환자의 비강으로부터 분리된 메티실린 내성 황색 포도알균의 분자 아형 분석

Sang-Ha Kim,Sung-Bae Park,Heechul Park,Jun Seong Kim,Jungho Kim,Jiyoung Lee,임재원,Young Kwon Kim,Sunghyun Kim 대한임상검사과학회 2020 대한임상검사과학회지(KJCLS) Vol.52 No.2

Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium that causes infections in different parts of the body and causes skin and soft tissue infections (SSTI). The present study examined the antimicrobial resistance patterns and molecular epidemiological characteristics of MRSA isolated from nasal swabs in clinical patients. SCCmec type of MRSA isolates from clinical patients were analyzed: 24 cases were SCCmec type-II; two cases were type-II/IVa; one case was type-II/V; one case was type-IVa; 11 cases were not-typeable. The mec complex type of MRSA isolates from clinical patients were analyzed: 29 cases were mec complex type A, and 10 cases were not-typeable, but type B was not found in the present study. In conclusion, SCCmec type-II and mec complex type A were the most dominant MRSA subtypes among the MRSA isolates from a nasal swab of patients, and the results were similar to other studies on hospital-acquired MRSA (HA-MRSA). These results can not only provide basic data for hospital infection management but also be a good guideline for MRSA infections in the Republic of Korea. 본 연구에서는 환자의 비강으로부터 분리 배양된 총 39건의 MRSA 분리 배양 균주를 이용해 mecA 유전자 검출, SCCmec typing과 mec complex typing을 분석해 보고자 하였다. 임상 환자의 비강으로부터 분리 배양된 MRSA 총 39주 중 SCCmec type-II가 24건, type-II/IVa가 2건, type-II/V가 1건, type-IVa가 1건, not-typeable이 11건으로 분석되었으며, mec complex type A가 29건, not-typeable이 10건이었으며, type B는 없는 것으로 분석 되었다. 결론적으로, 환자의 비강으로부터 분리된 MRSA 분리 배양 균주 중 SCCmec type-II와 mec complex type A 아형이 가장 많이 분포하고 있었으며, 이 결과는 의료기관 획득 MRSA에 관한 다른 연구결과와 유사한 결과를 나타냈다. 이후 환자의 비강으로부터 분리 배양된 not-typeable 아형의 MRSA 균주를 대상으로 국내에서 발견되는 새로운 MRSA 아형 규명에 관한 추가 연구가 필요할 뿐만 아니라, MRSA 분리 배양 균주의 아형을 분석함으로써 그 분자적 특성을 분석한 결과를 바탕으로 병원 감염관리를 위한 기초자료를 제공할 수 것으로 사료된다.

Bloodstream Infections and Clinical Significance of Healthcare-associated Bacteremia: A Multicenter Surveillance Study in Korean Hospitals

Son, Jun Seong,Song, Jae-Hoon,Ko, Kwan Soo,Yeom, Joon Sup,Ki, Hyun Kyun,Kim, Shin-Woo,Chang, Hyun-Ha,Ryu, Seong Yeol,Kim, Yeon-Sook,Jung, Sook-In,Shin, Sang Yop,Oh, Hee Bok,Lee, Yeong Seon,Chung, Doo The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.7

