난황단백전구체 (vitellogenin)와 성 스테로이드 호르몬 변화

한창희,會田勝美,김윤,백혜자,小林牧人 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

Annual plasma levels of vitellogenin and sex steroids were investigated in relation to the gonadal development for understanding the endocrine control of reproduction in spotted flounder, Verasper variegatus. The plasma vitellogenin level was highest, 6.36㎎/㎖, in November when vitellogenesis was most active. The level, thereafter, decreased to 3.81㎎/㎖ in December with the initiation of spawning. On the other hand, estradiol-17 β was highest, 2.7ng/㎖, in December, and rapidly decreased in January when spawning occurred. The decreased level of estradiol-17 β, around 0.2ng/㎖, remained unchanged until May. The profiles of plasma testosterone were similar to those of estradiol-17 β in the fish. The Plasma 17 α-hydroxyprogesterone level was relatively low throughout the spawning period, but increased slightly with the initiation of ovarian development. In males, the plasma testosterone and 11- ketotestosterone were highest in December when spermiation actively proceeded, but rapidly decreased during the spawning period (January).