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Park, Sang-Muk,Lee, Sun-Kyung,Kim, Yoon-Sik Korean Society for Clinical Laboratory Science 2011 대한임상검사과학회지(KJCLS) Vol.43 No.3
The Human Papilloma Virus (HPV) infection has been strongly associated with pathogenesis of uterine cervix carcinoma. HPV type 16, a causative agent of uterine cervix carcinoma, encodes the E6 and E7 oncogenes, expression of which is pivotal for malignant transformation and maintenance of malignant phenotypes. To develop a gene therapy for HPV-related carcinoma, We investigated the effect of E6 short-interfering RNA (E6 siRNA) on the expression of this oncogene and on the growth of HPV 16-related uterine cervix carcinoma cells. SiHa cells, a uterine cervix carcinoma cell line, which contain a single copy of HPV 16 integrated in the chromosome and express the E6 and E7 oncogenes. Before 24 hr of transfection, cells were seeded and transfected with control plasmid or E6 siRNA-expressing plasmid. The mRNA was analysed by reverse transcriptase polymerase chain reaction (RT-PCR). The cell growth rate was investigated by MTT method. The E6 mRNA level in SiHa cells was decreased in HPV 16 E6 siRNA-expression vector transfected cells and a decrease in the growth of these cells was also observed. From these results. it is evident that E6 siRNA played a role in suppression of growth of SiHa cells and has a fair chance as a candidate for gene specific therapy for HPV related uterine cervix carcinoma.
( Sang-muk Park ),( Sun-kyung Lee ),( Yoon-sik Kim ) 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.3
The Human Papilloma Virus (HPV) infection has been strongly associated with pathogenesis of uterine cervix carcinoma. HPV type 16, a causative agent of uterine cervix carcinoma, encodes the E6 and E7 oncogenes, expression of which is pivotal for malignant transformation and maintenance of malignant phenotypes. To develop a gene therapy for HPV-related carcinoma, We investigated the effect of E6 short-interfering RNA (E6 siRNA) on the expression of this oncogene and on the growth of HPV 16-related uterine cervix carcinoma cells. SiHa cells, a uterine cervix carcinoma cell line, which contain a single copy of HPV 16 integrated in the chromosome and express the E6 and E7 oncogenes. Before 24 hr of transfection, cells were seeded and transfected with control plasmid or E6 siRNA-expressing plasmid. The mRNA was analysed by reverse transcriptase polymerase chain reaction (RT- PCR). The cell growth rate was investigated by MTT method. The E6 mRNA level in SiHa cells was decreased in HPV 16 E6 siRNA-expression vector transfected cells and a decrease in the growth of these cells was also observed. From these results. it is evident that E6 siRNA played a role in suppression of growth of SiHa cells and has a fair chance as a candidate for gene specific therapy for HPV related uterine cervix carcinoma.
Park Jinyoung,Ko Young Ho,Choi Won Sang,Hong Sung Jun,Park Kwang Muk,Bang Sun Bae 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.6
Kimchi refrigerators are popular electronic products used by over 90% of Korean households. However, in recent years, because fi res frequently occur in kimchi refrigerators used for more than 15 years, it is urgent to determine the cause and prepare countermeasures. In particular, fi res often occur in kimchi refrigerators produced from 2001 to 2004 by a specifi c manufacturer. In this study, the relay structure was identifi ed as the cause of the problem as it directly infl uenced the fi re. Because the relay’s compressor-driven contact point was directed to the bottom at the electrode’s direction, the arc generated at the contact point when the compressor is turned on or off deteriorated the relay’s insulator. In addition, the metal carbide of the contact point generated by the arc further facilitated the insulator’s deterioration. The continuous deterioration caused a tracking phenomenon and expanded into a fi re. From the results, problems in the design of the relay contacts were confi rmed, and countermeasures were suggested.