<P>Recent changes in healthcare systems have changed the epidemiologic paradigms in many infectious fields including bloodstream infection (BSI). We compared clinical characteristics of community-acquired (CA), hospital-acquired (HA), and healthcare-associated (HCA) BSI. We performed a prospective nationwide multicenter surveillance study from 9 university hospitals in Korea. Total 1,605 blood isolates were collected from 2006 to 2007, and 1,144 isolates were considered true pathogens. HA-BSI accounted for 48.8%, CA-BSI for 33.2%, and HCA-BSI for 18.0%. HA-BSI and HCA-BSI were more likely to have severe comorbidities. <I>Escherichia coli</I> was the most common isolate in CA-BSI (47.1%) and HCA-BSI (27.2%). In contrast, <I>Staphylococcus aureus</I> (15.2%), coagulase-negative <I>Staphylococcus</I> (15.1%) were the common isolates in HA-BSI. The rate of appropriate empiric antimicrobial therapy was the highest in CA-BSI (89.0%) followed by HCA-BSI (76.4%), and HA-BSI (75.0%). The 30-day mortality rate was the highest in HA-BSI (23.0%) followed by HCA-BSI (18.4%), and CA-BSI (10.2%). High Pitt score and inappropriate empirical antibiotic therapy were the independent risk factors for mortality by multivariate analysis. In conclusion, the present data suggest that clinical features, outcome, and microbiologic features of causative pathogens vary by origin of BSI. Especially, HCA-BSI shows unique clinical characteristics, which should be considered a distinct category for more appropriate antibiotic treatment.</P>

Suppression of lung inflammation by the methanol extract of <i>Spilanthes acmella</i> Murray is related to differential regulation of NF-κB and Nrf2

Kim, Kyun Ha,Kim, Eun Jung,Kwun, Min Jung,Lee, Ji Yeon,Bach, Tran The,Eum, Sang Mi,Choi, Jun Yong,Cho, Sayeon,Kim, Sang-Jun,Jeong, Seung-Il,Joo, Myungsoo Elsevier 2018 Journal of Ethnopharmacology Vol.217 No.-

<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P>Although <I>Spilanthes acmella</I> has been used to relieve inflammation, fever, pain, or infection in traditional Asian medicine, experimental evidence supporting these functions is scarce. Here, we examined an anti-inflammatory function and a possible underlying mechanism of <I>S. acmella</I> Murray (SAM).</P> <P><B>Materials and method</B></P> <P>The methanol extract of SAM was fingerprinted by HPLC. C57BL/6 mice were administered with a single intratracheal (i.t.) LPS and 2 h later with a single i.t. SAM. The effect of SAM on lung inflammation was assessed by histology, semi-quantitative RT-PCR, and MPO assay of lung tissue. The effects of SAM on a pro-inflammatory factor NF-κB and an anti-inflammatory factor Nrf2 were analyzed by immunoblotting of nuclear proteins and by semi-quantitative RT-PCR analysis of mRNA of the genes governed by these transcription factors. V5-Nrf2 was precipitated by an anti-V5 antibody and the ubiquitinated V5-Nrf2 was revealed by immunoblotting of HA-tagged ubiquitin.</P> <P><B>Results</B></P> <P>The i.t. SAM robustly diminished a neutrophilic lung inflammation induced by i.t. LPS treatment of mice. In RAW 264.7 cells, SAM suppressed the nuclear localization of NF-κB and the expression of NF-κB-dependent cytokine genes. SAM increased the level of Nrf2 in the nucleus and the expression of Nrf2-dependent genes while suppressing ubiquitination of Nrf2.</P> <P><B>Conclusion</B></P> <P>Our results suggest that SAM can suppress a neutrophilic inflammation in mouse lungs, which is associated with suppressed NF-κB and activated Nrf2. Our results provide experimental evidence supporting the anti-inflammatory function of <I>S. acmella</I>.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

분산형 지역 기반 이동성 IP

김기일(Ki-Il Kim),이준화(Jun-Hwa Lee),김상하(Sang-Ha Kim) 한국정보과학회 2000 한국정보과학회 학술발표논문집 Vol.27 No.1A

지금까지 인터넷에서 단말기의 이동성을 보장하기 위한 연구 방향은 계층적 망 구조에 따라 매크로 이동성과 마이크로 이동성을 분리하는 것이다. 핸드오프가 적은 매크로 이동성의 경우 Mobile IP로 수렴되는 반면 이동성이 잦은 마이크로 이동성의 경우 HA로 보내지는 제어 메시지를 어떻게 줄일 것인가에 대하여 서로 다른 방안이 제시되고 있다. 현재까지 제시된 연구의 공통적인 문제는 게이트웨이 라우터의 집중화로 발생할 수 있는 부하이다. 본 논문에서는 단말기의 이동의 지역성 및 분산된 지역 에이전트 개념을 도입하여 이러한 문제를 개선한 분산 지역 기반 이동 프로토콜인 DRMIP를 제안한다. 또 DRMIP의 특성 및 적용성을 검증하기 위하여 인터넷 프로토콜과의 호환성, QoS 지원성, 시스템 안정성, 광역단위로의 확장성 등의 측면에서 타 프로토콜과 비교 분석하였다.

Clinical and Genetic Characteristics of Retinal Capillary Hemangioblastoma in Korean Patients

Sang Ha Lee(Sang Ha Lee),Kyu Hyung Park(Kyu Hyung Park),Se Joon Woo(Se Joon Woo),Sang Jun Park(Sang Jun Park),Kwangsic Joo(Kwangsic Joo) 대한안과학회 2022 Korean Journal of Ophthalmology Vol.36 No.6

Purpose: We investigated the clinical features of Korean patients with retinal capillary hemangioblastoma (RCH) and genetic variants of the von Hippel-Lindau (VHL) gene. Methods: A retrospective analysis was performed on patients with RCH from 2003 to 2021 at Seoul National University Bundang Hospital. Sporadic and hereditary RCH associated with VHL disease were classified based on the specific tumors and family history. Clinical features, including the location and number of RCH and bilateral involvement, were investigated. Multiplex ligation-dependent probe amplification and direct sequencing targeting the VHL gene were performed for six RCH cases associated with VHL disease. Results: A total of 18 patients (23 eyes) were enrolled in this study. The mean age at diagnosis was 37 ± 15 years. Twelve patients had hereditary RCH associated with VHL disease, and six patients had sporadic RCH. All five patients with bilateral RCH were clinically diagnosed with VHL disease, and 13 patients had unilateral RCH. Juxtapapillary RCH was only observed in patients with VHL. The most common complication of RCH was the epiretinal membrane, followed by the subretinal fluid. Pathogenic variants were identified in four patients. All three patients with type 1 VHL had the well-known missense mutation p.Glu70Lys, and one patient with type 2 VHL had the nonsense mutation p.Trp88Ter. Conclusions: In Korean patients with RCH, bilateral involvement and juxtapapillary RCH are highly likely to be associated with VHL disease. Because RCH may be the first clinical manifestation in patients with VHL, active genetic testing of the VHL gene and systemic evaluation are required.

Analysis of high-fat diet-induced inflammatory responses in Rhbdf2 knockout mice

Sung-Jun Kim,Ki-Hoan Nam,Seul-Gi Park,Young-Sub Byun,Eun-Kyoung Kim,Sang-Mi Cho,Ha-rim-Kim,Hyoung-Chin Kim,Hu-Jang Lee,Beom Jun Lee 한국예방수의학회 2018 예방수의학회지 Vol.42 No.4

This study investigated the characteristics of obesity induced by a high-fat diet (HD) over 13 weeks in Rhbdf2 gene knockout (KO) mice. Forty 7-week-old Rhbdf2 wild and KO mice were used and the mice were divided into 4 groups: Wild-ND (n=10, Rhbdf2 wild mice, normal diet (ND)), Wild-HD (n=10, Rhbdf2 wild mice, HD), KO-ND (n=10, Rhbdf2 KO mice, ND) and KO-HD (n=10, Rhbdf2 KO mice, HD). The relative epididymal fat weight in KO-HD was significantly increased compared with that in KO-ND (P<0.01). The relative liver and spleen weights in KO-HD were decreased compared with those in Wild-HD (p < 0.05) and KO-ND (p < 0.01). The mRNA expression of SOD1 in KO-ND was significantly reduced compared with that in Wild-ND (p < 0.05). In Wild-ND and HD, the mRNA expressions of TNF-α and IL-6 in epididymal fat were significantly increased compared with those in KO-ND and HD (p < 0.01). A significant increase of TNF- α and IL-6 mRNA expression was observed in KO-HD compared with KO-ND (p < 0.01). These results indicated that Rhbdf2 genes may regulate high fat diet-induced obesity damage by anti-inflammatory and anti-oxidative roles in fat tissue of mice.