Park, Sang In,Lim, Jung Yeon,Jeong, Chang Hyun,Kim, Seong Muk,Jun, Jin Ae,Jeun, Sin-Soo,Il Oh, Won Hindawi Publishing Corporation 2012 Journal of biomedicine & biotechnology Vol.2012 No.-
<P>Numerous studies have shown the benefits of mesenchymal stem cells (MSCs) on the repair of spinal cord injury (SCI) model and on behavioral improvement, but the underlying mechanisms remain unclear. In this study, to investigate possible mechanisms by which MSCs contribute to the alleviation of neurologic deficits, we examined the potential effect of human umbilical cord blood-derived MSCs (hUCB-MSCs) on the endogenous cell proliferation and oligogenesis after SCI. SCI was injured by contusion using a weight-drop impactor and hUCB-MSCs were transplanted into the boundary zone of the injured site. Animals received a daily injection of bromodeoxyuridine (BrdU) for 7 days after treatment to identity newly synthesized cells of ependymal and periependymal cells that immunohistochemically resembled stem/progenitor cells was evident. Behavior analysis revealed that locomotor functions of hUCB-MSCs group were restored significantly and the cavity volume was smaller in the MSCs-transplanted rats compared to the control group. In MSCs-transplanted group, TUNEL-positive cells were decreased and BrdU-positive cells were significantly increased rats compared with control group. In addition, more of BrdU-positive cells expressed neural stem/progenitor cell nestin and oligo-lineage cell such as NG2, CNPase, MBP and glial fibrillary acidic protein typical of astrocytes in the MSC-transplanted rats. Thus, endogenous cell proliferation and oligogenesis contribute to MSC-promoted functional recovery following SCI.</P>
( Sang Muk Oh ),( Chang Gyo Park ),( Joo Hee Kang ),( Eun Jung Kim ),( Hee Youn Chee ),( Bong Ho Lee ),( Kyung Bok Lee ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.3
Dieckol, a polyphenol compound extracted from Brown algae, Ecklonia cava, is known to have anti-inflammatory and anti-tumorigenic activities. However, the underlying molecular mechanism by which dieckol regulates cancer cell growth and proliferation remains elusive. Here we report that dieckol extracted from Ecklonia cava inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced human hepatocellular carcinoma SK-Hep1 cell motility, and that dieckol decreased extracellular signal-regulated kinases 1/2 and c-Jun N-terminal kinases (JNKs) activities, but not p38 Mitogen-Activated Protein Kinase (MAPK), in dose-dependent manner. Dieckol reduced TPA-induced activator protein-1 transcriptional activity. TPA-induced matrix metalloproteinase-9 (MMP-9) activity was greatly reduced in SK-Hep1 cells by treatment of dieckol. These findings suggest that dieckol functions as a potent inhibitor for tumor promoter-mediated MAPK signaling pathways leading to AP-1 and MMP-9 activation thereby regulates cancer cell motility.
Park, Geun Chul,Lee, Seung Muk,Jeong, Sang Hyun,Choi, Jun Hyuk,Lee, Chang Min,Seo, Tae Yang,Jung, Seung-Boo,Lim, Jun Hyung,Joo, Jinho American Scientific Publishers 2016 Journal of nanoscience and nanotechnology Vol.16 No.11
<P>Controllable ZnO architectures with different morphologies and defect states play an important role in the adjustment of the surface area and chemical/ physical properties in metal oxide-based photocatalysts. However, the rapid recombination of the photo-induced electron-hole pairs limits their practical application for the purification of organic pollutions. In the present study, we synthesized 1-D ZnO nanorods (NRs) with a tubular top facet using a facile hydrothermal method, and investigated the effect of growth time on the morphology, chemical bonding state, and photocatalytic activity of the NRs. As the growth time increased, the size (diameter and length) of the NRs slightly increased and the morphology of the top facet varied from a flat to a tubular shape due to the more dominant reaction of dissolution than that of growth. The photocatalytic activity was evaluated based on the degradation of methylene blue in aqueous solution under UV irradiation. The photocatalytic activity of the ZnO NRs increased with increasing growth time, which was attributed to the increased surface area and the reduced defect states (oxygen vacancies) in the NRs. These results suggest that controlling the hydrothermal reaction time is an effective way to tailor the morphology, electronic state, and photocatalytic activity of ZnO nanostructures.</P>
Sung-Hyeon Lee,Jae-Bok Park,Hong-Ju Park,Soo-Muk Cho,Young-Ja Park,Jeong-Im Sin 한국식품영양과학회 2005 Preventive Nutrition and Food Science Vol.10 No.2
Dandelions have been reported to have medicinal properties and bioactive components that impact human health. However, the precise biological properties of dandelions and the parts of the plants possessing bioactive components remain uncertain. In this study, we evaluated 3 different types of dandelions based on their cultivation origin (Songpa, Uiryung, and native Uiryung types) as well as their 4 different plant parts (leaf, flower, root, skin). Each sample was extracted with 80% methanol and then compared for the biological activities (anti-oxidative, immune cell proliferative and tumor cell growth inhibitory activities). All 3 types of dandelions possessed a degree of biological functions including the hydroxyl radical scavenger activity, immune cell proliferative activity and tumor cell growth inhibitory activity. However, there was no significant difference in these activities between the 3 dandelion types. Leaves of all three dandelion types showed the highest levels of all biological activities. To a lesser degree, the flower and root parts displayed biological activities. In the skin parts, anti-oxidative activity was also detected only at higher doses of dandelion extracts. Heating the dandelion leaf extract did not affect the biological activity, suggesting a heat-stable nature of the biological compounds. Taken together, these collective data suggest that dandelions, in particular their leaves, possess a high concentration of heat-resistant biological compounds, which are responsible for anti-oxidative, immune cell proliferative and tumor cell growth-inhibitory activities.
Park, Won-Kun,Moon, Myounghoon,Shin, Sung-Eun,Cho, Jun Muk,Suh, William I.,Chang, Yong Keun,Lee, Bongsoo Elsevier 2018 Algal research Vol.29 No.-
<P><B>Abstract</B></P> <P>The commercial production of microalgae-derived docosahexaenoic acid (DHA) has been expanding due to several advantages of algal over fish oil produced DHA. While the DHA production technology from microalgae is already economically competitive, alternatives to pure glucose and yeast extract still need to be explored to reduce the costs and increase the profits further. In the present study, orange peel waste (OPW) and various nitrogen sources were investigated as alternative nutrient sources for the economic cultivation of <I>Aurantiochytrium</I> sp. KRS101. The utilization of orange peel extract (OPE) supplemented with NaNO<SUB>3</SUB> showed a higher DHA yield than that supplemented with NH<SUB>4</SUB>Cl or urea, and pH5.5 was found to be the optimum initial condition for <I>Aurantiochytrium</I> sp. KRS101. OPE optimized with supplemental NaNO<SUB>3</SUB> (1.2g/L) resulted in a DHA yield of 0.63g/L, which was 2.5 fold greater than the yield obtained using a conventional basal medium containing a similar amount of total nitrogen and 67% greater total carbon sources. This result implied not only that the conventional use of glucose and yeast extract have lower efficiency levels during nutrient metabolism but also that the types of carbon and nutrient sources have a significant effect on the DHA yield. The addition of supplemental glucose further enhanced the biomass, fatty acid methyl esters (FAME), and DHA yields, which unveiled the high C:N ratio requirement of <I>Aurantiochytrium</I> sp. KRS101. These results suggest that the development and optimization of microalgae fermentation using OPE and NaNO<SUB>3</SUB> is a possible route for the economical production of DHA and for the additional utilization of food waste.</P> <P><B>Highlights</B></P> <P> <UL> <LI> OPE (orange peel extract) was used for the cultivation of DHA-producing microalgae. </LI> <LI> NO3− was the most effective inorganic nitrogen source for DHA production. </LI> <LI> Supplemental glucose further increased the biomass and lipid productivity. </LI> <LI> OPE showed superior DHA production performance compared to conventional media. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>