Highly stable carbon nanotube field emitters on small metal tips against electrical arcing

Ha, Jun Mok,Kim, Hyun Jin,Raza, Hamid Saeed,Cho, Sung Oh Springer 2013 NANOSCALE RESEARCH LETTERS Vol.8 No.1

<P>Carbon nanotube (CNT) field emitters that exhibit extremely high stability against high-voltage arcing have been demonstrated. The CNT emitters were fabricated on a sharp copper tip substrate that produces a high electric field. A metal mixture composed of silver, copper, and indium micro- and nanoparticles was used as a binder to attach CNTs to the substrate. Due to the strong adhesion of the metal mixture, CNTs were not detached from the substrate even after many intense arcing events. Through electrical conditioning of the as-prepared CNT emitters, vertically standing CNTs with almost the same heights were formed on the substrate surface and most of loosely bound impurities were removed from the substrate. Consequently, no arcing was observed during the normal operation of the CNT emitters and the emission current remained constant even after intentionally inducing arcing at current densities up to 70 mA/cm<SUP>2</SUP>.</P>

Determination of Optimal Growing Degree Days and Cultivars of Kimchi Cabbage for Growth and Yield during Spring Cultivation under Shading Conditions

Ha Seon Sim,Won Jun Jo,Hye Jin Lee,Yu Hyun Moon,Ui Jeong Woo,Soo Bin Jung,Su Ran Ahn,Sung Kyeom Kim 한국원예학회 2021 원예과학기술지 Vol.39 No.6

This study determined the optimal growing degree days (GDD) and cultivars of Kimchi cabbage (Brassica rapa L. ssp. pekinensis) for growth and yield during spring cultivation under shading condition. There were nine treatments, and the experiments were conducted with three GDD levels (899, 924, and 961) and three cultivars (‘Chungwang’, ‘Cheongna’, and ‘Cheongok’) under shading conditions. The process-based model of Kimchi cabbage was applied for growth and yield prediction using meteorological data regarding light, temperature, wind, precipitation, and humidity in several regions. The number of leaves of ‘Chungwang’ Kimchi cabbage was greater than that of ‘Cheongna’ and ‘Choengok’ at all planting times. Most of the growth parameters were highest in the delayed transplanting treatment than in the other transplanting times. ‘Chungwang’ Kimchi cabbage was less sensitive to high temperature during spring cultivation than the other cultivars. GDD 924 was the most optimal time to harvest under the shading condition. GDD 750-870 is considered an appropriate harvest time in the primary production regions during the spring cultivation season. If shading is performed in these regions, constant production may be possible, thus, alleviating damage from high temperature.

A Predictive Dynamic Model Describing the Effect of Relative Humidity on E. coli O157:H7 Growth in Cabbage at 15℃

Jun Wang,Tian Ding,S. M. E. Rahman,Na-Jung Choi,Ha-Na Kim,Jae-ho Choi,Uranchimeg Purev,Sang-Do Ha,Gyung-Jin Bahk,Myung-Sub Chung,Hyang-Sook Chun,Deog-Hwan Oh 동아시아식생활학회 2010 동아시아식생활학회 학술발표대회논문집 Vol.2010 No.11

Reputation Mechanism to Support Agent-Based Services Reliability

Sung-Jun Na,Dong-Uk Kim,Gun-Ha Lee,Dong-Ryeol Shin 대한전자공학회 2007 ITC-CSCC :International Technical Conference on Ci Vol.2007 No.7

In this paper, we analyze the DF’s problem and propose the solution. We describe the reputation mechanism for detecting these fake services. Reputation mechanisms assume the presence of other agent who can provide ratings for other agents that are reflective of the performance or behavior of the corresponding agents.